Sequential aqueous acetone fractionation and characterization of Brauns native lignin separated from Chinese quince fruit

2022 ◽  
Author(s):  
Xi-Chuang Cheng ◽  
Xin-Ran Guo ◽  
Zhao Qin ◽  
Hua-Min Liu ◽  
Jing-Ren He ◽  
...  
Keyword(s):  
Aqueous Acetone ◽  
Chinese Quince ◽  
Acetone Fractionation

scholarly journals Aqueous acetone fractionation of kraft, organosolv and soda lignins

2018 ◽  
Vol 106 ◽  
pp. 979-987 ◽  
Author(s):  
Juan Domínguez-Robles ◽  
Tarja Tamminen ◽  
Tiina Liitiä ◽  
María Soledad Peresin ◽  
Alejandro Rodríguez ◽  
...  
Keyword(s):  
Aqueous Acetone ◽  
Acetone Fractionation

Enolase from Lobster (Homarus americanus)

1971 ◽  
Vol 28 (6) ◽  
pp. 879-882 ◽  
Author(s):  
M. John Chapman ◽  
Christopher Chin ◽  
Finn Wold
Keyword(s):  
Heat Treatment ◽  
Ion Exchange ◽  
Ammonium Sulfate ◽  
Catalytic Properties ◽  
Gel Filtration ◽  
Homarus Americanus ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Acetone Fractionation

Enolase has been isolated from lobster muscle by acetone fractionation, heat treatment, ammonium sulfate fractionation, gel filtration, and ion-exchange chromatography. Preliminary characterization of the pure enzyme shows that the catalytic properties are very similar to those of the enolases from rabbit and fish.

scholarly journals Heterologous Expression and Characterization of Thermostable Lipases from Geobacillus thermoleovorans PPD2 through Escherichia coli

2017 ◽  
Vol 14 (3) ◽  
pp. 1081-1088 ◽  
Author(s):  
Anita H. Permana ◽  
Fida Madayanti Warganegara ◽  
Deana Wahyuningrum ◽  
Made Puspasari Widhiastuty ◽  
Akhmaloka Akhmaloka
Keyword(s):  
Escherichia Coli ◽  
Heterologous Expression ◽  
Lipolytic Activity ◽  
Escherichia Coli Bl21 ◽  
Geobacillus Thermoleovorans ◽  
Medium Chain Length ◽  
Dominant Band ◽  
Acetone Fractionation ◽  
Thermostable Lipases

ABSTRACT: Heterologous expression and purification of thermostable lipase from Geobacillus thermoleovorans PPD2 had been carried out through Escherichia coli BL21 as host. Two bands obtained showed lipolytic activity with the size at around 51 (LipA) and 43 (LipB) kDa, respectively. The activities were identified by zymogram analysis, while the control protein from Escherichia coli BL21(DE3) do not show any lipolytic activity. Purification of crude extract using chromatography affinity Ni-NTA resulted one dominant band of LipA, meanwhile LipB did not appeared on the gel. Another purification for LipB was carried out by acetone fractionation. Both of LipA and LipB showed high activity toward medium chain length substrates, with optimum activity at 50oC and pH 8.5. The activities of LipA and LipB showed tolerance toward short chain alcohols, such as methanol, ethanol, n-propanol, and isopropanol.

Structural characterization of Chinese quince fruit lignin pretreated with enzymatic hydrolysis

2018 ◽  
Vol 262 ◽  
pp. 212-220 ◽  
Author(s):  
Zhao Qin ◽  
Xue-De Wang ◽  
Hua-Min Liu ◽  
Dong-Min Wang ◽  
Guang-Yong Qin
Keyword(s):  
Enzymatic Hydrolysis ◽  
Structural Characterization ◽  
Chinese Quince

Purification and Characterization of a Lectin from Chinese Quince (Chaenomeles sinensis) Fruit

2010 ◽  
Vol 19 (2) ◽  
pp. 243-245 ◽  
Author(s):  
Atsuya Ishiwari ◽  
Tomoko Akutsu ◽  
Hiroshi Ikegaya ◽  
Koichi Sakurada
Keyword(s):  
Purification And Characterization ◽  
Chaenomeles Sinensis ◽  
Chinese Quince

scholarly journals Isolation and Characterization of Heparin From Tuna Skins

2007 ◽  
Vol 13 (2) ◽  
pp. 137-145 ◽  
Author(s):  
Walter P. Jeske ◽  
Meredith K. McDonald ◽  
Debra A. Hoppensteadt ◽  
Elaine C. Bau ◽  
Aline Mendes ◽  
...  
Keyword(s):  
Partial Thromboplastin Time ◽  
Anticoagulant Activity ◽  
Economic Value ◽  
Activated Partial Thromboplastin Time ◽  
Biologically Active ◽  
Raw Material ◽  
Isolation And Characterization ◽  
Acetone Fractionation ◽  
Antiprotease Activity

This study characterized heparin isolated from tuna skins. Glycosaminoglycans were isolated from tuna skin after digestion using anion exchange resin. Heparin was eluted from the resin by sodium chloride gradient and was further fractionated by acetone fractionation. Anticoagulant activity was determined using the activated partial thromboplastin time and Heptest assays. Potency was determined using amidolytic antifactor IIa and antifactor Xa assays. The presence of heparin in the extracted tuna skin glycosaminoglycans was confirmed using 13C-nuclear magnetic resonance. The activated partial thromboplastin time and Heptest clotting times were doubled at concentrations of about 4 and 1 µg/mL, respectively. The clotting time prolongation and antiprotease activity induced by tuna heparin was readily neutralized by 25 µg/mL protamine sulfate. These results demonstrate that biologically active heparin with properties similar to clinical grade heparin can be derived from tuna skin, a raw material with otherwise relatively little economic value.

Characterization of aroma-active compounds in Chinese quince ( Pseudocydonia sinensis Schneid) by aroma dilution analyses

2018 ◽  
Vol 105 ◽  
pp. 828-835 ◽  
Author(s):  
Ji Young Choi ◽  
Sang Mi Lee ◽  
Hyong Joo Lee ◽  
Young-Suk Kim
Keyword(s):  
Active Compounds ◽  
Chinese Quince

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

AEM analysis of crystallization in Ti-based amorphous alloys

1983 ◽  
Vol 41 ◽  
pp. 270-271
Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee
Keyword(s):  
Magnetic Properties ◽  
Amorphous Alloys ◽  
Amorphous Materials ◽  
Isothermal Annealing ◽  
Amorphous Matrix ◽  
Nucleation And Growth ◽  
Current Interest ◽  
Amorphous Films ◽  
Electrical And Magnetic Properties

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.

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