Occurrence of blaOXA-48 type carbapenemase in Escherichia coli with coexisting resistance determinants: A report from India

ABSTRACT The effects of feed supplementation with the approved antimicrobial agents bambermycin, penicillin, salinomycin, and bacitracin or a combination of salinomycin plus bacitracin were evaluated for the incidence and distribution of antibiotic resistance in 197 commensal Escherichia coli isolates from broiler chickens over 35 days. All isolates showed some degree of multiple antibiotic resistance. Resistance to tetracycline (68.5%), amoxicillin (61.4%), ceftiofur (51.3%), spectinomycin (47.2%), and sulfonamides (42%) was most frequent. The levels of resistance to streptomycin, chloramphenicol, and gentamicin were 33.5, 35.5, and 25.3%, respectively. The overall resistance levels decreased from day 7 to day 35 (P < 0.001). Comparing treatments, the levels of resistance to ceftiofur, spectinomycin, and gentamicin (except for resistance to bacitracin treatment) were significantly higher in isolates from chickens receiving feed supplemented with salinomycin than from the other feeds (P < 0.001). Using a DNA microarray analysis capable of detecting commonly found antimicrobial resistance genes, we characterized 104 tetracycline-resistant E. coli isolates from 7- to 28-day-old chickens fed different growth promoters. Results showed a decrease in the incidence of isolates harboring tet(B), bla TEM, sulI, and aadA and class 1 integron from days 7 to 35 (P < 0.01). Of the 84 tetracycline-ceftiofur-resistant E. coli isolates, 76 (90.5%) were positive for bla CMY-2. The proportions of isolates positive for sulI, aadA, and integron class 1 were significantly higher in salinomycin-treated chickens than in the control or other treatment groups (P < 0.05). These data demonstrate that multiantibiotic-resistant E. coli isolates can be found in broiler chickens regardless of the antimicrobial growth promoters used. However, the phenotype and the distribution of resistance determinants in E. coli can be modulated by feed supplementation with some of the antimicrobial agents used in broiler chicken production.

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Genetic but No Phenotypic Associations between Biocide Tolerance and Antibiotic Resistance in Escherichia coli from German Broiler Fattening Farms

Microorganisms ◽

10.3390/microorganisms9030651 ◽

2021 ◽

Vol 9 (3) ◽

pp. 651

Author(s):

Alice Roedel ◽

Szilvia Vincze ◽

Michaela Projahn ◽

Uwe Roesler ◽

Caroline Robé ◽

...

Keyword(s):

Escherichia Coli ◽

Antibiotic Resistance ◽

Acquired Resistance ◽

Antibiotic Resistance Genes ◽

Animal Husbandry ◽

Metal Resistance ◽

Resistant Bacteria ◽

Virulence Determinants ◽

Phylogenetic Distribution ◽

Resistance Determinants

Biocides are frequently applied as disinfectants in animal husbandry to prevent the transmission of drug-resistant bacteria and to control zoonotic diseases. Concerns have been raised, that their use may contribute to the selection and persistence of antimicrobial-resistant bacteria. Especially, extended-spectrum β-lactamase- and AmpC β-lactamase-producing Escherichia coli have become a global health threat. In our study, 29 ESBL-/AmpC-producing and 64 NON-ESBL-/AmpC-producing E.coli isolates from three German broiler fattening farms collected in 2016 following regular cleaning and disinfection were phylogenetically characterized by whole genome sequencing, analyzed for phylogenetic distribution of virulence-associated genes, and screened for determinants of and associations between biocide tolerance and antibiotic resistance. Of the 30 known and two unknown sequence types detected, ST117 and ST297 were the most common genotypes. These STs are recognized worldwide as pandemic lineages causing disease in humans and poultry. Virulence determinants associated with extraintestinal pathogenic E.coli showed variable phylogenetic distribution patterns. Isolates with reduced biocide susceptibility were rarely found on the tested farms. Nine isolates displayed elevated MICs and/or MBCs of formaldehyde, chlorocresol, peroxyacetic acid, or benzalkonium chloride. Antibiotic resistance to ampicillin, trimethoprim, and sulfamethoxazole was most prevalent. The majority of ESBL-/AmpC-producing isolates carried blaCTX-M (55%) or blaCMY-2 (24%) genes. Phenotypic biocide tolerance and antibiotic resistance were not interlinked. However, biocide and metal resistance determinants were found on mobile genetic elements together with antibiotic resistance genes raising concerns that biocides used in the food industry may lead to selection pressure for strains carrying acquired resistance determinants to different antimicrobials.

