Characterization of avian thymic hormone and chicken parvalbumin 3 target cells

2013 ◽  
Vol 15 (2) ◽  
pp. 282-288
Author(s):  
Renata Novak Kujundžić ◽  
Walstine L. Steffens ◽  
John M. Brewer ◽  
Michael T. Henzl ◽  
William L. Ragland
Keyword(s):  
Target Cells ◽  
Thymic Hormone

scholarly journals Cell surface phenotyping of megakaryoblasts

Blood ◽  
1987 ◽  
Vol 69 (3) ◽  
pp. 957-960 ◽  
Author(s):  
T Koike ◽  
S Aoki ◽  
S Maruyama ◽  
M Narita ◽  
T Ishizuka ◽  
...  
Keyword(s):  
Cell Surface ◽  
Acute Leukemia ◽  
Peroxidase Activity ◽  
Down’S Syndrome ◽  
Phenotypic Characterization ◽  
Target Cells ◽  
Monocytic Cells ◽  
Transient Abnormal Myelopoiesis ◽  
Hla Dr

Abstract Surface phenotypic characterization of megakaryoblasts, identified by platelet peroxidase activity, was investigated in four patients who showed increased proliferation of megakaryoblasts: one patient with typical features of acute leukemia, one presenting with acute myelofibrosis, and two with Down's syndrome in whom blasts disappeared spontaneously (transient abnormal myelopoiesis, TAM). MY10 and/or MY9 antigens were expressed on the surface of some megakaryoblasts, but MY7, and MY4, antigens specific to granulocytic or monocytic cells, were not. Some megakaryoblasts were positive for only anti-HLA-DR antibodies. It was speculated that, during the differentiation of the megakaryocytic lineage, MY9 antigen appears transiently on the surface of megakaryoblasts that have lost HLA-DR antigens and have gained the glycoprotein IIb/IIIa antigen. This study also demonstrated that the proliferating blasts in some patients with TAM were mainly megakaryoblasts and suggested that the target cells in TAM are CFU-GEMM.

scholarly journals Functional characterization of a stable, noncytolytic stage of macrophage activation in tumors.

1977 ◽  
Vol 146 (6) ◽  
pp. 1511-1520 ◽  
Author(s):  
S W Russell ◽  
W F Doe ◽  
A T McIntosh
Keyword(s):  
Macrophage Activation ◽  
Functional Characterization ◽  
Cytolytic Activity ◽  
Target Cells ◽  
Tumor Target ◽  
Total Absence

The state in which macrophages (Mphi) from regressing Moloney sarcomas could kill tumor target cells was a highly labile one which decayed rapidly in vitro. Thereafter, regressor Mphi were noncytolytic. Mphi from several different progressing sarcomas failed to kill, even when challenged with target cells immediately after explantation. Similarly, thioglycollate-induced peritoneal Mphi (TG-Mphi) did not kill. Noncytolygic Mphi derived either from progressing sarcomas or from long-term (up to 96 h) cultures of regressor Mphi were exquisitely sensitive to stimulation by bacterial lipopolysaccharide (LPS); picogram/milliliter amounts induced killing. Similar concentrations of LPS had no demonstrable effect on TG-Mphi. Thus, tumor Mphi generally appeared to have been primed in vivo, with those in regressing sarcomas having additionally acquired cytolytic activity. Inability of progressor Mphi to kill apparently stemmed from lack of, or failure to respond to, the signal needed in vivo to trigger cytolytic activity, rather than the total absence of activation.

Characterization of Nanoparticle Distribution in Microcirculation Through a Microfluidics Device

2011 ◽  
Author(s):  
Antony Thomas ◽  
Jifu Tan ◽  
Susan Perry ◽  
Yaling Liu
Keyword(s):  
Drug Delivery ◽  
Drug Targeting ◽  
Volume Ratio ◽  
Target Cells ◽  
Nanoparticle Distribution ◽  
Nano Drug Delivery ◽  
Targeting Delivery ◽  
Drug Doses ◽  
Lower Drug

