In vitro effect of seven essential oils on the reproduction of the cattle tick Rhipicephalus microplus

The objectives of this work were to assess the in vitro effect of essential oils extracted from cinnamon, citronella, lemon grass, India clove, tea tree, thyme, neem and eucalyptus on the conidia germination and on mycelial growth of Cercospora coffeicola, and their efficacy to control the brown eye spot in coffee seedlings (cultivars Catucaí 2SL, Catuaí IAC 62 and Mundo Novo 379/19) in a greenhouse, as well as their effects on the initial germination and infection events by scanning electron microscopy. All essential oils promoted the inhibition of conidia germination with increasing concentrations. India clove, cinnamon, neem, thyme and lemon grass oils inhibited the mycelial growth of C. coffeicola. The cinnamon and citronella oils were the most promising for brown eye spot control in all cultivars. In scanning electron microscopy, the cinnamon and citronella oils reduced germination and mycelial development of C. coffeicola in vivo, eight and 16 hours after inoculation, promoting, in some cases, the leakage of the cellular content. Essential oils of cinnamon and citronella reduced the incidence and severity of brown eye spot, in addition to presenting direct toxicity to the pathogen.

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Baccharis dracunculifolia DC (Asteraceae) leaf and flower essential oils to control Rhipicephalus microplus Canestrini (Arachnida: Ixodidae) in the free-living stage

Research Society and Development ◽

10.33448/rsd-v9i10.8788 ◽

2020 ◽

Vol 9 (10) ◽

pp. e5049108788

Author(s):

Luciane Neris Cazella ◽

Herika Line de Marko de Oliveira ◽

Wanessa de Campos Bortolucci ◽

Isabelle Luiz Rahal ◽

Irinéia Paulina Baretta ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Biological Activities ◽

Probit Analysis ◽

Rhipicephalus Microplus ◽

Acaricidal Activity ◽

Egg Laying ◽

Ex Situ ◽

Egg Hatchability

Baccharis dracunculifolia, native to Brazil and the main source of “green propolis”, has been reported with several biological activities, and may be a source of bovine tick control substituting synthetic acaricides. Objective: to evaluate the in vitro and ex situ acaricidal activity of B. dracunculifolia leaf and flower essential oils against Rhipicephalus microplus. Methodology: the essential oils were extracted by hydrodistillation and analyzed by a gas chromatography coupled to mass spectrometry; the acaricidal activity of the essential oil was evaluated in vitro against adult females and against the egg hatchability; moreover, the acaricidal activity against tick larvae was evaluated in vitro and ex situ. Results: the major class of the essential oils was oxygenated sesquiterpene (55.1% leaves 50.4% flowers) and the main compounds were (21.5% leaves; 20.6% flowers) and spathulenol (21.8% leaves; 20.3% flowers). The essential oil at 500 mg/mL was effective to control egg hatchability with a reduction of egg laying capacity and decrease of number of adult ticks and larvae. The larvicidal activity of the essential oil had LC99.9 from 35 to 37 mg/mL by probit analysis, and the essential oil from 11 to 14 mg/mL presented 85 to 95% of treatment efficiency in the ex situ test. Conclusion: B. dracunculifolia leaf and flower essential oils are stable and have application potential to control bovine ticks.

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Comparison of Eucalyptus globulus and Origanum vulgare essential oils’ in vitro effect on Candida albicans isolated from a vulvo vaginal candidiasis patient

Revista Médica de Trujillo ◽

10.17268/rmt.2020.v15i01.03 ◽

2020 ◽

Vol 15 (1) ◽

pp. 11-25

Author(s):

Olga Durango-Chávez ◽

Elva Mejía-Delgado

Keyword(s):

Candida Albicans ◽

Essential Oils ◽

Eucalyptus Globulus ◽

Vaginal Candidiasis ◽

Origanum Vulgare ◽

Vitro Effect

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Effects of essential oils from thyme, cinnamon and clove on mycelial growth of Colletotrichum acutatum

Pesticidi i fitomedicina ◽

10.2298/pif1002151d ◽

2010 ◽

Vol 25 (2) ◽

pp. 151-156 ◽

Cited By ~ 1

Author(s):

