Identification of Bacillus spp., Escherichia coli, Salmonella spp., Staphylococcus spp. and Vibrio spp. with 16S ribosomal DNA-based oligonucleotide array hybridization

2006 ◽  
Vol 107 (2) ◽  
pp. 131-137 ◽  
Author(s):  
Yu-Cheng Chiang ◽  
Chi-Yea Yang ◽  
Chin Li ◽  
Yi-Cheng Ho ◽  
Chien-Ku Lin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Dna ◽  
Oligonucleotide Array ◽  
Salmonella Spp ◽  
16S Ribosomal Dna ◽  
Bacillus Spp ◽  
Array Hybridization ◽  
Staphylococcus Spp ◽  
Vibrio Spp

scholarly journals Evaluation of sanitary quality of goat milk in dairy industries from the Cariri region, state of Paraíba

2021 ◽  
Vol 10 (12) ◽  
pp. e596101220735
Author(s):  
Iara Nunes de Siqueira ◽  
Aline Antas Cordeiro Cavalcanti ◽  
Joyce Galvão de Souza ◽  
Filipe Jordão Pereira de Medeiros ◽  
João Carlos Taveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Pathogenic Bacteria ◽  
Goat Milk ◽  
Salmonella Spp ◽  
Pasteurized Milk ◽  
Enterococcus Spp ◽  
Bacillus Spp ◽  
Thermotolerant Coliforms ◽  
Staphylococcus Spp

The sanitary evaluation of equipment and hands is fundamental to investigate the presence of pathogens in the dairy industry. Then, this study aims to evaluate the sanitization of equipment, workers’ hands, raw and pasteurized milk in goat milk dairies in the Cariri region, state of Paraíba.  Collected 32 samples of four dairies represented by letters A, B, C, and D. The followings contents were analyzed: mesophiles, total and thermotolerant coliforms, Escherichia coli, Staphylococcus aureus, Samonella spp. and Listeria monocytogenes in the reception tank, pasteurization tank, packing machine, package, wall, workers’ hand, and each dairy’s raw and pasteurized milk. After isolation, 84 colonies were confirmed by MALDI TOF. The indicator microorganisms presented variations for the workers’ hands, while A and B stayed within the patterns. For the equipment, only dairy B was within limits. They were out of the standard for mesophiles, total coliforms, and thermotolerant regarding raw and pasteurized milk. The microorganisms, the Enterobacteriaceae family presented a higher frequency, with 77.38%, and within this family, Escherichia coli, Klebsiella spp., and Enterobacter spp. were the most prevalent. Gram-positive corresponded to 22.62%, Bacillus spp., Staphylococcus spp., Enterococcus spp., and Macrococcus caseolyticus. Listeria monocytogenes and Salmonella spp. were not isolated. These demonstrate failures in goat milk processing with pathogenic bacteria in several dairy plants, indicating the need to adjust the product’s quality control.

scholarly journals Microbial assessment of different samples of ostrich (Struthio camelus) and determination of antimicrobial susceptibility profiles of the isolated bacteria

2018 ◽  
Vol 3 (4) ◽  
pp. 437-445
Author(s):  
Israt Jahan ◽  
Nazmi Ara Rumi ◽  
Md Khaled Hossain ◽  
Md Shajedur Rahman ◽  
Md Fakhruzzaman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cloacal Swab ◽  
Plate Count ◽  
Salmonella Spp ◽  
Plate Count Agar ◽  
Antimicrobial Sensitivity ◽  
Bacillus Spp ◽  
Oropharyngeal Swab ◽  
Susceptibility Profiles ◽  
Staphylococcus Spp

The study was conducted to monitor the microbial assessment in oropharyngeal swab, cloacal swab and faecal samples of ostrich and also update knowledge on antimicrobial sensitivity of isolated organisms. The study was performed at Hajee Mohammad Danesh Science & Technology University (HSTU) ostrich farm from January to June, 2016. A total 75 samples were randomly collected from 8 ostriches at different age of which 25 oropharyngeal, 25 cloacal swabs sample and 25 were faeces samples. The organisms were isolated by using standard microbiological method. The result revealed that the average microbial load in plate count agar was highest in faeces (1.48×109±0.27 CFU) than oropharyngeal swab (1.36×109±0.38 CFU) and cloacal swab (1.28×109±0.34 CFU). Among 75 samples, 29 (38.66%) Escherichia coli, 21 (28%) Salmonella spp., 20 (26.67%) Staphylococcus spp. and 5(6.6%) Bacillus spp. were identified. In 25 oropharyngeal swab samples, Staphylococcus spp. was found highest 15 (60%) than other organisms but Escherichia coli were found highest both in cloacal 11(44%) and faecal 12(48%) samples. On antibiogram study Escherichia coli, Salmonella spp., Staphylococcus spp. and Bacillus spp. were more sensitive to Levofloxacin, Azithromycin, Nalidixic Acid and Amoxicillin respectively. While more resistant to Azithromycin, Penicillin, Chloramphenicol and Erythromycin respectively. The obtained results indicated that ostrich excreta are one of the most important sources of Escherichia coli, Staphylococcus spp., Salmonella spp., Bacillus spp. in ostrich farms. Finally, it may be concluding that the logical use of antibiotics must be adopted in ostrich farms reared in Bangladesh for prevention of the appearance of multidrug resistance bacteria. Moreover, proper measures should be taken to aware off zoonotic diseases in peoples who are involved in ostrich farming.Asian J. Med. Biol. Res. December 2017, 3(4): 437-445

