scholarly journals Occurrence of blaKPC-2, blaCTX-M, and mcr-1 in Enterobacteriaceae from Well Water in Rural China

2017 ◽  
Vol 61 (4) ◽  
Author(s):  
Pan Sun ◽  
Zhenwang Bi ◽  
Maud Nilsson ◽  
Beiwen Zheng ◽  
Björn Berglund ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Rural China ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Well Water ◽  
Content Type ◽  
Potential Source ◽  
Extended Spectrum

ABSTRACT We report on the coexistence of mcr-1 and bla CTX-M in multidrug-resistant, extended-spectrum β-lactamase-producing Escherichia coli belonging to the sequence type 10 complex isolated from well water in rural China. Raoultella ornithinolytica with bla KPC-2 was also detected in well water from the same area. This study shows that genes coding for resistance to last-resort antibiotics are present in wells in rural China, indicating a potential source of antibiotic resistance.

scholarly journals Characteristics of Escherichia coli Sequence Type 131 Isolates That Produce Extended-Spectrum β-Lactamases: Global Distribution of theH30-Rx Sublineage

2014 ◽  
Vol 58 (7) ◽  
pp. 3762-3767 ◽  
Author(s):  
Gisele Peirano ◽  
Akke K. van der Bij ◽  
Joshua L. Freeman ◽  
Laurent Poirel ◽  
Patrice Nordmann ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
United Arab Emirates ◽  
The United States ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Global Distribution ◽  
Phylogenetic Groups ◽  
Content Type ◽  
Specific Pcr ◽  
Extended Spectrum

ABSTRACTWe designed a study to describe the characteristics of sequence type 131 (ST131) lineages, including theH30-Rx sublineage, among a global collection of extended-spectrum β-lactamase (ESBL)-producingEscherichia coliisolates from 9 countries collected from 2000 to 2011. A total of 240 nonrepeat isolates from Canada, the United States, Brazil, the Netherlands, France, the United Arab Emirates (UAE), India, South Africa, and New Zealand were included. Established PCR, sequencing, and typing methods were used to define ST131 lineages,H30 andH30-Rx phylogenetic groups,gyrAandparCmutations, virotypes, and plasmid-mediated quinolone resistance determinants. The majority of the isolates produced CTX-M-15 withaac(6′)-lb-cr, belonged to phylogenetic group B2, and were positive for theH30 lineage with thegyrA1ABandparC1aABmutations. ST131 showed 15 distinct pulsotypes; 43% of the isolates belonged to four pulsotypes, with a global distribution. Seventy-five percent of the ST131 isolates belonged toH30-Rx; this sublineage was present in all the countries and was associated with multidrug resistance,blaCTX-M-15,aac(6′)-lb-cr, and virotypes A and C. TheH41 lineage was negative for the ST131pabBallele-specific PCR. The multidrug-resistantH30-Rx sublineage poses an important public health threat due to its global distribution, association with virotype C, and high prevalence among ST131 isolates that produce CTX-M-15.

scholarly journals Escherichia coli Sequence Type ST131 as an Emerging Fluoroquinolone-Resistant Uropathogen among Renal Transplant Recipients

2009 ◽  
Vol 54 (1) ◽  
pp. 546-550 ◽  
Author(s):  
James R. Johnson ◽  
Brian Johnston ◽  
Connie Clabots ◽  
Michael A. Kuskowski ◽  
Swaroop Pendyala ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Renal Transplant ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Black Race ◽  
E Coli ◽  
Extended Spectrum

ABSTRACT Among 40 Escherichia coli urine isolates from renal transplant recipients (Galveston, TX, 2003 to 2005), sequence type ST131 (O25:H4) was highly prevalent (representing 35% of isolates overall and 60% of fluoroquinolone-resistant isolates), virulent appearing, antimicrobial resistant (but extended-spectrum-cephalosporin susceptible), and associated with black race. Pulsotypes were diverse; some were linked to other locales. ST131 emerged significantly during the study period. These findings suggest that E. coli ST131 may constitute an important new multidrug-resistant threat to renal transplant recipients.

scholarly journals Travelers Can Import Colistin-Resistant Enterobacteriaceae, Including Those Possessing the Plasmid-Mediatedmcr-1Gene

