Structural characterization of a pectic polysaccharide from Codonopsis pilosula and its immunomodulatory activities in vivo and in vitro

Development of genetic competence in Bacillus subtilis results in expression of late competence genes, including comFB. In vitro and in vivo studies revealed that ComFB dimerizes, binds zinc, and possibly plays a regulatory role in competence development.

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Structural Characterization of in Vitro and in Vivo Intermediates on the Loading Module of Microcystin Synthetase

ACS Chemical Biology ◽

10.1021/cb500007v ◽

2006 ◽

Vol 1 (2) ◽

pp. 93-102 ◽

Cited By ~ 37

Author(s):

Leslie M. Hicks ◽

Michelle C. Moffitt ◽

Laura L. Beer ◽

Bradley S. Moore ◽

Neil L. Kelleher

Keyword(s):

Structural Characterization ◽

Microcystin Synthetase

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Structural characterization of novel comb-like branched α-d-glucan from Arca inflata and its immunoregulatory activities in vitro and in vivo

Food & Function ◽

10.1039/c9fo01395d ◽

2019 ◽

Vol 10 (10) ◽

pp. 6589-6603

Author(s):

Chunlei Li ◽

Dan Peng ◽

Weijuan Huang ◽

Xiaozheng Ou ◽

Liyan Song ◽

...

Keyword(s):

Structural Characterization ◽

Water Soluble ◽

Soluble Polysaccharide

In the current study, we identified and characterized a novel water-soluble polysaccharide (JNY2PW) with significant immunoregulatory effects and no apparent overall toxicity.

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Structural characterization of a new steroidal saponin from Agave angustifolia var. Marginata and a preliminary investigation of its in vivo antiulcerogenic activity and in vitro membrane permeability property

Bioorganic & Medicinal Chemistry Letters ◽

10.1016/j.bmcl.2017.08.026 ◽

2017 ◽

Vol 27 (18) ◽

pp. 4345-4349 ◽

Cited By ~ 2

Author(s):

Gabriela Moysés Pereira ◽

Marcela Gonçalves Ribeiro ◽

Bernadete Pereira da Silva ◽

José Paz Parente

Keyword(s):

Membrane Permeability ◽

Structural Characterization ◽

Preliminary Investigation ◽

Steroidal Saponin ◽

Permeability Property ◽

Agave Angustifolia ◽

Antiulcerogenic Activity

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Bioglass 45S5: Structural characterization of short range order and analysis of biocompatibility with adipose-derived mesenchymal stromal cells in vitro and in vivo

Materials Science and Engineering C ◽

10.1016/j.msec.2019.109781 ◽

2019 ◽

Vol 103 ◽

pp. 109781 ◽

Cited By ~ 6

Author(s):

Cristiano Rodrigues ◽

Liliana Ivet Sous Naasani ◽

Carla Zanatelli ◽

Thaís Casagrande Paim ◽

Jéssica Gonçalves Azevedo ◽

...

Keyword(s):

Mesenchymal Stromal Cells ◽

Structural Characterization ◽

Stromal Cells ◽

Short Range ◽

Short Range Order ◽

Range Order

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In vivo and in vitro characterization of immediate release dosage forms containing n-acetylcysteine using the dynamic open flow-through test apparatus

10.26226/morressier.57d6b2b7d462b8028d88dd29 ◽

2016 ◽

Author(s):

Maximilian Sager

Keyword(s):

Immediate Release ◽

Dosage Forms ◽

Test Apparatus ◽

Open Flow ◽

In Vitro Characterization ◽

Flow Through

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Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid-beta M1-42

10.26434/chemrxiv.7725002.v1 ◽

2019 ◽

Author(s):

Priya Prakash ◽

Travis Lantz ◽

Krupal P. Jethava ◽

Gaurav Chopra

Keyword(s):

Amyloid Beta ◽

Western Blots ◽

Force Microscopy ◽

E Coli ◽

Atomic Force ◽

Set Up ◽

Short Time

Amyloid plaques found in the brains of Alzheimer’s disease (AD) patients primarily consists of amyloid beta 1-42 (Ab42). Commercially, Ab42 is synthetized using peptide synthesizers. We describe a robust methodology for expression of recombinant human Ab(M1-42) in Rosetta(DE3)pLysS and BL21(DE3)pLysS competent E. coli with refined and rapid analytical purification techniques. The peptide is isolated and purified from the transformed cells using an optimized set-up for reverse-phase HPLC protocol, using commonly available C18 columns, yielding high amounts of peptide (~15-20 mg per 1 L culture) in a short time. The recombinant Ab(M1-42) forms characteristic aggregates similar to synthetic Ab42 aggregates as verified by western blots and atomic force microscopy to warrant future biological use. Our rapid, refined, and robust technique to purify human Ab(M1-42) can be used to synthesize chemical probes for several downstream in vitro and in vivo assays to facilitate AD research.

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