Natasa Duduk ◽

Aleksa Obradovic ◽

Mirko Ivanovic

Keyword(s):

Essential Oils ◽

Mycelial Growth ◽

Petri Dish ◽

Control Treatment ◽

Colletotrichum Acutatum ◽

Dependent Manner ◽

Cinnamon Oil ◽

Volatile Phase ◽

Vitro Effect

Effects of the volatile phase of thyme, cinnamon and clove essential oils on Colletotrichum acutatum were investigated. Mycelial disc was placed in the center of the Petri dish (V=66 ml) containing PDA. Different volumes of either non- or ethanol-diluted essential oils were placed on the inner side of the dish cover to obtain final concentrations of 153, 107, 76, 46, 15, 14, 12, 11, 7.6, 3.82, 1.53, 0.153 and 0.0153 ?l/L of air. The dishes were sealed with Parafilm and incubated in up-side-down position. After 7 days of incubation, mycelial growth was recorded by measuring the colony diameter. If no mycelial growth was recorded, the disc was transferred to a new PDA plate in order to evaluate whether the activity was either fungistatic or fungicidal. Mean growth values were obtained and then converted to inhibition percentage of mycelial growth compared with the control treatment. All the tested essential oils inhibited mycelial growth of C. acutatum in the dose dependent manner. Mycelial growth was totally inhibited by thyme oil in the concentration of 76 ?l/L of air. The same results were obtained by cinnamon and clove oil in the concentration of 107 ?l/L of air. Thyme and cinnamon oil had fungicidal effect in concentrations of 107 and 153 ?l/L respectively. The results obtained provide evidence on the antifungal in vitro effect of the tested essential oils as potential means for the control of C. acutatum.

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Effect of Different Terpene-Containing Essential Oils on the Proliferation ofEchinococcus granulosusLarval Cells

Interdisciplinary Perspectives on Infectious Diseases ◽

10.1155/2014/746931 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 7

Author(s):

Clara María Albani ◽

Guillermo María Denegri ◽

María Celina Elissondo

Keyword(s):

Essential Oils ◽

Effective Means ◽

Control Cell ◽

Larval Growth ◽

Public Health Problem ◽

Mentha Piperita ◽

Major Public Health Problem ◽

Isolated Cells ◽

Vitro Effect

Human cystic echinococcosis remains a major public health problem on several countries and the treatment strategies are not solved. The aim of the present work was to determine the in vitro effect of thymol andMentha piperita,M. pulegium,andRosmarinus officinalisessential oils on the proliferation ofE. granulosuslarval cells. Isolated cells and cellular aggregates were obtained from hydatid cyst’s germinal layer and exposed to 1, 5, and 10 μg/ml of thymol and the different essential oils for 7 days. Drug effect was evaluated using test viability and scanning electron microscopy. Control cell culture viability was 2.1 x 106(100%) after 7 days of incubation. At day 7, thymol 5 μg/ml caused a reduction in cell viability of 63% and the essential oils ofM. piperita10 μg/ml,M. pulegium10 μg/ml, andR. officinalis10 μg/ml produced a reduction in the viability of 77, 82, and 71%, respectively. Moreover essential oils caused reduction in cell number, collapsed cells, and loss of normal tridimensional composition of the aggregates. Due to the inhibitory effect caused by essential oils onE. granulosuscells we suggested that it would be an effective means for suppression of larval growth.

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In Vitro Evaluation of Ethanolic Extracts ofAgeratum conyzoidesandArtemisia absinthiumagainst Cattle Tick,Rhipicephalus microplus

The Scientific World JOURNAL ◽

10.1155/2014/858973 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 8

Author(s):

S. Parveen ◽

R. Godara ◽

R. Katoch ◽

A. Yadav ◽

P. K. Verma ◽

...