Various methods of targeted nano drug delivery have been developed in recent years to reduce side effects, toxicity, and lower drug doses [1]. The use of nanoparticles in drug delivery provides advantages in drug targeting, delivery and release along with serving in diagnosis and therapy [2]. Higher percentage of nanoparticle drug is uptaken by the target cells while larger drug particles are easily cleaned off by the human body. Nanoparticles also have large surface to volume ratio, which aids in attachment of many functional groups and thereby enhances targeting.

scholarly journals STUDIES ON CHARACTERIZATION OF THE LYMPHOID TARGET CELL FOR ACTIVITY OF A THYMUS HUMORAL FACTOR

1973 ◽  
Vol 138 (1) ◽  
pp. 130-142 ◽  
Author(s):  
Varda Rotter ◽  
Amiela Globerson ◽  
Ichiro Nakamura ◽  
Nathan Trainin
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Cell Activity ◽  
Humoral Factor ◽  
Target Cells ◽  
Total Body ◽  
Thymus Cells ◽  
Marrow Cells

The immune response to SRBC was measured in the spleens of adult thymectomized, total body irradiated mice injected with various combinations of thymus and bone marrow cells together with thymic humoral factor (THF). It was found that the number of plaque-forming cells was significantly increased when THF was given in vivo immediately after thymus cell administration or when thymus cells were incubated in THF before injection. On the other hand, bone marrow cells equally treated did not manifest any T cell activity, since THF-treated bone marrow cells were not able to substitute thymus cells in the system used. The results accumulated in the present experiments indicate, therefore, that the target cells for THF activity are thymus cells which acquire a higher T helper cell capacity after THF treatment.

scholarly journals Ultrastructural study of spontaneous bone marrow rosette-forming cells.

1977 ◽  
Vol 72 (3) ◽  
pp. 773-777 ◽  
Author(s):  
B Nabarra ◽  
J Charreire ◽  
J F Cavellier ◽  
J F Bach
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Bone Marrow ◽  
T Cell ◽  
Ultrastructural Study ◽  
Target Cells ◽  
Mouse Bone Marrow ◽  
Thymic Hormone ◽  
Mouse Lymphocytes ◽  
Scanning Electron

Mouse bone marrow contains spontaneous rosette-forming cells (RFC) which include more than 70% T-cell precursors, as assessed by their transformation into theta-positive cells after incubation with thymic hormone. Such spontaneous RFC, examined in C57B1/6 mouse bone marrow by electron and scanning electron microscopy, have consistently been shown to be small, inactive mouse lymphocytes when macrophages have been eliminated by cell preincubation. These data suggest that thymic hormone target cells include small quiescent lymphocytes.

scholarly journals Semliki Forest virus-derived virus-like particles: characterization of their production and transduction pathways

2005 ◽  
Vol 86 (11) ◽  
pp. 3129-3136 ◽  
Author(s):  
A. Diatta ◽  
E. Piver ◽  
C. Collin ◽  
P. Vaudin ◽  
J.-C. Pagès
Keyword(s):  
Molecular Mechanisms ◽  
Semliki Forest Virus ◽  
Target Cells ◽  
Entry Site ◽  
Internal Ribosome Entry ◽  
Replication Complex ◽  
Virus Like Particles ◽  
Vesicular Stomatitis ◽  
Viral Envelopes

A procedure for the mobilization of Semliki Forest virus (SFV)-derived replicons using virus-like particles (VLPs) has been recently proposed. VLPs were obtained from 293T cells co-expressing the vesicular stomatitis virus glycoprotein (VSV-G) and a modified SFV replicon. Advantages of SFV VLPs include improved safety with a lack of sequence homology between components and reducing the risk of recombination events that could lead to the formation of autonomous particles. Characterization of SFV VLPs reveals a discrepancy in their ability to infect cells reported to be permissive. Furthermore, it was noted that not all viral envelopes were able to promote VLP release equally from transfected cells. These observations encouraged the examination of the molecular mechanisms supporting the different steps of VLP assembly and transduction. The use of a VSV-G related pathway for VLP entry into target cells was demonstrated; it was also observed that an internal ribosome entry site may not be adapted to control transgene expression in all cells. Finally, the need for a membrane-binding domain to obtain a fully active SFV replication complex and VLP formation was documented.

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