Relationships among lipid peroxidation, enzyme activity and gene expression profiles of superoxide dismutase in Lycopersicum esculentum L. exposed to cold stress

2012 ◽  
Vol 161 ◽  
pp. 47
Author(s):  
Semra Soydam Aydin ◽  
Esra Gokce ◽  
Sumer Aras
Keyword(s):  
Gene Expression ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Cold Stress ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Lycopersicum Esculentum

scholarly journals Gene Expression Profiles Suggest a Better Cold Acclimation of Polyploids in the Alpine Species Ranunculus kuepferi (Ranunculaceae)

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1818
Author(s):  
Eleni Syngelaki ◽  
Claudia Paetzold ◽  
Elvira Hörandl
Keyword(s):  
Gene Expression ◽  
Cold Stress ◽  
Cold Acclimation ◽  
Temperature Stress ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Distribution Patterns ◽  
Gene Set ◽  
Cation Homeostasis ◽  
Response To Temperature

Alpine habitats are shaped by harsh abiotic conditions and cold climates. Temperature stress can affect phenotypic plasticity, reproduction, and epigenetic profiles, which may affect acclimation and adaptation. Distribution patterns suggest that polyploidy seems to be advantageous under cold conditions. Nevertheless, whether temperature stress can induce gene expression changes in different cytotypes, and how the response is initialized through gene set pathways and epigenetic control remain vague for non-model plants. The perennial alpine plant Ranunculus kuepferi was used to investigate the effect of cold stress on gene expression profiles. Diploid and autotetraploid individuals were exposed to cold and warm conditions in climate growth chambers and analyzed via transcriptome sequencing and qRT-PCR. Overall, cold stress changed gene expression profiles of both cytotypes and induced cold acclimation. Diploids changed more gene set pathways than tetraploids, and suppressed pathways involved in ion/cation homeostasis. Tetraploids mostly activated gene set pathways related to cell wall and plasma membrane. An epigenetic background for gene regulation in response to temperature conditions is indicated. Results suggest that perennial alpine plants can respond to temperature extremes via altered gene expression. Tetraploids are better acclimated to cold conditions, enabling them to colonize colder climatic areas in the Alps.

scholarly journals Regulatory networks of gene expression in maize (Zea mays) under drought stress and re-watering

2018 ◽  
Author(s):  
Liru Cao ◽  
Xiaomin Lu ◽  
Pengyu Zhang ◽  
Lixia Ku ◽  
Guorui Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Superoxide Dismutase ◽  
Abscisic Acid ◽  
Drought Stress ◽  
Stomatal Conductance ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Proline Content ◽  
Relative Water ◽  
Leaf Relative Water Content

AbstractDrought can severely limit plant growth and production. However, few studies have investigated gene expression profiles in maize during drought/re-watering. We compared drought-treated and water-sufficient maize plants by measuring their leaf relative water content, superoxide dismutase and peroxidase activities, proline content, and leaf gas exchange parameters (photosynthetic rates, stomatal conductance, and transpiration rates). We conducted RNA sequencing analyses to elucidate gene expression profiles and identify miRNAs that might be related to drought resistance. A GO enrichment analysis showed that the common DEGs (differently expressed genes) between drought-treated and control plants were involved in response to stimulus, cellular process, metabolic process, cell part, and binding and catalytic activity. Analyses of gene expression profiles revealed that 26 of the DEGs under drought encoded 10 enzymes involved in proline synthesis, suggesting that increased proline synthesis was a key part of the drought response. We also investigated cell wall-related genes and transcription factors regulating abscisic acid-dependent and -independent pathways. The expression profiles of the miRNAs miR6214-3p, miR5072-3p, zma-miR529-5p, zma-miR167e-5p, zma-miR167f-5p, and zma-miR167j-5p and their relevant targets under drought conditions were analyzed. These results provide new insights into the molecular mechanisms of drought tolerance, and may identify new targets for breeding drought-tolerant maize lines.Abbreviationsleaf relative water content: RWC, superoxide dismutase activity: SOD, peroxidase activity: POD, proline content: Pro, photosynthetic rates: Pn, stomatal conductance: Cond, transpiration rates: Tr.; quantitative real-time polymerase chain reaction: qPCR; abscisic acid; ABA; polyethylene glycol :PEG; Principal component analysis :PCA; polyacrylamide gel electrophoresis :PAGEHighlightThe study of physiology and molecular mechanism of maize laid a theoretical foundation for drought resistance breeding under drought stress and re-watering.

scholarly journals Gene Expression Analysis in Cold Stress Conditions Reveals BBX20 and CLO as Potential Biomarkers for Cold Tolerance in Almond

Horticulturae ◽  
2021 ◽  
Vol 7 (12) ◽  
pp. 527
Author(s):  
Beatriz Bielsa ◽  
Jorge Israel Ávila-Alonso ◽  
Ángel Fernández i Martí ◽  
Jérôme Grimplet ◽  
María José Rubio-Cabetas
Keyword(s):  
Gene Expression ◽  
Cold Stress ◽  
Cold Tolerance ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Limiting Factors ◽  
Cold Response ◽  
Spring Frost ◽  
Phenotype Analysis ◽  
Spring Frosts

Late spring frosts can become one of the limiting factors for the expansion of cultivation area towards a harsher climate for the almond [Prunus amygdalus Batsch syn P. dulcis (Mill.) D.A. Webb] crop as spring frost can damage up to 90% of the harvest. In order to identify key genes favoring cold tolerance in almonds, branches from three late-blooming genotypes: ‘Guara’, ‘Soleta’ and ‘Belona’ were exposed at −4 °C during 24 h in a constant climate chamber. Phenotype analysis showed that ‘Guara’ and ‘Soleta’ had a greater acclimation capacity to cold than ‘Belona’. The qRT-PCR BioMark System technology was used to monitor the relative expression of 30 candidate genes with a potential relation to cold response, which are either involved in the ICE-CBF-COR pathway or the independent CBF pathway, and also genes not yet characterized or with unknown function in almond genome. Differences in the gene expression profiles were found among the three studied genotypes and the three time-points of cold exposure (0, 2 and 24 h). BBX20 and CLO genes behaved as differentiator genes between tolerant and susceptible genotypes in cold stress response in almond pistils. In addition, the differences of expression among the tolerant genotypes suggested the intervention of different mechanisms responding to cold stress in almonds.

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