A comparative study of the fragmentation of neutral lactooligosaccharides in negative-ion mode by UV-MALDI-TOF and UV-MALDI ion-trap/TOF mass spectrometry

<p>Reactions carried out in the presence of rubber septa run the risk of additives being leached out by the solvent. Normally, such species are present at low enough levels that they do not interfere with the reaction significantly. However, when studying reactions using sensitive methods such as mass spectrometry, the appearance of even trace amounts of material can confuse dynamic analyses of reactions. A wide variety of additives are present in rubber along with the polymer: antioxidants, dyes, detergent, and vulcanization agents, and these are all especially problematic in negative ion mode. A redesigned Schlenk flask for pressurized sample infusion (PSI) is presented as a means of practically eliminating the presence of contaminants during reaction analyses.</p>

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Identification of carbonylated peptides by tandem mass spectrometry using a precursor ion-like scan in negative ion mode

Journal of Proteomics ◽

10.1016/j.jprot.2011.05.033 ◽

2011 ◽

Vol 74 (11) ◽

pp. 2351-2359 ◽

Cited By ~ 23

Author(s):

Ravi Ch. Bollineni ◽

Maria Fedorova ◽

Ralf Hoffmann

Keyword(s):

Mass Spectrometry ◽

Tandem Mass Spectrometry ◽

Negative Ion ◽

Tandem Mass ◽

Negative Ion Mode

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LC-MS Analysis of Low Molecular Weight Carbonyl Compounds as 2,4-Dinitrophenylhydrazones Using Negative Ion Mode Electronspray Ionization Mass Spectrometry

Journal of Chromatography & Separation Techniques ◽

10.4172/2157-7064.1000108 ◽

2011 ◽

Vol 02 (01) ◽

Cited By ~ 2

Author(s):

Akio Yasuhara ◽

Yuuka Tanaka ◽

Miyuki Makishima ◽

Shigeru Suzuki ◽

Takayuki Shibamoto

Keyword(s):

Mass Spectrometry ◽

Molecular Weight ◽

Carbonyl Compounds ◽

Negative Ion ◽

Ionization Mass Spectrometry ◽

Low Molecular Weight ◽

Ionization Mass ◽

Ms Analysis ◽

Negative Ion Mode

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Investigation of electrospray for a capillary electrophoresis–mass spectrometry interface in reverse polarity and negative ion mode

European Journal of Mass Spectrometry ◽

10.1177/1469066719828192 ◽

2019 ◽

Vol 25 (1) ◽

pp. 157-163 ◽

Cited By ~ 1

Author(s):

Morgan Stickney ◽

Qiangwei Xia ◽

I Jonathan Amster

Keyword(s):

Mass Spectrometry ◽

Electroosmotic Flow ◽

Negative Ion ◽

Reverse Polarity ◽

Sheath Flow ◽

Analytical Technique ◽

Sheath Liquid ◽

Electrospray Process ◽

Negative Ionization ◽

Negative Ion Mode

Capillary zone electrophoresis (CZE) paired with mass spectrometry (MS) is a powerful analytical technique for examining mixtures of ionic analytes such as glycosaminoglycans. This study examines the mechanics of the electrospray process for a sheath flow CZE-MS interface under reverse polarity negative ionization conditions. Liquid flow in a sheath flow nano-electrospray CZE-MS interface is driven by two mechanisms, electroosmotic flow and electrospray nebulization. The contribution of these two processes to the overall flow of solution to the electrospray tip is influenced by the surface coatings of the sheath flow emitter tip and by the solvent composition. We have investigated the application of this interface to the reverse polarity separation of glycosaminoglycans and find that the role of electroosmotic flow is far less than has been reported previously, and the electrospray process itself is the largest contributor to the flow of the sheath liquid.

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