Transcriptome and metabolome profiling to elucidate mechanisms underlying the blue discoloration of radish roots during storage

BACKGROUND: Urologic guidelines recommend perioperative instillation of chemotherapy after transurethral resection of bladder tumor (TURBT) to decrease tumor recurrence, yet implementation of this recommendation is partial due to associated morbidity. Hypertonic saline destroys cells by osmotic dehydration and might present a safer alternative. OBJECTIVE: To evaluate the safety of 3% hypertonic saline (Hypersal) intravesical instillation following TURBT in rats and in humans. METHODS: In 8 rats whose bladders were electrically injured, intravesical blue-dyed Hypersal was administered. We measured serum sodium levels before and after instillation and pathologically evaluated their pelvic cavity for signs of inflammation or blue discoloration. Twenty-four patients were recruited to the human trial (NIH-NCT04147182), 15 comprised the interventional and 10 the control group (one patient crossed over). Hypersal was given postoperatively. Serum sodium was measured before, 1 hour and 12–24 hours after instillation. Adverse effects were documented and compared between the groups. RESULTS: In rats, average sodium levels were 140.0 mEq/L and 140.3 mEq/L before and following instillation, respectively. Necropsy revealed no signs of inflammation or blue discoloration. In humans the average plasma sodium levels were 138.6 mEq∖L, 138.8 mEq∖L and 137.7 mEq∖L before, 1 hour and 12–24 hours after instillation, respectively. During the postoperative follow-up there was one case of fever. A month after the surgery, dysuria was reported by 5 patients while urgency and hematuria were reported by one patient each. The most severe adverse events were grade 2 on the Clavien-Dindo scale. Adverse events were similar in the control group. CONCLUSIONS: Hypersal instillation is safe and tolerable immediately after TURBT.

Download Full-text

Transcriptome and metabolome profiling in naturally infested Casuarina equisetifolia clones by Ralstonia solanacearum

Genomics ◽

10.1016/j.ygeno.2021.03.022 ◽

2021 ◽

Author(s):

Yongcheng Wei ◽

Yong Zhang ◽

Jingxiang Meng ◽

Yujiao Wang ◽

Chonglu Zhong

Keyword(s):

Ralstonia Solanacearum ◽

Casuarina Equisetifolia ◽

Metabolome Profiling

Download Full-text

Comparative transcriptome and metabolome profiling in the maturing seeds of contrasting cluster bean (Cyamopsis tetragonoloba L. Taub) cultivars identified key molecular variations leading to increased gum accumulation

Gene ◽

10.1016/j.gene.2021.145727 ◽

2021 ◽

pp. 145727

Author(s):

Sudhagar Rajaprakasam ◽

Hifzur Rahman ◽

Shamini Karunagaran ◽

Kannan Bapu ◽

J.R ◽

...

Keyword(s):

Cyamopsis Tetragonoloba ◽

Comparative Transcriptome ◽

Cluster Bean ◽

Metabolome Profiling

Download Full-text

Plasma Metabolome Profiling Identifies Metabolic Subtypes of Pancreatic Ductal Adenocarcinoma

Cells ◽

10.3390/cells10071821 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1821

Author(s):

Ujjwal Mukund Mahajan ◽

Ahmed Alnatsha ◽

Qi Li ◽

Bettina Oehrle ◽

Frank-Ulrich Weiss ◽

...

Keyword(s):

Pancreatic Ductal Adenocarcinoma ◽

Enrichment Analysis ◽

Response Prediction ◽

Ductal Adenocarcinoma ◽

Pathway Enrichment Analysis ◽

Metabolome Analysis ◽

Dismal Prognosis ◽

Chemotherapeutic Response ◽

Plasma Metabolome ◽

Metabolome Profiling

Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers. Developing biomarkers for early detection and chemotherapeutic response prediction is crucial to improve the dismal prognosis of PDAC patients. However, molecular cancer signatures based on transcriptome analysis do not reflect intratumoral heterogeneity. To explore a more accurate stratification of PDAC phenotypes in an easily accessible matrix, plasma metabolome analysis using MxP® Global Profiling and MxP® Lipidomics was performed in 361 PDAC patients. We identified three metabolic PDAC subtypes associated with distinct complex lipid patterns. Subtype 1 was associated with reduced ceramide levels and a strong enrichment of triacylglycerols. Subtype 2 demonstrated increased abundance of ceramides, sphingomyelin and other complex sphingolipids, whereas subtype 3 showed decreased levels of sphingolipid metabolites in plasma. Pathway enrichment analysis revealed that sphingolipid-related pathways differ most among subtypes. Weighted correlation network analysis (WGCNA) implied PDAC subtypes differed in their metabolic programs. Interestingly, a reduced expression among related pathway genes in tumor tissue was associated with the lowest survival rate. However, our metabolic PDAC subtypes did not show any correlation to the described molecular PDAC subtypes. Our findings pave the way for further studies investigating sphingolipids metabolisms in PDAC.

