Impact of blastocyst quality at time of cryopreservation on frozen embryo transfer (FET) success rates is dependent on patient age

2009 ◽  
Vol 92 (3) ◽  
pp. S190-S191
Author(s):  
T. Ferguson ◽  
D. Ward ◽  
S.J. Chantilis ◽  
K.L. Lee ◽  
D.M. Bookout ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Frozen Embryo Transfer ◽  
Success Rates ◽  
Patient Age ◽  
Patient Âgé ◽  
Blastocyst Quality

scholarly journals THE UTILITY OF PGT VS NON-PGT FROZEN EMBRYO TRANSFER BY PATIENT AGE GROUP

2020 ◽  
Vol 114 (3) ◽  
pp. e416-e417
Author(s):  
Christopher Hibray ◽  
Nicole George ◽  
Lynn B. Davis ◽  
Gerard Letterie ◽  
G. David Ball
Keyword(s):  
Embryo Transfer ◽  
Frozen Embryo Transfer ◽  
Age Group ◽  
Patient Age ◽  
Patient Âgé

P–237 The good, the fast and the slow: which is better? Live birth rate following single blastocyst frozen embryo transfer

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
P Sokol ◽  
E Clu. Obradó ◽  
M Sol Inarejos ◽  
M Parrieg. Beltrán ◽  
F Martíne. Sa. Andrés ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Embryo Transfer ◽  
Live Birth ◽  
Embryo Quality ◽  
Frozen Embryo Transfer ◽  
Birth Rates ◽  
Live Birth Rates ◽  
Actual Effect ◽  
Blastocyst Quality ◽  
The Impact

Abstract Study question Are embryo quality and day of vitrification (Day 5, 6 or 7) associated with live birth rates (LBR) following single blastocyst transfer (SBT) in frozen embryo transfer cycle (FET)? Summary answer Both blastocyst quality and day of vitrification are significantly associated with LBRs, with very low LBR when poor quality embryos are frozen on day 6. What is known already Evidence suggests that chromosomal status (ploidy) is strongly associated with blastocyst morphology and good quality embryos are more likely to be euploid. Furthermore, previous studies have shown a relationship between the time that embryos need to reach blastocyst stage and their euploidy rate with slowly developing blastocysts showing higher rate of aneuploidy. Nonetheless, despite all this evidence little is known about the actual effect of the combination of blastocyst quality and day of its vitrification. The scope of this study was to quantify the actual effect of the embryo quality and day of vitrification on live birth rates following FET. Study design, size, duration Retrospective analysis of 1546 FET cycles with SBT conducted between 2017 and 2019 in the university-affiliated private clinic. The embryos used for FET were obtained from IVF/ICSI: with PGT (FET-PGT) or without PGT (FET0) or from donated oocytes (FET-DON). Participants/materials, setting, methods FET with natural, natural-modified and completely medicated cycles to prepare endometrial lining were included. Blastocysts were classified according to Spanish Association for the Study of Reproductive Biology (ASEBIR) classification, ranging from A (the highest) to D (the lowest). The impact on LBR of different subgroups, formed within FET-PGT, FET0, FET-DON groups due to different day of vitrification and blastocyst quality, was assessed, using logistic regression after adjusting for age, day of vitrification and embryo quality. Main results and the role of chance We included 1546 FET cycles. Of those, 543 (35%) corresponded to FET-PGT; 648 (42%) to FET0 and 355 (23%) to FET-DON cycles. Overall, 1051 (68%) embryos were frozen on day 5(D5), 472 (30.5%) on day 6(D6) and 23 (1.5%) on day 7(D7). As far as embryo quality was concerned, 215 (13.9%) grade A; 957 (61.9%) B; 371(24%) C and 3(0.2%) D blastocysts were transferred. LBRs were significantly different between different embryos frozen on D5 44.3%; on D6 28.8% and on D7 8.7%, p < 0.001. When blastocyst quality was considered, LBR were 48.4% for grade A; 42.5% for B; 25.1% for C and 0% for D, p < 0.001. After applying logistic regression analysis, the odds ratio (OR) for transferring D6-blastocyst was 1.08, 95% CI[0.45; 2.62] and blastocyst with grade B and C; 0.71, 95% CI[0.51; 1.00]; 0.57,95% CI[0.36; 0.88] respectively. However, after transferring D6-blastocyst graded as C, the OR was 0.33, 95% CI[0.12; 0.90]. Our predictive model showed that the impact of the embryo quality on LBR was sustained across three groups. Transfer of D5/D6 grade A blastocyst resulted in the highest, while D6-C in the lowest LBR in all the groups. In the latter case vitrification on D6 impaired additionally the outcome. Limitations, reasons for caution The study should be interpreted with caution due to its retrospective character and the assessment of blastocyst quality on the day of vitrification and not on the day its transfer. Wider implications of the findings: Our robust findings could be considered a useful tool for counselling couples who seek advice regarding their expected success rates in the setting of FET with SBT. The very low livebirth rates in low quality (C) slow developing (D6) embryos should be communicated to patients prior to planning a FET. Trial registration number Not applicable

