Linking rhizosphere bacterial diversity and soil fertility in tobacco plants under different soil types and cropping pattern in Tanzania: A pilot study

This study aims to determine the response of corn-sorghum cropping patterns on several environmentally friendly fertilization packages that can improve the quality of soil fertility streamline the use of inorganic fertilizers and improve crop yields on dry land. This research was conducted at Akar-Akar Village, Bayan District, North Lombok. The experimental design used was a Randomized Block Design, by testing five environmentally friendly fertilization packages on the corn-sorghum cropping pattern and repeated 4 times. The results showed that the response of maize-sorghum cropping patterns to the fertilizing package 60% lower than the recommended fertilizer dosage with the addition of 12 tons of manure per ha accompanied by mycorrhizal inoculation increasing soil fertility shown by increasing nutrient status of N, P, K and ingredients soil organic matter, increasing plant nutrient uptake (N, P, K and Ca), growth, crop yields and MA activity in the soil.

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Different Tillage Options and Residue Management for Improvingcrop Productivity and Soil Fertility in Wheat-Maize-Rice Cropping Pattern

Bangladesh Agronomy Journal ◽

10.3329/baj.v22i2.47620 ◽

2020 ◽

Vol 22 (2) ◽

pp. 55-66

Author(s):

MI Hossain ◽

MA Ohab ◽

MHR Sheikh ◽

BL Nag

Keyword(s):

Soil Fertility ◽

Crop Residue ◽

Conventional Tillage ◽

Residue Management ◽

Research Centre ◽

Cropping Pattern ◽

Tillage Systems ◽

Strip Tillage ◽

Planting System ◽

Residue Retention

A three yearsfield experiment was conducted at Regional Wheat Research Centre, Shyampur, Rajshahiduring 2014-15 to 2017-18 with an objective to observe the effects on soil fertility and performance of the crops under different tillage and residue management for rice-wheat (RW) systems by adding a third pre-rice crop of maize. The experiment was conducted in split plot design with three replication. The tillage options viz. (i) Strip tillage (ST) (ii) Permanent bed (PB) and (iii) conventional (CT) tillage; two crop residue management, viz. (i) 0%=no residue and (ii) 30% residue retention were studied. The results indicated that keeping 30% crop residue in the field with minimum disturbance of soil had significant contribution on grain yield of wheat-maize-rice sequence compare to conventional practice of well-till without crop residue retention.The permanent bed planting system gave the highest yields of wheat (4.37 tha-1), maize (7.31 tha-1) and rice (4.40 tha-1) and followed by strip tillage and lowest in conventional tillage. Among the residue management, 30% residue retention showed the highest yields of wheat (4.46tha- 1), maize (7.39 tha-1) and rice (4.69 tha-1). Considering economic performance of all tillage systems, the permanent bed planting system performed the best among all other tillage options and followed by strip tillage. Contrarily, 30% residue retention gave the highest yield and increased 0.12-0.14% organic matter into the soil with more productive.The results indicates that, both tillage systems coupled with 30% residue retention might be a good option for higher yield as well as soil fertility for Wheat-Maize- Taman rice cropping pattern in drought prone areas of Bangladesh. Bangladesh Agron. J. 2019, 22(2): 55-66

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Identification & correlation of bacterial diversity in oral cancer and long-term tobacco chewers- A case-control pilot study

Journal of Medical Microbiology ◽

10.1099/jmm.0.001417 ◽

2021 ◽

Vol 70 (9) ◽

Author(s):

Shriya Sawant ◽

Jinesh Dugad ◽

Deepak Parikh ◽

Sathiyaraj Srinivasan ◽

Harinder Singh

Keyword(s):

