Dental Pulp Mesenchymal Stem Cells for Treatment of Periodontal Disease and its Relationship with Oxidative Stress Markers

Periodontal disease (PD) is one of the main causes of tooth loss and is related to oxidative stress and chronic inflammation. Although different treatments have been proposed in the past, the vast majority do not regenerate lost tissues. In this sense, the use of dental pulp mesenchymal stem cells (DPMSCs) seems to be an alternative for the regeneration of periodontal bone tissue. A quasi-experimental study was conducted in a sample of 22 adults between 55 and 64 years of age with PD, without uncontrolled systemic chronic diseases. Two groups were formed randomly: (i) experimental group (EG) n=11, with a treatment based on DPMSCs; and a (ii) control group (CG) n=11, without a treatment of DPMSCs. Every participant underwent clinical and radiological evaluations and measurement of bone mineral density (BMD) by tomography. Saliva samples were taken as well, to determine the total concentration of antioxidants, superoxide dismutase (SOD), lipoperoxides, and interleukins (IL), before and 6 months after treatment. All subjects underwent curettage and periodontal surgery, the EG had a collagen scaffold treated with DPMSCs, while the CG only had the collagen scaffold placed. The EG with DPMSCs showed an increase in the BMD of the alveolar bone with a borderline statistical significance (baseline 638.82±181.7 vs. posttreatment 781.26±162.2 HU, p=0.09). Regarding oxidative stress and inflammation markers, salivary SOD levels were significantly higher in EG (baseline 1.49±0.96 vs. 2.14±1.12 U/L posttreatment, p<0.05) meanwhile IL1β levels had a decrease (baseline 1001.91±675.5vs. posttreatment 722.3±349.4 pg/ml, p<0.05). Our findings suggest that a DPMSCs treatment based on DPMSCs has both an effect on bone regeneration linked to an increased SOD and decreased levels of IL1β in aging subjects with PD.

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Retrieval of a periodontally compromised tooth by allogeneic grafting of mesenchymal stem cells from dental pulp: A case report

Journal of International Medical Research ◽

10.1177/0300060518773244 ◽

2018 ◽

Vol 46 (7) ◽

pp. 2983-2993 ◽

Cited By ~ 7

Author(s):

Beatriz Hernández-Monjaraz ◽

Edelmiro Santiago-Osorio ◽

Edgar Ledesma-Martínez ◽

Andrés Alcauter-Zavala ◽

Víctor Manuel Mendoza-Núñez

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Periodontal Disease ◽

Dental Pulp ◽

Enzymatic Digestion ◽

Periodontal Pocket ◽

Mineral Density ◽

Culture Dish ◽

Pocket Depth ◽

Lower Premolar

Objective To report a case of successful allogeneic grafting of mesenchymal dental pulp stem cells (DPSCs) as preliminary findings in a patient with periodontal disease enrolled into clinical trial ISRCTN12831118. Methods Mesenchymal stem cells from the dental pulp of a deciduous tooth from a 7-year-old donor were separated from the pulp chamber and processed via enzymatic digestion and centrifugation. DPSCs were passaged and cultured on a 35 × 13 mm culture dish in minimum essential medium-alpha, without supplementation. After reaching 80% confluency, 5 x 106 allogeneic DPSCs in 250 µl phosphate buffered saline were seeded onto a dry scaffold of lyophilized collagen-polyvinylpyrrolidone sponge placed in the left lower premolar area of a 61-year-old patient with periodontal disease. Surgical access to the lower premolar area was achieved using the flap technique. Results At 3 and 6 months following allogeneic graft, the patient showed no sign of rejection and exhibited decreases in tooth mobility, periodontal pocket depth and bone defect area. Bone mineral density had increased at the graft site. Conclusions Regenerative periodontal therapy using DPSCs of allogeneic origin may be a promising treatment for periodontal disease-induced bone defects.

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Effect of periodontal disease on oxidative stress markers in patients with atherosclerosis

Clinical Oral Investigations ◽

10.1007/s00784-021-04144-8 ◽

2021 ◽

Author(s):

Aysegul Sari ◽

Vedat Davutoglu ◽

Emrullah Bozkurt ◽

Ibrahim Levent Taner ◽

Kamile Erciyas

Keyword(s):

Oxidative Stress ◽

Periodontal Disease ◽

Oxidative Stress Markers ◽

Stress Markers

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Proinflammatory and Oxidative Stress Markers in Patients with Periodontal Disease

Mediators of Inflammation ◽

10.1155/2007/45794 ◽

2007 ◽

Vol 2007 ◽

pp. 1-5 ◽

Cited By ~ 58

Author(s):

Ivan Borges Jr. ◽

Emília Addison Machado Moreira ◽

Danilo Wilhem Filho ◽

Tiago Bittencourt de Oliveira ◽

Marcelo Barreto Spirelle da Silva ◽

...