Reduction of multiple pregnancies and elimination of higher order multiple pregnancies by balancing blastocyst quality and patient age.

2001 ◽  
Vol 76 (3) ◽  
pp. S72
Author(s):  
M. Meintjes ◽  
J.A. Rodriguez ◽  
S.J. Chantilis ◽  
S.S. Crider-Pirkle ◽  
A.J. Rodriguez ◽  
...  
Keyword(s):  
Higher Order ◽  
Multiple Pregnancies ◽  
Patient Age ◽  
Patient Âgé ◽  
Blastocyst Quality

303 INDUCED MOTILITY OF MESA/TESE SPERMATOZOA IS ASSOCIATED WITH INCREASED FERTILIZATION AND IMPLANTATION RATES IN HUMAN

2006 ◽  
Vol 18 (2) ◽  
pp. 259 ◽  
Author(s):  
H. L. Zheng ◽  
L. J. Fengand
Keyword(s):  
Embryo Transfer ◽  
Sperm Motility ◽  
Testicular Sperm Extraction ◽  
Obstructive Azoospermia ◽  
Patient Age ◽  
Nutritional Elements ◽  
Group I ◽  
Patient Âgé ◽  
Implantation Rates ◽  
Group Ii

The key for human IVF using the intracytoplasmic sperm injection (ICSI) procedure is to find moving and living sperm in azoospermic men. The objective of this study was to determine the effect of induced sperm motility on the fertilization and implantation rates in humans. Testicular sperm extraction (TESE) medium containing sperm stimulators and nutritional elements is specially designed for male factor and/or poor quality sperm with lower motility. Men with obstructed or absent ejaculatory ducts were treated by using a microepididymal sperm aspiration (MESA) procedure. Sperm are aspirated directly from the epididymis for use in the ICSI procedure. Men with non-obstructive azoospermia were treated by using the TESE procedure. Small samples of testicular tissue are obtained by needle or microsurgical biopsy and a few sperm are painstakingly dissected out of the tissue for use in the ICSI procedure. Sperm specimens either from epididymis or testis were divided into the two respective groups. In Group I, the specimens were washed in a TESE medium plus 10% human serum albumin (HSA), and then incubated with TESE medium containing 10% HSA in a 5% CO2 incubator to induce sperm motility. In Group II, the specimens were washed in human tubal fluid (HTF) medium plus 10% HSA, and then incubated with HTF containing 10% HSA in a 5% CO2 incubator. After 1 h of incubation, the motility of spermatozoa was evaluated under a microscope. The ICSI procedure was performed under an inverted microscope. Fertilized eggs were cultured in HTF medium containing 10% serum substitute supplement (SSS) until Day 3 for embryo transfer. Patients' pregnancy was followed after embryo transfer. This study demonstrated that incubation of spermatozoa from MESA and TESE procedures in TESE medium, as compared with HTF medium, results in significantly increased sperm progression (MESA sperm: 2-3 vs. 0-1; TESE sperm: 1-1.5 vs. 0-0.1) and motility (MESA sperm: 12.0 � 1.8% vs. 3.3 � 0.5%; TESE sperm: 4.5 � 0.2% vs. 0.15 � 0.02%) all respectively. Induced motility of spermatozoa in TESE medium, as compared with HTF medium, is associated with a significantly increased fertilization rate (MESA sperm: 74.5 � 3.5% vs. 60.2 � 3.1; TESE sperm: 73 � 4.3% vs. 48.4 � 3.7%) and implantation rate (MESA sperm: 29.8 � 3.8% vs. 19.4 � 2.7%, for patient age 32 � 3.0, in Group I, n = 13, and in Group II, n = 12; TESE sperm: 23.2 � 3.3% vs. 15.4 � 2.8%, patient age 33 � 2.8%, in Group I, n = 11, and in Group II, n = 9), and significantly decreased the ICSI performing time (30 � 10 vs. 120 � 20 min), all respectively. TESE medium can significantly increase the motility of spermatozoa from TESE/MESA, because it contains specific sperm stimulators and nutritional elements that directly resulted in the increase of fertilization and implantation rates.