2016 ◽  
Vol 60 (8) ◽  
pp. 5080-5084 ◽  
Author(s):  
Odette J. Bernasconi ◽  
Esther Kuenzli ◽  
João Pires ◽  
Regula Tinguely ◽  
Alessandra Carattoli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Type ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Extended Spectrum ◽  
Stool Samples ◽  
Plasmid Backbone

ABSTRACTStool samples from 38 travelers returning from India were screened for extended-spectrum cephalosporin- and carbapenem-resistantEnterobacteriaceaeimplementing standard selective plates. Twenty-six (76.3%) people were colonized with CTX-M or DHA producers, but none of the strains was colistin resistant and/ormcr-1positive. Nevertheless, using overnight enrichment and CHROMagar Orientation plates supplemented with colistin, four people (10.5%) were found to be colonized with colistin-resistantEscherichia coli. One cephalosporin-susceptible sequence type 10 (ST10) strain carried a 4,211-bp ISApl1-mcr-1-ISApl1element in an IncHI2 plasmid backbone.

Antibiotic Resistance Profile of Commensal Escherichia coli Isolated from Broiler Chickens in Qatar

2017 ◽  
Vol 81 (2) ◽  
pp. 302-307 ◽  
Author(s):  
Nahla O. Eltai ◽  
Elmoubasher A. Abdfarag ◽  
Hamad Al-Romaihi ◽  
Eman Wehedy ◽  
Mahmoud H. Mahmoud ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Broiler Chickens ◽  
Susceptibility Testing ◽  
Multidrug Resistant ◽  
Test Method ◽  
Health Concern ◽  
E Coli ◽  
Extended Spectrum ◽  
Stewardship Program

ABSTRACT Antibiotic resistance (AR) is a growing public health concern worldwide, and it is a top health challenge in the 21st century. AR among Enterobacteriaceae is rapidly increasing, especially in third-generation cephalosporins and carbapenems. Further, strains carrying mobilized colistin resistance (mcr) genes 1 and 2 have been isolated from humans, food-producing animals, and the environment. The uncontrolled use of antibiotics in food-producing animals is a major factor in the generation and spread of AR. No studies have been done to evaluate AR in the veterinary sector of Qatar. This study aimed at establishing primary baseline data for the prevalence of AR among food-producing animals in Qatar. Fecal samples (172) were obtained from two broiler farms and one live bird market in Qatar, and 90 commensal Escherichia coli bacteria were isolated and subjected to susceptibility testing against 16 clinically relevant antibiotics by using the E-test method. The results found that 81 (90%) of 90 isolates were resistant to at least one antibiotic, 14 (15.5%) of 90 isolates were colistin resistant, 2 (2.2%) of 90 isolates were extended-spectrum β-lactamase producers, and 2 (2.2%) of 90 isolates were multidrug resistant to four antibiotic classes. Extended-spectrum β-lactamase–producing E. coli and colistin-resistant isolates were confirmed by using double-disc susceptibility testing and PCR, respectively. Such a high prevalence of antibiotic-resistant E. coli could be the result of a long application of antibiotic treatment, and it is an indicator of the antibiotic load in food-producing animals in Qatar. Pathogens carrying AR can be easily transmitted to humans through consumption of undercooked food or noncompliance with hygiene practices, mandating prompt development and implementation of a stewardship program to control and monitor the use of antibiotics in the community and agriculture.

scholarly journals A Comprehensive Account of Escherichia coli Sequence Type 131 in Wastewater Reveals an Abundance of Fluoroquinolone-Resistant Clade A Strains

2019 ◽  
Vol 86 (4) ◽  
Author(s):  
Thomas J. Finn ◽  
Lena Scriver ◽  
Linh Lam ◽  
Mai Duong ◽  
Gisele Peirano ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
International Health ◽  
Multidrug Resistant ◽  
Screening Strategy ◽  
Sequence Type ◽  
High Rate ◽  
Dominant Form ◽  
Content Type ◽  
Comprehensive Account ◽  
Tract Infections

