Introduction:
Escherichia coli is one of the most important agents involved in
healthcare-associated infection, and resistance to quantum ammonium compounds (QACs) has
become a major challenge for infection control practitioners. The aim of the current study was to
determine the frequency of qacE and qacEΔ1 genes in E. coli isolated from hospitalized patients
in Qazvin, Iran.
Material and Methods:
In the current cross-sectional study, 102 E. coli were collected from hospitals
of Qazvin. All bacterial isolates were identified using standard laboratory methods and the
antimicrobial susceptibility was evaluated by Kirby-Baer test. The presence of qacE and qacEΔ1
genes was investigated using polymerase chain reaction (PCR) technique.
Results:
In this study, 65 (63.7%) isolates showed a multidrug resistance (MDR) pattern which
was resistant to at least three classes of antimicrobials including ß-lactams, aminoglycosides, and
fluoroquinolones. The highest rates of resistance were observed against cefotaxime (75.5%) and
nalidixic acid (66.7%). The PCR showed that 5 (4.9%) isolates harbored qacE gene, 62 (60.8%)
isolates qacEΔ1, and 10 (9.8%) isolates carried both genes, simultaneously. There was a significant
relationship between the QACs resistance and MDR pattern (P=0.03).
Conclusion:
This study indicated a significant resistance rate against disinfectant compounds in
the studied hospitals. However, more attention should be paid to this critical issue in the infection
control committees of the hospitals.