scholarly journals Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Essential Oils from AgNPs and AuNPs Elicited Lavandula angustifolia In Vitro Cultures

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 606 ◽  
Author(s):  
Aneta Wesołowska ◽  
Paula Jadczak ◽  
Danuta Kulpa ◽  
Włodzimierz Przewodowski
Keyword(s):  
Molecular Weight ◽  
Silver Nanoparticles ◽  
Essential Oils ◽  
Culture Media ◽  
In Vitro Cultures ◽  
Gas Chromatography Mass Spectrometry ◽  
Lavandula Angustifolia ◽  
Gold And Silver Nanoparticles ◽  
The Media

The aim of this study was to determine how the addition of gold and silver nanoparticles to culture media affects the composition of essential oils extracted from Lavandula angustifolia propagated on MS media with the addition of 10 and 50 mg·dm−3 of gold (24.2 ± 2.4 nm) and silver (27.5 ± 4.8 nm) nanocolloids. The oil extracted from the lavender tissues propagated on the medium with 10 mg·dm−3 AgNPs (silver nanoparticles) differed the most with respect to the control; oil-10 compounds were not found at all, and 13 others were detected which were not present in the control oil. The addition of AuNPs (gold nanoparticles) and AgNPs to the media resulted in a decrease of lower molecular weight compounds (e.g., α- and β-pinene, camphene, δ-3-carene, p-cymene, 1,8-cineole, trans-pinocarveol, camphoriborneol), which were replaced by those of a higher molecular weight (τ- and α-cadinol 9-cedranone, cadalene, α-bisabolol, cis-14-nor-muurol-5-en-4-one, (E,E)-farnesol).

scholarly journals APLIKASI SITOKININ TIPE PURIN DAN UREA PADA MULTIPLIKASI TUNAS ANIS (Pimpinellla anisum L.) IN VITRO

2020 ◽  
Vol 13 (1) ◽  
pp. 1
Author(s):  
OTIH ROSTIANA
Keyword(s):  
Essential Oils ◽  
Culture Media ◽  
Shoot Multiplication ◽  
Tropical Region ◽  
Herbaceous Plant ◽  
Solid Culture ◽  
Pimpinella Anisum ◽  
The Media ◽  
Initiation Stage

