Application of essential oils in maize grain: Impact on Aspergillus section Flavi growth parameters and aflatoxin accumulation

2008 ◽  
Vol 25 (2) ◽  
pp. 324-334 ◽  
Author(s):  
Romina V. Bluma ◽  
Miriam G. Etcheverry
Keyword(s):  
Essential Oils ◽  
Growth Parameters ◽  
Aspergillus Section Flavi ◽  
Aflatoxin Accumulation ◽  
Maize Grain ◽  
Aspergillus Section

Natural maize phytochemicals for control of maize mycoflora and aflatoxigenic fungi

2009 ◽  
Vol 2 (3) ◽  
pp. 305-312 ◽  
Author(s):  
A. Nesci ◽  
S. Marín ◽  
M. Etcheverry ◽  
V. Sanchis
Keyword(s):  
Ferulic Acid ◽  
Incubation Period ◽  
Cinnamic Acid ◽  
Inhibitory Effect ◽  
Aspergillus Section Flavi ◽  
Aflatoxigenic Fungi ◽  
Incubation Periods ◽  
Maize Grain ◽  
Aflatoxin B ◽  
Aspergillus Section

This research was undertaken to evaluate the effects of the natural phytochemicals trans-cinnamic acid (CA) alone at concentrations of 20 and 25 mM, ferulic acid (FA) at concentration of 30 mM and two mixtures, CA-FA (20+30 mM) and CA-FA (25+30 mM) on natural maize mycoflora, Aspergillus section Flavi population and aflatoxin B1 production. These studies were carried out in maize grain in relation to a water activity of 0.99, 0.97 and 0.94. CA at 25 mM and the mixture CA-FA (25+30 mM) were the most effective treatments at inhibiting natural maize mycoflora at all aw assayed after 11 and 35 days of incubation at 25 °C. In general, 20 mM CA caused complete inhibition of Aspergillus section Flavi population at all aw values tested during all incubation period without an additional inoculum. 20 mM CA and 25 mM CA showed the major inhibitory effect on aflatoxin B1 accumulation of control and Aspergillus section Flavi additionally inoculated during all incubation periods. The data showed that CA and FA could be considered as effective fungitoxicants for natural maize mycoflora and aflatoxigenic fungi in the aw range 0.99 to 0.94. The information obtained shows promise for controlling aflatoxigenic fungi in stored maize.

SCREENING OF PLANT EXTRACTS ON GROWTH PARAMETERS OF TWO ASPERGILLUS SECTION FLAVI TOXIGENIC STRAINS

2021 ◽  
pp. 44-56
Author(s):  
Mariana Paiva Rodrigues ◽  
Tatiana Xavier de Almeida ◽  
Águida Aparecida de Oliveira ◽  
Gabriela Lago Biscoto ◽  
Raul Roque de Souza Dias ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Growth Parameters ◽  
Aspergillus Section Flavi ◽  
Toxigenic Strains ◽  
Aspergillus Section

scholarly journals Influence of a glyphosate-based herbicide on growth parameters and aflatoxin B1 production by Aspergillus section Flavi on maize grains

2020 ◽  
Author(s):  
Nicolás Benito ◽  
Karen Magnoli ◽  
Cecilia Soledad Carranza ◽  
Melisa Eglé Aluffi ◽  
Carina Elizabeth Magnoli ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Growth Parameters ◽  
Aspergillus Section Flavi ◽  
Aspergillus Section

Food-grade antioxidants for control of Aspergillus section Flavi and interrelated mycoflora of stored peanuts with different water activities

2009 ◽  
Vol 2 (4) ◽  
pp. 399-407 ◽  
Author(s):  
M. Passone ◽  
M. Doprado ◽  
M. Etcheverry
Keyword(s):  
Inhibitory Effect ◽  
Butylated Hydroxytoluene ◽  
Butylated Hydroxyanisole ◽  
Food Grade ◽  
Metabolite Production ◽  
Aspergillus Section Flavi ◽  
Fungal Populations ◽  
Aflatoxin Accumulation ◽  
Aspergillus Section