Download Full-text

Transcriptome and metabolome profiling provide insights into molecular mechanism of pseudostem elongation in banana

BMC Plant Biology ◽

10.1186/s12870-021-02899-6 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Guiming Deng ◽

Fangcheng Bi ◽

Jing Liu ◽

Weidi He ◽

Chunyu Li ◽

...

Keyword(s):

Cell Wall ◽

Cell Elongation ◽

Molecular Mechanisms ◽

Specific Gene ◽

Wild Type ◽

Banana Plant ◽

Cell Wall Modification ◽

Dwarf Cultivar ◽

Important Trait ◽

Metabolome Profiling

AbstractBackgroundBanana plant height is an important trait for horticultural practices and semi-dwarf cultivars show better resistance to damages by wind and rain. However, the molecular mechanisms controlling the pseudostem height remain poorly understood. Herein, we studied the molecular changes in the pseudostem of a semi-dwarf banana mutant Aifen No. 1 (Musaspp. Pisang Awak sub-group ABB) as compared to its wild-type dwarf cultivar using a combined transcriptome and metabolome approach.ResultsA total of 127 differentially expressed genes and 48 differentially accumulated metabolites were detected between the mutant and its wild type. Metabolites belonging to amino acid and its derivatives, flavonoids, lignans, coumarins, organic acids, and phenolic acids were up-regulated in the mutant. The transcriptome analysis showed the differential regulation of genes related to the gibberellin pathway, auxin transport, cell elongation, and cell wall modification. Based on the regulation of gibberellin and associated pathway-related genes, we discussed the involvement of gibberellins in pseudostem elongation in the mutant banana. Genes and metabolites associated with cell wall were explored and their involvement in cell extension is discussed.ConclusionsThe results suggest that gibberellins and associated pathways are possibly developing the observed semi-dwarf pseudostem phenotype together with cell elongation and cell wall modification. The findings increase the understanding of the mechanisms underlying banana stem height and provide new clues for further dissection of specific gene functions.

Download Full-text

Metabolome profiling reveals impact of water limitation on grain filling in contrasting rice genotypes

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2021.02.030 ◽

2021 ◽

Vol 162 ◽

pp. 690-698

Author(s):

Preethi Vijayaraghavareddy ◽

Nagashree N. Akula ◽

Ramu S. Vemanna ◽

Raviswamy G.H. Math ◽

Dhananjay D. Shinde ◽

...

Keyword(s):

Grain Filling ◽

Water Limitation ◽

Metabolome Profiling ◽

Rice Genotypes

Download Full-text

Characterization and reactivity of Fe3O4/FeMnOx core/shell nanoparticles for methylene blue discoloration with H2O2

Applied Catalysis B Environmental ◽

10.1016/j.apcatb.2011.08.002 ◽

2011 ◽

Vol 107 (3-4) ◽

pp. 386-392 ◽

Cited By ~ 85

Author(s):

Shengtao Xing ◽

Zicheng Zhou ◽

Zichuan Ma ◽

Yinsu Wu

Keyword(s):

Methylene Blue ◽

Core Shell ◽

Shell Nanoparticles ◽

Blue Discoloration ◽

Core Shell Nanoparticles

Download Full-text

Complication of Ritual Circumcision in Israel

PEDIATRICS ◽

10.1542/peds.54.4.521 ◽

1974 ◽

Vol 54 (4) ◽

pp. 521-521

Author(s):

M. Frand ◽

N. Berant ◽

N. Brand ◽

Y. Rotem

Keyword(s):

Emergency Room ◽

Newborn Infants ◽

Lower Extremities ◽

Unusual Complication ◽

Blue Discoloration ◽

Ritual Circumcision

We have recently had the opportunity to see 3 cases of a very unusual complication of circumcision. Performers of ritual circumcision in Israel wrap the circumcised penis with a firm, circular bandage for hemostasis. The three newborn infants we saw were brought to the emergency room about a day after the procedure because of restlessness, refusal to eat and occasional vomiting. One of the mothers noticed blue discoloration of her child's legs. On examination, cyanosis of both lower extremities and a huge bladder were found in all three infants.

Download Full-text