303 INDUCED MOTILITY OF MESA/TESE SPERMATOZOA IS ASSOCIATED WITH INCREASED FERTILIZATION AND IMPLANTATION RATES IN HUMAN

2006 ◽  
Vol 18 (2) ◽  
pp. 259 ◽  
Author(s):  
H. L. Zheng ◽  
L. J. Fengand
Keyword(s):  
Embryo Transfer ◽  
Sperm Motility ◽  
Testicular Sperm Extraction ◽  
Obstructive Azoospermia ◽  
Patient Age ◽  
Nutritional Elements ◽  
Group I ◽  
Patient Âgé ◽  
Implantation Rates ◽  
Group Ii

The key for human IVF using the intracytoplasmic sperm injection (ICSI) procedure is to find moving and living sperm in azoospermic men. The objective of this study was to determine the effect of induced sperm motility on the fertilization and implantation rates in humans. Testicular sperm extraction (TESE) medium containing sperm stimulators and nutritional elements is specially designed for male factor and/or poor quality sperm with lower motility. Men with obstructed or absent ejaculatory ducts were treated by using a microepididymal sperm aspiration (MESA) procedure. Sperm are aspirated directly from the epididymis for use in the ICSI procedure. Men with non-obstructive azoospermia were treated by using the TESE procedure. Small samples of testicular tissue are obtained by needle or microsurgical biopsy and a few sperm are painstakingly dissected out of the tissue for use in the ICSI procedure. Sperm specimens either from epididymis or testis were divided into the two respective groups. In Group I, the specimens were washed in a TESE medium plus 10% human serum albumin (HSA), and then incubated with TESE medium containing 10% HSA in a 5% CO2 incubator to induce sperm motility. In Group II, the specimens were washed in human tubal fluid (HTF) medium plus 10% HSA, and then incubated with HTF containing 10% HSA in a 5% CO2 incubator. After 1 h of incubation, the motility of spermatozoa was evaluated under a microscope. The ICSI procedure was performed under an inverted microscope. Fertilized eggs were cultured in HTF medium containing 10% serum substitute supplement (SSS) until Day 3 for embryo transfer. Patients' pregnancy was followed after embryo transfer. This study demonstrated that incubation of spermatozoa from MESA and TESE procedures in TESE medium, as compared with HTF medium, results in significantly increased sperm progression (MESA sperm: 2-3 vs. 0-1; TESE sperm: 1-1.5 vs. 0-0.1) and motility (MESA sperm: 12.0 � 1.8% vs. 3.3 � 0.5%; TESE sperm: 4.5 � 0.2% vs. 0.15 � 0.02%) all respectively. Induced motility of spermatozoa in TESE medium, as compared with HTF medium, is associated with a significantly increased fertilization rate (MESA sperm: 74.5 � 3.5% vs. 60.2 � 3.1; TESE sperm: 73 � 4.3% vs. 48.4 � 3.7%) and implantation rate (MESA sperm: 29.8 � 3.8% vs. 19.4 � 2.7%, for patient age 32 � 3.0, in Group I, n = 13, and in Group II, n = 12; TESE sperm: 23.2 � 3.3% vs. 15.4 � 2.8%, patient age 33 � 2.8%, in Group I, n = 11, and in Group II, n = 9), and significantly decreased the ICSI performing time (30 � 10 vs. 120 � 20 min), all respectively. TESE medium can significantly increase the motility of spermatozoa from TESE/MESA, because it contains specific sperm stimulators and nutritional elements that directly resulted in the increase of fertilization and implantation rates.

scholarly journals Comparison of implantation rates of euploid embryos based on day of biopsy and patient age

2018 ◽  
Vol 110 (4) ◽  
pp. e415
Author(s):  
C. Hibray ◽  
C.R. MacDonough ◽  
R. Halverson ◽  
N.A. Klein ◽  
G. Ball
Keyword(s):  
Patient Age ◽  
Patient Âgé ◽  
Implantation Rates

scholarly journals THE UTILITY OF PGT VS NON-PGT FROZEN EMBRYO TRANSFER BY PATIENT AGE GROUP

