Formulation and in vitro characterization of inhalable polyvinyl alcohol-free rifampicin-loaded PLGA microspheres prepared with sucrose palmitate as stabilizer: Efficiency for ex vivo alveolar macrophage targeting

2012 ◽  
Vol 436 (1-2) ◽  
pp. 833-839 ◽  
Author(s):  
R. Diab ◽  
J. Brillault ◽  
A. Bardy ◽  
A.V.L. Gontijo ◽  
J.C. Olivier
Keyword(s):  
Polyvinyl Alcohol ◽  
Alveolar Macrophage ◽  
Ex Vivo ◽  
Plga Microspheres ◽  
In Vitro Characterization ◽  
Macrophage Targeting

In vitro characterization of polyvinyl alcohol assisted hydroxyapatite derived by sol–gel method

2014 ◽  
Vol 35 ◽  
pp. 239-244 ◽  
Author(s):  
Omer Kaygili ◽  
Serhat Keser ◽  
R.H. Al Orainy ◽  
Tankut Ates ◽  
Fahrettin Yakuphanoglu
Keyword(s):  
Polyvinyl Alcohol ◽  
Sol Gel ◽  
Gel Method ◽  
Sol Gel Method ◽  
In Vitro Characterization

scholarly journals Comparative characterization of the reassortant Orthobunyavirus Ngari with putative parental viruses, Bunyamwera and Batai: in vitro characterization and ex vivo stability

2020 ◽  
Author(s):  
M. Fausta Dutuze ◽  
E. Handly Mayton ◽  
Joshua D. Macaluso ◽  
Rebecca C. Christofferson
Keyword(s):  
Ex Vivo ◽  
Laboratory System ◽  
In Vitro Characterization ◽  
Significant Difference ◽  
The Relationship ◽  
Kinetics Of ◽  
Negative Sense

Bunyamwera (BUNV), Batai (BATV) and Ngari (NRIV) are mosquito-borne viruses that are members of the genus Orthobunyavirus in the order Bunyavirales. These three viruses are enveloped with single-stranded, negative-sense RNA genomes consiting of three segments, denoted as Small (S), Medium (M) and Large (L). Ngari is thought to be the natural reassortant progeny of Bunyamwera and Batai viruses. The relationship between these ‘parental’ viruses and the ‘progeny’ poses an interesting question, especially given that there is overlap in their respective transmission ecologies, but differences in their infection host ranges and pathogenesis. We compared the in vivo kinetics of these three viruses in a common laboratory system and found no significant difference in growth kinetics. There was, however, a tendency of BATV to have smaller plaques than either BUNV or NRIV. Furthermore, we determined that all three viruses are stable in extracellular conditions and retain infectivity for a week in non-cellular media, which has public health and biosafety implications. The study of this understudied group of viruses addresses a need for basic characterization of viruses that have not yet reached epidemic transmission intensity, but that have the potential due to their infectivity to both human and animal hosts. These results lay the groundwork for future studies of these neglected viruses of potential public and One Health importance.

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