An in vitro method for the quantitative determination of the antimicrobial efficacy of silver-containing wound dressings

2009 ◽  
Vol 366 (1-2) ◽  
pp. 111-116 ◽  
Author(s):  
Simon Gaisford ◽  
Anthony E. Beezer ◽  
Alistair H. Bishop ◽  
Michael Walker ◽  
David Parsons
Keyword(s):  
Quantitative Determination ◽  
Wound Dressings ◽  
Antimicrobial Efficacy ◽  
In Vitro Method

AN IN-VITRO METHOD FOR THE DETERMINATION OF MACERATION POTENTIAL OF VARIOUS WOUND DRESSINGS

2003 ◽  
Vol 30 (3) ◽  
pp. S22-S23
Author(s):  
S. M. Adams ◽  
D. C. Pritchard ◽  
B. Griffiths ◽  
S. M. Bishop
Keyword(s):  
Wound Dressings ◽  
In Vitro Method

scholarly journals An in vitro method for the determination of protein turnover in incubated proximal tubule segments

1993 ◽  
Vol 43 (5) ◽  
pp. 1156-1159 ◽  
Author(s):  
Robert May ◽  
Brian Logue ◽  
Byrad Edwards ◽  
Swati Patel
Keyword(s):  
Proximal Tubule ◽  
Protein Turnover ◽  
In Vitro Method ◽  
Proximal Tubule Segments

scholarly journals Development and validation of the method for the quantitative determination of methotrexate in a transport medium by HPLC-MS/MS

2021 ◽  
pp. 45-51
Author(s):  
P. Yu. Mylnikov ◽  
Yu. Tranova ◽  
A. V. Shchulkin ◽  
E. N. Yakusheva
Keyword(s):  
Quantitative Determination ◽  
Gradient Elution ◽  
Sample Volume ◽  
Transport Medium ◽  
Mass Selective Detector ◽  
Transporter Protein ◽  
Wide Range ◽  
Separation Temperature

Relevance. BCRP is an efflux transporter protein that plays an important role in the pharmacokinetics of a wide range of drugs. The BCRP activity in vitro experiments is assessed by the transport of transporter protein substrates (methotrexate, etc.) across the bilipid membrane of cells overexpressingBCRP, for example, Caco-2 cells. The aim is to develop and validate a method for the quantitative determination of the BCRP substrate, methotrexate, in the transport medium of Caco-2 cells by HPLC-MS/MS. Methods. The work was performed on an Ultimate 3000 HPLC chromatograph (ThermoFisher, USA) with a TSQ Fortis tandem mass-selective detector (ThermoFisher, USA). The conditions of chromatographic analysis were as follows: column UCT Selectra C18 4.6 mm * 100 mm 5um, 100A, Selectra C18 Guard Cartridges SLC-18GDC46-5UM, separation temperature 35 °С, flow rate 0.3 ml/min, injected sample volume - 2 μl, analysis time - 10 min. Used a gradient elution: the ratio of the solution of 0.1 % formic acid and acetonitrile was at 0 min 75 and 25 %; 0.4 min 60 and 40 %; 6 minutes 20 and 80 %; 8 minutes 75 and 25 %. Under these conditions, the retention time of methotrexate is 3.11 minutes. Detection conditions: methotrexate - positive ionization mode, 455.15 m / z → 308.125 m / z, collision energy 22.99 V, source fragmentation 5, CID gas pressure 2 mTorr. The extraction of methotrexate from the transport medium (Hanks solution with 25 mM Hepes and 1% dimethyl sulfoxide) after incubation with Caco-2 cells for 3 h was carried out with a mixture of methanol + water in a ratio of 1: 1. Results. The developed method was validated according to the following parameters: selectivity, linearity, accuracy, precision, limit of quantitative determination, sample transfer, sample stability. The confirmed analytical range of the method was 60 -10,000 nmol / L in the transport medium. Conclusions: a method for the quantitative determination of methotrexate in the transport medium of Caco-2 cells by HPLC-MS / MS was developed and validated.

Pegylated magnetic nanocarriers for doxorubicin delivery: A quantitative determination of stealthiness in vitro and in vivo

2012 ◽  
Vol 81 (3) ◽  
pp. 498-505 ◽  
Author(s):  
E. Allard-Vannier ◽  
S. Cohen-Jonathan ◽  
J. Gautier ◽  
K. Hervé-Aubert ◽  
E. Munnier ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Doxorubicin Delivery ◽  
Magnetic Nanocarriers

scholarly journals In vitro evaluation of a manganese chloride phantom-based MRI technique for quantitative determination of lumbar intervertebral disc composition and condition

2010 ◽  
Vol 20 (3) ◽  
pp. 434-439 ◽  
Author(s):  
Lachlan J. Smith ◽  
Andrew P. Kurmis ◽  
John P. Slavotinek ◽  
Nicola L. Fazzalari
Keyword(s):  
Intervertebral Disc ◽  
Quantitative Determination ◽  
Manganese Chloride ◽  
Lumbar Intervertebral Disc ◽  
In Vitro Evaluation

scholarly journals Identification and quantitative determination of pinoresinol in Taxus ×media Rehder needles, cell suspension and shoot cultures