Does patient age impact physician embryo transfer rates within an in vitro fertilization program?

2004 ◽  
Vol 82 ◽  
pp. S209
Author(s):  
A. Eyvazzadeh ◽  
D. Ryley ◽  
A. Penzias ◽  
B.M. Berger ◽  
M. Alper ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Transfer ◽  
Fertilization Program ◽  
Patient Age ◽  
Vitro Fertilization ◽  
Transfer Rates ◽  
Patient Âgé

scholarly journals Success rates in isolating mesenchymal stem cells from permanent and deciduous teeth

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kengo Nakajima ◽  
Ryo Kunimatsu ◽  
Kazuyo Ando ◽  
Tomoka Hiraki ◽  
Kodai Rikitake ◽  
...  
Keyword(s):  
Stem Cells ◽  
Success Rate ◽  
Root Length ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
X Rays ◽  
Success Rates ◽  
Patient Age ◽  
Patient Âgé ◽  
Successful Isolation

AbstractStem cells from human exfoliated deciduous teeth (SHED) and human dental pulp stem cells (hDPSCs) have emerged as attractive cell sources for bone regeneration. However, the specific teeth and the conditions most suitable for stem cell isolation remain unclear. Therefore, the success rate of SHED and hDPSCs isolation, the patient age and remaining root length in deciduous teeth were evaluated. Successful isolation was defined as when the cell culture was maintained up to the third passage without any contamination or other issues. Remaining tooth length was calculated using the root-to-crown ratio from patient X-rays and compared to the norm value from the literature. The overall successful isolation rate of SHED and hDPSCs was 82% and 70%. The average patient ages at extraction of the deciduous teeth and permanent teeth were 11 years and 9 months, and 22 years and 10 months respectively. In the successful SHED group, the average remaining root length of the anterior deciduous teeth was 71.4%, and that of the deciduous molars was 61.4%. Successful isolation appears to be associated with patient age, length of the remaining root, and also mechanical stress and other factors. Tooth selection criteria need to be identified to improve the success rate.

P–378 Using a machine learning tool (72% accuracy with 64% PPV) to predict multiple live birth when transferring multiple embryos, based on embryo specific data

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
C A Pena ◽  
J Chambost ◽  
C Hickman ◽  
C Jacques ◽  
K Wiemer ◽  
...  
Keyword(s):  
Machine Learning ◽  
Embryo Transfer ◽  
Live Birth ◽  
Predictive Power ◽  
Cohort Analysis ◽  
Multiple Pregnancy ◽  
Patient Age ◽  
Patient Âgé ◽  
Increased Risk ◽  
Number Of Cells

