Ink melanin from Sepiapharaonis ameliorates colitis in mice via reducing oxidative stress, andprotecting the intestinal mucosal barrier

2021 ◽  
pp. 110888
Author(s):  
Jingwen Xie ◽  
Lin Liu ◽  
Hongyan Li ◽  
Hongxia Che ◽  
Wancui Xie
Keyword(s):  
Oxidative Stress ◽  
Mucosal Barrier ◽  
Intestinal Mucosal Barrier

scholarly journals Low Molecular Seleno-Aminopolysaccharides Protect the Intestinal Mucosal Barrier of Rats under Weaning Stress

2019 ◽  
Vol 20 (22) ◽  
pp. 5727 ◽  
Author(s):  
Zheng-Shun Wen ◽  
Ming Du ◽  
Zhen Tang ◽  
Tian-Yi Zhou ◽  
Zhong-Shan Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Intestinal Mucosa ◽  
Expression Level ◽  
Mucosal Barrier ◽  
Intestinal Damage ◽  
Intestinal Tissue ◽  
Intestinal Mucosal Barrier ◽  
Weaning Stress ◽  
Nrf2 Signaling Pathway

Low molecular seleno-aminopolysaccharide (LSA) was synthesized with sodium selenite and low molecular aminopolysaccharide (LA), which is an organic selenium compound. This study is aimed to investigate the protective effect of LSA on the intestinal mucosal barrier in weaning stress rats by detecting the intestinal tissue morphology and function, mucosal thickness and permeability, the structure of MUC2, antioxidant index, the expression level of intracellular transcription factor NF-E2-related factor 2 (Nrf2), and its related factors. The results showed that LSA significantly increased the height of intestinal villi (p < 0.05) and increased the thickness of intestinal mucosa and the number of goblet cells, which indicated that LSA has a protective effect on the intestinal mucosal barrier that is damaged by weaning. Moreover, LSA significantly reduced the level of DAO, D-LA, and LPS compared with the weaning group (p < 0.05), which indicated that LSA reduced the intestinal damage and permeability of weaning rats. In addition, LSA could increase the number and length of glycans chains and the abundance of acid glycans structures in the MUC2 structure, which indicated that LSA alleviated the changes of intestinal mucus protein structure. LSA significantly increased the levels of GSH-Px, SOD, LDH, and CAT, while it decreased the level of MDA in serum and intestinal tissue, which suggested that LSA significantly enhanced the antioxidant capacity and reduced oxidative stress of weaning rats. RT-PCR results showed that LSA significantly increased the expression level of antioxidant genes (GSH-Px, SOD, Nrf2, HO-1), glycosyltransferase genes (GalNT1, GalNT3, GalNT7) and mucin gene (MUC2) in intestinal mucosa (p < 0.05). The results of western blot showed that the LSA activated the Nrf2 signaling pathway by down-regulating the expression of Keap1and up-regulating the expression of Nrf2, and protected the intestinal mucosa from oxidative stress. Overall, LSA could play a protective role in intestinal mucosal barrier of weaning rats by activating the Nrf2 pathway and alleviating the alnormal change of mucin MUC2.

Autophagy Strengthens Intestinal Mucosal Barrier by Attenuating Oxidative Stress in Severe Acute Pancreatitis

2018 ◽  
Vol 63 (4) ◽  
pp. 910-919 ◽  
Author(s):  
Luqiao Huang ◽  
Yingjian Jiang ◽  
Zhenqing Sun ◽  
Zhengyu Gao ◽  
Jiang Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Acute Pancreatitis ◽  
Severe Acute Pancreatitis ◽  
Mucosal Barrier ◽  
Intestinal Mucosal Barrier

scholarly journals Chronic Kidney Disease Induced Intestinal Mucosal Barrier Damage Associated with Intestinal Oxidative Stress Injury

2016 ◽  
Vol 2016 ◽  
pp. 1-9
Author(s):  
Chao Yu ◽  
Zhen Wang ◽  
Shanjun Tan ◽  
Qiang Wang ◽  
Chunyu Zhou ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Systemic Inflammation ◽  
Mucosal Barrier ◽  
Control Group ◽  
Stress Injury ◽  
Intestinal Mucosal Barrier ◽  
The Status ◽  
Over Time

