scholarly journals Emergence of extended-spectrum β-lactam resistance among Escherichia coli at a US academic children's hospital is clonal at the sequence type level for CTX-M-15, but not for CMY-2

2013 ◽  
Vol 41 (5) ◽  
pp. 414-420 ◽  
Author(s):  
Scott J. Weissman ◽  
Amanda Adler ◽  
Xuan Qin ◽  
Danielle M. Zerr
Keyword(s):  
Escherichia Coli ◽  
Sequence Type ◽  
Children's Hospital ◽  
Extended Spectrum ◽  
Children’S Hospital

scholarly journals Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella Species in Pediatric Patients Visiting International Friendship Children’s Hospital, Kathmandu, Nepal

2020 ◽  
Vol 13 ◽  
pp. 117863372090979 ◽  
Author(s):  
Karuna Kayastha ◽  
Binod Dhungel ◽  
Shovana Karki ◽  
Bipin Adhikari ◽  
Megha Raj Banjara ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pediatric Patients ◽  
Diffusion Method ◽  
Esbl Production ◽  
Klebsiella Species ◽  
Children's Hospital ◽  
Extended Spectrum ◽  
Routine Identification ◽  
Children’S Hospital ◽  
Esbl Producers

Introduction: Emergence and spread of antimicrobial resistance (AMR) is a global threat and significantly affects the treatment options for common infectious diseases. Inappropriate use of antibiotics, particularly third-generation cephalosporins, has contributed to the development of AMR. This study aims to determine the prevalence of extended-spectrum β-lactamase (ESBL) production in Escherichia coli and Klebsiella species isolated from various clinical samples. Methods: This cross-sectional study was conducted at International Friendship Children’s Hospital, Kathmandu, Nepal, from August 2017 to January 2018. A total of 1443 samples that included urine, pus, wound swab, endotracheal tip, catheter tip, and blood were collected from pediatric patients below 15 years and processed by standard microbiological methods. Following sufficient incubation, isolates were identified by colony morphology, gram staining, and necessary biochemical tests. Identified bacterial isolates were then tested for antibiotic susceptibility test by modified Kirby-Bauer disk diffusion method and were subjected to ESBL screening by using 30 µg cefotaxime and ceftazidime. The ESBL production was confirmed by combination disk method. Results: From a total of 103 nonduplicated clinical isolates, E. coli (n = 79), Klebsiella pneumoniae (n = 18), and Klebsiella oxytoca (n = 6) were isolated from different clinical specimens. Of which, 64 (62.1%) exhibited multidrug resistance, and 29 (28.2%) were ESBL producers. All ESBL-producing isolates were resistant toward ampicillin, cefotaxime, ceftriaxone, and ceftazidime. Most ESBL producers were susceptible toward imipenem (89.7%; 26/29), nitrofurantoin (82.8%; 24/29), piperacillin/tazobactam (79.3%; 23/29), and amikacin (72.4%; 21/29). Conclusions: A high prevalence of multidrug-resistant ESBL organisms was found in this study among pediatric patients. Treatment based on their routine identification and susceptibility to specific antibiotics is critical to halt the spread of AMR and ESBL.

scholarly journals Emergence of an NDM-5-Producing Escherichia coli Sequence Type 410 Clone in Infants in a Children’s Hospital in China

2020 ◽  
Vol Volume 13 ◽  
pp. 703-710 ◽  
Author(s):  
Jun Li ◽  
Ting Yu ◽  
Xiao-Yan Tao ◽  
Yong-Mei Hu ◽  
Hai-Chen Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Type ◽  
Children's Hospital ◽  
Children’S Hospital

Trend of plasmid-mediated quinolone resistance genes at the Children’s Hospital in Tunisia

2014 ◽  
Vol 63 (2) ◽  
pp. 195-202 ◽  
Author(s):  
Nour El-Houda Jlili ◽  
Samia Réjiba ◽  
Hanen Smaoui ◽  
Thomas Guillard ◽  
Françoise Chau ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Random Amplified Polymorphic Dna ◽  
Enterobacter Cloacae ◽  
Quinolone Resistance ◽  
Children's Hospital ◽  
Extended Spectrum ◽  
High Prevalence ◽  
Children’S Hospital ◽  
Dna Pcr

The prevalence of plasmid-mediated quinolone resistance genes [qnr, aac(6′)-Ib-cr and qepA] was sought among Enterobacteriaceae strains obtained from the Children’s Hospital of Tunis (Tunisia). Non-duplicate isolates (n = 278) with resistance to extended-spectrum cephalosporins and collected in 2003, 2007, 2008 and 2009 were screened for qnr genes. Forty (14.4 %) isolates were qnr positive and were screened for the presence of the aac(6′)-Ib-cr and qepA genes. qnrB was detected in 21 Klebsiella pneumoniae, 11 Escherichia coli and 6 Enterobacter cloacae isolates. Sequence analysis of the qnrB amplicons revealed variants including 24 qnrB1, 11 qnrB2 and 3 qnrB6. qnrS (qnrS1 allele) was detected only in K. pneumoniae isolates, either alone (two isolates) or with the qnrB gene (one isolate). The qnrA, qnrC and qnrD genes were not found in any of the 278 isolates. No qnr-positive isolates carried the qepA gene. Pyrosequencing results showed that aac(6′)-Ib-cr, a variant of the aac(6′)-Ib gene, was present in 31 qnr-positive isolates (21 K. pneumoniae isolates, seven Escherichia coli isolates and three Enterobacter cloacae isolates). aac(6′)-Ib was also found either alone (two isolates) or in association with aac(6′)-Ib-cr (one isolate). Of the 40 qnr-positive isolates, 92.5, 82.5, 57.5, 85 and 82.5 % were non-susceptible to nalidixic acid, ciprofloxacin, levofloxacin, ofloxacin and norfloxacin, respectively, and all were extended-spectrum β-lactamase producers. Random amplified polymorphic DNA-PCR typing of these isolates showed 16, 8 and 5 different genotypes in K. pneumoniae, Escherichia coli and Enterobacter cloacae isolates, respectively. Our study highlights the high prevalence of qnr in association with aac(6′)-Ib-cr among Enterobacteriaceae isolates, even from children, who are patients not overtreated with quinolones.