Microbiological Quality and Safety of Ready-to-Eat Street-Vended Foods in Johannesburg, South Africa

1999 ◽  
Vol 62 (11) ◽  
pp. 1278-1284 ◽  
Author(s):  
FRANCINA M. MOSUPYE ◽  
ALEXANDER von HOLY
Keyword(s):  
South Africa ◽  
Foodborne Pathogens ◽  
Water Samples ◽  
Microbiological Quality ◽  
Food Samples ◽  
Quality And Safety ◽  
Salmonella Spp ◽  
E Coli ◽  
Bacillus Spp ◽  
Staphylococcus Spp

Fifty-one ready-to-eat street foods, 18 dishwater, and 18 surface swab samples were collected from six vendors in Johannesburg, South Africa. Food temperatures were recorded at the time of sampling. Standard methods were used to determine aerobic plate counts (APCs), spore counts (SCs), and Enterobacteriaceae counts (ECs) for food samples as well as coliform counts (CCs) for water and swab samples. In addition, Petrifilm Escherichia coli count (PC) plates were used for the enumeration of coliforms in food, water, and swab samples. The presence of selected foodborne pathogens in the food samples as well as the presence of nonpathogenic E. coli 1 (in food and water samples) was also tested for. Predominant colonies isolated from APC plates were characterized to the genus level. Holding temperatures for cooked meats and gravies ranged from 42.0 to 94.0°C, and those for uncooked salads ranged from 29.0 to 39.0°C. Mean APC values of 3.4 (±0.4) log CFU/g, 4.0 (±1.2) log CFU/ml, and 2.1 (±0.4) log CFU/25 cm2 were obtained for food, water, and swab samples, respectively. Mean SC values of 1.6 (±0.2) log CFU/g and 1.5 (±0.3) log CFU/25 cm2 were obtained for food and swab samples, respectively. A mean EC value of 2.0 (±0.4) log CFU/g for food samples and mean CC values of 2.5 (±0.3) log CFU/ml and 1.3 (±0.3) log CFU/25 cm2 for water and swab samples, respectively, were determined. Mean PC values of 1.6 (±0.1) log CFU/g, 1.9 (±0.6) log CFU/ml, and 1.4 (±0.4) log CFU/25 cm2 were determined for food, water, and swab samples, respectively. Bacillus cereus was detected in 22%, Clostridium perfringens in 16%, Salmonella spp. in 2%, and E. coli (non-O157:H+) in 2% of the 51 food samples. E. coli was found in 14 water samples (78%) and in 3 food samples (6%). Campylobacter spp., Listeria monocytogenes, Staphylococcus aureus, Vibrio cholerae, and Yersinia enterocolitica were also tested for in the food samples, but they were not detected. The 340 isolates obtained from APC plates for food, water, and swab samples were predominantly Bacillus spp., Micrococcus spp., and Staphylococcus spp. for all three sample types. It was concluded that the foods analyzed in this study were of acceptable quality and safety.

scholarly journals Análisis microbiológico de dos muestras de pescado comercializadas en Tunja, Boyacá. Estudio de caso

2019 ◽  
pp. 52-65
Author(s):  
Gabriela Sanabria Sánchez ◽  
Jhojan Camilo Chiquillo Pompeyo
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Oncorhynchus Mykiss ◽  
Salmonella Spp ◽  
Vibrio Spp ◽  
Estudio De Caso

La producción pesquera en Colombia representa una actividad económica creciente. Entre las especies más consumidas, se encuentran la trucha arcoíris (Oncorhynchus mykiss) y el bagre (Brachyplatistoma sp.). El primero fue introducido al país a finales de la década de los 30, mientras que el bagre es una especie propia de Latinoamérica. El objetivo de la presente investigación fue determinar la calidad microbiológica de una trucha y un bagre comercializados en Tunja, y realizar una comparación microbiológica de las dos muestras. Para ello, se emplearon medios selectivos y diferenciales para el aislamiento de patógenos como coliformes, Salmonella spp., Vibrio spp. y Staphylococcus aureus coagulasa positiva. Los resultados evidenciaron la presencia de cepas de Salmonella spp., Escherichia coli, y Vibrio spp. Finalmente, se concluye que las muestras analizadas, pese a ser de diferente origen comercial y contar con condiciones de hábitat y alimentación completamente diferentes, no cumplen con los requisitos microbiológicos para ser comercializadas y podrían representar una potencial amenaza para la salud del consumidor.