2015 ◽  
Vol 84 (1) ◽  
pp. 125-132 ◽  
Author(s):  
Paulina Mistrzak ◽  
Hanna Celejewska-Marciniak ◽  
Wojciech J. Szypuła ◽  
Olga Olszowska ◽  
Anna K. Kiss
Keyword(s):  
Cell Culture ◽  
Suspension Culture ◽  
Quantitative Determination ◽  
Cell Suspension ◽  
Dry Weight ◽  
Shoot Cultures ◽  
Taxus Media ◽  
In Vitro Shoot Cultures

The aim of our study was to investigate the presence and quantitative contents of lignans in the tissues of <em>Taxus</em> ×<em>media</em>. The presence of the lignans: pinoresinol, matairesinol and secoisolariciresinol was assessed in needles, shoots cultures and suspension culture. Pinoresinol was the only lignan found in the tissue of <em>T.</em> ×<em>media</em>. The total pinoresinol content in the needles and in the shoots was 1.24 mg/g dry weight (dw) and 0.69 mg/g dw, respectively. Most of the pinoresinol identified was appeared glycosidically bound. In needles, the amount of glycosidically bound pinoresinol (0.81 mg/g dw) was about twice as high as that of free pinoresinol (0.43 mg/g dw). The content of free and glycosidically bound pinoresinol showed the level of 0.18 mg/g dw and 0.51 mg/g dw, respectively in the in vitro shoot cultures. In the cell culture, no pinoresinol was found.

scholarly journals In vitro evaluation of immunogenic properties of active dressings

2014 ◽  
Vol 27 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Joanna Orlowska ◽  
Urszula Kurczewska ◽  
Katarzyna Derwinska ◽  
Wojciech Orlowski ◽  
Daria Orszulak-Michalak
Keyword(s):  
Immune Response ◽  
Wound Dressings ◽  
Enzyme Linked Immunosorbent Assay ◽  
In Vitro Evaluation ◽  
Murine Fibroblasts ◽  
The Subject ◽  
Immunogenic Properties

Abstract The aim of this study was in vitro evaluation of the level of the immune response in relation to wound dressings composed of alginate, calcium carboxymethylcellulose, and dibutyrylochitin and determination of the direction of response, which will make referring next to the results of in vivo phase possible. The subject of the experiments was to examine the commercially available, biodegradable alginate dressing, commercially available but not biodegradable dressing constructed from the sodium carboxymethylcellulose, and synthesized in house biodegradable dressing constructed of the dibutyrylchitin. To determine the direction of the immune response, the degree of secretion of pro-inflammatory interleukin (IL-1, IL-6) and antiinflammatory (IL-10) interleukin from murine fibroblasts having contact with the tested dressings (ELISA enzyme linked immunosorbent assay), was tested.

scholarly journals Development and validation of a method for the determination of the specific activity of recombinant monoclonal antibody eculizumab

2020 ◽  
Vol 15 (2) ◽  
pp. 77-85
Author(s):  
D. I. Zybin ◽  
A. S. Seregin ◽  
A. D. Askretkov ◽  
N. V. Orlova ◽  
Yu. A. Seregin ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Pharmaceutical Analysis ◽  
Comparative Evaluation ◽  
Specific Activity ◽  
In Vitro Method ◽  
Recombinant Monoclonal Antibody ◽  
Development And Validation ◽  
First Time

Objectives. Developing reliable and accurate analytical methods is necessary for comparative pharmaceutical analysis using physicochemical, biological (in vitro), preclinical, and clinical trials. The main objective of this study was to develop and validate an in vitro method for determining the specific activity of the recombinant monoclonal antibody eculizumab.Methods. The method of indirect enzyme immunoassay was used in the study.Results. A method for determining the specific activity of the humanized recombinant monoclonal antibody eculizumab was described and validated for the first time. A comparative evaluation of the specific activity of Soliris® (Alexion Pharmaceuticals Inc., USA), and its biosimilar PRK-001 (Pharmapark, Russia) was performed using the developed method.Conclusions. The similarity of PRK-001 and the original Soliris® in relation to their specific activity, that is, binding to the human complement system C5 protein, was proved. 

Qualitative and Quantitative Determination of Quorum Sensing Inhibition In Vitro

2017 ◽  
pp. 275-285 ◽  
Author(s):  
Tim Holm Jakobsen ◽  
Maria Alhede ◽  
Louise Dahl Hultqvist ◽  
Thomas Bjarnsholt ◽  
Michael Givskov
Keyword(s):  
Quorum Sensing ◽  
Quantitative Determination ◽  
Quorum Sensing Inhibition ◽  
Qualitative And Quantitative
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