scholarly journals KPC-2-producing Klebsiella pneumoniae ST11 in a Children’s Hospital in Poland

2017 ◽  
Vol 66 (3) ◽  
pp. 401-404 ◽  
Author(s):  
Monika Machulska ◽  
Anna Baraniak ◽  
Iwona Żak ◽  
Katarzyna Bojarska ◽  
Dorota Żabicka ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Klebsiella Pneumoniae ◽  
Teaching Hospital ◽  
Sequence Type ◽  
Children's Hospital ◽  
Extended Spectrum ◽  
Children’S Hospital ◽  
Resistance Patterns ◽  
Pediatric Center

Four Klebsiella pneumoniae isolates from children hospitalized over 10 months in an intensive care unit in a children’s teaching hospital in Poland were analyzed. All of the isolates belonged to a single pulsotype and sequence type (ST) 11, and produced the KPC-2 carbapenemase and extended-spectrum β-lactamase (ESBL) CTX-M-15. They were resistant to a variety of antimicrobials, and their β-lactam resistance patterns were typical for KPC producers. This is one of few cases of identification of KPC (or carbapenemase)-producing K. pneumoniae in a pediatric center in Poland.

scholarly journals Occurrence of blaKPC-2, blaCTX-M, and mcr-1 in Enterobacteriaceae from Well Water in Rural China

2017 ◽  
Vol 61 (4) ◽  
Author(s):  
Pan Sun ◽  
Zhenwang Bi ◽  
Maud Nilsson ◽  
Beiwen Zheng ◽  
Björn Berglund ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Rural China ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Well Water ◽  
Content Type ◽  
Potential Source ◽  
Extended Spectrum

ABSTRACT We report on the coexistence of mcr-1 and bla CTX-M in multidrug-resistant, extended-spectrum β-lactamase-producing Escherichia coli belonging to the sequence type 10 complex isolated from well water in rural China. Raoultella ornithinolytica with bla KPC-2 was also detected in well water from the same area. This study shows that genes coding for resistance to last-resort antibiotics are present in wells in rural China, indicating a potential source of antibiotic resistance.

scholarly journals Escherichia coli Sequence Type ST131 as an Emerging Fluoroquinolone-Resistant Uropathogen among Renal Transplant Recipients

2009 ◽  
Vol 54 (1) ◽  
pp. 546-550 ◽  
Author(s):  
James R. Johnson ◽  
Brian Johnston ◽  
Connie Clabots ◽  
Michael A. Kuskowski ◽  
Swaroop Pendyala ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Renal Transplant ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Black Race ◽  
E Coli ◽  
Extended Spectrum

ABSTRACT Among 40 Escherichia coli urine isolates from renal transplant recipients (Galveston, TX, 2003 to 2005), sequence type ST131 (O25:H4) was highly prevalent (representing 35% of isolates overall and 60% of fluoroquinolone-resistant isolates), virulent appearing, antimicrobial resistant (but extended-spectrum-cephalosporin susceptible), and associated with black race. Pulsotypes were diverse; some were linked to other locales. ST131 emerged significantly during the study period. These findings suggest that E. coli ST131 may constitute an important new multidrug-resistant threat to renal transplant recipients.

scholarly journals Travelers Can Import Colistin-Resistant Enterobacteriaceae, Including Those Possessing the Plasmid-Mediatedmcr-1Gene

2016 ◽  
Vol 60 (8) ◽  
pp. 5080-5084 ◽  
Author(s):  
Odette J. Bernasconi ◽  
Esther Kuenzli ◽  
João Pires ◽  
Regula Tinguely ◽  
Alessandra Carattoli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Type ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Extended Spectrum ◽  
Stool Samples ◽  
Plasmid Backbone

ABSTRACTStool samples from 38 travelers returning from India were screened for extended-spectrum cephalosporin- and carbapenem-resistantEnterobacteriaceaeimplementing standard selective plates. Twenty-six (76.3%) people were colonized with CTX-M or DHA producers, but none of the strains was colistin resistant and/ormcr-1positive. Nevertheless, using overnight enrichment and CHROMagar Orientation plates supplemented with colistin, four people (10.5%) were found to be colonized with colistin-resistantEscherichia coli. One cephalosporin-susceptible sequence type 10 (ST10) strain carried a 4,211-bp ISApl1-mcr-1-ISApl1element in an IncHI2 plasmid backbone.

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