In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression

Ficus deltoidea (Mas Cotek) is one of the popular herbs that has been used traditionally to alleviate illnesses. The present study aimed to investigate the in vitro wound healing activity of F. deltoidea leaf extract on skin cell. Cell proliferative and migration assay were done on the Human Skin Fibroblast cell (HSF 1184) which were treated with different concentrations of F. deltoidea leaf extract. The data of wound closure were collected at time intervals of 0, 6, 12 and 24 hours and analyzed using ImageJ™ software. MTT assay revealed that the F. deltoidea leaf extract could induce cell proliferation at a dose dependent manner. F. deltoidea leaf extract significantly accelerated the wound closure process in comparison to cells treated with ascorbic acid and untreated cells in scratch assay. Following 24 hours of incubation, cells treated with 50 mg/mL of F. deltoidea leaf extract showed remarkable proliferative and wound closure effect with 143.67% and 5.96%, respectively as compared to other concentrations. Collectively, these findings suggested that F. deltoidea leaf extract possesses wound healing potential and may be useful for the development of efficient wound healing drug.

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Influence of phytochemicals in piper betle linn leaf extract on wound healing

Burns & Trauma ◽

10.1186/s41038-015-0023-7 ◽

2015 ◽

Vol 3 ◽

pp. 1-8 ◽

Cited By ~ 5

Author(s):

Le Thi Lien ◽

Nguyen Thi Tho ◽

Do Minh Ha ◽

Pham Luong Hang ◽

Phan Tuan Nghia ◽

...

Keyword(s):

Wound Healing ◽

Leaf Extract ◽

Lipid Hydroperoxide ◽

Piper Betle ◽

Burn Wound ◽

Nih3t3 Cells ◽

Cutaneous Wounds ◽

Scratch Wound

Abstract Background Wound healing has being extensively investigated over the world. Healing impairment is caused by many reasons including increasing of free-radicals-mediated damage, delaying in granulation tissue formation, reducing in angiogenesis and decreasing in collagen reorganization. These facts consequently lead to chronic wound healing. Piper betle Linn (Betle) leaves have been folklore used as an ingredient of drugs for cutaneous wound treatment. However, the effect of betle leaf on wound healing is not yet well elucidated. In this study, we aimed to investigate the healing efficacy of methanol leaf extract of Piper betle Linn on proliferation of fibroblast NIH3T3 cells as well as full-thickness burn and excision wounds in swiss mice. Methods Scratch wound healing assays were conducted to examine the effects of betle leaf extract on healing activity of fibroblast cells. Burn and excision wounds on swiss mouse skins were created for investigating the wound healing progress caused by the betle leaf extract. Malondialdehyde (MDA) was also evaluated to examine the products of lipid hydroperoxide (LPO) under conditions of with or without betle leaf extract treatment. Results The results of this study showed that Piper betle Linn leaf extract in methanol increased proliferation of NIH3T3 cells and promoted wound healing in vitro and in vivo with both burn wound and excision wound models. In addition, this extract significant decreased level of malondialdehyde (MDA) in liver of treated-mice compared with that in non-treated mice. Conclusions Our results suggest that Piper betle Linn can be used as an ingredient in developing natural origin drugs for treatment of cutaneous wounds.

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In-vitro Investigation of Wound Healing Potential of Musa acuminata Leaf Extract

Analytical Chemistry Letters ◽

10.1080/22297928.2021.1892521 ◽

2021 ◽

Vol 11 (3) ◽

pp. 437-449

Author(s):

Ghosh Tanmoy ◽

Rajamanickam Deveswaran ◽

Sinhari Apurba ◽

K. Kavana ◽

R. Monisha ◽

...