The inhibitory effect of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) mixture on fungal populations, Aspergillus section Flavi and aflatoxins accumulation in in-pod peanuts during storage in big bags was investigated. In-pod peanuts were previously conditioned at different water activities (0.94, 0.88, 0.84 and 0.76 aw) and treated with food-grade antioxidants. Both control and treated peanuts were stored for 6 months and sampled at monthly intervals. BHA-BHT mixture reduced the incidence of peanut fungal populations between 0.6-20.4% and between 1.2-33.1% during 1-3 and 4-5 storage months, respectively. Aspergillus section Flavi counts decreased with 36.5%, 46.3% and 77.4% in peanuts conditioned at 0.94, 0.84 and 0.76 aw levels and treated with antioxidants. At the above peanut aw conditions, the treatment applied reduced aflatoxin accumulation by 72.1%, while any effect on this metabolite production was observed at 0.88 aw. The antioxidant formulation used in this study has the potential to control aflatoxigenic populations in in-pod peanuts stored in half-permeable silos at ≤0.84 aw level.

scholarly journals Oil and Flower Production in Rosa damascena trigintipetala Dieck under Salinity Stress in Taif Region, Saudi Arabia

2021 ◽  
Vol 13 (8) ◽  
pp. 4547
Author(s):  
Mohamed E. El-Sharnouby ◽  
Metwally M. Montaser ◽  
Sliai M. Abdallah
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Salinity Stress ◽  
Growth Parameters ◽  
Industrial Applications ◽  
Rosa Damascena ◽  
Flower Production ◽  
Survival Percentage ◽  
The Impact ◽  
Tolerance To Salinity

The flower industry depends on oil and fragrance, which is addressed in the current work. Different concentrations of NaCl (0, 250, 500, 1000, and 1500 ppm) were applied to Taif rose plants (Rosa damascena var. trigintipetala Dieck) to evaluate their effects on growth and essential oil content. Results clearly indicated the highest survival percentage (98.3%) was seen in untreated plants compared to plants under salinity stress. Moreover, increasing the NaCl levels induced an adverse effect on the growth parameters of Taif rose plants, while some essential oil contents were increased to the maximum degree of their tolerance to salinity stress. The extracted essential oils were analyzed using GC/MS. The essential oils of Taif rose plants treated with 500 ppm NaCl recorded the highest values of citronellol, geraniol and phenylethyl alcohol contents (16.56, 8.67 and 9.87%), respectively. NaCl at 250 ppm produced the highest values of heneicosane (13.12%), and then decreased to the lowest value (7.79%) with the increase of NaCl to 1500 NaCl, compared to the control and other NaCl levels. The current results could highlight the impact of salinity stress on Rosa damascena Miller var. trigintipetala Dieck for better economic and industrial applications.

Prevalence of Aspergillus species on selected Colombian animal feedstuffs and ability of Aspergillus section Flavi to produce aflatoxins

2009 ◽  
Vol 2 (1) ◽  
pp. 31-34 ◽  
Author(s):  
G. Diaz ◽  
M. Lozano ◽  
A. Acuña
Keyword(s):  
Liquid Chromatography ◽  
Cottonseed Meal ◽  
Sunflower Seed ◽  
Seed Meal ◽  
Aspergillus Section Flavi ◽  
Wheat Middlings ◽  
Highperformance Liquid Chromatography ◽  
Animal Feedstuffs ◽  
Aflatoxin B ◽  
Aspergillus Section

