Low prevalence of mobile colistin-resistance in U.S. meat, catfish, poultry and genomic characterization of a mcr-1 positive Escherichia coli strain

The reintroduction of colistin, a last-resort antibiotic for multidrug-resistant pathogens, resulted in the global spread of plasmid-mediated mobile colistin resistance (mcr) genes. Our study investigated the occurrence of colistin resistance among Escherichia coli isolated from patients with urinary tract infections admitted to a teaching hospital in Egypt. Out of 67 isolates, three isolates were colistin-resistant, having a minimum inhibitory concentration of 4 µg/mL and possessing the mcr-1 gene. A double mechanism of colistin resistance was detected; production of mcr-1 along with amino acid substitution in PmrB (E123D and Y358N) and PmrA (G144S). Broth mating experiments inferred that mcr-1 was positioned on conjugative plasmids. Whole-genome sequencing of EC13049 indicated that the isolate belonged to O23:H4-ST641 lineage and to phylogroup D. The mcr-1-bearing plasmid corresponded to IncHI2 type with a notable similarity to other E. coli plasmids previously recovered from Egypt. The unbanned use of colistin in the Egyptian agriculture sector might have created a potential reservoir for the mcr-1 gene in food-producing animals that spread to humans. More proactive regulations must be implemented to prevent further dissemination of this resistance. This is the first characterization of mcr-1-carrying IncHI2:ST4 plasmid recovered from E. coli of a clinical source in Egypt.

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Faculty Opinions recommendation of Prospective genomic characterization of the German enterohemorrhagic Escherichia coli O104:H4 outbreak by rapid next generation sequencing technology.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.12538961.13766060 ◽

2011 ◽

Author(s):

Martin Maiden

Keyword(s):

Escherichia Coli ◽

Next Generation Sequencing ◽

Enterohemorrhagic Escherichia Coli ◽

Next Generation ◽

Sequencing Technology ◽

Next Generation Sequencing Technology ◽

Generation Sequencing Technology ◽

Genomic Characterization ◽

Generation Sequencing

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Genomic Characterization of New Variant of Hydrogen Sulfide (H2S)-Producing Escherichia coli with Multidrug Resistance Properties Carrying the mcr-1 Gene in China

Antibiotics ◽

10.3390/antibiotics9020080 ◽

2020 ◽

Vol 9 (2) ◽

pp. 80 ◽

Cited By ~ 7

Author(s):

Silpak Biswas ◽

Mohammed Elbediwi ◽

Guimin Gu ◽

Min Yue

Keyword(s):

Escherichia Coli ◽

Hydrogen Sulfide ◽

Multidrug Resistance ◽

Bacterial Infections ◽

Multidrug Resistant ◽

Health Concern ◽

Colistin Resistance ◽

New Variant ◽

Genomic Characterization ◽

Human Infections

Colistin is considered to be a ‘last-resort’ antimicrobial for the treatment of multidrug-resistant Gram-negative bacterial infections. Identification of Enterobacteriaceae, carrying the transferable colistin resistance gene mcr-1, has recently provoked a global health concern. This report presents the first detection of a hydrogen sulfide (H2S)-producing Escherichia coli variant isolated from a human in China, with multidrug resistance (MDR) properties, including colistin resistance by the mcr-1 gene, which could have great implications for the treatment of human infections.

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Cloning and characterization of the eae gene from a dog attaching and effacing Escherichia coli strain 4221

FEMS Microbiology Letters ◽

10.1111/j.1574-6968.1997.tb10295.x ◽

2006 ◽

Vol 148 (2) ◽

pp. 239-245 ◽

Cited By ~ 28

Author(s):

Hongyan An ◽

John M Fairbrother ◽

J.Daniel Dubreuil ◽

Josée Harel

Keyword(s):

Escherichia Coli ◽

Coli Strain

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Characterization of a gne::IS629 O Rough:H7 Escherichia coli Strain from a Hemorrhagic Colitis Patient

Applied and Environmental Microbiology ◽

10.1128/aem.00740-10 ◽

2010 ◽

Vol 76 (15) ◽

pp. 5290-5291 ◽

Cited By ~ 6

Author(s):

Lydia V. Rump ◽

Lothar Beutin ◽

Markus Fischer ◽

Peter C. H. Feng

Keyword(s):

Escherichia Coli ◽

Coli Strain ◽

Hemorrhagic Colitis ◽

Colitis Patient ◽

Pathogenic Potential

ABSTRACT Shiga-toxigenic Escherichia coli strains that are O rough:H7 due to gne::IS629 were thought to be rare and to have unknown pathogenic potential. Recently, an O rough:H7 strain caused by gne::IS629 was isolated from a hemorrhagic colitis patient, suggesting that these strains are pathogenic and may not be as rare as anticipated.

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