Identification of tuna species by computer-assisted and cluster analysis of PCR–SSCP electrophoretic patterns

Food Control ◽  
2005 ◽  
Vol 16 (1) ◽  
pp. 51-53 ◽  
Author(s):  
Fabio Colombo ◽  
Gerard Mangiagalli ◽  
Pietro Renon
Keyword(s):  
Cluster Analysis ◽  
Computer Assisted ◽  
Electrophoretic Patterns ◽  
And Cluster Analysis

scholarly journals A New Method of Assessing Sheep Red Blood Cell Types from Their Morphology

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1130 ◽  
Author(s):  
Ivona Žura Žaja ◽  
Silvijo Vince ◽  
Nina Poljičak Milas ◽  
Ingo Ralph Albin Lobpreis ◽  
Branimira Špoljarić ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Image Analysis ◽  
Principal Component ◽  
Morphometric Parameters ◽  
Haematological Parameters ◽  
Computer Assisted ◽  
Corpuscular Haemoglobin ◽  
Computer Assisted Image ◽  
Mean Corpuscular Haemoglobin ◽  
And Cluster Analysis

Data concerning the morphometric parameters of sheep red blood cells (RBCs) obtained using computer-assisted image analysis have not yet been investigated, and there are no data on any analyses of ovine RBC subpopulations based on their morphometric parameters. The aims of this study are to determine the values of RBC haematological and morphometric size and shape parameters, to form groups according to the obtained values of haematological parameters; to determine the differences in RBC morphometric parameters between the formed groups, and to determine RBC subpopulations and their respective proportions in the formed groups. Thirty-six blood samples were collected from the jugular vein of clinically healthy Lika pramenka sheep, aged between 2 and 5 years. Haematological parameters including haemoglobin (HGB), haematocrit (HTC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), and RBC distribution width were analysed using a haematology analyser. Haematological parameters were categorized into two groups: those with lower values or values below the physiological range (Groups 1) and groups with higher values or values above the physiological range (Groups 2). Morphometric parameters of RBCs were determined from stained blood smears using SFORM, a computer-assisted program. Significantly higher values of RBC area, outline, convex, minimal and maximal radius, as well as length and breadth were established in Groups 2 compared to Groups 1 of HGB, HCT, MCV, MCH, and MCHC, respectively. Based on the morphometric parameters of RBCs, three RBC subpopulations were obtained using principal component and cluster analysis: ES 1—the smallest and most elongated RBCs, ES 2—the biggest and most rounded RBCs, and ES 3—average size and shape RBCs. Significantly higher proportions of ES 2 and ES 3 subpopulations, as well as a significantly lower proportion of ES 1 subpopulation, were established in Groups 2 compared to Groups 1 of HGB, HTC, MCV, and MCH, respectively. It can be concluded that ovine RBC subpopulations, based on their morphometric parameters, can be obtained by using computer-assisted image analysis of RBC morphometry and multivariate statistical methods, including principal component and cluster analysis. RBC morphometry, including classification into subpopulations, could serve as a basis for future possibilities in the diagnostic interpretation of anaemic syndromes in veterinary medicine, especially in normocytic, macrocytic, and microcytic anaemias in sheep.

scholarly journals The structure of subpopulations of stallion spermatozoa after thawing differs between good and poor freezers

2020 ◽  
Vol 65 (No. 11) ◽  
pp. 403-410
Author(s):  
Filipa Bubenickova ◽  
Jiří Šichtař ◽  
Linda Nováčková ◽  
Jitka Sirohi ◽  
Ondřej Šimoník
Keyword(s):  
Cluster Analysis ◽  
Sperm Quality ◽  
Computer Assisted ◽  
Motile Sperm ◽  
Kinematic Parameters ◽  
Sperm Analysis ◽  
Total Proportion ◽  
And Cluster Analysis ◽  
The Times ◽  
The Individual

The aim of this study was to evaluate differences in the presence of sperm subpopulations in frozen-thawed semen in stallions with different freezability. The motility of individual spermatozoa of 24 stallions from 15 breeds was evaluated using computer-assisted sperm analysis (CASA) immediately after thawing (T0) and after 30 min of incubation (T30). In accordance with our previous studies, samples were initially divided based on their total motility into categories of good (GF) and poor (PF) freezers. K-means cluster analysis of kinematic parameters of spermatozoa was used to divide motile sperm (n = 57 630) into three subpopulations. Analysis of variance was used to evaluate differences in the subpopulations between GF and PF stallions at the times of incubation T0 and T30. Statistically significant differences were found in most kinematic parameters between PF and GF stallions as well as between the times of incubation T0 and T30 (P < 0.05). Spermatozoa of good freezers are represented more frequently in the fast and medium fast subpopulations and are faster and more linear than those of poor freezers (P < 0.05). Sperm from PF stallions were more strongly affected by longer incubation. The percentage of sperm in the fast and medium fast subpopulations was lower in samples from PF stallions, but assessment of the motility parameters in particular sperm subpopulations revealed that these sperm had good velocity. Poor freezer samples had lower sperm quality due to a reduced total proportion of motile sperm, and these samples were more sensitive to prolonged time after thawing. Thus, an efficient sperm selection method or a special insemination technique should be used for obtaining doses from stallions with poor freezability. Our study showed that the CASA system and cluster analysis are promising tools for better understanding the significant differences in the individual stallion freezability, and further research should be focused on their application in the field.