ABSTRAK<br />Anis (Pimpinella anisum L.) merupakan tanaman herba tahunan<br />yang termasuk ke dalam famili Umbelliferae. Buahnya diketahui<br />mengandung minyak atsiri yang didominasi senyawa trans-anethol (90%)<br />dan berkhasiat sebagai antiseptik, antispasmodik, antikanker, karminatif,<br />pelega tenggorokan, obat bronkitis, serta digunakan dalam pembuatan<br />sabun, parfum, pasta gigi, juga krim kulit. Sebagai tanaman bernilai<br />ekonomi, upaya perbanyakan anis perlu dilakukan. Perbanyakan secara in<br />vitro dengan teknik kultur jaringan merupakan salah satu metode alternatif<br />yang dapat digunakan untuk menghasilkan bibit dalam jumlah banyak,<br />seragam dan dalam waktu yang relatif singkat. Dengan penambahan<br />sitokinin sintetik tipe urea seperti thidiazuron (TDZ) dan tipe purin seperti<br />benzil amino purin (BAP) akan memacu inisiasi dan proliferasi tunas.<br />Penelitian ini bertujuan mendapatkan media yang tepat untuk menginduksi<br />tunas anis yang optimal dengan penambahan BAP atau TDZ, mengetahui<br />respon pertumbuhan dan penampakan kultur akibat penambahan berbagai<br />konsentrasi BAP atau TDZ, serta mempelajari sinergisme yang terjadi<br />antara keduanya. Pada tahap inisiasi, eksplan berupa tunas pucuk diinduksi<br />di dalam media MS padat dengan penambahan BAP (0,1 mg/l; 0,2 mg/l;<br />0,3 mg/l; 1 mg/l; 2 mg/l; 3 mg/l), atau TDZ dengan kisaran konsentrasi<br />yang sama. Tunas terbanyak yang dihasilkan dari dua jenis sitokinin pada<br />tahap ini disubkultur ke dalam media yang ditambahkan jenis sitokinin<br />yang berbeda (TDZ ke BAP atau BAP ke TDZ) pada konsentrasi 0,3 mg/l<br />atau 3 mg/l. Pada media yang ditambahkan TDZ dihasilkan tunas anis<br />lebih banyak (3,62-6,28) dibandingkan pada media yang ditambahkan<br />BAP (1,86-2,78), tetapi tunas yang dihasilkan pendek (roset). Sedangkan<br />tunas yang dihasilkan dalam media yang ditambahkan BAP beruas lebih<br />tinggi tetapi jumlah tunasnya sedikit. Subkultur tunas anis ke dalam media<br />yang diperkaya dengan sitokinin yang berbeda meningkatkan jumlah tunas<br />yang berproliferasi dan memperbaiki visual tunas.<br />Kata kunci: Anis, Pimpinellla anisum L. ,  minyak atsiri, multiplikasi tunas,<br />in vitro, TDZ, BAP, Jawa Barat<br />ABSTRACT<br />Application of purine and urea types of cytokinins in<br />shoot multiplication of Anise (Pimpinella anisum L.) in<br />vitro<br />Pimpinella anisum L. or sweet anise is an annual–herbaceous plant<br />belongs to the Umbelliferae family. The fruit of anise contains of essential<br />oil, which is mainly consisted of trans-anethol (90%). Essential oils of<br />anise is mainly used as an antiseptic, antispasmodic, anticancer,<br />carminative, expectorant and has also been used as component in soap,<br />perfumery, tooth paste, and skin cream productions. Since this crop is<br />mainly cultivated in sub tropical region, anise cultivation in Indonesia has<br />not been performed. To obtain sufficient numbers of anise planting<br />materials in vitro propagation was conducted by applying benzyl amino<br />purine (BAP) and thidiazuron (TDZ). In this research TDZ or BAP were<br />applied at various concentrations (0,1 mg/l: 0.2 mg/l; 0.3 mg/l; 1 mg/l; 2<br />mg/l; 3 mg/l), to induce shoots in MS-solid culture media. The highest<br />number of shoots obtained in those two type of cytokinins containing<br />media from the initiation stage were subcultured into the media<br />supplemented with different cytokinins (TDZ to BAP or BAP to TDZ) at<br />0.3 mg/l or 3 mg/l levels. The results showed that medium with the<br />addition of TDZ resulted in higher numbers of shoot (3,26-6,28) than that<br />of medium with an addition of BAP (1,86-2,78). However, rosette shoots<br />were dominant in TDZ containing medium. On the other hand, medium<br />with an addition of BAP resulted in less numbers of shoots with taller<br />nodes. Subculture of anise into different kinds of cytokinins increased the<br />numbers of proliferated-shoots and recovered the abnormal shoots.<br />Key words : Anise, Pimpinellla anisum L, essential oils, shoots<br />multiplication, in vitro, TDZ, BAP, West Java

scholarly journals Pleurotus spp. Mycelia Enriched in Magnesium and Zinc Salts as a Potential Functional Food

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 162
Author(s):  
Anna Włodarczyk ◽  
Agata Krakowska ◽  
Katarzyna Sułkowska-Ziaja ◽  
Małgorzata Suchanek ◽  
Piotr Zięba ◽  
...  
Keyword(s):  
Organic Compounds ◽  
Functional Food ◽  
Functional Foods ◽  
Culture Media ◽  
In Vitro Cultures ◽  
Immunostimulatory Activity ◽  
Zinc Salts ◽  
Health Promoting ◽  
The Media

