Biological synthesis of gold and silver nanoparticles using leaf extracts of Crassocephalum rubens and their comparative in vitro antioxidant activities

Silver nanoparticles are widely used in the biomedical and agri-food fields due to their versatility. The use of biological methods for the synthesis of silver nanoparticles has increased considerably due to their feasibility and high biocompatibility. In general, microorganisms have been widely explored for the production of silver nanoparticles for several applications. The objective of this work was to evaluate the use of entomopathogenic fungi for the biological synthesis of silver nanoparticles, in comparison to the use of other filamentous fungi, and the possibility of using these nanoparticles as antimicrobial agents and for the control of insect pests. In addition, the in vitro methods commonly used to assess the toxicity of these materials are discussed. Several species of filamentous fungi are known to have the ability to form silver nanoparticles, but few studies have been conducted on the potential of entomopathogenic fungi to produce these materials. The investigation of the toxicity of silver nanoparticles is usually carried out in vitro through cytotoxicity/genotoxicity analyses, using well-established methodologies, such as MTT and comet assays, respectively. The use of silver nanoparticles obtained through entomopathogenic fungi against insects is mainly focused on mosquitoes that transmit diseases to humans, with satisfactory results regarding mortality estimates. Entomopathogenic fungi can be employed in the synthesis of silver nanoparticles for potential use in insect control, but there is a need to expand studies on toxicity so to enable their use also in insect control in agriculture.

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Larvicidal potential of gold and silver nanoparticles synthesized using Acalypha fruticosa leaf extracts against Culex pipiens (Culicidae: Diptera)

Journal of Asia-Pacific Entomology ◽

10.1016/j.aspen.2020.12.007 ◽

2021 ◽

Vol 24 (1) ◽

pp. 184-189

Author(s):

Sadeq K. Alhag ◽

Fahd A. Al-Mekhlafi ◽

Nael Abutaha ◽

Fahd Mohammed Abd Al Galil ◽

Muhammad A. Wadaan

Keyword(s):

Silver Nanoparticles ◽

Culex Pipiens ◽

Leaf Extracts ◽

Gold And Silver Nanoparticles

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FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2018.v10s1.90 ◽

2018 ◽

Vol 10 (1) ◽

pp. 407

Author(s):

Dewi Kumala Putri ◽

Berna Elya ◽

Nuraini Puspitasari

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Hexane Extract ◽

Phytochemical Screening ◽

Active Fraction ◽

Leaf Extracts ◽

Antioxidant Power ◽

Dpph Method ◽

Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

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Functionalized silver nanoparticles synthesized from marine algae: In vitro Evaluation of Antibacterial Potential

Research Journal of Biotechnology ◽

10.25303/168rjbt3821 ◽

2021 ◽

Vol 16 (8) ◽

pp. 38-49

Author(s):

Siva Kumar Kandula ◽

Satyanarayana Swamy Cheekatla ◽

Venkata Satya Mahesh Kumar Metta ◽

Venkata Rajagopal Saladi

Keyword(s):

Silver Nanoparticles ◽

Antioxidant Activities ◽

Algal Species ◽

Synthesis Method ◽

Radical Scavenging ◽

Visible Spectrum ◽

Total Phenolic ◽

Face Centered Cubic ◽

Frap Assay

Natural antioxidants, in particular phenolic derivatives, are used efficiently to combat against oxidative induced tissue damages. The objective of the study is to determine the antioxidant potential of methanolic extracts obtained from eight marine algal species (Enteromorpha compressa, Chaetomorpha antennina, Caulerpa racemosa, Caulerpa taxifolia, Sargassum vulgare, Padina tetrastromatica, Amphiroa fragilissima and Gracilaria corticata) by assessing their total phenolic content, DPPH scavenging assay, FRAP assay, H2O2 radical and superoxide radical scavenging activities. Among them, P.tetrastromatica, S.vulgare, E.compressa, C.taxifolia display significant antioxidant activities. Further, the aqueous extracts of these four algae are used for bioreduction of silver nitrate to silver nanoparticles (AgNPs) by green synthesis method at room temperature. UV-Visible spectrum revealed the surface plasmon resonance at 430 and 440nm. The characterizations of AgNPs by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) studies revealed the bioreduction and capping of AgNPs. XRD analysis elucidated the synthesized nanoparticles having face centered cubic crystalline geometry, with a mean size of 17 nm. The nanoparticles have better antimicrobial activity against cocci shaped than the rod shaped bacteria. The minimum inhibitory concentration and minimum bactericidal concentration exhibit more activity against S.aureus and B.cereus rather than E.coli.