Pilot Study ◽

Oral Cancer ◽

Bacterial Diversity ◽

Beta Diversity ◽

Oral Microbiome ◽

Content Type ◽

Oral Rinse ◽

Link Type ◽

Tobacco Chewing

Introduction. Squamous cell carcinoma is a highly aggressive type of oral cancer (OC). It is the most common cancer among men, and accounts for almost 90 % of all oral cancers in India. Consumption of tobacco is a leading factor contributing to maximum oral cancer incidences as per the WHO. Hypothesis/Gap statement. Researchers reported a direct association of microorganisms with dysbiosis in various oral lesions including oral cancer. However, there is a dearth of information related to compositional changes in the oral microbiome in long-term tobacco chewers and the Indian oral cancer population. Aim. The aim of this study was to identify and correlate the bacterial diversity in the oral cavity of tobacco chewers, patients with oral cancer and healthy subjects in the Indian population. Methods. Oral rinse samples were collected for ten subjects in each group followed by DNA extraction. The variable regions of the bacterial 16S rRNA gene (V6-V8) were amplified, sequenced, processed, and analysed using QIIME2 platform to assess alpha and beta diversity between the study groups. Results. This pilot study showed genus Streptococcus dominated the control group (18.54 %), and the abundance decreased in tobacco and OC group (9.63 and 5.45% respectively); whereas genus Prevotella dominated the tobacco and OC group (21.01 and 26.03% respectively). A shift in abundance of microbiome was observed from control population to oral cancer via the tobacco chewing population. Maximum alpha diversity of oral microbiome was found in Indian tobacco chewers. Beta diversity of tobacco chewers was similar to both the healthy population as well as oral cancer patients suggesting transitioning of the oral microbiome from healthy to oral cancer microbiome via the tobacco chewers microbiome. Conclusion. The data provides evidence of oral bacterial dysbiosis due to tobacco chewing habits that can further lead to progression towards cancer.

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Pilot study reveals lower bacterial diversity in reused Eastern Bluebird nests

BIOS ◽

10.1893/0005-3155-90.1.47 ◽

2019 ◽

Vol 90 (1) ◽

pp. 47

Author(s):

Corien Bakermans ◽

Sha'land Abbott ◽

Samantha Gorman ◽

Marja H. Bakermans

Keyword(s):

Pilot Study ◽

Bacterial Diversity ◽

Eastern Bluebird

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Response of soil biological properties and bacterial diversity to different levels of nitrogen application in sugarcane fields

AMB Express ◽

10.1186/s13568-021-01331-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Shangdong Yang ◽

Jian Xiao ◽

Tian Liang ◽

Weizhong He ◽

Hongwei Tan

Keyword(s):

Soil Fertility ◽

Bacterial Diversity ◽

Microbial Biomass Carbon ◽

Nitrogen Application ◽

Soil Bacterial Diversity ◽

N Application ◽

Sugarcane Production ◽

Bacterial Richness ◽

Soil Bacterial ◽

Dominant Bacteria

AbstractTo select an eco-friendly nitrogen (N) application level for sugarcane production, soil fertility and soil bacterial diversity under different nitrogen application levels were analyzed. Four levels of urea applications were high Nitrogen (H, 964 kg ha−1), medium Nitrogen (M, 482 kg ha−1), low Nitrogen (L, 96 kg ha−1) and no Nitrogen (CK, 0 kg ha−1) treatments, respectively. The results showed that the soil microbial biomass carbon and phosphorus were altered significantly by CK and L treatments. Moreover, the indexes of soil bacterial richness and diversity in the sugarcane field could be significantly improved by L. At the genus level, SC-I-84, Mycobacterium, Micropepsaceae, Saccharimonadales, Subgroup_2 and Acetobacteraceae were the unique dominant bacteria in the soil with the H treatment. JG30-KF-CM45 and Jatrophihabitans were the unique dominant genera in the M treatment. Subgroup_6, HSB_OF53-F07, Streptomyces, 67–14, SBR1031 and KD4-96 were the unique dominant genera in the L treatment. In contrast, FCPS473, Actinospica, 1921–2, Sinomonas, and Ktedonobacteraceae were the unique dominant genera in the CK treatment. The findings suggest that soil fertility all could be changed by different N application levels, but the most increasing integral effect only could be found in L. Moreover, even though soil bacterial richness could be significantly promoted by the M and H treatments, but soil bacterial diversity could not be significantly improved. On the contrary, soil bacterial diversity and richness all could be improved by L treatment. In addition, higher abundance of unique soil dominant bacteria could be only found in L treatment which compared to the CK, M and H treatments. These findings suggest that the rate of 96 kg ha−1 N application is ecofriendly for sugarcane production in Guangxi.

