scholarly journals Increasing prevalence of multidrug-resistant mcr-1-positive Escherichia coli isolates from fresh vegetables and healthy food animals in South Korea

2020 ◽  
Vol 92 ◽  
pp. 53-55 ◽  
Author(s):  
Sung-Suck Oh ◽  
Jihyun Song ◽  
Junghee Kim ◽  
Jinwook Shin
Keyword(s):  
Escherichia Coli ◽  
South Korea ◽  
Multidrug Resistant ◽  
Healthy Food ◽  
Fresh Vegetables ◽  
Food Animals

scholarly journals IncA/C Plasmid-Mediated Spread of CMY-2 in Multidrug-Resistant Escherichia coli from Food Animals in China

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e96738 ◽  
Author(s):  
Yu-Fang Guo ◽  
Wen-Hui Zhang ◽  
Si-Qi Ren ◽  
Lin Yang ◽  
Dian-Hong Lü ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Food Animals

scholarly journals Acquisition of mcr-1 and Cocarriage of Virulence Genes in Avian Pathogenic Escherichia coli Isolates from Municipal Wastewater Influents in Japan

2019 ◽  
Vol 85 (22) ◽  
Author(s):  
Wataru Hayashi ◽  
Hayato Tanaka ◽  
Yui Taniguchi ◽  
Masaki Iimura ◽  
Eiji Soga ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Genes ◽  
Sequence Similarity ◽  
Multidrug Resistant ◽  
Common Source ◽  
High Sequence Similarity ◽  
Content Type ◽  
E Coli ◽  
Query Coverage ◽  
Food Animals

ABSTRACT This study focused on the detection of the plasmid-mediated mcr colistin resistance gene in Escherichia coli isolates from wastewater treatment plants (WWTPs). Seven influent samples were collected from three WWTPs in Nagano Prefecture, Japan, during August and December 2018. Colistin-resistant E. coli isolates were selected on colistin-supplemented CHROMagar ECC plates. mcr-1-positive isolates were subjected to whole-genome sequencing (WGS) analysis. From six influent samples, seven mcr-1-positive but extended-spectrum β-lactamase (ESBL)-negative isolates belonging to different genetic lineages, namely, B2-O25:H4-ST131-fimH22, B2-O2:H1-ST135-fimH2, B1-O8:H9-ST764-fimH32, B1-O23:H16-ST453-fimH31, A-O81:H27-ST10-fimH54, A-O16:H5-ST871-fimH25, and F-O11:H6-ST457-fimH145, were detected. The MICs of colistin for these isolates ranged from 4 to 16 mg/liter. The mcr-1 genes were located on plasmids belonging to IncX4 and IncI2 in five and two isolates, respectively. Four IncX4 plasmids with the same size (33,309 bp) showed high sequence similarity (4 single-nucleotide variations). The remaining one IncX4 plasmid, with a size of 33,858 bp, carried the mcr-1 gene with the single synonymous nucleic substitution T27C. Two IncI2 plasmids with sizes of 60,710 bp and 60,733 bp had high sequence similarity (99.9% identity; 100% query coverage). Two of five isolates carrying IncX4 plasmids and both of the isolates carrying IncI2 plasmids harbored ColV plasmids carrying virulence-associated genes of avian pathogenic E. coli (APEC). In addition, another isolate of the B2-O25:H4-ST131-fimH22 lineage had those APEC-associated virulence genes on its chromosome. In conclusion, mcr-1-positive E. coli environmental isolates were mostly characterized as positive for APEC-associated virulence genes. The copresence of those genes may suggest the existence of a common source in animals and/or their associated environments. IMPORTANCE Colistin is considered a last-line therapeutic option in severe infections due to multidrug-resistant Gram-negative bacteria, in particular carbapenemase-producing Enterobacteriaceae and multidrug-resistant Acinetobacter baumannii. An increasing prevalence of mcr genes in diverse Enterobacteriaceae species, mainly Escherichia coli and Klebsiella pneumoniae from humans and food animals, has become a significant concern to public health all over the world. In Japan, mcr genes have so far been detected in food animals, raw meat, wastewater, and human clinical samples. This study reports the copresence of mcr-1 and avian pathogenic E. coli (APEC)-associated virulence genes in five of seven E. coli isolates recovered from aquatic environments in Japan. Our study highlights the importance and urgency of action to reduce environmental contamination by mcr genes that may likely occur due to exposure to untreated wastewater through combined sewer overflow by recent unusual weather.