Keyword(s):

Oxidative Stress ◽

Periodontal Disease ◽

Gingival Tissue ◽

Control Group ◽

Myeloperoxidase Activity ◽

Enzymatic Antioxidants ◽

Oxidative Stress Markers ◽

Oxidative Stress Biomarkers ◽

Stress Markers ◽

And Oxidative Stress

Objective. To evaluate the involvement of proinflammatory and oxidative stress markers in gingival tissue in individuals with chronic periodontitis.Subject and methods. Eighteen subjects were divided in two groups: experimental (age52.9±5.0) and control (age51.1±9.6). The activities of enzymatic antioxidants such as catalase, glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase, nonenzymatic antioxidants: total glutathione and reduced glutathione, oxidized glutathione (GSSG), thiobarbituric acid reactive substances (TBARS), and myeloperoxidase activity (MPO) were evaluated in gingival tissues from interproximal sites. Statistical differences between groups were determined by independent Studentttest andP<.05.Results. Individuals with periodontal disease exhibited a significant increase in the activities of MPO, GPx, GST, and also in TBARS and GSSG levels in gingival tissue compared to the control group (P<.05).Conclusion. The results of the present work showed an important correlation between oxidative stress biomarkers and periodontal disease.

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Oxidative stress markers in saliva and periodontal disease status: modulation during pregnancy and postpartum

BMC Infectious Diseases ◽

10.1186/s12879-015-1003-z ◽

2015 ◽

Vol 15 (1) ◽

Cited By ~ 20

Author(s):

Pınar Gümüş ◽

Gülnur Emingil ◽

Veli-Özgen Öztürk ◽

Georgios N. Belibasakis ◽

Nagihan Bostanci

Keyword(s):

Oxidative Stress ◽

Periodontal Disease ◽

Disease Status ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Pregnancy And Postpartum

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Evaluation of Oxidative Stress before and after Using Laser and Photoactivation Therapy as Adjuvant of Non-Surgical Periodontal Treatment in Patients with Rheumatoid Arthritis

Antioxidants ◽

10.3390/antiox10020226 ◽

2021 ◽

Vol 10 (2) ◽

pp. 226

Author(s):

Maria-Alexandra Martu ◽

Petra Surlin ◽

Luminita Lazar ◽

George Alexandru Maftei ◽

Ionut Luchian ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Oxidative Stress ◽

Periodontal Disease ◽

Periodontal Therapy ◽

Periodontal Treatment ◽

Oxidative Stress Markers ◽

Treatment Needs ◽

Disease Treatment ◽

Stress Markers ◽

And Oxidative Stress

(1) Background: The aim of this split-mouth design study was to analyze the clinical periodontal indexes and oxidative stress markers in gingival crevicular fluid modifications after three periodontal disease treatment possibilities (scaling and root planning—SRP; SRP and diode laser—L; SRP and photodynamic therapy—PDT). (2) Methods: The study was conducted on 52 patients: systemically healthy subjects with periodontal disease—non-RA (n = 26); and test group (n = 26) subjects with rheumatoid arthritis and periodontal disease—RA. Clinical periodontal measurements (probing depth—PD; Löe and Silness gingival index—GI; papillary bleeding index—PBI; and periodontal community index of treatment needs—CPITN) and oxidative stress markers (8-hydroxy-2’-deoxyguanosine (8-OHdG) and 4 hydroxynonenal (4-HNE)) were analyzed at baseline (T0), after three sessions of periodontal treatment (T1), and 6 months after treatment (T2). (3) Results: Periodontal therapy improved clinical periodontal measurements and oxidative stress markers in both analyzed groups, with supplementary benefits for laser- and PDT-treated periodontal pockets. (4) Conclusions: The analyzed oxidative stress markers decreased significantly following non-surgical periodontal therapy in both rheumatoid arthritis and systemically healthy patients. All the periodontal disease treatment possibilities analyzed in this study offered clinical and paraclinical improvements; however, the association of laser with SRP and photodisinfection with SRP yielded the best clinical and paraclinical outcomes when compared to SRP alone.

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