Worldwide, mushrooms belonging to the Pleurotus spp. such as P. citrinopileatus, P. djamor, and P. pulmonarius are highly valued not only for their taste and aroma but also for their health-promoting properties. These species are rich in bioelements, vitamins, and above all, compounds that exhibit immunostimulatory activity. Therefore, in this study, we aimed to determine the effect of the supplementation of culture media using inorganic Mg and Zn salts. This is the first study to establish the bioavailability of the selected elements (Mg and Zn) and anions (Cl−, SO42−) from the enriched biomass by means of the extraction of lyophilized mycelium into artificial digestive juices. The following salts were added to the liquid Oddoux medium: ZnSO4·7H2O, ZnCl2, MgSO4·7H2O or MgCl2·6H2O. The bioelements, anions and organic compounds in the obtained biomass were determined. The addition of Zn and Mg salts to the media increased the production of biomass by 30% and increased the bioaccumulation of the inorganic salts. Maintaining in vitro cultures under optimized and controlled conditions produced mycelium with a better composition and health properties than otherwise. Such enriched biomass may be classified as potential functional foods, aiding in overcoming deficiencies of elements and organic compounds with biological activity in humans.

Biochemical markers for pregnancy in the spent culture medium of in vitro produced bovine embryos

2021 ◽  
Author(s):  
Gabriela de Oliveira Fernandes ◽  
Marcella Pecora Milazzotto ◽  
Andrei Antonioni Guedes Fidelis ◽  
Taynan Stonoga Kawamoto ◽  
Ligiane de Oliveira Leme ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Biochemical Markers ◽  
Culture Medium ◽  
Culture Media ◽  
Ionization Mass ◽  
Metabolic Profiles ◽  
Bovine Embryos ◽  
The Media

Abstract The present study aimed to identify biomarkers to assess the quality of in vitro produced (IVP) bovine embryos in the culture media. IVP embryos on Day (D) 5 of development were transferred to individual drops, where they were maintained for the last 48 h of culture. Thereafter, the medium was collected and the embryos were transferred to the recipients. After pregnancy diagnosis, the media were grouped into the pregnant and nonpregnant groups. The metabolic profiles of the media were analyzed via electrospray ionization mass spectrometry, and the concentrations of pyruvate, lactate, and glutamate were assessed using fluorimetry. The spectrometric profile revealed that the media from embryos from the pregnant group presented a higher signal intensity compared to that of the nonpregnant group; the ions 156.13 Da [M + H]+, 444.33 Da [M + H]+, and 305.97 Da [M + H]+ were identified as biomarkers. Spent culture medium from expanded blastocysts (Bx) that established pregnancy had a greater concentration of pyruvate (p = 0.0174) and lesser concentration of lactate (p = 0.042) than spent culture medium from Bx that did not establish pregnancy. Moreover, pyruvate in the culture media of Bx can predict pregnancy with 90.9% sensitivity and 75% specificity. In conclusion, we identified markers in the culture media that helped in assessing the most viable IVP embryos with a greater potential to establish pregnancy.

scholarly journals Phytotoxicity of Essential Oils on Selected Weeds: Potential Hazard on Food Crops

Plants ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 79 ◽  
Author(s):  
María Ibáñez ◽  
María Blázquez
Keyword(s):  
Seed Germination ◽  
Essential Oil ◽  
Essential Oils ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Food Crops ◽  
Potential Hazard ◽  
Spectrometry Analysis

The chemical composition of winter savory, peppermint, and anise essential oils, and in vitro and in vivo phytotoxic activity against weeds (Portulaca oleracea, Lolium multiflorum, and Echinochloa crus-galli) and food crops (maize, rice, and tomato), have been studied. Sixty-four compounds accounting for between 97.67–99.66% of the total essential oils were identified by Gas Chromatography-Mass Spectrometry analysis. Winter savory with carvacrol (43.34%) and thymol (23.20%) as the main compounds produced a total inhibitory effect against the seed germination of tested weed. Menthol (48.23%), menthone (23.33%), and iso-menthone (16.33%) from peppermint only showed total seed germination inhibition on L. multiflorum, whereas no significant effects were observed with trans-anethole (99.46%) from anise at all concentrations (0.125–1 µL/mL). Low doses of peppermint essential oil could be used as a sustainable alternative to synthetic agrochemicals to control L. multiflorum. The results corroborate that in vivo assays with a commercial emulsifiable concentrate need higher doses of the essential oils to reproduce previous in vitro trials. The higher in vivo phytotoxicity of winter savory essential oil constitutes an eco-friendly and less pernicious alternative to weed control. It is possible to achieve a greater in vivo phytotoxicity if less active essential oil like peppermint is included with other active excipients.

scholarly journals Chemical Profile, In Vitro Biological Activity and Comparison of Essential Oils from Fresh and Dried Flowers of Lavandula angustifolia L.

