scholarly journals Bacterial pyruvate production from alginate, a promising carbon source from marine brown macroalgae

2014 ◽  
Vol 117 (3) ◽  
pp. 269-274 ◽  
Author(s):  
Shigeyuki Kawai ◽  
Kazuto Ohashi ◽  
Shiori Yoshida ◽  
Mari Fujii ◽  
Shinichi Mikami ◽  
...  
Keyword(s):  
Carbon Source ◽  
Brown Macroalgae

scholarly journals 4-Deoxy-l-erythro-5-hexoseulose Uronate (DEH) and DEH Reductase: Key Molecule and Enzyme for the Metabolism and Utilization of Alginate

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 338
Author(s):  
Shigeyuki Kawai ◽  
Wataru Hashimoto
Keyword(s):  
Carbon Source ◽  
Molecular Identification ◽  
Alginate Lyase ◽  
Amino Groups ◽  
Structural Determinants ◽  
Brown Macroalgae ◽  
Practical Utilization ◽  
Coenzyme Specificity ◽  
Enzymatic Formation ◽  
Classification Function

4-Deoxy-l-erythro-5-hexoseulose uronate (DEH), DEH reductase, and alginate lyase have key roles in the metabolism of alginate, a promising carbon source in brown macroalgae for biorefinery. In contrast to the widely reviewed alginate lyase, DEH and DEH reductase have not been previously reviewed. Here, we summarize the current understanding of DEH and DEH reductase, with emphasis on (i) the non-enzymatic and enzymatic formation and structure of DEH and its reactivity to specific amino groups, (ii) the molecular identification, classification, function, and structure, as well as the structural determinants for coenzyme specificity of DEH reductase, and (iii) the significance of DEH for biorefinery. Improved understanding of this and related fields should lead to the practical utilization of alginate for biorefinery.

scholarly journals Metabolomic and Transcriptomic Analyses of Escherichia coli for Efficient Fermentation of L-Fucose

Marine Drugs ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. 82 ◽  
Author(s):  
Jungyeon Kim ◽  
Yu Cheong ◽  
Inho Jung ◽  
Kyoung Kim
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Carbon Source ◽  
Large Scale ◽  
Cellular Metabolism ◽  
Scale Production ◽  
Brown Macroalgae ◽  
E Coli ◽  
Biological Interpretation ◽  
Efficient Fermentation

L-Fucose, one of the major monomeric sugars in brown algae, possesses high potential for use in the large-scale production of bio-based products. Although fucose catabolic pathways have been enzymatically evaluated, the effects of fucose as a carbon source on intracellular metabolism in industrial microorganisms such as Escherichia coli are still not identified. To elucidate the effects of fucose on cellular metabolism and to find clues for efficient conversion of fucose into bio-based products, comparative metabolomic and transcriptomic analyses were performed on E. coli on L-fucose and on D-glucose as a control. When fucose was the carbon source for E. coli, integration of the two omics analyses revealed that excess gluconeogenesis and quorum sensing led to severe depletion of ATP, resulting in accumulation and export of fucose extracellularly. Therefore, metabolic engineering and optimization are needed for E. coil to more efficiently ferment fucose. This is the first multi-omics study investigating the effects of fucose on cellular metabolism in E. coli. These omics data and their biological interpretation could be used to assist metabolic engineering of E. coli producing bio-based products using fucose-containing brown macroalgae.

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

Brown macroalgae produce anti-leukemia compounds

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
C Vizetto-Duarte ◽  
D Santos ◽  
L Custódio ◽  
L Barreira ◽  
H Pereira ◽  
...  
Keyword(s):  
Brown Macroalgae

Enantioselective Multi-Component Cyclopropane Synthesis Enabled by Cu-Catalyzed Cyclopropene Carbometallation with Organoboron Reagent

2018 ◽  
Author(s):  
Zhanyu Li ◽  
Mengru Zhang ◽  
Yu Zhang ◽  
Shuang Liu ◽  
Jinbo Zhao ◽  
...  
Keyword(s):  
Carbon Source ◽  
Stereoselective Synthesis ◽  
Organometallic Reagents ◽  
Preliminary Results ◽  
Quaternary Center ◽  
Chiral Centers ◽  
Substituted Cyclopropanes ◽  
Proof Of Principle

Deployment of organoboron in lieu of the strongly basic <br>organometallic reagents as carbon source in Cu-catalyzed <br>cyclopropene carbometallation opens unprecedented three-<br>component reactivity for stereoselective synthesis of poly-substituted cyclopropanes. A proof-of-principle demonstration of this novel carbometallation strategy is presented herein for a highly convergent access to poly-substituted aminocyclopropane framework via <br>carboamination. Preliminary results on asymmetric desymmetrization with commercial bisphosphine ligands attained high levels of enantioselection, offering a straightforward access to enantioenriched aminocyclopropanes bearing all-three chiral centers, including an all-carbon quaternary center. This strategy may underpin a host of novel synthetic protocols for poly-substituted cyclopropanes. <br>

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