Extract from the fermented soybean product Natto inhibits Vibrio biofilm formation and reduces shrimp mortality from Vibrio harveyi infection

Abstract Objective The objective of this research were to screen quorum quenching activity compound from phyllosphere bacteria as well as antibiofilm activity against several fish pathogen bacteria such as Aeromonas hydrophila, Streptococcus agalactiae, and Vibrio harveyi. Results We found eight phyllosphere bacteria isolates with potential quorum quenching activity to inhibit Chromobacterium violaceum as indicator bacteria. Crude extracts (20 mg/mL) showed various antibiofilm activity against fish pathogenic bacteria used in this study. Isolate JB 17B showed the highest activity to inhibit biofilm formation of A. hydrophila and V. harveyi, meanwhile isolate JB 3B showed the highest activity to inhibit biofilm of S. agalactiae. From destruction assay, isolate JB 8F showed the highest activity to disrupt biofilm of A. hydrophila isolate JB 20B showed the highest activity to disrupt biofilm of V. harveyi, isolate JB 17B also showed the highest activity to disrupt biofilm of S. agalactiae.

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An integrated metagenomic/metaproteomic investigation of microbiota in dajiang-meju, a traditional fermented soybean product in Northeast China

Food Research International ◽

10.1016/j.foodres.2018.10.076 ◽

2019 ◽

Vol 115 ◽

pp. 414-424 ◽

Cited By ~ 18

Author(s):

Mengxi Xie ◽

Junrui Wu ◽

Feiyu An ◽

Xiqing Yue ◽

Dongbing Tao ◽

...

Keyword(s):

Northeast China ◽

Soybean Product ◽

Fermented Soybean

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A Mutation in the luxS Gene Influences Listeria monocytogenes Biofilm Formation

Applied and Environmental Microbiology ◽

10.1128/aem.00048-06 ◽

2006 ◽

Vol 72 (8) ◽

pp. 5653-5658 ◽

Cited By ~ 59

Author(s):

Shlomo Sela ◽

Shmulik Frank ◽

Eddy Belausov ◽

Riky Pinto

Keyword(s):

Listeria Monocytogenes ◽

Biofilm Formation ◽

Parent Strain ◽

Vibrio Harveyi ◽

Reporter Strain ◽

Luxs Gene ◽

Wild Type ◽

Autoinducer 2 ◽

Wild Type Phenotype ◽

Better Than

ABSTRACT Using a Vibrio harveyi reporter strain, we demonstrated that Listeria monocytogenes secretes a functional autoinducer 2 (AI-2)-like signal. A luxS-deficient mutant produced a denser biofilm and attached to a glass surface 19-fold better than the parent strain. Exogenous AI-2 failed to restore the wild-type phenotype to the mutant. It seems that an intact luxS gene is associated with repression of components required for attachment and biofilm formation.

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Fermented soybean product (Cheonggukjang) improved some attributes of protein and growth hormone measurements in Sprague-Dawley rats

Nutrition Research ◽

10.1016/j.nutres.2014.02.004 ◽

2014 ◽

Vol 34 (4) ◽

pp. 355-367 ◽

Cited By ~ 4

Author(s):

In Sik Hwang ◽

Ji Eun Kim ◽

Young Ju Lee ◽

Moon Hwa Kwak ◽

Jun Go ◽

...

Keyword(s):

Growth Hormone ◽

Sprague Dawley ◽

Soybean Product ◽

Fermented Soybean ◽

Sprague Dawley Rats

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Citrus limonoids interfere with Vibrio harveyi cell–cell signalling and biofilm formation by modulating the response regulator LuxO

Microbiology ◽

10.1099/mic.0.041228-0 ◽

2011 ◽

Vol 157 (1) ◽

pp. 99-110 ◽

Cited By ~ 58

Author(s):

Amit Vikram ◽

Palmy R. Jesudhasan ◽

G. K. Jayaprakasha ◽

Suresh D. Pillai ◽

Bhimanagouda S. Patil

Keyword(s):

Biofilm Formation ◽

Furan Ring ◽

Insect Pests ◽

Vibrio Harveyi ◽

Response Regulator ◽

Cell Signalling ◽

Plant Defence ◽

Significant Inhibition ◽

Regulator Gene ◽

Cell Cell

Citrus limonoids are unique secondary metabolites, characterized by a triterpenoid skeleton with a furan ring. Studies have demonstrated beneficial health properties of limonoids. In addition, certain citrus limonoids play a role in plant defence against insect pests. In the present study, five limonoids were purified from sour orange and evaluated for their ability to inhibit cell–cell signalling. The purified limonoids were tested for their ability to interfere with cell–cell signalling and biofilm formation in Vibrio harveyi. Isolimonic acid, deacetylnomilinic acid glucoside and ichangin demonstrated significant inhibition of autoinducer-mediated cell–cell signalling and biofilm formation. Furthermore, isolimonic acid and ichangin treatment resulted in induced expression of the response regulator gene luxO. In addition, luxR promoter activity was not affected by isolimonic acid or ichangin. Therefore, the ability of isolimonic acid and ichangin to interfere with cell–cell signalling and biofilm formation seems to stem from the modulation of luxO expression. The results suggest that isolimonic acid and ichangin are potent modulators of bacterial cell–cell signalling.

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