Gastric Digestion In Vivo and In Vitro: How the Structural Aspects of Food Influence the Digestion Process

2014 ◽  
Vol 5 (1) ◽  
pp. 111-132 ◽  
Author(s):  
Gail M. Bornhorst ◽  
R. Paul Singh
Keyword(s):  
Gastric Digestion ◽  
Digestion Process ◽  
Structural Aspects

Conjugates of Classical DNA/RNA Binder with Nucleobase: Chemical, Biochemical and Biomedical Applications

2019 ◽  
Vol 26 (30) ◽  
pp. 5609-5624
Author(s):  
Dijana Saftić ◽  
Željka Ban ◽  
Josipa Matić ◽  
Lidija-Marija Tumirv ◽  
Ivo Piantanida
Keyword(s):  
Molecular Weight ◽  
Small Molecules ◽  
Biomedical Applications ◽  
Protein Targets ◽  
New Class ◽  
Rna Targets ◽  
Covalently Linked ◽  
Structural Aspects

: Among the most intensively studied classes of small molecules (molecular weight < 650) in biomedical research are small molecules that non-covalently bind to DNA/RNA, and another intensively studied class is nucleobase derivatives. Both classes have been intensively elaborated in many books and reviews. However, conjugates consisting of DNA/RNA binder covalently linked to nucleobase are much less studied and have not been reviewed in the last two decades. Therefore, this review summarized reports on the design of classical DNA/RNA binder – nucleobase conjugates, as well as data about their interactions with various DNA or RNA targets, and even in some cases protein targets are involved. According to these data, the most important structural aspects of selective or even specific recognition between small molecule and target are proposed, and where possible related biochemical and biomedical aspects were discussed. The general conclusion is that this, rather new class of molecules showed an amazing set of recognition tools for numerous DNA or RNA targets in the last two decades, as well as few intriguing in vitro and in vivo selectivities. Several lead research lines show promising advancements toward either novel, highly selective markers or bioactive, potentially druggable molecules.

Compositional, Structural, and Kinetic Aspects of Lipid Digestion and Bioavailability: In Vitro, In Vivo, and Modeling Approaches

2022 ◽  
Vol 13 (1) ◽  
Author(s):  
Sébastien Marze
Keyword(s):  
Annual Review ◽  
Publication Date ◽  
Lipid Digestion ◽  
Modeling Approaches ◽  
Digestion Kinetics ◽  
Food Design ◽  
Structural Aspects ◽  
Development And Evolution

Lipid digestion and bioavailability are usually investigated separately, using different approaches (in vitro, modeling, in vivo). However, a few inclusive studies show that their kinetics are closely linked. Lipid bioavailability kinetics is likely involved in the development and evolution of several diseases, so lipid digestion kinetics could be involved as well and can be modulated by food design or combination. To illustrate this possibility, the compositional and structural aspects of lipid digestion kinetics, as investigated using in vitro and modeling approaches, are presented first. Then, in vivo and mixed approaches enabling the study of both kinetics are reviewed and discussed. Finally, disparate modeling approaches are introduced, and a unifying modeling scheme is proposed, opening new perspectives for understanding the role and interactions of various factors (chemical, physical, and biological) involved in lipid metabolism. Expected final online publication date for the Annual Review of Food Science and Technology, Volume 13 is March 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

Digestion of micellar casein in duodenum cannulated pigs. Correlation between in vitro simulated gastric digestion and in vivo data

2021 ◽  
Vol 343 ◽  
pp. 128424 ◽  
Author(s):  
B. Miralles ◽  
J. Sanchón ◽  
L. Sánchez-Rivera ◽  
D. Martínez-Maqueda ◽  
Y. Le Gouar ◽  
...  
Keyword(s):  
Gastric Digestion ◽  
Micellar Casein

Impact of food components during in vitro digestion of silver nanoparticles on cellular uptake and cytotoxicity in intestinal cells

2015 ◽  
Vol 396 (11) ◽  
pp. 1255-1264 ◽  
Author(s):  
Dajana Lichtenstein ◽  
Johanna Ebmeyer ◽  
Patrick Knappe ◽  
Sabine Juling ◽  
Linda Böhmert ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cellular Uptake ◽  
In Vitro Digestion ◽  
Intestinal Cells ◽  
Digestion Process ◽  
X Ray Scattering ◽  
Food Components ◽  
Uptake Studies