Plasmid Replicons and β-Lactamase-Encoding Genes of Multidrug-Resistant Escherichia coli Isolated from Humans and Food Animals in Lagos, Southwest Nigeria

2019 ◽  
Vol 25 (10) ◽  
pp. 1410-1423 ◽  
Author(s):  
Eyitayo O. Adenipekun ◽  
Charlene R. Jackson ◽  
Hazem Ramadan ◽  
Bamidele A. Iwalokun ◽  
Jonathan G. Frye ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Southwest Nigeria ◽  
Food Animals ◽  
Encoding Genes ◽  
Plasmid Replicons

Prevalence and characterisation of CTX-M β-lactamases amongst Escherichia coli isolates from healthy food animals in China

2012 ◽  
Vol 39 (4) ◽  
pp. 305-310 ◽  
Author(s):  
Hongqing Zheng ◽  
Zhenling Zeng ◽  
Sheng Chen ◽  
Yahong Liu ◽  
Qiongfen Yao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Healthy Food ◽  
Food Animals

scholarly journals Extended-spectrum β-lactamase-producing Escherichia coli isolated from raw vegetables in South Korea

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jihyun Song ◽  
Sung-Suck Oh ◽  
Junghee Kim ◽  
Jinwook Shin
Keyword(s):  
Escherichia Coli ◽  
South Korea ◽  
Vegetable Intake ◽  
Molecular Genetic ◽  
Multidrug Resistant ◽  
E Coli ◽  
Extended Spectrum ◽  
Genetic Features ◽  
Global Concern ◽  
The One

AbstractThe increasing prevalence of oxyimino-cephalosporin-resistant Enterobacteriaceae has become a global concern because of their clinical impact on both human and veterinary medicine. The present study determined the prevalence, antimicrobial susceptibility, and molecular genetic features of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (ESBL-EC) isolates from raw vegetables. A total of 1324 samples were collected from two agricultural wholesale markets in Incheon, South Korea in 2018. The ESBL-EC strains were isolated from 0.83% (11/1324) samples, and all of them were resistant to ampicillin, piperacillin, cefazoline, cefotaxime, and nalidixic acid and yielded CTX-M-type ESBL, including CTX-M-14, CTX-M-15, CTX-M-55, CTX-M-27, and CTX-M-65. The isolates belonged to phylogenetic subgroups D (n = 5), A (n = 4), and B1 (n = 2). Multilocus sequence typing revealed nine known E. coli sequence types (STs), including ST10, ST38, ST69, ST101, ST224, ST349, ST354, ST2509, ST2847, and two new STs. Notably, ST69, ST10, ST38, and ST354 belong to the major human-associated extraintestinal pathogenic E. coli lineages. Our results demonstrate that ESBL-producing multidrug-resistant pathogens may be transmitted to humans through the vegetable intake, highlighting the importance of resistance monitoring and intervention in the One Health perspective.

scholarly journals Genome Sequences of Five Multidrug-Resistant Escherichia coli Sequence Type 117 Isolates Recovered from Dairy Calves

2017 ◽  
Vol 5 (33) ◽  
Author(s):  
Seon-Woo Kim ◽  
Jeffrey S. Karns ◽  
Jo Ann S. Van Kessel ◽  
Bradd J. Haley
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Dairy Calves ◽  
Genome Sequences ◽  
Content Type ◽  
Food Animals ◽  
Tract Infections

ABSTRACT Escherichia coli sequence type 117 (ST117) strains have been recovered from poultry with colibacillosis, as well as from urinary tract infections and fatal septic infections in humans. To further investigate ST117 isolates recovered from nonpoultry food animals, we sequenced the genomes of five ST117 isolates from dairy calves in Pennsylvania.

Antibiotic Resistance of Escherichia coli Isolated from Healthy Food Animals in Kenya

2007 ◽  
Vol 30 (1) ◽  
Author(s):  
I M Mapenay ◽  
G M Kikuvi ◽  
E S Mitema ◽  
J N Ombui
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Healthy Food ◽  
Food Animals
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