ABSTRACT In the ten years since its discovery, the Escherichia coli clone sequence type 131 (ST131) has become a major international health threat, with the multidrug-resistant and extended-spectrum β-lactamase (ESBL)-producing clade C emerging as the globally dominant form. ST131 has previously been isolated from wastewater; however, most of these studies selectively screened for ESBL-producing organisms, thereby missing the majority of remaining ST131 clades. In this study, we used a high-throughput PCR-based screening strategy to comprehensively examine wastewater for the presence of ST131 over a 1-year period. Additional multiplex PCRs were used to differentiate clades and obtain an unbiased account of the total ST131 population structure within the collection. Furthermore, antimicrobial susceptibility profiles of all ST131-positive samples were tested against a range of commonly used antibiotics. From a total of over 3,762 E. coli wastewater samples, 1.86% (n = 70) tested positive for ST131, with the majority being clade A isolates. In total, 63% (n = 44) were clade A, 29% (n = 20) were clade B, 1% (n = 1) were clade C0, 6% (n = 4) were clade C1, and 1% (n = 1) were clade C2. In addition, a very high rate of resistance to commonly used antibiotics among wastewater isolates is reported, with 72.7% (n = 32) of clade A resistant to ciprofloxacin and high rates of resistance to gentamicin, sulfamethoxazole-trimethoprim, and tetracycline in clades that are typically sensitive to antibiotics. IMPORTANCE ST131 is a global pathogen. This clone causes urinary tract infections and is frequently isolated from human sources. However, little is known about ST131 from environmental sources. With the widely reported increase in antibiotic concentrations found in wastewater, there is additional selection pressure for the emergence of antibiotic-resistant ST131 in this niche. The unbiased screening approach reported herein revealed that previously antibiotic-sensitive lineages of ST131 are now resistant to commonly used antibiotics present in wastewater systems and may be capable of surviving UV sterilization. This is the most comprehensive account of ST131 in the wastewater niche to date and an important step in better understanding the ecology of this global pathogen.

scholarly journals Detection of an Escherichia coli Sequence Type 167 Strain with Two Tandem Copies ofblaNDM-1in the Chromosome

2016 ◽  
Vol 55 (1) ◽  
pp. 199-205 ◽  
Author(s):  
Ping Shen ◽  
Maoli Yi ◽  
Ying Fu ◽  
Zhi Ruan ◽  
Xiaoxing Du ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Insertion Sequence ◽  
Southern Blotting ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
New Delhi ◽  
Genomic Context ◽  
Content Type ◽  
Link Type ◽  
Reference Genomes

ABSTRACTNew Delhi metallo-β-lactamase-1 (NDM-1)-producingEnterobacteriaceaehas disseminated rapidly throughout the world and poses an urgent threat to public health. Previous studies confirmed that theblaNDM-1gene is typically carried in plasmids but rarely in chromosome. We discovered a multidrug-resistantEscherichia colistrain Y5, originating from a urine sample and containing theblaNDM-1gene, which did not transfer by either conjugation or electrotransformation. We confirmed the possibility of its chromosome location by S1-pulsed-field gel electrophoresis (PFGE) and XbaI-PFGE, followed by Southern blotting. To determine the genomic background ofblaNDM-1, the genome of Y5 was completely sequenced and compared to other reference genomes. The results of our study revealed that this isolate consists of a 4.8-Mbp chromosome and three plasmids, it is an epidemic clone of sequence type (ST) 167, and it shows 99% identity withEscherichia coli6409 (GenBank accession no.CP010371), which lacks the sameblaNDM-1gene-surrounding structure as Y5. TheblaNDM-1gene is embedded in the chromosome along with two tandem copies of an insertion sequence common region 1 (ISCR1) element (sul1-ARR-3-cat-blaNDM-1-bleo-ISCR1), which appears intact in the plasmid fromProteus mirabilis(GenBank accession no.KP662515). The genomic context indicates that the ISCR1element mediated theblaNDM-1transposition from a single source plasmid to the chromosome. Our study is the first report of anEnterobacteriaceaestrain harboring a chromosomally integratedblaNDM-1, which directly reveals the vertical spreading pattern of the gene. Close surveillance is urgently needed to monitor the emergence and potential spread of ST167 strains that harborblaNDM-1.

scholarly journals Complete Sequencing of Plasmids ContainingblaOXA-163andblaOXA-48in Escherichia coli Sequence Type 131

