Digestion of micellar casein in duodenum cannulated pigs. Correlation between in vitro simulated gastric digestion and in vivo data

Gastric emptying of food is mainly driven by the caloric concentration, the rheological properties of the chyme, and the physical state (liquid/solid) of food once in the stomach. The present work investigated: (1) The effect of the composition and the viscosity of drinkable yogurts on gastric emptying in pigs, and (2) the behavior of yogurts during dynamic in vitro digestion. Three isocaloric liquid yogurts were manufactured: Two enriched in protein and fiber showing either a low (LV) or high (HV) viscosity, one control enriched in sugar and starch (CT). They were labelled with 99mTc-sulfur colloid and given to pigs (n = 11) to determine gastric emptying pattern by gamma scintigraphy. Then dynamic in vitro digestion of the yogurts was done using the parameters of gastric emptying determined in vivo. Gastric emptying half-times were significantly longer for LV than CT, whereas HV exhibited an intermediate behavior. In vitro gastric digestion showed a quick hydrolysis of caseins, whereas whey proteins were more resistant in the stomach particularly for LV and HV. During the intestinal phase, both whey proteins and caseins were almost fully hydrolyzed. Viscosity was shown to affect the behavior of yogurt in the small intestine.

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The absorption, distribution, metabolism and excretion of procyanidins

Food & Function ◽

10.1039/c5fo01244a ◽

2016 ◽

Vol 7 (3) ◽

pp. 1273-1281 ◽

Cited By ~ 64

Author(s):

Liang Zhang ◽

Yijun Wang ◽

Daxiang Li ◽

Chi-Tang Ho ◽

Junsong Li ◽

...

Keyword(s):

Small Intestine ◽

Comparative Analysis ◽

Published Data ◽

Gastric Digestion ◽

Colonic Microflora

This review summarizes research on thein vivoandin vitroADME of procyanidins (PAs), including the degradation of PAs in gastric digestion, their absorption in the small intestine and the pathway of their metabolism by colonic microflora. A systematic comparative analysis of published data is performed to elucidate the pharmacokinetics of PAsin vivo.

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Physical Property Changes in Raw and Roasted Almonds during Gastric Digestion In vivo and In vitro

Food Biophysics ◽

10.1007/s11483-013-9315-2 ◽

2013 ◽

Vol 9 (1) ◽

pp. 39-48 ◽

Cited By ~ 19

Author(s):

Gail M. Bornhorst ◽

Maxine J. Roman ◽

Krista C. Dreschler ◽

R. Paul Singh

Keyword(s):

Physical Property ◽

Gastric Digestion ◽

Property Changes

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Structural breakdown of starch‐based foods during gastric digestion and its link to glycemic response: In vivo and in vitro considerations

Comprehensive Reviews in Food Science and Food Safety ◽

10.1111/1541-4337.12749 ◽

2021 ◽

Author(s):

Joanna Nadia ◽

John Bronlund ◽

Rajinder Paul Singh ◽

Harjinder Singh ◽

Gail M. Bornhorst

Keyword(s):

Glycemic Response ◽

Gastric Digestion ◽

Structural Breakdown

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Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053425 ◽

1982 ◽

Vol 40 ◽

pp. 142-145

Author(s):

E. J. Kollar

Keyword(s):

Connective Tissue ◽

Oral Mucosa ◽

Basal Lamina ◽

Functional Derivatives ◽

Specific Source ◽

Dental Epithelium ◽

Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

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Ultrastructural Correlations between Clonal Human Tumor Colonies and in Vivo Neoplasms

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053711 ◽

1982 ◽

Vol 40 ◽

pp. 210-211

Author(s):

M.J. Murphy ◽

R.R. Price ◽

J.C. Sloman

Keyword(s):

Cultured Cells ◽

Human Tumor ◽

Cell Type ◽

Tumor Mass ◽

Initial Cell ◽

Cloning Assay ◽

Human Tumor Cloning ◽

The Relationship

The in vitro human tumor cloning assay originally described by Salmon and Hamburger has been applied recently to the investigation of differential anti-tumor drug sensitivities over a broad range of human neoplasms. A major problem in the acceptance of this technique has been the question of the relationship between the cultured cells and the original patient tumor, i.e., whether the colonies that develop derive from the neoplasm or from some other cell type within the initial cell population. A study of the ultrastructural morphology of the cultured cells vs. patient tumor has therefore been undertaken to resolve this question. Direct correlation was assured by division of a common tumor mass at surgical resection, one biopsy being fixed for TEM studies, the second being rapidly transported to the laboratory for culture.

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