Abstract Because of the rising application of nanoparticles in food and food-related products, we investigated the influence of the digestion process on the toxicity and cellular uptake of silver nanoparticles for intestinal cells. The main food components – carbohydrates, proteins and fatty acids – were implemented in an in vitro digestion process to simulate realistic conditions. Digested and undigested silver nanoparticle suspensions were used for uptake studies in the well-established Caco-2 model. Small-angle X-ray scattering was used to estimate particle core size, size distribution and stability in cell culture medium. Particles proved to be stable and showed radii from 3.6 to 16.0 nm. Undigested particles and particles digested in the presence of food components were comparably taken up by Caco-2 cells, whereas the uptake of particles digested without food components was decreased by 60%. Overall, these findings suggest that in vivo ingested poly (acrylic acid)-coated silver nanoparticles may reach the intestine in a nanoscaled form even if enclosed in a food matrix. While appropriate for studies on the uptake into intestinal cells, the Caco-2 model might be less suited for translocation studies. Moreover, we show that nanoparticle digestion protocols lacking food components may lead to misinterpretation of uptake studies and inconclusive results.

scholarly journals Pharmacological Inhibition of LSD1 for Cancer Treatment

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3194 ◽  
Author(s):  
Guan-Jun Yang ◽  
Pui-Man Lei ◽  
Suk-Yu Wong ◽  
Dik-Lung Ma ◽  
Chung-Hang Leung
Keyword(s):  
Myeloid Leukemia ◽  
Drug Target ◽  
Cancer Cell Proliferation ◽  
Pharmacological Inhibition ◽  
Lysine Specific Demethylase ◽  
Cell Metastasis ◽  
Structural Aspects

Lysine-specific demethylase 1A (LSD1, also named KDM1A) is a demethylase that can remove methyl groups from histones H3K4me1/2 and H3K9me1/2. It is aberrantly expressed in many cancers, where it impedes differentiation and contributes to cancer cell proliferation, cell metastasis and invasiveness, and is associated with inferior prognosis. Pharmacological inhibition of LSD1 has been reported to significantly attenuate tumor progression in vitro and in vivo in a range of solid tumors and acute myeloid leukemia. This review will present the structural aspects of LSD1, its role in carcinogenesis, a comparison of currently available approaches for screening LSD1 inhibitors, a classification of LSD1 inhibitors, and its potential as a drug target in cancer therapy.

In vitro simulation of gastric digestion of milk proteins: comparison between in vitro and in vivo data

1992 ◽  
Vol 40 (2) ◽  
pp. 239-244 ◽  
Author(s):  
Mireille. Yvon ◽  
Sylvie. Beucher ◽  
Pascale. Scanff ◽  
Sylvie. Thirouin ◽  
Jean Pierre. Pelissier
Keyword(s):  
Milk Proteins ◽  
Gastric Digestion ◽  
In Vitro Simulation

Characterization of products from in vivo and in vitro gastric digestion of milk replacers containing whey proteins

1992 ◽  
Vol 40 (8) ◽  
pp. 1367-1374 ◽  
Author(s):  
Isabelle. Caugant ◽  
Mireille. Yvon ◽  
Sylvie. Thirouin ◽  
Helene V. Petit ◽  
Rene. Toullec ◽  
...  
Keyword(s):  
Whey Proteins ◽  
Gastric Digestion ◽  
Milk Replacers

scholarly journals Gastric Emptying and Dynamic In Vitro Digestion of Drinkable Yogurts: Effect of Viscosity and Composition

Nutrients ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 1308 ◽  
Author(s):  
Olivia Ménard ◽  
Marie-Hélène Famelart ◽  
Amélie Deglaire ◽  
Yann Le Gouar ◽  
Sylvie Guérin ◽  
...  
Keyword(s):  
Gastric Emptying ◽  
Whey Proteins ◽  
In Vitro Digestion ◽  
Gastric Digestion ◽  
Sulfur Colloid ◽  
Hydrolysis Of ◽  
Effect Of The Composition ◽  
99Mtc Sulfur Colloid

Gastric emptying of food is mainly driven by the caloric concentration, the rheological properties of the chyme, and the physical state (liquid/solid) of food once in the stomach. The present work investigated: (1) The effect of the composition and the viscosity of drinkable yogurts on gastric emptying in pigs, and (2) the behavior of yogurts during dynamic in vitro digestion. Three isocaloric liquid yogurts were manufactured: Two enriched in protein and fiber showing either a low (LV) or high (HV) viscosity, one control enriched in sugar and starch (CT). They were labelled with 99mTc-sulfur colloid and given to pigs (n = 11) to determine gastric emptying pattern by gamma scintigraphy. Then dynamic in vitro digestion of the yogurts was done using the parameters of gastric emptying determined in vivo. Gastric emptying half-times were significantly longer for LV than CT, whereas HV exhibited an intermediate behavior. In vitro gastric digestion showed a quick hydrolysis of caseins, whereas whey proteins were more resistant in the stomach particularly for LV and HV. During the intestinal phase, both whey proteins and caseins were almost fully hydrolyzed. Viscosity was shown to affect the behavior of yogurt in the small intestine.

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