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5317
Author(s):  
Claudio Caprari ◽  
Francesca Fantasma ◽  
Fabio Divino ◽  
Antonio Bucci ◽  
Maria Iorizzi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Fruits And Vegetables ◽  
Central Italy ◽  
Radical Scavenging ◽  
Sclerotium Rolfsii ◽  
Bactericidal Effect ◽  
Phytopathogenic Fungus ◽  
Lavandula Angustifolia

The chemical composition of essential oils (EOs) from dried and fresh flowers of Lavandula angustifolia L. (lavender), named LA 2019 and LA 2020, respectively, grown in central Italy was analyzed and compared by GC and GC-MS. For both samples, 61 compounds were identified, corresponding to 97.9% and 98.1% of the total essential oils. Explorative data analysis, performed to compare the statistical composition of the samples, resulted in a high level of global similarity (around 93%). The compositions of both samples were characterized by 10 major compounds, with a predominance of Linalool (35.3–36.0%), Borneol (15.6–19.4%) and 1,8-Cineole (11.0–9.0%). The in vitro antibacterial activity assay by disk diffusion tests against Bacillus subtilis PY79 and Escherichia coli DH5α showed inhibition of growth in both indicator strains. In addition, plate counts revealed a bactericidal effect on E. coli, which was particularly noticeable when using oil from the fresh lavender flowers at the highest concentrations. An in vitro antifungal assay showed that the EOs inhibited the growth of Sclerotium rolfsii, a phytopathogenic fungus that causes post-harvest diseases in many fruits and vegetables. The antioxidant activity was also assessed using the ABTS free radical scavenging assay, which showed a different antioxidant activity in both EOs. In addition, the potential application of EOs as a green method to control biodeterioration phenomena on an artistic wood painting (XIX century) was evaluated.

scholarly journals Effect of Jasmonic Acid and Salicylic Acid on Growth and Biochemical Composition of In-Vitro-Propagated Lavandula angustifolia Mill

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1722
Author(s):  
Ileana Miclea ◽  
Andreea Suhani ◽  
Marius Zahan ◽  
Andrea Bunea
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Plant Material ◽  
Culture Media ◽  
Carotenoid Content ◽  
Lavandula Angustifolia ◽  
Beneficial Impact ◽  
Free Media

This study assessed the effect of jasmonic acid (JA) and salicylic acid (SA) on the in vitro development and production of Lavandula angustifolia Mill. plant material, and the accumulation of polyphenols, chlorophylls, and carotenoids in explants. Results were compared with explants grown in control media and with in-vivo-grown mature and young L. angustifolia plants. After 21 days of incubation, all explants propagated on low-SA-concentration or elicitor-free media produced a greater number of shoots than explants cultivated on media with higher elicitor concentrations. Shoots grew taller when activated charcoal (AC) was added to the elicitor-supplemented media, while AC negatively affected or had no effect on the phytochemical composition of plants. Explants grown in the presence of elicitors had higher polyphenolic and chlorophyll content than the controls, demonstrating the beneficial impact of elicitors on the secretion of secondary metabolites. Lutein and β-carotene were the dominating carotenoids in all samples. Culture media supplemented with 0.5 mg/L JA and 1.5 mg/L SA + AC proved the most suitable to produce plant material with high polyphenol and carotenoid content, comparable with in-vivo-grown plants.

scholarly journals Chemical Composition and Biological Activities of Essential Oils of Pinus patula

2011 ◽  
Vol 6 (10) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Ismail Amri ◽  
Hamrouni Lamia ◽  
Samia Gargouri ◽  
Mohsen Hanana ◽  
Mariem Mahfoudhi ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Biological Activities ◽  
Pathogenic Fungi ◽  
Plant Diseases ◽  
Gas Chromatography Mass Spectrometry ◽  
Pinus Patula ◽  
Flame Ionization ◽  
Total Oil