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Interrelationship between tetracycline resistance determinants, phylogenetic group affiliation and carriage of class 1 integrons in commensal Escherichia coli isolates from cattle farms

BMC Veterinary Research ◽

10.1186/s12917-018-1661-3 ◽

2018 ◽

Vol 14 (1) ◽

Cited By ~ 5

Author(s):

Kuastros Mekonnen Belaynehe ◽

Seung Won Shin ◽

Han Sang Yoo

Keyword(s):

Escherichia Coli ◽

Tetracycline Resistance ◽

Phylogenetic Group ◽

Group Affiliation ◽

Class 1 Integrons ◽

Resistance Determinants ◽

Class 1 ◽

Cattle Farms

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Susceptibility of Escherichia coli and Enterococcus faecium isolated from pigs and broiler chickens to tetracycline degradation products and distribution of tetracycline resistance determinants in E. coli from food animals

Veterinary Microbiology ◽

10.1016/s0378-1135(03)00123-8 ◽

2003 ◽

Vol 95 (1-2) ◽

pp. 91-101 ◽

Cited By ~ 56

Author(s):

Gitte Sengeløv ◽

Bent Halling-Sørensen ◽

Frank M. Aarestrup

Keyword(s):

Escherichia Coli ◽

Enterococcus Faecium ◽

Broiler Chickens ◽

Degradation Products ◽

Tetracycline Resistance ◽

E Coli ◽

Resistance Determinants ◽

Tetracycline Degradation ◽

Food Animals

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High Prevalence of Plasmid-Mediated Quinolone Resistance Determinants qnr, aac(6′)-Ib-cr, and qepA among Ceftiofur-Resistant Enterobacteriaceae Isolates from Companion and Food-Producing Animals

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00886-08 ◽

2008 ◽

Vol 53 (2) ◽

pp. 519-524 ◽

Cited By ~ 134

Author(s):

Junying Ma ◽

Zhenling Zeng ◽

Zhangliu Chen ◽

Xiaogang Xu ◽

Xiaoying Wang ◽

...

Keyword(s):

Escherichia Coli ◽

Klebsiella Pneumoniae ◽

Companion Animals ◽

Pcr Amplification ◽

Guangdong Province ◽

Quinolone Resistance ◽

Resistance Determinants ◽

The Family ◽

High Prevalence ◽

Few Data

ABSTRACT Three kinds of plasmid-mediated quinolone resistance (PMQR) determinants have been discovered and have been shown to be widely distributed among clinical isolates: qnr genes, aac(6′)-Ib-cr, and qepA. Few data on the prevalence of these determinants in strains from animals are available. The presence of PMQR genes in isolates from animals was determined by PCR amplification and DNA sequencing. The production of extended-spectrum β-lactamases (ESBLs) and AmpC β-lactamases in the strains was detected, and their genotypes were determined. The genetic environment of PMQR determinants in selected plasmids was analyzed. All samples of ceftiofur-resistant (MICs ≥ 8 μg/ml) isolates of the family Enterobacteriaceae were selected from 36 companion animals and 65 food-producing animals in Guangdong Province, China, between November 2003 and April 2007, including 89 Escherichia coli isolates, 9 Klebsiella pneumoniae isolates, and isolates of three other genera. A total of 68.3% (69/101) of the isolates produced ESBLs and/or AmpC β-lactamases, mainly those of the CTX-M and CMY types. Of the 101 strains, PMQR determinants were present in 35 (34.7%) isolates, with qnr, aac(6′)-Ib-cr, and qepA detected alone or in combination in 8 (7.9%), 19 (18.8%), and 16 (15.8%) strains, respectively. The qnr genes detected included one qnrB4 gene, four qnrB6 genes, and three qnrS1 genes. Five strains were positive for both aac(6′)-Ib-cr and qepA, while one strain was positive for qnrS1, aac(6′)-Ib-cr, and qepA. qnrB6 was flanked by two copies of ISCR1 with an intervening dfr gene downstream and sul1 and qacEΔ1 genes upstream. In another plasmid, aac(6′)-Ib-cr followed intI1 and arr-3 was downstream. PMQR determinants are highly prevalent in ceftiofur-resistant Enterobacteriaceae strains isolated from animals in China. This is the first report of the occurrence of PMQR determinants among isolates from companion animals.

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Determinants of β-lactam resistance in meningitis-causingEnterobacteriaceaein Brazil

Canadian Journal of Microbiology ◽

10.1139/w10-020 ◽

2010 ◽

Vol 56 (5) ◽

pp. 399-407 ◽

Cited By ~ 16

Author(s):

L. N. Andrade ◽

L. A.R. Minarini ◽

A. Pitondo-Silva ◽

E. C. Clímaco ◽

I. C.V. Palazzo ◽

...

Keyword(s):

Escherichia Coli ◽

Insertion Sequence ◽

Genetic Relationships ◽

Mlst Database ◽

High Genetic Diversity ◽

Mlst Analysis ◽

E Coli ◽

Resistance Determinants ◽

Class 1 ◽

Encoding Genes

This study analyzed resistance determinants in extended-spectrum β-lactamase (ESBL)-producing enterobacteria and the epidemiology of 11 Escherichia coli isolates obtained from meningitis patients in a region of Brazil from 2000 to 2005. ESBL-encoding genes and their genetic environment were investigated by PCR and sequencing. The gene blaCTX-M-2was identified in 3 different enterobacteria (E. coli, Serratia marcescens , and Proteus mirabilis ) downstream of the insertion sequence ISCR1 (localized in class 1 integrons), but not as part of the resistance cassettes region. Multilocus sequence typing (MLST) was used to investigate genetic relationships between the 11 E. coli isolates in this study and strains associated with meningitis in the E. coli MLST database. MLST analysis indicated high genetic diversity among isolates, and no significant genetic relationship was identified with meningitis-causing E. coli in the database. The results in this report reinforce the need to be attentive to meningitis suspected to be due to ESBL-producing enterobacterial isolates, especially where ESBL epidemiology is well known.

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