scholarly journals Negative psychological aspects of working with experimental animals in scientific research

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11035
Author(s):  
Hanna Mamzer ◽  
Agnieszka Zok ◽  
Piotr Białas ◽  
Mirosław Andrusiewicz
Keyword(s):  
Cluster Analysis ◽  
Young People ◽  
Animal Welfare ◽  
Psychological Aspects ◽  
Computer Assisted ◽  
Animal Testing ◽  
Correlation Matrices ◽  
Experimental Animals ◽  
And Cluster Analysis ◽  
Main Component

The aim of the study was to reveal the negative psychological aspects of using animals by scientists and to determine whether the emotional tensions and stress are associated with performing experiments on animals. All 150 participants of the study conduct experiments on animals in their work. Computer-assisted web interviewing, was used to collect the data. Correlation matrices for factorial analysis of main component loads and cluster analysis have been calculated as grouping methods revealed two different categories of researchers, which were mostly distinguished by acceptance and aversion to animal testing and animal welfare. The main findings demonstrated, that there is a group of respondents who feel discomfort when performing experiments on animals. Especially young people involved in animal testing, feel remorse, emotional tension and helplessness.

scholarly journals Segmentation of Coffee Consumers Using Sustainable Values: Cluster Analysis on the Polish Coffee Market

2018 ◽  
Author(s):  
Grzegorz Maciejewski ◽  
Sylwia Mokrysz ◽  
Łukasz Wróblewski
Keyword(s):  
Cluster Analysis ◽  
Sustainable Consumption ◽  
Computer Assisted ◽  
Purchasing Decisions ◽  
Goods And Services ◽  
Long Time ◽  
The Face ◽  
Coffee Market ◽  
And Cluster Analysis ◽  
Source Of Information

In the face of the ongoing degradation of the natural environment and increasingly worrying climate change, societies and their governments should pay more and more attention to the issue of the development of sustainable consumption and pro-environmental consumer behaviour. It has been known for a long time that producers and retailers are the driving force behind adopting the idea of ​​sustainable development. Unfortunately, many of them, when preparing the offer of their goods and services, still take into account only such consumer characteristics as their wealth, the purchasing frequency and volume. In consumer segmentation, the sustainable values ​​that consumers follow when making their purchasing decisions are rarely taken into account. The purpose of the presented article is to try to fill the research gap in this area. The Polish coffee market, on which this type of research has not been conducted so far, was chosen as an example of segmentation taking into account the sustainable values ​​of consumers. The article’s main source of information is the results of primary research carried out using the CAWI (Computer-Assisted Web Interview) technique on a nationwide sample of 800 coffee consumers in July 2018. Multi-dimensional analyses such as extrapolative factor analysis (EFA) and cluster analysis (CA) were used to describe the results which were obtained from the research and statistical analysis. This made it possible to identify and describe six segments of coffee consumers, taking into account their demographic, social and economic characteristics as well as being guided by sustainable values in their purchases. The conclusions presented in the last part of the article may be used by manufacturing and trade enterprises, operating on the coffee market, in order to respond to the identified needs and expectations of consumers and by governmental and social organisations so as to determine the directions of pro-ecological education of consumers.

The limit of the contemporary financial system in Russia

2020 ◽  
Vol 16 (7) ◽  
pp. 1223-1245
Author(s):  
V.V. Smirnov
Keyword(s):  
Cluster Analysis ◽  
Foreign Currency ◽  
Financial System ◽  
Descriptive Statistics ◽  
High Tech ◽  
Monetary Aggregate ◽  
Internal System ◽  
Monetary Relations ◽  
And Cluster Analysis ◽  
Structural Preservation

Subject. The article focuses on the modern financial system of Russia. Objectives. I determine the limit of the contemporary financial system in Russia. Methods. The study is based on methods of descriptive statistics, statistical and cluster analysis. Results. The article shows the possibility of determining the scope of the contemporary financial system in Russia by establishing monetary relations as the order of the internal system and concerted operation of subsystems, preserving the structure of the financial system, maintaining the operational regime, implementing the program and achieving the goal. I found that the Russian financial system correlated with the Angolan one, and the real scope of the contemporary financial system in Russia. Conclusions and Relevance. As an attempt to effectively establish monetary relations and manage them, the limit of the contemporary financial system is related to the possibility of using Monetary Aggregate M0 to maintain the balance of the Central Bank of Russia. To overcome the scope of Russia’s financial system, the economy should have changed its specialization, refocusing it on high-tech export and increasing the foreign currency reserves. This can be done if amendments to Russia’s Constitution are adopted. The findings expand the scope of knowledge and create new competence in the establishment of monetary relations, order of the internal system and concerted interaction of subsystems, structural preservation of the financial system and maintenance of its operational regime.

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