Keyword(s):

Immersion Test ◽

Rhipicephalus Microplus ◽

Acaricidal Activity ◽

Cattle Tick ◽

Egg Mass ◽

Ageratum Conyzoides ◽

Artemisia Absinthium ◽

Aerial Parts ◽

Ethanolic Extracts

In vitro efficacy of ethanolic extracts obtained from the aerial parts ofAgeratum conyzoidesandArtemisia absinthiumwas assessed onRhipicephalus microplususing adult immersion test (AIT). Five concentrations of the extract (1.25%, 2.5%, 5%, 10%, and 20%) with three replications for each concentration were used in the bioassay. In AIT, the maximum mortality was recorded as 40% and 66.7% at 20% concentration forA. conyzoidesandA. absinthium, respectively. Acaricidal activity was found to be higher in the extract ofA. absinthiumwith LC50and LC95values of 11.2% and 61.7%, respectively. Egg mass weight of the live ticks treated with different concentrations of the extracts was significantly(P<0.05)lower than that of control ticks; consequently, the reproductive index and oviposition values of the treated ticks were reduced significantly(P<0.05). TheA. conyzoidesinhibited 90% hatching of eggs at the 20% concentration, whereasA. absinthiumshowed 100% inhibition at 5%, 10%, and 20% concentrations. The results show thatA. absinthiumhas better acaricidal properties thanA. conyzoidesand could be useful in controllingR. microplus.

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In vitro Effect of Essential Oils on Rumen Fermentation and Microbial Nitrogen Yield of High Concentrate Dairy Cow Diet

Biosciences Biotechnology Research Asia ◽

10.13005/bbra/2749 ◽

2016 ◽

Vol 16 (2) ◽

pp. 333-341

Author(s):

Seyed Masoud Davoodi ◽

Mohsen Danesh Mesgaran ◽

Ali Reza Vakili ◽

Reza Valizadeh ◽

Abdollah Ghasemi Pirbalouti

Keyword(s):

Essential Oils ◽

Dairy Cow ◽

Dry Matter ◽

Basal Diet ◽

Gas Production ◽

Ruminal Fermentation ◽

Plant Essential Oils ◽

Microbial Nitrogen ◽

Vitro Effect

Present study was conducted to investigate the effect of including plant essential oils on in vitro ruminal fermentation and microbial nitrogen synthesis of a dairy cow diet rich in concentrate. The treatments consisted of the diet alone (control; BD) as well as containing 50 and 100 μl L-1 essential oil of thyme (BDT), mint (BDM), savory (BDS), or a mixture of the essential oils at the rate of 1:1:1 (BDmix). Essential oils decreased gas production at 24, 48 and 96 h of incubation compared with that of BD. However, mint at the rate of 50 or 100 μl L-1 resulted an increase in the microbial nitrogen when compared to BD, BDS and BDT. The nitrogen content of truly undegraded residu (NDFN) content and NH3-N concentration were lower, while the dry matter digestibility was greater in the BDmix, regardless of dosage levels, as compared with the control. The inclusion of a mixture of essential oils at 50 μl L-1 to the basal diet caused intensified dry matter disappearance, in comparison to other treatments. Results showed that the synergetic effects of essential oils together in a dairy cow diet of rich in concentrate can alter rumen microbial fermentation and improve microbial protein yield.

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In vitro effect of essential oils from aromatic and medicinal plants on mushroom pathogens: Verticillium fungicola var. fungicola, Mycogone perniciosa, and Cladobotryum sp.

Archives of Biological Sciences ◽

10.2298/abs0902231t ◽

2009 ◽

Vol 61 (2) ◽

pp. 231-237 ◽

Cited By ~ 13

Author(s):

Brankica Tanovic ◽

Ivana Potocnik ◽

G. Delibasic ◽

M. Ristic ◽

M. Kostic ◽

...

Keyword(s):

Medicinal Plants ◽

Essential Oils ◽

Fungicidal Effect ◽

Verticillium Fungicola ◽

Aromatic And Medicinal Plants ◽

Volatile Phase ◽

Main Components ◽

Oregano Oil ◽

Vitro Effect

Lavender, anise, chamomile, fennel, geranium, oregano, parsley, and sage essential oils were tested for their effectiveness against mushroom pathogens: Verticillium fungicola var. fungicola, Mycogone perniciosa, and Cladobotryum sp. Isolates were exposed to the volatile phase of the oils and then ventilated in order to determine if the effect of the oil was lethal to the pathogen. Oregano and geranium oils were the most toxic, having a fungicidal effect at 0.02-0.08 ?l/ml of air, depending on the pathogen. Oregano oil was characterized by high content of carvacrol and thymol, while citranelol and geraniol were the main components of geranium oil.

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