Keyword(s):

Wound Healing ◽

Leaf Extract ◽

Musa Acuminata ◽

In Vitro Investigation

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Biogenic synthesis of silver nanoparticles using Persicaria odorata leaf extract: Antibacterial, cytocompatibility, and in vitro wound healing evaluation

Particuology ◽

10.1016/j.partic.2022.01.001 ◽

2022 ◽

Author(s):

Faridah Aminullah Lubis ◽

Nik Ahmad Nizam Nik Malek ◽

Nor Suriani Sani ◽

Khairunadwa Jemon

Keyword(s):

Wound Healing ◽

Silver Nanoparticles ◽

Leaf Extract ◽

Biogenic Synthesis

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EXAMINATION OF IN VITRO WOUND HEALING ANTIMICROBIAL ACTIVITY OF EXTRACT OF SELECTED MEDICINAL PLANTS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2020.v13i2.36475 ◽

2019 ◽

pp. 127-130

Author(s):

CHANDUKISHORE T ◽

SAMSKRATHI D ◽

SRUJANA TL

Keyword(s):

Wound Healing ◽

Medicinal Plants ◽

Leaf Extract ◽

Methanol Extract ◽

Maximum Yield ◽

Diffusion Method ◽

Zone Of Inhibition ◽

Solvent Extract ◽

Minimum Yield

Objective: The objective of this study was to discover and examine the in vitro wound healing activity of selected medicinal plants against common wound infecting microorganisms. Methods: Ziziphus rugosa and Hemidesmus indicus plant parts were used for aqueous and solvent extract preparation, maceration technique was followed. In vitro antibiotic test has been done using disc diffusion method. Results: Maximum yield for aqueous extract was observed in Z. rugosa bark sample (50.6%) and minimum yield was noted in H. indicus leaf extract in (45.7%) and maximum yield in solvent extract was observed in methanol extract of Z. rugosa (37.5%) whereas minimum yield was observed in of acetone extract of leaves of Z. rugosa (25%). For all sample extracts tested, leaf extract of acetone and methanol extract of Z. rugosa and H. indicus showed the highest antibiotic zone of inhibition of 15 mm and 12 mm, whereas lowest zone of inhibition was observed in aqueous bark extract of Z. rugosa (7 mm). Conclusion: Crude aqueous and solvent extract of selected plant were showed that promising results have a wound healing aid, efficacy of which could be further improved by studying and practicing more advanced extraction procedure for future prospectus.

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Mechanism of Wound-Healing Activity ofHippophae rhamnoidesL. Leaf Extract in Experimental Burns

Evidence-based Complementary and Alternative Medicine ◽

10.1093/ecam/nep189 ◽

2011 ◽

Vol 2011 ◽

pp. 1-9 ◽

Cited By ~ 51

Author(s):

Nitin K. Upadhyay ◽

Ratan Kumar ◽

M. S. Siddiqui ◽

Asheesh Gupta

Keyword(s):

Wound Healing ◽

Wound Repair ◽

Leaf Extract ◽

Lipid Peroxide ◽

Enzymatic Antioxidants ◽

Burn Wound ◽

Burn Wounds ◽

Aqueous Leaf Extract

The present investigation was undertaken to evaluate the healing efficacy of lyophilized aqueous leaf extract of Sea buckthorn (Hippophae rhamnoidesL., family Elaeagnaceae) (SBT) and to explore its possible mechanism of action on experimental burn wounds in rats. The SBT extract, at various concentrations, was applied topically, twice daily for 7 days. Treatment with silver sulfadiazine (SSD) ointment was used as reference control. The most effective concentration of the extract was found to be 5.0% (w/w) for burn wound healing and this was further used for detailed study. The SBT-treated group showed faster reduction in wound area in comparison with control and SSD-treated groups. The topical application of SBT increased collagen synthesis and stabilization at the wound site, as evidenced by increase in hydroxyproline, hexosamine levels and up-regulated expression of collagen type-III. The histological examinations and matrix metalloproteinases (MMP-2 and -9) expression also confirmed the healing efficacy of SBT leaf extract. Furthermore, there was significant increase in levels of endogenous enzymatic and non-enzymatic antioxidants and decrease in lipid peroxide levels in SBT-treated burn wound granulation tissue. The SBT also promoted angiogenesis as evidenced by anin vitrochick chorioallantoic membrane model andin vivoup-regulated vascular endothelial growth factor (VEGF) expression. The SBT leaf extract had no cytotoxic effect on BHK-21 cell line. In conclusion, SBT aqueous leaf extract possesses significant healing potential in burn wounds and has a positive influence on the different phases of wound repair.

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