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Prediction of soil fertility considering their macronutrient status

Fundamental and Applied Soil Science ◽

10.15421/041711 ◽

2017 ◽

Vol 18 (3-4) ◽

pp. 5-20

Author(s):

V. L. Samokhvalova ◽

A. O. Khristenko ◽

L. O. Shedey ◽

P. A. Samokhvalova ◽

O. V. Karatsuba

Keyword(s):

Heavy Metals ◽

Soil Fertility ◽

Nitrogen Content ◽

Total Nitrogen ◽

Chemical Elements ◽

Total Nitrogen Content ◽

Soil Types ◽

Regression Equations ◽

Soil System ◽

Climatic Zones

The method for prediction of productive functions of soils, according to their macronutrient status, is grounded on the example of the total concentrations of nitrogen as biogenic macronutrient in different soil types of Polesie, Forest-Steppe and Steppe climatic zones of Ukraine including soils contaminated by heavy metals and fertilized (organic-mineral, organic and mineral system of fertilizer) soils. In the elaborated methodical approach the productive function (fertility) of different genesis soils forecasts by establishing new relationships of total nitrogen content with a humus calorific value, the energy reserves of the soil in layer up to 20 cm, the application of mathematical-statistical analysis. For example, according to the total nitrogen content as a biogenic macronutrient in accordance with the fixed gradations, with a further extension of the method algorithm for different soil types of climatic zones by the influence of technogenic pollution and technological load. The technical result of the elaborated method is to improve the known method of soil fertility prediction, considering their macronutrient status, by the selective choice of the most correlation associated, diagnostics capable of integral indicators of the energy and nitrogen status of the soil, which enhances the informativeness, accuracy and quick speed forecasting of production and ecological functions of different genesis soils with the identification of differences in ecological condition of soils for prediction and regulation of their quality. The elaborated methodological approach may find application in the assessment and ecological standardization of soil quality by chemical elements content, regulation of loads (technogenic, technological) on a soil system, in agroecology by investigating the question of soil fertility monitoring, lands certification of different purpose and use; organic farming, bioenergy and energy of soil formation; diagnosis, evaluation, determination of quality of humus and the state of the chemical elements; in environmental management of soils both for background conditions and different anthropogenic impacts and in research practice – investigating the biogeochemistry and nutrient macronutrients of the soil cover. Distinctive features and advantages of the proposed elaboration in comparison with known methods and approaches are: 1) express obtain of the accurate by predicted levels of total nitrogen content as a nutrient in the soil with the improving of the soil diagnosis accuracy by establishing some interconnected diagnostically suitable indicators of soils humus substances and nitrogen systems functioning, the direction of the mobilization and immobilization of nutrients (C, N) processes, the humification-mineralization and energy intensity of soils, including the negative impacts of anthropogenic pressures and degradation processes; 2) providing the opportunity for increasing effectiveness of predicting data on the functioning of the nitrogen systems of different genesis soils, environmental and energy state of soil and level of their potential fertility due to the background conditions, the application of different fertilization systems, and risk the availability of heavy metals pollution while minimizing the consumption of material resources; 3) expansion of the user’s ability to determine the total nitrogen content in the soil without long-term chemical analytical research due to the choice of regression equations obtained on the basis of the use of baseline soil properties of a certain type and subtype, according to available information; 4) versatility due to suitability of installed dependencies of the proposed method for all soil types and subtypes, climatic zones and contaminants.

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Diversity and Richness Analysis of the Oral and Gastrointestinal Microbiome during Autologous Transplantation for Multiple Myeloma: Results of a Prospective Pilot Study and Correlation with Transplant Outcomes

Blood ◽

10.1182/blood-2018-99-120038 ◽

2018 ◽

Vol 132 (Supplement 1) ◽

pp. 4627-4627

Author(s):

Najla H El Jurdi ◽

Ali Filali ◽

Iman Salem ◽

Mauricio Retuerto ◽

Nina Dambrosio ◽

...