A total of 57 samples of feedstuffs commonly used for animal nutrition in Colombia (maize, soybean, sorghum, cottonseed meal, sunflower seed meal, wheat middlings and rice) were analysed for Aspergillus contamination. Aspergillus fungi were identified at species level and their ability to produce aflatoxins was determined by highperformance liquid chromatography. A total of 31 of the feedstuffs analysed (54.4%) were found to contain Aspergillus spp. The most contaminated substrate was maize (100%) followed by cottonseed meal (80%), sorghum (60%) and wheat middlings (60%). Soybean showed lower levels of contamination (10%). No Aspergillus spp. could be isolated from rice or sunflower seed meal. Total Aspergillus strains isolated were 50, with 28 belonging to section Flavi (56%), 17 to section Nigri (34%), 4 to section Circumdati (8%) and 1 to section Fumigati (2%). Among section Flavi, 17 isolates were identified as A. flavus, seven as A. parasiticus, two as A. oryzae and two as A. tamarii. Production of aflatoxins by Aspergillus section Flavi was screened by liquid chromatography. About three quarters of the A. flavus strains (76.5%) produced aflatoxin B1 (0.2 to 240.4 µg/g) and aflatoxin B2 (0.2 to 1.6 µg/g), while all A. parasiticus strains produced the four naturally occurring aflatoxins (aflatoxin B1 from 0.6 to 83.5 µg/g, aflatoxin B2 from 0.3 to 4.8 µg/g, aflatoxin G1 from 0.4 to 19.3 µg/g and aflatoxin G2 from 0.1 to 1.0 µg/g). This is the first study demonstrating the presence of highly toxigenic Aspergillus fungi in Colombian animal feedstuffs.

Modelling the colonisation of maize by toxigenic and non-toxigenic Aspergillus flavus strains: implications for biological control

2008 ◽  
Vol 1 (3) ◽  
pp. 333-340 ◽  
Author(s):  
H. Abbas ◽  
R. Zablotowicz ◽  
H. Bruns
Keyword(s):  
Biological Control ◽  
Aspergillus Flavus ◽  
Growth Parameters ◽  
Biological Control Agent ◽  
Cyclopiazonic Acid ◽  
Aflatoxin Contamination ◽  
Tween 20 ◽  
Lag Phase ◽  
Gompertz Equation ◽  
Aflatoxin Accumulation

To successfully exploit biological control it is desirable to understand how the introduced agent colonises the host and interferes with establishment of the pest. This study assessed field colonisation of maize by Aspergillus flavus strains as biological control agents to reduce aflatoxin contamination. Maize (corn, Zea mays L.) ears were inoculated with A. flavus using a pin-bar technique in 2004 and 2005. Non-aflatoxigenic strains K49 (NRRL 30797) & CT3 (NRRL 30798) and toxigenic F3W4 (NRRL 30798) were compared against a carrier control (0.2% aqueous Tween 20). Ten ears were sampled over 12 to 20 days, visually assessed, and curves fit to a three compartment Gompertz equation or other best appropriate regressions. Aflatoxin was determined by HPLC and cyclopiazonic acid (CPA) by LC/MS. The Gompertz model describes growth parameters, e.g. growth constant, lag phase and maximum colonisation characterised patterns of maize colonisation for most inoculated treatments. Aflatoxin accumulation in maize inoculated with F3W4 was about 35,000 ng/g in 2004 and 2005, with kinetics of aflatoxin accumulation in 2005 well described by the Gompertz equation. Less than 200 ng/g was observed in maize inoculated with strains CT3 & K49 and accumulation was described by a linear or logistic model. Maize inoculated with strains CT3 and F3W4 accumulated a maximum of 220 and 169 µg/kg CPA, respectively, compared to 22 and 0.2 µg/kg in the control and K49 inoculated, respectively. This technique can be used to elucidate colonisation potential of non-toxigenic A. flavus in maize in relation to biological control of aflatoxin. The greatest reduction of aflatoxin and CPA in maize inoculated with strain K49 and Gompertz parameters on colonisation indicates its superiority to CT3 as a biological control agent. The dynamics of maize colonisation by A. flavus strains and subsequent mycotoxin accumulation generated by using the pin-bar technique has implications for characterising the competence of biocontrol strains for reducing aflatoxin contamination.

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