2020 ◽  
Vol 114 (3) ◽  
pp. e416-e417
Author(s):  
Christopher Hibray ◽  
Nicole George ◽  
Lynn B. Davis ◽  
Gerard Letterie ◽  
G. David Ball
Keyword(s):  
Embryo Transfer ◽  
Frozen Embryo Transfer ◽  
Age Group ◽  
Patient Age ◽  
Patient Âgé

scholarly journals Determination of the optimal concentration of Minitube Equex paste for boar semen cryopreservation based on sperm motility characteristics

2020 ◽  
Vol 52 (3) ◽  
Author(s):  
Junpen Suwimonteerabutr ◽  
Morakot Nuntapaitoon ◽  
Padet Tummaruk
Keyword(s):  
Sperm Motility ◽  
Egg Yolk ◽  
Group Iv ◽  
Optimal Concentration ◽  
Freezing And Thawing ◽  
Semen Cryopreservation ◽  
Group Iii ◽  
Group I ◽  
Boar Semen ◽  
Group Ii

Equex paste is a non-permeating cryoprotective agent (CPA) that improved post-thaw survival of spermatozoa during boar semen cryopreservation. However, Equex paste produced by Nova Chemical Sales Inc. (MA, USA) is not currently available. The aim of the present study was to determine the optimal concentration of Minitube Equex paste (Minitube, Tiefenbach, Germany) for boar semen cryopreservation in comparison with Nova Equex STM paste (control). Fifteen ejaculates from 12 mature boars were collected by the glove-hand method. Each ejaculate was aliquoted and cryopreserved in base freezing extender III as Tris-citrate egg yolk (TEY) extender plus 9.0% glycerol classified into four groups. Group I was the control and included only 1.5% Nova Equex STM paste. Groups II, III and IV were the experiment groups, and contained different concentrations of Minitube Equex paste (Group II: 1.5%; Group III: 1.7%; and Group IV: 1.9%) added to the freezing extender III. After freezing and thawing, sperm motility characteristics were evaluated by Sperm Class Analyzer® incubated at 37 °C for 0 (10 min), 1 and 2 h post-thawing. In Group IV after thawing at 0 h, rapid velocity and the velocity curved line were significantly higher than in Groups II and III (P < 0.05) but did not differ from Group I. Moreover, after thawing at 1 h, LIN (linearity) in Group IV was higher than in Group II (P < 0.05), but did not differ from the other groups. In conclusion, the most suitable concentration of Minitube Equex paste in the current protocol was 1.9% supplemented with 9.0% glycerol in TEY-based freezing extender III, based on the conformity between data from manual guides and the observed sperm motility characteristics results.

scholarly journals Analysis of Bull Sperm DNA Abnormalities Due to Cadmium Accumulation

2017 ◽  
Vol 6 (1) ◽  
pp. 5
Author(s):  
Fuad Fitriawan
Keyword(s):  
Sperm Motility ◽  
Dna Analysis ◽  
Cadmium Accumulation ◽  
Abnormal Sperm ◽  
Group I ◽  
Sperm Dna ◽  
Percentage Similarity ◽  
Bull Sperm ◽  
Group Ii ◽  
Percent Similarity

Sperm abnormalities can occur by various causes. Abnormality of sperm is usually characterized by abnormal sperm motility and viability. This was caused by the inability of mitochondria on ATP-ase in producing ATP and ecto-enzyme Cik role in keeping the movement so that movement of sperm motility declines. Research that leads to total abnormal sperm DNA analysis is still rare. The purpose of this research was to get the results of the molecular characteristic picture of the overall characteristics of DNA loci that have abnormalities in bull sperm and get a picture of differences in overall DNA loci of abnormal and normal sperm. This research was conducted in October-December 2016. With the results of Group I consists of D, E with a percent similarity of 92.308%, group II consists of C and Group I with a percentage similarity of 50.125%, group II consists of A and B with a percent similarity of 100%, group II and group III with a percentage similarity of 0%. Based on the above data it can be concluded that the treatment A and B is not suspected to cause DNA damage compared to treatment C, D and E.