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DETECTION OF BIOACTIVE FRACTIONS OF JUSTICIA ADHATODA L. LEAVES

Canadian Journal of Applied Sciences ◽

10.21065/19257430.388.3 ◽

2013 ◽

Vol 3 ◽

pp. 388

Author(s):

Faiza Rasheed ◽

Keyword(s):

Crude Oil ◽

Metal Ions ◽

Dietary Fiber ◽

Total Alkaloid ◽

Antioxidant Activities ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Essential Metal ◽

Justicia Adhatoda

In vitro antibacterial and antioxidant activities of various leaf extracts of Justicia adhatoda L. (locally known as Bhaikar) were assessed. The leaves were also subjected to various phytochemical analysis. Results revealed that leaves of J. adhatoda L. contain significant amount of total alkaloid, phenols flavonoid, saponins, tannins , protein, crude oil, dietary fiber, essential and non essential metal ions. The methanol, ethanol, butanol, chloroform and n-hexane leaf extracts of J

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In vitro antioxidant activity and phytochemical analyses of Acalypha godseffiana (Euphobiaceae) leaf extracts

Nigerian Journal of Natural Products and Medicine ◽

10.4314/njnpm.v25i1.8 ◽

2021 ◽

Vol 25 (1) ◽

pp. 75-79

Author(s):

S.O. Olubodun ◽

G.E. Eriyamremu ◽

M.E. Ayevbuomwan ◽

C.I. Nzoputa

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Antioxidant Vitamin ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Anti Oxidant ◽

In Vitro Antioxidant Activity

The presence of various bioactive components makes it necessary to analyse plants for their potential to act as a source of useful treatments and cures for many inflammatory, infectious and pathogenic diseases. This study was carried out to determine phytochemicals and in-vitro antioxidant activities of the leaf extracts of Acalypha godseffiana. The leaves of A. godseffiana were collected, dried, pulverized and extracted separately with methanol and water using maceration method. The extract was concentrated in vacuo with rotary evaporator at 40oC. The extracts were subjected to quantitative phytochemical analysis and different anti-oxidant analytical procedures like FRAP, DPPH etc to determine the radical scavenging capabilities. The results of phytochemical analysis estimated the quantities and revealed the presence of alkaloids, flavonoids, tannins, saponins and terpenoids which varied in both extracts. The methanol and aqueous extracts exhibited antioxidant activities with relatively high IC50 (IC50 = 3.67 ìg/ml and 4.42ìg/ml respectively) which accounted for a low free radical-scavenging activity when compared with the reference antioxidant, vitamin C (IC50 = 1.51ìg/ml). The results of the study indicates that A. godseffiana leaf extracts contain secondary metabolites and possesses antioxidant properties.

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Functional Components of Bamboo Shavings and Bamboo Leaf Extracts and Their Antioxidant Activities In Vitro

Journal of Medicinal Food ◽

10.1089/jmf.2014.3189 ◽

2015 ◽

Vol 18 (4) ◽

pp. 453-459 ◽

Cited By ~ 23

Author(s):

Jinyan Gong ◽

Daozong Xia ◽

Jun Huang ◽

Qing Ge ◽

Jianwei Mao ◽

...

Keyword(s):

Antioxidant Activities ◽

Leaf Extracts ◽

Functional Components

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Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Essential Oils from AgNPs and AuNPs Elicited Lavandula angustifolia In Vitro Cultures

Molecules ◽

10.3390/molecules24030606 ◽

2019 ◽

Vol 24 (3) ◽

pp. 606 ◽

Cited By ~ 7

Author(s):

Aneta Wesołowska ◽

Paula Jadczak ◽

Danuta Kulpa ◽

Włodzimierz Przewodowski

Keyword(s):

Molecular Weight ◽

Silver Nanoparticles ◽

Essential Oils ◽

Culture Media ◽

In Vitro Cultures ◽

Gas Chromatography Mass Spectrometry ◽

Lavandula Angustifolia ◽

Gold And Silver Nanoparticles ◽

The Media

The aim of this study was to determine how the addition of gold and silver nanoparticles to culture media affects the composition of essential oils extracted from Lavandula angustifolia propagated on MS media with the addition of 10 and 50 mg·dm−3 of gold (24.2 ± 2.4 nm) and silver (27.5 ± 4.8 nm) nanocolloids. The oil extracted from the lavender tissues propagated on the medium with 10 mg·dm−3 AgNPs (silver nanoparticles) differed the most with respect to the control; oil-10 compounds were not found at all, and 13 others were detected which were not present in the control oil. The addition of AuNPs (gold nanoparticles) and AgNPs to the media resulted in a decrease of lower molecular weight compounds (e.g., α- and β-pinene, camphene, δ-3-carene, p-cymene, 1,8-cineole, trans-pinocarveol, camphoriborneol), which were replaced by those of a higher molecular weight (τ- and α-cadinol 9-cedranone, cadalene, α-bisabolol, cis-14-nor-muurol-5-en-4-one, (E,E)-farnesol).

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