Essential oils isolated from needles of Pinus patula by hydrodistillation were analyzed by gas chromatography-flame ionization detection (GC-FID) and gas chromatography mass spectrometry (GC-MS). Thirty-eight compounds were identified, representing 98.3% of the total oil. The oil was rich in monoterpene hydrocarbons (62.4%), particularly α-pinene (35.2%) and β-phellandrene (19.5%). The in vitro antifungal assay showed that P. patula oil significantly inhibited the growth of 9 plant pathogenic fungi. The oil, when tested on Sinapis arvensis, Lolium rigidum, Phalaris canariensis and Trifolium campestre, completely inhibited seed germination and seedling growth of all species. Our preliminary results showed that P. patula essential oil could be valorized for the control of weeds and fungal plant diseases.

scholarly journals In vitro biological assessment of the stability of cigarette smoke aqueous aerosol extracts

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Mark Taylor ◽  
Simone Santopietro ◽  
Andrew Baxter ◽  
Nicole East ◽  
Damien Breheny ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cigarette Smoke ◽  
Culture Media ◽  
Physical Stability ◽  
Bronchial Epithelial Cell ◽  
Biological Assessment ◽  
Gas Chromatography Mass Spectrometry ◽  
Fresh Sample ◽  
The Stability

Abstract Objectives Cigarette smoke aqueous aerosol extracts (AqE) have been used for assessing tobacco products, particularly with in vitro models such as oxidative stress and inflammation. These test articles can be generated easily, but there are no standardised methods for the generation and characterisation or stability. We investigated the effects of pro-oxidant smoke-derived chemicals by using 3R4F AqE generated under standardised conditioning and smoking regimes and assessed the stability over 31-week timeframe. Twenty batches generated from ten puffs per cigarette bubbled through 20 ml cell culture media were used fresh and thawed from frozen aliquots stored at – 80 ºC. Results Nicotine levels quantified by gas chromatography/mass spectrometry and optical density at 260 nm showed chemical and physical stability from week 0 (fresh sample) to weeks 1, 4, 8 and 31 (frozen samples). No significant change in H292 human bronchial epithelial cell viability or oxidative stress were observed between fresh AqE at week 0 and frozen AqE at 31 weeks. AqEs generated by our protocol were stable for up to 31 weeks for all tested end points, suggesting that it may not be necessary to use freshly generated AqE for each study, thus reducing batch-to-batch variability.

scholarly journals Potential Application of Prunus armeniaca L. and P. domestica L. Leaf Essential Oils as Antioxidant and of Cholinesterases Inhibitors

Antioxidants ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 2 ◽  
Author(s):  
Marco Bonesi ◽  
Maria Tenuta ◽  
Monica Loizzo ◽  
Vincenzo Sicari ◽  
Rosa Tundis
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Antioxidant Properties ◽  
Positive Control ◽  
Prunus Armeniaca ◽  
Gas Chromatography Mass Spectrometry ◽  
Flame Ionization ◽  
Ic50 Values ◽  
Comprehensive Comparison

The aim of this work is to investigate the in vitro acetylcholinesterase (AChE) and butyrycholinesterase (BChE) inhibitory activities of essential oils obtained by hydrodistillation from the leaves of Prunus armeniaca and P. domestica in relation to their composition, analysed by Gas Chromatography–Flame Ionization Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS) analyses, at different times. Moreover, considering the role of free radicals in the progression of neurodegenerative disorders, the antioxidant properties of essential oils were investigated by using, 2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and β-carotene bleaching tests. The relative antioxidant capacity index (RACI) was used to achieve more comprehensive comparison between analysed antioxidant effects of essential oils. P. armeniaca oils were more active than P. domestica oils against AChE. Against BChE, the most active was the essential oil from P. domestica leaves collected in August with an IC50 value of 95.80 μg/mL. This oil exerted the highest inhibitory activity of lipid peroxidation with IC50 values of 11.15 and 11.39 μg/mL after 30 and 60 min of incubation, respectively. All samples demonstrated a remarkable ABTS radicals scavenging activity, with IC50 values in the range 0.45–0.57 μg/mL in comparison to the positive control, ascorbic acid.

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