scholarly journals comparison between Cleavage Stage versus Blastocyst Stage Embryo Transfer in an Egyptian Cohort Undergoing in vitro Fertilization: A possible Role for Laser Assisted Hatching

2011 ◽  
Vol 5 ◽  
pp. CMRH.S7735 ◽  
Author(s):  
Sherif F. Hendawy ◽  
TA Raafat
Keyword(s):  
Embryo Transfer ◽  
Intracytoplasmic Sperm Injection ◽  
Blastocyst Transfer ◽  
Assisted Hatching ◽  
Vitro Fertilization ◽  
Significant Difference ◽  
Implantation Rates ◽  
Group Ii ◽  
In Pregnancy

Background Extended in vitro embryo culture and blastocyst transfer have emerged as essential components of the advanced reproductive technology armamentarium, permitting selection of more advanced embryos considered best suited for transfer. Aim of study The aim of this study was to compare between cleavage stage and blastocyst stage embryo transfer in patients undergoing intracytoplasmic sperm injection, and to assess the role of assisted hatching technique in patients undergoing blastocyst transfer. Patients and methods This study was carried out on two groups. Group I: 110 patients who underwent 120 cycles of intracytoplasmic sperm injection with day 2-3 embryo transfer—for unexplained infertility or male factor within the previous 3 years. Their data obtained retrospectively from medical records. Group II: 46 age matched infertile female patients undergoing 51 intracytoplasmic sperm injection cycles for similar causes. Patients in Group II were further subdivided into 2 equal subgroups; Group Ila (23 patients), which had laser assisted hatching and Group IIb (23 patients), which did not have assisted hatching. All patients had an infertility workup including basal hormonal profile, pelvic ultrasound, hysterosalpingogram and/or laparoscope and semen analysis of the patient's partner. All patients underwent controlled ovarian hyperstimulation: Using long protocol of ovulation induction. Laser assisted hatching was done for blastocysts of 23 patients. Results Comparison between both groups as regards the reproductive outcome showed a significant difference in pregnancy and implantation rates, both being higher in group II ( P < 0.05) Comparison between both subgroups as regards the reproductive outcome showed a highly significant difference in pregnancy and implantation rates, both being higher in Group IIa ( P < 0.01). There was also a significantly higher rate of multiple pregnancies among Group IIa ( P < 0.05). Conclusion Blastocyst transfer is a successful and improved alternative for patients with multiple failed in vitro fertilization attempts, associated with a significant increase in pregnancy and implantation rates. Furthermore, laser assisted hatching increases implantation and clinical pregnancy rates.

scholarly journals Comparison Between Lignocaine Hydrochloride and Ropivacaine Hydrochloride as Lumbosacral Epidural Anaesthetic Agents in Goats Undergoing Laparoscopy Assisted Embryo Transfer

2014 ◽  
Vol 37 (2) ◽  
pp. 141-149 ◽  
Author(s):  
Anubhav Khajuria ◽  
Mujeeb ur Rehman Fazili ◽  
Riaz Ahmad Shah ◽  
Maajid Hassan Bhat ◽  
Firdous Ahmad Khan ◽  
...  
Keyword(s):  
Motor Activity ◽  
Embryo Transfer ◽  
Regional Anaesthesia ◽  
Anaesthetic Agents ◽  
Group I ◽  
Baseline Values ◽  
Group Ii ◽  
Epidural Anaesthetic ◽  
Ropivacaine Hydrochloride ◽  
Surgical Manipulations