scholarly journals Antibiogram of Bacteria Isolated from Tympanotonus fuscatus Var. Radula (Prosobranchia:Potamididae) Sold in Markets in Nasarawa State, Nigeria

2020 ◽  
pp. 1-9
Author(s):  
U. K. Asemota ◽  
M. D. Makut ◽  
S. O. Obiekezie ◽  
J. E. Owuna ◽  
M. O. Adamu
Keyword(s):  
Escherichia Coli ◽  
Bacterial Load ◽  
Bacterial Count ◽  
Total Bacterial Count ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Pseudomonas Spp ◽  
Gram Positive Bacteria ◽  
Bacillus Spp ◽  
Gram Negative Organisms

The aim of this study was to determine the antibiogram of bacterial isolates from Tympanotonus fuscatus var. radula sold in markets in Nasarawa State. Nigeria. Samples of Tympanotonus fuscatus var. radula (periwinkles) were bought from soup ingredient sellers at different sale locations in Keffi, Masaka and Orange markets and were analyzed using standard bacteriological methods. The bacterial isolates were identified using morphological, cultural and biochemical techniques. The total bacteria count varied from 1.18–3.20 x 108 CFU/g for the raw samples while the total bacterial count for the boiled samples varied from 0–1.57 x 108 CFU/g. Periwinkle samples with shells from Masaka market had the highest bacterial load with a mean total bacterial count of 2.94 x 10⁸ CFU/g and mean total coliform count of 2.80 x 10⁶ CFU/g. Raw periwinkle samples with shells had a higher bacterial load than samples without shells. There was also a drastic reduction in the bacterial load in the periwinkle samples after boiling under laboratory conditions. The bacteria isolated were Bacillus spp. and Staphylococcus aureus were the Gram-positive bacteria isolated. Enterobacter spp., Escherichia coli, Salmonella spp., Pseudomonas spp., Serratia spp. and Proteus spp. The most frequently occurring gram positive bacteria was Escherichia coli with an isolation frequency of 6(24%), the least frequently occurring was Bacillus spp., 8(32)%. Antibiotic susceptibility test showed that all the gram negative organisms exhibited sensitivity to ciprofloxacin: Escherichia coli (32 mm), Enterobacter spp. (41.5 mm), Proteus spp. (40.0 mm), Salmonella spp. (37.0 mm), Serratia spp. (26.0 mm), Pseudomonas spp. (23.0 mm). All the gram negative organisms showed marked resistance to vancomycin: Escherichia coli (12.0 mm), Enterobacter spp. (10.0 mm), Proteus spp. (11.0 mm), Salmonella spp. (5.0 mm), Serratia spp. (10.0 mm) and Pseudomonas spp. (4.5 mm).

scholarly journals Identification and antibiogram study of bacteria isolated from different street food

2018 ◽  
Vol 4 (3) ◽  
pp. 279-287
Author(s):  
Mohamed Ahmed Khalif ◽  
Md Khaled Hossain ◽  
Nazmi Ara Rumi ◽  
Md Shajedur Rahman ◽  
Md Aoulad Hosen
Keyword(s):  
Escherichia Coli ◽  
Bacterial Contamination ◽  
Wide Spectrum ◽  
Salmonella Spp ◽  
Street Vendor ◽  
Street Food ◽  
Food Borne ◽  
Shigella Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Food borne diseases are an increasingly recognized problem involving a wide spectrum of illnesses caused by bacterial contamination of food.Microorganism poses potential human health problems and is mainly transmitted through consumption of contaminated foods .Bangladesh is one of the densely populated country where majority of the people consume cheap foods prepared in unlicensed food selling points. The objective of the present study was to investigate the microbiological quality of different street food (Amra, Fusca, Chanachur and Guava) sold by various street vendor at Dinajpur, Bangladesh. A total of 20 samples were collected randomly from street vendors and tested for the presence of bacteria following standard microbiological method used for isolation, enumaration and identification of bacreria. Among the samples all had bacterial contamination. The total viable count (TVC) in diferrent street food samples was ranged from 8.0 × 105 CFU/g to 6.7x107 CFU/g. Among samples Escherichia coli 7(35%), Staphylococcus spp. 6(30%), Klebsiella spp. 5(25%), Salmonella spp. 1(5%) and Shigella spp. 1 (5%) were isolated. Escherichia coli were found highest 3(50%) Amra samples and 2(40%) Guava samples, then another organisms Staphylococcus spp. was found highest 2(50%) Chanachur and 2(40%) Fusca samples and then Klebsiella spp. was found highest 2(40%) Fusca samples. Antibiotic sensitivity test showed that Shigella spp., Staphylococcus spp., Klebsiella spp., Salmonella spp. and Escherichia coli were sensitive to Ciprofloxacin and Salmonella spp. were resistance to Gentamycin. All isolates found resistant to Cefixime, Cefalexin, Erythromycin, Fusidic acid, Cefuroxime and Aztreonam. All of the sample harbor multidrug resistant food borne bacteria which might cause public health hazards if these antibiotic resistance transfer to human.Asian J. Med. Biol. Res. September 2018, 4(3): 279-287

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