Tracing the food sources of isolated strains of Listeria monocytogenes through fatty acid profiles analysis

Food Control ◽  
2010 ◽  
Vol 21 (8) ◽  
pp. 1092-1098 ◽  
Author(s):  
Ailing Guo ◽  
Ting Liu ◽  
Ji Xie ◽  
Hongjiang Wang ◽  
Hong Yang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Listeria Monocytogenes ◽  
Food Sources ◽  
Fatty Acid Profiles

scholarly journals Increased Isoprenoid Quinone Concentration Modulates Membrane Fluidity inListeria monocytogenesat Low Growth Temperatures

2018 ◽  
Vol 200 (13) ◽  
pp. e00148-18 ◽  
Author(s):  
Waldemar Seel ◽  
Alexander Flegler ◽  
Marija Zunabovic-Pichler ◽  
André Lipski
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Listeria Monocytogenes ◽  
Membrane Fluidity ◽  
Low Temperatures ◽  
Membrane Lipid ◽  
Transition Phase ◽  
Fatty Acid Profiles ◽  
Adaptation Mechanism ◽  
Content Type

ABSTRACTListeria monocytogenesis a food pathogen capable of growing at a broad temperature range from 50°C to refrigerator temperatures. A key requirement for bacterial activity and growth at low temperatures is the ability to adjust the membrane lipid composition to maintain cytoplasmic membrane fluidity. In this study, we confirmed earlier findings that the extents of fatty acid profile adaptation differed betweenL. monocytogenesstrains. We were able to demonstrate for isolates from food that growth rates at low temperatures and resistance to freeze-thaw stress were not impaired by a lower adaptive response of the fatty acid composition. This indicated the presence of a second adaptation mechanism besides temperature-regulated fatty acid synthesis. For strains that showed weaker adaptive responses in their fatty acid profiles to low growth temperature, we could demonstrate a significantly higher concentration of isoprenoid quinones. Three strains even showed a higher quinone concentration after growth at 6°C than at 37°C, which is contradictory to the reduced respiratory activity at lower growth temperatures. Analyses of the membrane fluidityin vivoby measuring generalized polarization and anisotropy revealed modulation of the transition phase. Strains with increased quinone concentrations showed an expanded membrane transition phase in contrast to strains with pronounced adaptations of fatty acid profiles. The correlation between quinone concentration and membrane transition phase expansion was confirmed by suppression of quinone synthesis. A reduced quinone concentration resulted in a narrower transition phase. Expansion of the phase transition zone by increasing the concentration of non-fatty acid membrane lipids is discussed as an additional mechanism improving adaptation to temperature shifts forL. monocytogenesstrains.IMPORTANCEListeria monocytogenesis a foodborne pathogen with an outstanding temperature range for growth. The ability for growth at temperatures close to the freezing point constitutes a serious contamination potential for cold stored food. The only known mechanism of the species for adaptation of membrane fluidity is modification of the membrane fatty acid composition. We were able to demonstrate that, at least for some strains, this adaptation mechanism is supported by regulation of the menaquinone concentration. The increase of this neutral membrane lipid is correlated with fluidization of the membrane under low-temperature conditions and therefore represents a fatty acid-independent mechanism for adaptation to low temperatures.

Trophic niches determined from fatty acid profiles of sympatric coral reef mesopredators

2019 ◽  
Vol 632 ◽  
pp. 159-174 ◽  
Author(s):  
SL Bierwagen ◽  
H Pethybridge ◽  
MR Heupel ◽  
A Chin ◽  
CA Simpfendorfer
Keyword(s):  
Fatty Acid ◽  
Coral Reef ◽  
Fatty Acid Profiles ◽  
Trophic Niches

The assessment of biofilm community composition on plumbing materials in filtered and unfiltered water distribution systems

