scholarly journals Cannabidiol effect on the proteomic profile of keratinocytes isolated from rat skin exposed to UVA and UVB radiation

2021 ◽  
Vol 165 ◽  
pp. 40
Author(s):  
Sinemyiz Atalay ◽  
Agnieszka Gęgotek ◽  
Pedro Domingues ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Proteomic Profile ◽  
Uvb Radiation ◽  
Rat Skin

scholarly journals Antioxidant and Anti-inflammatory Effect of Cannabidiol Contributes to the Decreased Lipid Peroxidation of Keratinocytes of Rat Skin Exposed to UV Radiation

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Anna Jastrząb ◽  
Iwona Jarocka-Karpowicz ◽  
Agnieszka Markowska ◽  
Adam Wroński ◽  
Agnieszka Gęgotek ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Uv Radiation ◽  
Physical Factor ◽  
Uvb Radiation ◽  
Rat Skin ◽  
Metabolic Disturbances ◽  
Skin Cells ◽  
Anti Inflammatory ◽  
Proinflammatory Factors

There is a great need for compounds with antioxidant and anti-inflammatory properties for protection against UV radiation, which is the most prooxidative physical factor that skin cells are exposed to everyday. Therefore, the aim of the study was to evaluate the mechanism of phytocannabinoid-cannabidiol (CBD) action in vivo on lipid metabolism in keratinocytes of rat skin exposed to UVA/UVB radiation. Our results show that CBD protects keratinocytes against the effects of UVA/UVB radiation by reducing lipid peroxidation products: 4-HNE and 8-isoPGF2α. In addition, CBD significantly increases the level of endocannabinoids, such as anandamide, 2-arachidonylglycerol, and palmitoylethanolamide, and the activation of their receptors CB1/2 or TRPV1. The above changes are due to the protective effect of CBD against the UVA/UVB-induced decrease in the level/activity of superoxide dismutase and the components of the thioredoxin and glutathione systems. CBD also increases the in vivo transcriptional activity of Nrf2 and the expression of its Bach1 inhibitor as well as preventing the UVA/UVB-induced increase in the expression of Nrf2 activators p21, p62, p38, and KAP1 and proinflammatory factors such as NFκB and TNFα. By counteracting oxidative stress and changes in lipid structure in keratinocytes, CBD prevents cellular metabolic disturbances, protecting the epidermis against UV damage.

scholarly journals Synergistic Cytoprotective Effects of Rutin and Ascorbic Acid on the Proteomic Profile of 3D-Cultured Keratinocytes Exposed to UVA or UVB Radiation

Nutrients ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 2672 ◽  
Author(s):  
Agnieszka Gęgotek ◽  
Iwona Jarocka-Karpowicz ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Ascorbic Acid ◽  
Synergistic Effect ◽  
Protein Biosynthesis ◽  
Three Dimensional ◽  
Antioxidant Response ◽  
Proteomic Profile ◽  
Uvb Radiation ◽  
Skin Cells ◽  
Protective System ◽  
Skin Damages

The combination of ascorbic acid and rutin, often used in oral preparations, due to antioxidant and anti-inflammatory properties, can be used to protect skin cells against the effects of UV radiation from sunlight. Therefore, the aim of this study was to investigate the synergistic effect of rutin and ascorbic acid on the proteomic profile of UVA and UVB irradiated keratinocytes cultured in a three-dimensional (3D) system. Results showed that the combination of rutin and ascorbic acid protects skin cells against UV-induced changes. In particular, alterations were observed in the expression of proteins involved in the antioxidant response, DNA repairing, inflammation, apoptosis, and protein biosynthesis. The combination of rutin and ascorbic acid also showed a stronger cytoprotective effect than when using either compound alone. Significant differences were visible between rutin and ascorbic acid single treatments in the case of protein carboxymethylation/carboxyethylation. Ascorbic acid prevented UV or rutin-induced protein modifications. Therefore, the synergistic effect of rutin and ascorbic acid creates a potentially effective protective system against skin damages caused by UVA and UVB radiation.

scholarly journals Changes in Lipid Profile of Keratinocytes from Rat Skin Exposed to Chronic UVA or UVB Radiation and Topical Application of Cannabidiol

Antioxidants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1178
Author(s):  
Wojciech Łuczaj ◽  
Maria do Rosário Domingues ◽  
Pedro Domingues ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Oxidative Modification ◽  
Environmental Risk Factor ◽  
Uvb Radiation ◽  
Pla2 Activity ◽  
Rat Skin ◽  
Down Regulation ◽  
The Status ◽  
Antioxidant Effects ◽  
Phospholipid Profile

UV radiation is a well-established environmental risk factor known to cause oxidative stress and disrupt the metabolism of keratinocyte phospholipids. Cannabidiol (CBD) is a phytocannabinoid with anti-inflammatory and antioxidant effects. In this study, we examined changes in the keratinocyte phospholipid profile from nude rat skin exposed to UVA and UVB radiation that was also treated topically with CBD. UVA and UVB radiation promoted up-regulation of phosphatidylcholines (PC), lysophosphatidylcholines (LPC), phosphatidylethanolamines (PE) and down-regulation of sphingomyelin (SM) levels and enhanced the activity of phospholipase A2 (PLA2) and sphingomyelinase (SMase). Application of CBD to the skin of control rats led to down-regulation of SM and up-regulation of SMase activity. After CBD treatment of rats irradiated with UVA or UVB, SM was up-regulated and down-regulated, respectively, while ceramide (CER) levels and SMase activity were down-regulated and up-regulated, respectively. CBD applied to the skin of UV-irradiated rats down-regulated LPC, up-regulated PE and phosphatidylserines (PS) and reduced PLA2 activity. In conclusion, up-regulation of PS may suggest that CBD inhibits their oxidative modification, while changes in the content of PE and SM may indicate a role of CBD in promoting autophagy and improving the status of the transepidermal barrier.