Background.To investigate whether intestinal mucosal barrier was damaged or not in chronic kidney disease progression and the status of oxidative stress.Methods.Rats were randomized into two groups: a control group and a uremia group. The uremia rat model was induced by 5/6 kidney resection. In postoperative weeks (POW) 4, 6, 8, and 10, eight rats were randomly selected from each group to prepare samples for assessing systemic inflammation, intestinal mucosal barrier changes, and the status of intestinal oxidative stress.Results.The uremia group presented an increase trend over time in the serum tumor necrosis factor-alpha, interleukin-6 (IL-6) and IL-10, serum D-lactate and diamine oxidase, and intestinal permeability, and these biomarkers were significantly higher than those in control group in POW 8 and/or 10. Chiu’s scores in uremia group were also increased over time, especially in POW 8 and 10. Furthermore, the intestinal malondialdehyde, superoxide dismutase, and glutathione peroxidase levels were significantly higher in uremia group when compared with those in control group in POW 8 and/or 10.Conclusions.The advanced chronic kidney disease could induce intestinal mucosal barrier damage and further lead to systemic inflammation. The underlying mechanism may be associated with the intestinal oxidative stress injury.

scholarly journals Emodin Protects Sepsis Associated Damage to the Intestinal Mucosal Barrier Through the VDR/ Nrf2 /HO-1 Pathway

2021 ◽  
Vol 12 ◽  
Author(s):  
Luorui Shang ◽  
Yuhan Liu ◽  
Jinxiao Li ◽  
Guangtao Pan ◽  
Fangyuan Zhou ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Cecal Ligation ◽  
Mucosal Barrier ◽  
Protective Mechanism ◽  
Cecal Ligation And Puncture ◽  
Polygonum Multiflorum ◽  
Intestinal Mucosal Barrier ◽  
Mucosal Barrier Injury ◽  
Tnf Α

Aims: Emodin is an anthraquinone extracted from Polygonum multiflorum, which has potential anti-inflammatory and anti-oxidative stress effects. However, the possible protective mechanism of emodin is unclear. The purpose of this study was to investigate the protective mechanism of emodin against cecal ligation and puncture and LPS-induced intestinal mucosal barrier injury through the VDR/ Nrf2 /HO-1 signaling pathway.Methods: We established a mouse model of sepsis by cecal ligation and puncture (CLP), and stimulated normal intestinal epithelial cells with lipopolysaccharide (LPS). VDR in cellswas down-regulated by small interfering ribonucleic acid (siRNA) technology.Mice were perfused with VDR antagonists ZK168281 to reduce VDR expression and mRNA and protein levels of VDR and downstream molecules were detected in cells and tissue. Inflammation markers (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6)) and oxidative stress markers (superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH)) were measured in serum and intestinal tissueby enzym-linked immunosorbent assay. The expression of VDR in intestinal tissue was detected by immunofluorescence. Histopathological changes were assessed by hematoxylin and eosin staining.Results: In NCM460 cells and animal models, emodin increased mRNA and protein expression of VDR and its downstream molecules. In addition, emodin could inhibit the expressions of TNF-α, IL-6 and MDA in serum and tissue, and increase the levels of SOD and GSH. The protective effect of emodin was confirmed in NCM460 cells and mice, where VDR was suppressed. In addition, emodin could alleviate the histopathological damage of intestinal mucosal barrier caused by cecal ligation and puncture.Conclusion: Emodin has a good protective effect against sepsis related intestinal mucosal barrier injury, possibly through the VDR/ Nrf2 /HO-1 pathway.

Glucagon-Like Peptide-2 Improve Intestinal Mucosal Barrier Function in Aged Rats

2018 ◽  
Vol 22 (6) ◽  
pp. 731-738 ◽  
Author(s):  
Weiying Ren ◽  
Jiayu Wu ◽  
Li Li ◽  
Y. Lu ◽  
Y. Shao ◽  
...  
Keyword(s):  
Barrier Function ◽  
Mucosal Barrier ◽  
Aged Rats ◽  
Intestinal Mucosal Barrier ◽  
Mucosal Barrier Function ◽  
Glucagon Like Peptide

Impact of nanomaterials on intestinal mucosal barrier and its application in treating intestinal diseases

2021 ◽  
Author(s):  
Wenshuai Hao ◽  
Ruitao Cha ◽  
Mingzheng Wang ◽  
Pai Zhang ◽  
Xingyu Jiang
Keyword(s):  
The Body ◽  
Mucosal Barrier ◽  
Intestinal Homeostasis ◽  
Intestinal Mucosal Barrier ◽  
Intestinal Diseases ◽  
Harmful Substances

The intestinal mucosal barrier (IMB) is one of the important barriers to prevent harmful substances and pathogens from entering the body environment and maintain intestinal homeostasis. The dysfunction of IMB...

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