Monoseeding improves stand establishment through regulation of apical hook formation and hypocotyl elongation in cotton

Water pumping restrictions of high-quality irrigation water from underground aquifers is affecting vegetable production in Southwest Texas. There is a need to develop efficient deficit-irrigation strategies to minimize irrigation inputs and maintain crop profitability. Our objective was to determine how growth, yield, and quality of cantaloupe (Cucumis melo L. cv. `Caravelle') are affected by irrigation systems with varying input levels, including drip depth position and polyethylene mulch. Stand establishment systems used were containerized transplants and direct seeding. Field experiments were conducted on a Uvalde silty clay loam soil. Marketable yields increased in the order of pre-irrigation followed by: dry-land conditions, furrow/no-mulch, furrow/mulch, drip-surface (0 cm depth)/mulch, drip-subsurface (10-cm depth)/mulch, and drip-subsurface (30 cm depth)/mulch. Pooled across all drip depth treatments, plants on drip had higher water use efficiency than plants on furrow/no-mulch or furrow/mulch systems. Transplants with drip-surface produced 75% higher total and fruit size No. 9 yields than drip-subsurface (10- or 30-cm depth) during the first harvest, but total yields were unaffected by drip tape position. About similar trends were measured in a subsequent study except for a significant irrigation system (stand establishment interaction for yield. Total yields were highest for transplants on drip-subsurface (10-cm depth) and direct seeded plants on drip-subsurface (10 and 30 cm depth) with mulch.

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LecRK-Ⅷ.2 mediates the cross-talk between sugar and brassinosteroid during hypocotyl elongation in Arabidopsis

Reproduction and Breeding ◽

10.1016/j.repbre.2021.03.004 ◽

2021 ◽

Vol 1 (1) ◽

pp. 55-63

Author(s):

NingJie Hao ◽

Xiaoxiao Zou ◽

Xiaoxia Lin ◽

Ruqiong Cai ◽

Wenjun Xiao ◽

...

Keyword(s):

Cross Talk ◽

Hypocotyl Elongation ◽

The Cross

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The microtubule-associated protein WDL4 modulates auxin distribution to promote apical hook opening in Arabidopsis

The Plant Cell ◽

10.1093/plcell/koab080 ◽

2021 ◽

Author(s):

Jia Deng ◽

Xiangfeng Wang ◽

Ziqiang Liu ◽

Tonglin Mao

Keyword(s):

Cell Growth ◽

Plant Cell ◽

Positive Role ◽

Apical Hook ◽

Auxin Distribution ◽

Differential Cell ◽

Dicotyledonous Plants ◽

Underlying Mechanisms ◽

Temporal Expression Pattern ◽

Plant Cell Growth

AbstractThe unique apical hook in dicotyledonous plants protects the shoot apical meristem and cotyledons when seedlings emerge through the soil. Its formation involves differential cell growth under the coordinated control of plant hormones, especially ethylene and auxin. Microtubules are essential players in plant cell growth that are regulated by multiple microtubule-associated proteins (MAPs). However, the role and underlying mechanisms of MAP-microtubule modules in differential cell growth are poorly understood. In this study, we found that the previously uncharacterized Arabidopsis MAP WAVE-DAMPENED2-LIKE4 (WDL4) protein plays a positive role in apical hook opening. WDL4 exhibits a temporal expression pattern during hook development in dark-grown seedlings that is directly regulated by ethylene signaling. WDL4 mutants showed a delayed hook opening phenotype while overexpression of WDL4 resulted in enhanced hook opening. In particular, wdl4-1 mutants exhibited stronger auxin accumulation in the concave side of the apical hook. Furthermore, the regulation of the auxin maxima and trafficking of the auxin efflux carriers PIN-FORMED1 (PIN1) and PIN7 in the hook region is critical for WDL4-mediated hook opening. Together, our study demonstrates that WDL4 positively regulates apical hook opening by modulating auxin distribution, thus unraveling a mechanism for MAP-mediated differential plant cell growth.

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The CBL-Interacting Protein Kinase NtCIPK23 Positively Regulates Seed Germination and Early Seedling Development in Tobacco (Nicotiana tabacum L.)

Plants ◽

10.3390/plants10020323 ◽

2021 ◽

Vol 10 (2) ◽

pp. 323

Author(s):

Sujuan Shi ◽

Lulu An ◽

Jingjing Mao ◽

Oluwaseun Olayemi Aluko ◽

Zia Ullah ◽

...

Keyword(s):

Protein Kinase ◽

Seed Germination ◽

Seedling Growth ◽

Hypocotyl Elongation ◽

Interacting Protein ◽

Knock Out ◽

Early Seedling Growth ◽

Tobacco Seedlings ◽

Early Seedling ◽

Cotyledon Expansion

CBL-interacting protein kinase (CIPK) family is a unique group of serine/threonine protein kinase family identified in plants. Among this family, AtCIPK23 and its homologs in some plants are taken as a notable group for their importance in ions transport and stress responses. However, there are limited reports on their roles in seedling growth and development, especially in Solanaceae plants. In this study, NtCIPK23, a homolog of AtCIPK23 was cloned from Nicotiana tabacum. Expression analysis showed that NtCIPK23 is mainly expressed in the radicle, hypocotyl, and cotyledons of young tobacco seedlings. The transcriptional level of NtCIPK23 changes rapidly and spatiotemporally during seed germination and early seedling growth. To study the biological function of NtCIPK23 at these stages, the overexpressing and CRISPR/Cas9-mediated knock-out (ntcipk23) tobacco lines were generated. Phenotype analysis indicated that knock-out of NtCIPK23 significantly delays seed germination and the appearance of green cotyledon of young tobacco seedling. Overexpression of NtCIPK23 promotes cotyledon expansion and hypocotyl elongation of young tobacco seedlings. The expression of NtCIPK23 in hypocotyl is strongly upregulated by darkness and inhibited under light, suggesting that a regulatory mechanism of light might underlie. Consistently, a more obvious difference in hypocotyl length among different tobacco materials was observed in the dark, compared to that under the light, indicating that the upregulation of NtCIPK23 contributes greatly to the hypocotyl elongation. Taken together, NtCIPK23 not only enhances tobacco seed germination, but also accelerate early seedling growth by promoting cotyledon greening rate, cotyledon expansion and hypocotyl elongation of young tobacco seedlings.

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