ALA16VAL-MnSOD gene polymorphism and stroke: Association with dyslipidemia and glucose levels

Leptin G-2548A Gene Polymorphism is Positively Associated with Increased Plasma Leptin and Glucose Levels in Obese Saudi Patients Irrespective Status of Blood Pressure

10.21203/rs.3.rs-148642/v2 ◽

2021 ◽

Author(s):

Lotfi S. Bin Dahman ◽

Nasser M. Al-Daghri

Keyword(s):

Blood Pressure ◽

Gene Polymorphism ◽

Plasma Glucose ◽

Hdl Cholesterol ◽

Cross Sectional Study ◽

Cross Sectional ◽

Glucose Levels ◽

Lep Gene ◽

Plasma Leptin ◽

Saudi Patients

Abstract The association between LEP G-2548A gene polymorphism with increased plasma leptin and glucose levels and blood pressure in a sample of obese Saudi patients has been evaluated. This is a cross-sectional study involved 206 Saudi adult subjects (94 males and 112 females), randomly selected from the primary health care centers, Riyadh, Saudi Arabia. The study sample was categorized into three groups: 50 normotensive ND controls (age: 47.9±5.4 yr.; BMI 22.9±2.1 Kg/m2), 80 obese normotensive ND (age: 47.7±6.0 yr.; BMI 34.1±4.2 Kg/m2) and 76 obese hypertensive with T2D patients (age: 49.4±5.9 yr.; BMI: 35.1±4.7 Kg/m2). Analyses of LEP G-2548A gene polymorphism were determined using polymerase chain reaction (PCR), followed by restriction fragment length polymorphism (RFLP) with 2U of HhaI restriction enzyme. Plasma leptin and insulin levels were measured using the Luminex instrument. Fasting plasma glucose, total cholesterol, HDL-cholesterol, and triglycerides were measured using a chemical autoanalyzer Konelab machine. Also, blood pressure and anthropometric data were measured. The association analysis with metabolic parameters showed that homozygous AA of the LEP gene had significantly higher plasma glucose levels and HOMA-IR compared with homozygous GG (6.8±0.55 vs. 5.8±0.30; p< 0.04; 4.1±0.84 vs. 2.6±0.67; p=0.03) respectively. Besides, heterozygous GA had significantly higher plasma leptin levels compared with homozygous GG (40.0±2.6 vs. 29.6±2.6; P= 0.04). GA, AA, GA+AA genotypes of the LEP G-2548A gene polymorphism are more prevalent among individuals with hyperglycemia (OR= 3.7, 95% CI= 1.6 to 8.4, P= 0.001; OR= 3.2, 95% CI= 1.2 to 8.6, P= 0.03; OR= 3.5, 95%CI= 1.6 to 7.7, P= 0.001) respectively. A allele of the LEP gene is more prevalent among subjects with hyperglycemia (OR= 1.9, 95%CI= 1.2 to 3.0, P=0.006). G-2548A variant of the LEP gene may not be considered as a genetic risk factor for hypertension in Saudi obese patients. However, the genotypes (GA and AA) and -2548AA allele of this gene may represent important risk factors predisposing healthy subjects to develop T2DM irrespective of the status of blood pressure.

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The association between the melatonin receptor 1B gene polymorphism rs10830963 and glucose levels in type 2 diabetes

The Egyptian Journal of Internal Medicine ◽

10.4103/1110-7782.148120 ◽

2014 ◽

Vol 26 (4) ◽

pp. 145

Author(s):

Noha KhM Khalil ◽

OlfatG Shaker ◽

TarekS Heshmat ◽

HebaSh Kareem

Keyword(s):

Type 2 Diabetes ◽

Gene Polymorphism ◽

Melatonin Receptor ◽

Glucose Levels ◽

Melatonin Receptor 1B

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Polimorfisme V16A Gen MnSOD pada Penderita Diabetes Melitus Tipe 2 dengan Retinopati

Journal of Tropical Biodiversity and Biotechnology ◽

10.22146/jtbb.16446 ◽

2018 ◽

Vol 3 (2) ◽

pp. 37

Author(s):