AbstractGoats (n=12) undergoing laparoscopy assisted embryo transfer were randomly allotted to two groups (I and II) and injected lignocaine hydrochloride (4mg/kg) or ropivacaine hydrochloride (1mg/kg) at the lumbosacral epidural space. The animals were held with raised hind quarters for the first three minutes following injection. Immediately after induction of regional anaesthesia, they were restrained in dorsal recumbency in the Trendelenburg position in a cradle. Laparoscopy was performed after creating pneumoperitoneum using filtered room air. The mean (± S.E) induction time in animals of group I was significantly shorter (5.33 ± 0.61 min) than those belonging to group II (12.66 ±1.99 min). Complete analgesia developed throughout the hind quarters and abdomen for 30 min and 60 min in group I and II animal’s respectively. Unlike animals of group I, group II goats continued to show moderate analgesia for 180 minutes. The motor activity returned after a lapse of 130.00 ± 12.64 min and 405.00 ± 46.31 min respectively. Occasional vocalization and struggling was noticed in two goats one from each group irrespective of the surgical manipulations during laparoscopy. The rectal temperature and respiration rates showed only non-significant increase, but the heart rate values were significantly higher (P < 0.5) up to 150 min in animals of both the groups when compared to their baseline values. From this study, it was concluded that both anaesthetic agents produced satisfactory regional anaesthesia in goats undergoing laparoscopy. However, considering the very long delay in regaining the hind limb motor activity, the use of ropivacaine may not be recommended for this purpose. Supplementation of sedative/tranquilizer with lumbosacral epidural anaesthesia needs evaluation.

26 SPERM MOTILITY DECREASING AND SEMEN FERTILITY IN THE BULL EVALUATED BY BIOSPECKLE

2010 ◽  
Vol 22 (1) ◽  
pp. 170 ◽  
Author(s):  
R. S. Macedo ◽  
J. B. Barreto Filho ◽  
R. A. Braga Jr ◽  
G. F. Rabelo
Keyword(s):  
Sperm Motility ◽  
Laser Light ◽  
Bos Taurus ◽  
Test Group ◽  
Mean Value ◽  
Ultrasound Images ◽  
Group I ◽  
Conception Rates ◽  
Inertial Moment ◽  
Group Ii

Sperm motility decreasing in a semen sample over time is an indirect approach to assess spermatozoa viability and should be related to the ejaculate fertility. The biospeckle (BSL) is an interacting phenomenon between laser light and biological specimens that allows measurement of sperm kinetic activity by means of an index called the inertial moment (IM). The aim of this work was to evaluate sperm motility diminishing in frozen bovine semen with BSL and analyze semen fertility in relation to the motility decreasing behavior patterns showed by different semen samples. Fertility was assessed by beef heifer conception rates after AI. Semen of 6 mature IA donor bulls (Bos taurus indicus) was previously divided in 2 groups (group I: motility ≥50%; group II: motility <50%), each group comprised 3 animals. Semen was thawed at 37°C for 30 sin a water bath. One aliquot of 10 μL was placed in a warmed slide, covered with a slip, and evaluated by light microscopy and BSL just after thawing. Then, samples were kept at room temperature to induce a decrease in sperm motility. Each sample was illuminated 6 times by laser light at intervals of 2 min (n = 36) and IM values were obtained. The semen samples were used in an AI program of beef heifers (n = 166) under the same nutrition and management conditions. All inseminations were done by the same technician, and donor bulls were used consecutively throughout the breeding season. Pregnancy diagnosis was done by ultrasound images (Falco 100, 6 MHz, Pie Medical, Crawley, UK) 28 days after insemination. The SISVAR software was used in variance analysis and comparison of means by the Tukey test. Group I showed greater (P < 0.05) IM mean value (153.5 ± 27.48) compared with group II (107.94 ± 30.61), which means that this group had higher sperm motility during the time of evaluation. In addition, conception rates (0.63 ± 0.49) for group I were also higher (P < 0.05) compared with group II. Apparently semen fertility is related to the sperm kinetics measured by the IM. Mean IM values (134.0 ± 22.65 and 96 ± 22.34, groups I and II, respectively) obtained in the last illumination for each group did not differ (P > 0.05), but a tendency to differ was observed when a greater number of illuminations was done. Our data suggest that the BSL was capable of identifying spermatozoa surviving between high and low motility groups, and the sperm motility decreasing measured by the IM might be an objective approach to evaluate semen fertilization potential. Financial support: FAPEMIG, grant EDT 94/07, CNPq.

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