2003 ◽  
Vol 3 (1-2) ◽  
pp. 187-191
Author(s):  
M.M. Critchley ◽  
N.J. Cromar ◽  
H.J. Fallowfield
Keyword(s):  
Drinking Water ◽  
Fatty Acid ◽  
Community Composition ◽  
Distribution Systems ◽  
Water Distribution ◽  
Water Distribution Systems ◽  
Polymer Materials ◽  
Fatty Acid Profiles ◽  
Water Supplies ◽  
Drinking Water Distribution Systems

Biofilms have been extensively characterised within drinking water distribution systems. However, the significance of materials on biofilm species diversity is not established. This study investigated the community composition of biofilms on plumbing materials receiving filtered and unfiltered water supplies. Biofilms were extracted from polybutylene, polyethylene, cross-linked polyethylene, unplasticised polyvinyl chloride and copper tubes in sampling rigs receiving Murray-Onkaparinga water before or after filtration. Biofilms were extracted and analysed for fatty acid composition using the FAME™ methodology. There were differences in the fatty acid profiles of biofilms and the respective water supplies, indicating differences in the attached and planktonic communities. The results also showed significant differences in the fatty acid profiles of biofilms on the polymer materials compared to copper, suggesting variations in biofilm populations on the different materials. The potential for materials to select for microbial populations has significant implications for the ecology of drinking water biofilms.

scholarly journals Fatty acid profiles of muscle, liver, heart and kidney of Australian prime lambs fed different polyunsaturated fatty acids enriched pellets in a feedlot system

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Hung Van Le ◽  
Don Viet Nguyen ◽  
Quang Vu Nguyen ◽  
Bunmi Sherifat Malau-Aduli ◽  
Peter David Nichols ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Fatty Acid Profiles

scholarly journals Fatty acid profiles of muscle from large yellow croaker (Pseudosciaena crocea R.) of different age

2009 ◽  
Vol 10 (2) ◽  
pp. 154-158 ◽  
Author(s):  
Hong-gang Tang ◽  
Li-hong Chen ◽  
Chao-geng Xiao ◽  
Tian-xing Wu
Keyword(s):  
Fatty Acid ◽  
Large Yellow Croaker ◽  
Fatty Acid Profiles ◽  
Pseudosciaena Crocea

scholarly journals Menaquinone-mediated regulation of membrane fluidity is relevant for fitness of Listeria monocytogenes

2021 ◽  
Author(s):  
Alexander Flegler ◽  
Vanessa Kombeitz ◽  
André Lipski
Keyword(s):  
Fatty Acid ◽  
Listeria Monocytogenes ◽  
Membrane Fluidity ◽  
Low Temperatures ◽  
Lipid Membrane ◽  
Feedback Inhibition ◽  
Aromatic Amino Acids ◽  
Adaptation Effect ◽  
Membrane Composition ◽  
Lower Growth

AbstractListeria monocytogenes is a food-borne pathogen with the ability to grow at low temperatures down to − 0.4 °C. Maintaining cytoplasmic membrane fluidity by changing the lipid membrane composition is important during growth at low temperatures. In Listeria monocytogenes, the dominant adaptation effect is the fluidization of the membrane by shortening of fatty acid chain length. In some strains, however, an additional response is the increase in menaquinone content during growth at low temperatures. The increase of this neutral lipid leads to fluidization of the membrane and thus represents a mechanism that is complementary to the fatty acid-mediated modification of membrane fluidity. This study demonstrated that the reduction of menaquinone content for Listeria monocytogenes strains resulted in significantly lower resistance to temperature stress and lower growth rates compared to unaffected control cultures after growth at 6 °C. Menaquinone content was reduced by supplementation with aromatic amino acids, which led to a feedback inhibition of the menaquinone synthesis. Menaquinone-reduced Listeria monocytogenes strains showed reduced bacterial cell fitness. This confirmed the adaptive function of menaquinones for growth at low temperatures of this pathogen.

Sign in / Sign up

Export Citation Format

Share Document