Tasmini Tasmini ◽

R. Haryo Yudono ◽

Maliyah Madyan

Keyword(s):

Oxidative Stress ◽

Diabetic Retinopathy ◽

Gene Polymorphism ◽

Proliferative Diabetic Retinopathy ◽

Stress Hyperglycemia ◽

Type 2 Dm ◽

Pcr Rflp ◽

Group 2 ◽

Mnsod Gene

Complications of diabetes mellitus (DM) include diabetic retinopathy (RD) both non-proliferative diabetic retinopathy (NPDR) and proliferative diabetic retinopathy (PDR). The development of RD depends on environmental and genetic factors. MnSOD gene (manganese-superoxid dismutase) is one of the candidate risk factors gene for RD. The presence of V16A MnSOD gene polymorphism results in decrease of mitochondrial MnSOD enzymes expression and triggers the oxidative stress. Hyperglycemia in DM increases oxidative stress in tissues, including the retina resulting in metabolic abnormalities in the retina, which play a role in the development of DM complications, namely diabetic retinopathy. In Indonesia, especially the Javanese tribes in Yogyakarta, there has never been any research on MnSOD gene polymorphism in type 2 diabetes patients with and without retinopathy. Subjects were Poly Endocrine patients and Eye Polyclinic patients of Dr. Sardjito’s General Hospital, 121 subjects consisting of 63 type 2 DM patients without retinopathy were group 1 (KI) and 58 type 2 DM patients with retinopathy were group 2 (KII) (20 NPDR subjects and 38 PDR subjects). V16A polymorphism of MnSOD gene from leukocytes DNA was analyzed by PCR-RFLP method. From 121 DM subjects, 70 subjects with VV genotype were found, 50 subjects with VA genotype and 1 subject with AA genotype. From 63 non-RD DM subjects, 22 subjects with VA genotypes and 41 subjects with VV genotype were found, while in DM with retinopathy (non-PDR, n = 20) found 6 subjects with VA genotype and 14 subjects with VV genotype, and in DM with retinopathy (PDR, n = 38) found 1 subject with AA genotype, 22 subjects with VA genotype and 15 subjects with VV genotype. In DM with retinopathy (NPDR and PDR, n = 58), 1 subject was found with AA genotype, 27 subjects with VA genotype and 29 subjects with VV genotype.

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Relationship between MTNR1B (melatonin receptor 1B gene) polymorphism rs10830963 and glucose levels in overweight children and adolescents†

Pediatric Diabetes ◽

10.1111/j.1399-5448.2010.00738.x ◽

2011 ◽

Vol 12 (4pt2) ◽

pp. 435-441 ◽

Cited By ~ 8

Author(s):

Thomas Reinehr ◽

André Scherag ◽

Hai-Jun Wang ◽

Christian L Roth ◽

Michaela Kleber ◽

...

Keyword(s):

Gene Polymorphism ◽

Children And Adolescents ◽

Overweight Children ◽

Melatonin Receptor ◽

Glucose Levels ◽

Melatonin Receptor 1B

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Relationship between Superoxide Dismutase Manganese Gene Polymorphism and Eye Tumors

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2021.5968 ◽

2021 ◽

Vol 9 (A) ◽

pp. 229-232

Author(s):

Rodiah Rahmawaty Lubis ◽

Cut Adeya Adella ◽

Lokot Donna Lubis

Keyword(s):