Keyword(s):

Multiple Myeloma ◽

Pilot Study ◽

Microbial Diversity ◽

Bacterial Diversity ◽

Statistical Significance ◽

R Package ◽

Advisory Board ◽

Gastrointestinal Microbiome ◽

Fecal Samples ◽

Genus Richness

Abstract Background The human microbiome has been associated with allogeneic hematopoietic cell transplantation (HCT) outcomes, namely infections, graft-versus-host disease and relapse. There are no studies describing the longitudinal changes in the oral or gastrointestinal microbiome in the setting of autologous HCT. We conducted a prospective study to describe the changes in microbial diversity in patients undergoing HCT for multiple myeloma (MM), and whether these correlate with HCT outcomes and/or toxicities. Methods Samples were collected from 15 MM patients on admission (baseline, T-2), during marrow aplasia (T+7) and after engraftment (T+30) (Table 1- summarizes baseline characteristics). We evaluated the bacterial and fungal microbiome of 15 patients using Ion-Torrent PGM workflow. The amplicons generated from the 16s rRNA and the ITS genes were sequenced for bacterial and fungal identification, respectively. Sequencing reads were clustered into operational taxonomic units (OTUs, 3% distance) and taxonomically classified via Qiime bioinformatics pipeline. Diversity was calculated using Shannon diversity index and richness using the R package 'vegan'. Longitudinal analysis was performed using all pairwise Multiple Comparison of Mean Ranks as implemented PMCMR plus R package, employing Kruskal & Wallis test followed by Bonferroni-Dunn post-hoc adjustment. Results Diversity and richness of the oral mycobiome decreased at T+7 compared to pre-transplant levels with further decrease noted at T+30, without reaching significance. Fecal mycobiome diversity and richness decreased from baseline to T+7 meeting statistical significance for diversity (T-2 vs T+7, p=0.05) and richness trended towards significance (p=0.06) with a further decrease noted at T+30. The temporal changes in bacterial diversity and richness in both oral and fecal samples did not reach statistical significance. (Figure1- Box and whisker plots of diversity and richness of the bacteriome and mycobiome at the genus levels from oral rinse and fecal samples) In fecal samples, bacterial diversity noted at T+7 during count nadir was associated with the severity of diarrhea experienced after myeloablation, with lower diversity correlating with more severe diarrhea (p= 0.03). Anaerobic targeting antibiotic exposure on or before T+7 affected both the genus diversity and richness at T+7 (p=0.015 and p=0.014, respectively). The bacterial genus richness at baseline (p=0.03) as well as the diversity and richness noted at T+7 (p=0.01) was associated with the development of fever on or after T+7. For the oral mycobiome, exposure to anaerobic depleting antibiotics correlated with genus richness in T+30 samples (p=0.04). There was a trend towards significance between the diversity of fecal samples at baseline and the development of nausea post transplant, such that higher diversity was associated with lower incidence/severity of nausea (p=0.06). Conclusion and Future Directions While acknowledging the limitation inherent in the small sample size of this pilot study, our results highlight several aspects of the longitudinal changes in the microbiome during HCT. Oral and lower gastrointestinal microbial diversity and richness is altered during HCT with trends significantly different between the oral and fecal bacteriome and mycobiome. This change is likely multifactorial owing to the conditioning regimen, antimicrobial exposure and immune dysregulation. Our data suggest a possible correlation between exposure to anaerobic organism depleting antimicrobials and these changes in microbial diversity and richness at the genus level. Baseline microbial diversity and richness as well as changes coinciding with marrow aplasia could correlate with the incidence and severity of transplant related toxicities. Amifostine was used as a cytoprotectant before high dose melphalan for our patients. The effect of this organic thiophosphate on the microbiota is unclear and it would be of interest to compare matched patient samples to explore the effect of this cytoprotectant on the microbiota. Further studies conducted on a larger scale and incorporating metabolomics and proteomics will help elucidate the interactions between the host and the microbiome and their effect on short term and long term transplantation outcomes as well as toxicities. Disclosures Lazarus: Pluristem Ltd.: Consultancy. Caimi:Celgene: Speakers Bureau; Kite Pharma: Other: Advisory Board Participation; Kite Pharma: Other: Advisory Board Participation; Genentech: Other: Advisory Board PArticipation, Research Funding. Malek:Janssen: Consultancy, Speakers Bureau; Amgen: Consultancy, Speakers Bureau; Takeda: Consultancy, Speakers Bureau; Celgene: Consultancy, Speakers Bureau; Sanofi: Consultancy, Speakers Bureau.

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