Abstract Study question Can Machine Learning predict multiple pregnancy based on data specific to the embryos and the patient? Summary answer Embryo data are useful in determining which embryos are likely to lead to multiple pregnancy. Patient age has low predictive value compared to embryo data. What is known already Our previous assessment of the HFEA data demonstrated that single embryo transfer (SET) in the UK occurred in a minority (45%) of fresh cycles, with a marginal increase in live birth rate (LBR) in some patient cohorts in favor of multiple embryo transfer (MET). Current policies on determining number of embryos for transfer tend to be generic and do not account for detailed embryology data. Generic policies may compromise LBR for some patients that would benefit from MET. Artificial Intelligence has the potential to assist in this decision process. Study design, size, duration Retrospective cohort analysis from 2013 to 2020 of 193 cycles with 386 embryos used in double ETs on day 5 at POMA fertility clinic with positive live birth outcome. ML model, xgboost, was trained to predict multiple live birth (N = 54) versus single live birth (N = 139). Detailed embryology data from day 1 to day 5 were used as input. Participants/materials, setting, methods Input of the machine learning model included patient age and 18 morphological parameters collected on days 1, 2, 3 and 5 (symmetry, number of cells, blastocyst status, fragmentation, ICM and troph grades) from the two transferred embryos. An xgboost algorithm was trained on 80% of the data (n = 154) and tested on 20% of blind data (n = 39). Main results and the role of chance Xgboost machine learning algorithm predicted multiple live birth on the blind dataset with an accuracy of 72%, with an AUC of 0.60, showing better results than random. PPV (true prediction of multiple births) was 64% and NPV (true prediction of single birth) was 75%. The following parameters ranked high in the predictive power of the machine learning (in order of predictive power): blastocyst status on day 5 of both embryos, symmetry on day 3, number of cells on day 2, scores on day 2 and 3. Limitations, reasons for caution: The dataset was derived from a single clinic with manual annotations and may not be transferable to other clinics. The risk of bias is important as the model was trained only àon embryos that were transferred and led to at least one birth Wider implications of the findings: A tool to help identify which patients are at increased risk of MP with MET would be clinically useful to help patients and clinical team make the best personalised decision for a specific embryo, finding the balance between maximising success rate whilst minimising multiple pregnancy rate and its associated risks. Trial registration number Not applicable

Preoperative third ventricular bowing as a predictor of endoscopic third ventriculostomy success

2012 ◽  
Vol 9 (2) ◽  
pp. 182-190 ◽  
Author(s):  
Brian J. Dlouhy ◽  
Ana W. Capuano ◽  
Karthik Madhavan ◽  
James C. Torner ◽  
Jeremy D. W. Greenlee
Keyword(s):  
Endoscopic Third Ventriculostomy ◽  
Third Ventriculostomy ◽  
Lamina Terminalis ◽  
Time To Failure ◽  
Success Rates ◽  
Patient Age ◽  
The Third ◽  
Predominant Component ◽  
Patient Âgé ◽  
History Of

Object Patients with hydrocephalus often present with both intraventricular obstructive and communicating components, and determination of the predominant component is difficult. Other investigators have observed that third ventricular floor deformation, or “bowing” of the third ventricular floor, is a good indicator of intraventricular obstructive hydrocephalus, resulting in higher success rates with endoscopic third ventriculostomy (ETV). However, additional third ventricular bowing assessment and statistical evidence demonstrating a difference in ETV outcome with third ventricular bowing is needed. The authors hypothesized that patients with preoperative bowing of the third ventricle would exhibit greater long-term success rates after ETV and that lack of bowing would result in increased failure rates after ETV. Methods The authors determined success and failure for 59 ETVs performed in 56 patients, and recorded patient age, time to failure, and preoperative third ventricular anatomy, as well as history of infection, intraventricular hemorrhage, and previous shunt. Third ventricular anatomy was assessed on MR imaging for bowing, which was classified as any of the following: depression of the third ventricular floor, enlargement of the supraoptic recess, anterior curvature of the lamina terminalis, dilation of the proximal aqueduct to a greater extent than the distal aqueduct, and blunting or posterior bowing of the suprapineal recess. Univariate and multivariate analyses of ETV failure and the time to failure were performed using logistic regression and the Cox proportional hazards model, respectively. Results After adjusting for patient age and history of infection, there was a significant association between lack of anterior third ventricular preoperative bowing (either lamina terminalis, supraoptic recess, or third ventricular floor) and ETV failure (adjusted HR 2.79, 95% CI 1.08–7.20). Of the patients with bowing, 70.5% experienced success with ETV, as did 33.3% of the patients without bowing. Among the individual structures, absence of bowing in the anterior aspect of the third ventricular floor was significantly associated with censored time to ETV failure (multivariate HR 2.59, 95% CI 1.01–6.66; final model including age and history of infection). Conclusions The presence of preoperative third ventricular bowing is predictive of ETV success, with nearly a 3-fold likelihood of success compared with patients treated with ETV in the absence of such bowing. Although bowing is predictive, 33% of patients without bowing were also treated successfully with ETV.

Sign in / Sign up

Export Citation Format

Share Document