Superoxide Dismutase ◽

Gene Polymorphism ◽

Gene Polymorphisms ◽

Cancer Biology ◽

Orbital Tumor ◽

Control Group ◽

Orbital Tumors ◽

The Impact ◽

The Relationship ◽

Mnsod Gene

ABSTRACT Background: Orbital tumor in Indonesia is one of the eye health problems that can cause blindness. The impact caused by orbital tumors on patients is quite large because it can result in blindness and even death due to its metastatic nature. The role that SOD plays in cancer biology is not well understood, most studies showing a more oxidative state, characterized by increased intracellular ROS, particularly superoxide. Objective: To determine the relationship between Manganese Superoxide Dismutase (SOD2) gene polymorphisms and the incidence of orbital tumors in Medan. Methods: This study is an analytic observational study with a cross-sectional data collection method using controls. Comparisons were made between the control group and the observed group to see the relationship between SOD2 polymorphisms and the risk of orbital tumor incidence in Medan. The ophthalmic examination, anterior and posterior segments, and assessment of CT orbit if deemed necessary for the orbital tumor patients. Histopathological examination was done by the Pathologist. Blood samples was taken for polymorphism examination on extracted DNA using the Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) methods. Results: About 30 patients that met the inclusion criterias. Laterality, the left eye is more likely to suffer from tumors when compared to the right eye. This study found as many as 16 patients, while malignant tumors was 14 patients. There was a relationship between the MnSOD gene polymorphism and the incidence of orbital tumors (p <0.001), there was a relationship between the MnSOD gene polymorphism and the incidence of orbital tumors in the female sex (p <0.001) Conclusion: There was a relationship between MnSOD gene polymorphisms and the incidence of orbital tumors (p <0.001)

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Leptin G-2548A Gene Polymorphism is Positively Associated with Increased Plasma Leptin and Glucose Levels in Obese Saudi Patients Irrespective Status of Blood Pressure

10.21203/rs.3.rs-148642/v3 ◽

2021 ◽

Author(s):

Lotfi S. Bin Dahman ◽

Nasser M. Al-Daghri

Keyword(s):

Blood Pressure ◽

Gene Polymorphism ◽

Plasma Glucose ◽

Hdl Cholesterol ◽

Cross Sectional Study ◽

Cross Sectional ◽

Glucose Levels ◽

Lep Gene ◽

Plasma Leptin ◽

Saudi Patients

Abstract The association between LEP G-2548A gene polymorphism with increased plasma leptin and glucose levels and blood pressure in a sample of obese Saudi patients has been evaluated. This is a cross-sectional study involved 206 Saudi adult subjects (94 males and 112 females), randomly selected from the primary health care centers, Riyadh, Saudi Arabia. The study sample was categorized into three groups: 50 normotensive ND controls (age: 47.9±5.4 yr.; BMI 22.9±2.1 Kg/m2), 80 obese normotensive ND (age: 47.7±6.0 yr.; BMI 34.1±4.2 Kg/m2) and 76 obese hypertensive with T2D patients (age: 49.4±5.9 yr.; BMI: 35.1±4.7 Kg/m2). Analyses of LEP G-2548A gene polymorphism were determined using polymerase chain reaction (PCR), followed by restriction fragment length polymorphism (RFLP) with 2U of HhaI restriction enzyme. Plasma leptin and insulin levels were measured using the Luminex instrument. Fasting plasma glucose, total cholesterol, HDL-cholesterol, and triglycerides were measured using a chemical autoanalyzer Konelab machine. Also, blood pressure and anthropometric data were measured. The association analysis with metabolic parameters showed that homozygous AA of the LEP gene had significantly higher plasma glucose levels and HOMA-IR compared with homozygous GG (6.8±0.55 vs. 5.8±0.30; p< 0.04; 4.1±0.84 vs. 2.6±0.67; p=0.03) respectively. Besides, heterozygous GA had significantly higher plasma leptin levels compared with homozygous GG (40.0±2.6 vs. 29.6±2.6; P= 0.04). GA, AA, GA+AA genotypes of the LEP G-2548A gene polymorphism are more prevalent among individuals with hyperglycemia (OR= 3.7, 95% CI= 1.6 to 8.4, P= 0.001; OR= 3.2, 95% CI= 1.2 to 8.6, P= 0.03; OR= 3.5, 95%CI= 1.6 to 7.7, P= 0.001) respectively. A allele of the LEP gene is more prevalent among subjects with hyperglycemia (OR= 1.9, 95%CI= 1.2 to 3.0, P=0.006). G-2548A variant of the LEP gene may not be considered as a genetic risk factor for hypertension in Saudi obese patients. However, the genotypes (GA and AA) and -2548AA allele of this gene may represent important risk factors predisposing healthy subjects to develop T2DM irrespective of the status of blood pressure.

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