scholarly journals Protective Effects of Acehnese Traditionally Fermented Coconut Oil (Pliek U Oil)1 and its Residue (Pliek U) in Ointment against UV Light Exposure: Studies on Male Wistar Rat Skin (Rattus novergicus)

2021 ◽  
Vol 50 (5) ◽  
pp. 1285-1295
Author(s):  
Nanda Earlia ◽  
Mikyal Bulqiah ◽  
Muslem Muslem ◽  
Taufiq Karma ◽  
Rivansyah Suhendra ◽  
...  
Keyword(s):  
Protective Effect ◽  
Active Ingredient ◽  
Protein Oxidation ◽  
Uv Light ◽  
Light Exposure ◽  
Coconut Oil ◽  
Wistar Rat ◽  
Uvb Radiation ◽  
Rat Skin ◽  
Active Ingredients

A protective effect of Acehnese traditionally fermented coconut oil (Pliek U oil) and its residue (Pliek U) have been studied against UVB radiation. The research was performed by preparing the active ingredient in ointment formulation and protective test in male Wistar rat skin (Rattus novergicus). Four different extraction methods were done to produce four active ingredients, that are Pliek U oil (PUO), ethanolic Pliek U oil extract (EPUOE), ethanolic Pliek U extract (EPUE), and ethanolic hexane residue Pliek U extract (EHRPUE). The protective effect was determined based on the erythema index (EI), melanin index (MI), and level of protein oxidation parameters. The protective level from each active ingredient was studied by PCA analysis. The result showed that the active ingredient could inhibit the redness and protein oxidation in skin tissue. PCA results show that concentration (20 v. 40%) does not affect the protection level of the active ingredients of Pliek U oil, but it does affect the one of Pliek U. Therefore, these active ingredients are potential for sunscreen application.

P113 STUDY OF THE PROTEOMIC PROFILE IN PATIENTS WITH ULCERATIVE COLITIS, ITS CORRELATION WITH DIAGNOSIS AND DISEASE ACTIVITY

2018 ◽  
Vol 154 (1) ◽  
pp. S58
Author(s):  
Salah Badr El-Din ◽  
Ezzat Ali ◽  
Doaa Header ◽  
Pacint Moez ◽  
Mohamed Ibrahim
Keyword(s):  
Ulcerative Colitis ◽  
Disease Activity ◽  
Proteomic Profile

P112 STUDY OF THE PROTEOMIC PROFILE IN PATIENTS WITH CROHN’S DISEASE, ITS CORRELATION WITH DIAGNOSIS AND DISEASE ACTIVITY

2018 ◽  
Vol 154 (1) ◽  
pp. S58
Author(s):  
Salah Badr El-Din ◽  
Ezzat Ahmed ◽  
Doaa Header ◽  
Pacint Moez ◽  
Mohamed Ibrahim
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Disease Activity ◽  
Proteomic Profile

A 3.5-Second Phenomenon in Haemostasis

1973 ◽  
Vol 30 (02) ◽  
pp. 363-370
Author(s):  
D Thilo ◽  
E Böhm
Keyword(s):  
Bleeding Time ◽  
Rat Skin ◽  
Fibrin Formation ◽  
Bleeding Area ◽  
Platelet Thrombus ◽  
Platelet Aggregates ◽  
Primary Platelet ◽  
System Mechanism

SummaryExperiments with injury of the abdominal rat skin were carried out to examine the haemostatic system mechanism in vivo after zero to 30 seconds bleeding time. In the bleeding area only a few platelet aggregates could be found with no primary platelet thrombus. After 3.5 second bleeding time the first fibrin strands have been observed at the site of injury. The hypothesis is put forward that there is a very fast reacting haemostatic mechanism which results in the fibrin formation already at 3.5 seconds.

Photostability Study on Character and Antioxidant Activity of Tomato Extract (Solanum lycopersicum l.) in Nanostructured Lipid Carrier (NLC) and Conventional Creame

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Noorma Rosita ◽  
Dewi Haryadi ◽  
Tristiana Erawati ◽  
Rossa Nanda ◽  
Widji Soeratri
Keyword(s):  
Antioxidant Activity ◽  
Physical Stability ◽  
Nanostructured Lipid Carrier ◽  
Uvb Radiation ◽  
K Value ◽  
Antioxidant Power ◽  
Solanum Lycopersicum L ◽  
Tomato Extract ◽  
Dpph Method ◽  
High Shear Homogenization

The aim of this study was to investigate the ability of NLC in increasing photostability of tomato extract in term of antioxidant activity. Photostability testing on antioxidant activity of samples were conducted by accelerating method using UVB radiation 32.400 joule for 21 hours radiation. Antioxidant activity was measured by DPPH method. NLC was made by High Shear Homogenization (HPH) method at 24000 rpm for 4 cycles, while conventional creame was made by low speed at 400 rpm. The product were characterized include: pH, viscosity, and particle size. There were had difference characters and physical stability. NLC had smaller size, more homogenous and more stable than conventional creame. It was known that stability of antioxidant activity of tomato extract in NLC system higher than in conventional creame. That was showed with k value, as constanta of rate scavenging activity decreasing in antioxidant power between time (Sigma 2-tail less than 0.005) of NLC and conventional creame were: 2.03x10-2 %/hour ±0.08 (3.94) and 4.71x 10-2 %/ hour ±0.23 (4.88) respectively.

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