Natural imidazole alkaloids as antibacterial agents against Pseudomonas syringae pv. actinidiae isolated from kiwi endophytic fungus Fusarium tricinctum

The volatile oil, obtained by hydrodistillation from Fusarium tricinctum, the endophytic fungus isolated from Paris polyphylla var. yunnanensis, was analyzed by gas chromatography-mass spectrometry (GC-MS). trans-1,2,3,3a,4,7a-Hexahydro-7a-methyl-5H-inden-5-one (73.1%), 2-methylene-4,8,8-trimethyl-4-vinyl bicyclo [5.2.0] nonane (12.0%), and 2,6-dimethyl-6-(4-methyl-3-pentenyl) bicyclo [3.1.1] hept-2-ene (4.5%) were the major compounds of the 15 identified components accounting for 95.4% of the volatile oil. The antimicrobial activity of the volatile oil was assayed against eight bacteria and two fungi. The minimum inhibitory concentration (MIC) values of the volatile oil against the test bacteria ranged from 25 to 45 μg/mL. The MIC values against the fungi Candida albicans and Magnaporthe oryzae were 100 and 225 μg/mL, respectively. The mean inhibitory concentration (IC50) values of the volatile oil against the test bacteria ranged from 17.8 to 31.6 μg/mL, and those of the volatile oil against C. albicans and M. oryzae were 84.3 and 204.3 μg/mL, respectively.

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Cycloaspeptide A and Pseurotin A from the Endophytic Fungus Penicillium janczewskii

Zeitschrift für Naturforschung C ◽

10.1515/znc-2008-5-612 ◽

2008 ◽

Vol 63 (5-6) ◽

pp. 383-388 ◽

Cited By ~ 27

Author(s):

Guillermo Schmeda-Hirschmann ◽

Emilio Hormazabal ◽

Jaime A. Rodriguez ◽

Cristina Theoduloz

Keyword(s):

Pseudomonas Syringae ◽

Endophytic Fungus ◽

Cyclic Peptides ◽

Human Lung ◽

Erwinia Carotovora ◽

Lung Fibroblasts ◽

Spectroscopic Methods ◽

Moderate Effect ◽

Low Cytotoxicity ◽

First Time

Penicillium janczewskiiK. M. Zalessky was isolated as an endophytic fungus from the phloem of the Chilean gymnosperm Prumnopitys andina. When grown in liquid yeast extractmalt extract-glucose broth, the fungus produced two main secondary metabolites. The compounds were for the first time isolated from this species and identified by spectroscopic methods as pseurotin A and cycloaspeptide A. This is the first report on the production of cyclic peptides by endophytic fungi from Chilean gymnosperms. Pseurotin A and cycloaspeptide A presented low cytotoxicity towards human lung fibroblasts with IC50 ≥ 1000 μm. Pseurotin A showed a moderate effect against the phytopathogenic bacteria Erwinia carotovora and Pseudomonas syringae, with IC50 values of 220 and 112 μg ml-1, respectively.

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Fusarium tricinctum, An Endophytic Fungus Exhibits Cell Growth Inhibition and Antioxidant Activity

Indian Journal of Microbiology ◽

10.1007/s12088-016-0600-x ◽

2016 ◽

Vol 56 (4) ◽

pp. 433-438 ◽

Cited By ~ 9

Author(s):

M. Vasundhara ◽

Manoj Baranwal ◽

Anil Kumar

Keyword(s):

Antioxidant Activity ◽

Cell Growth ◽

Growth Inhibition ◽

Endophytic Fungus ◽

Cell Growth Inhibition ◽

Fusarium Tricinctum

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Zopfiellasins A–D, Two Pairs of Epimeric Cytochalasins from Kiwi-Associated Fungus Zopfiella sp. and Their Antibacterial Assessment

Molecules ◽

10.3390/molecules26185611 ◽

2021 ◽

Vol 26 (18) ◽

pp. 5611

Author(s):

Jie-Yu Zhang ◽

Juan He ◽

Zheng-Hui Li ◽

Tao Feng ◽

Ji-Kai Liu

Keyword(s):

Antibacterial Activity ◽

Data Analysis ◽

Single Crystal ◽

Pseudomonas Syringae ◽

Spectroscopic Data ◽

Antibacterial Agents ◽

X Ray Diffraction ◽

X Ray ◽

First Report ◽

The Absolute

In our continuous search for antibacterial agents against Pseudomonas syringae pv. actinidiae (Psa) from kiwi-associated fungi, two pairs of epimeric cytochalasins, zopfiellasins A–D (1–4), were characterized from the fungus Zopfiella sp. The structures were established on the basis of spectroscopic data analysis, while the absolute configurations were determined by single-crystal X-ray diffraction. Compounds 1 and 3 exhibited antibacterial activity against Psa with MIC values of 25 and 50 μg/mL, respectively. This is the first report of anti-Psa activity of cytochalasin derivatives.

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