2016 ◽  
Vol 60 (11) ◽  
pp. 6948-6951 ◽  
Author(s):  
Nicole Stoesser ◽  
Anna E. Sheppard ◽  
Gisele Peirano ◽  
Robert Sebra ◽  
Tarah Lynch ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Sequence Type ◽  
Content Type ◽  
Extended Spectrum ◽  
Complete Sequencing

ABSTRACTOXA-48-like enzymes have emerged as important extended-spectrum β-lactamases/carbapenemases inEscherichia colisequence type 131 (ST131). We report the structures of the first fully sequencedblaOXA-163plasmid and of two otherblaOXA-48plasmids in this lineage.blaOXA-163was located on a 71-kb IncN plasmid with other resistance genes.blaOXA-48was present on IncL/M plasmids, genetically similar to otherblaOXA-48plasmid sequences, and consistent with interspecies/interlineage spread. The presence ofblaOXA-48-likegenes on epidemic plasmids in ST131 is of concern.

scholarly journals Activity of Eravacycline against Escherichia coli Clinical Isolates Collected from U.S. Veterans in 2011 in Relation to Coresistance Phenotype and Sequence Type 131 Genotype

2015 ◽  
Vol 60 (3) ◽  
pp. 1888-1891 ◽  
Author(s):  
James R. Johnson ◽  
Stephen B. Porter ◽  
Brian D. Johnston ◽  
Paul Thuras
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Veterans Affairs ◽  
Sequence Type ◽  
Clinical Isolates ◽  
Gram Negative ◽  
Content Type ◽  
Medical Centers ◽  
Resistant Strains ◽  
Veterans Affairs Medical Centers

Eravacycline is a novel broad-spectrum fluorocycline with potent Gram-negative activity, including for multidrug-resistant strains. Among 472Escherichia coliclinical isolates from 24 Veterans Affairs medical centers (in 2011), divided equally as susceptible versus resistant to fluoroquinolones, broth microdilution eravacycline MICs were distributed unimodally, ranging from 0.03 to 1.0 μg/ml (MIC50of 0.125 μg/ml, MIC90of 0.25 μg/ml). Eravacycline MICs were ∼2-fold higher among fluoroquinolone-resistant, gentamicin-resistant, multidrug-resistant, and sequence type 131 (ST131) isolates (P< 0.01 for each comparison).

scholarly journals Draft Genome Sequences of Two Multidrug-Resistant Sequence Type 405 Escherichia coli Isolates without Clinical Infection

2021 ◽  
Vol 10 (31) ◽  
Author(s):  
Amanda Chamieh ◽  
Rita Zgheib ◽  
Sabah El-Sawalhi ◽  
Eid Azar ◽  
Jean-Marc Rolain
Keyword(s):  
Escherichia Coli ◽  
Draft Genome ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Clinical Isolates ◽  
Clinical Infection ◽  
Genome Sequences ◽  
Content Type

We present the genome sequences of two carbapenemase-producing sequence type 405 Escherichia coli clinical isolates, strains Marseille-Q1950 and Marseille-Q1951. The isolates were obtained 1 month apart during the patient’s hospitalization in Lebanon, in May (Marseille-Q1950) and June (Marseille-Q1951) 2019. The genome sizes of strains Marseille-Q1950 and Marseille-Q1951 were 5,181,515 bp and 5,213,451 bp, respectively.

scholarly journals Transmission of NDM-5-Producing and OXA-48-Producing Escherichia coli Sequence Type 648 by International Visitors without Previous Medical Exposure

2021 ◽  
Vol 9 (3) ◽  
Author(s):  
Sohei Harada ◽  
Masahiro Suzuki ◽  
Toshiharu Sasaki ◽  
Aki Sakurai ◽  
Masato Inaba ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Risk ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Resistant Clone ◽  
Content Type ◽  
Multidrug Resistant Organisms ◽  
History Of ◽  
Medical Exposure ◽  
International Visitors

Although patients with history of international hospitalization are often subject to screening for multidrug-resistant organisms, it is unclear whether patients who reside in countries where carbapenemase-producing Enterobacterales (CPE) is endemic but have no history of local hospitalization contribute to the transmission of CPE. In this study, NDM-5-producing and OXA-48-producing Escherichia coli sequence type (ST) 648, a recently recognized high-risk, multidrug-resistant clone, were detected from two overseas visitors without previous medical exposure.

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