scholarly journals Gene Expression Profiles Suggest a Better Cold Acclimation of Polyploids in the Alpine Species Ranunculus kuepferi (Ranunculaceae)

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1818
Author(s):  
Eleni Syngelaki ◽  
Claudia Paetzold ◽  
Elvira Hörandl
Keyword(s):  
Gene Expression ◽  
Cold Stress ◽  
Cold Acclimation ◽  
Temperature Stress ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Distribution Patterns ◽  
Gene Set ◽  
Cation Homeostasis ◽  
Response To Temperature

Alpine habitats are shaped by harsh abiotic conditions and cold climates. Temperature stress can affect phenotypic plasticity, reproduction, and epigenetic profiles, which may affect acclimation and adaptation. Distribution patterns suggest that polyploidy seems to be advantageous under cold conditions. Nevertheless, whether temperature stress can induce gene expression changes in different cytotypes, and how the response is initialized through gene set pathways and epigenetic control remain vague for non-model plants. The perennial alpine plant Ranunculus kuepferi was used to investigate the effect of cold stress on gene expression profiles. Diploid and autotetraploid individuals were exposed to cold and warm conditions in climate growth chambers and analyzed via transcriptome sequencing and qRT-PCR. Overall, cold stress changed gene expression profiles of both cytotypes and induced cold acclimation. Diploids changed more gene set pathways than tetraploids, and suppressed pathways involved in ion/cation homeostasis. Tetraploids mostly activated gene set pathways related to cell wall and plasma membrane. An epigenetic background for gene regulation in response to temperature conditions is indicated. Results suggest that perennial alpine plants can respond to temperature extremes via altered gene expression. Tetraploids are better acclimated to cold conditions, enabling them to colonize colder climatic areas in the Alps.

scholarly journals Conserved immunomodulatory transcriptional networks underlie antipsychotic-induced weight gain

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rizaldy C. Zapata ◽  
Besma S. Chaudry ◽  
Mariela Lopez Valencia ◽  
Dinghong Zhang ◽  
Scott A. Ochsner ◽  
...  
Keyword(s):  
Gene Expression ◽  
Weight Gain ◽  
Regulatory Networks ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Whole Body ◽  
Transcriptional Networks ◽  
Gene Set ◽  
P Gene ◽  
Whole Body Metabolism

AbstractAlthough antipsychotics, such as olanzapine, are effective in the management of psychiatric conditions, some patients experience excessive antipsychotic-induced weight gain (AIWG). To illuminate pathways underlying AIWG, we compared baseline blood gene expression profiles in two cohorts of mice that were either prone (AIWG-P) or resistant (AIWG-R) to weight gain in response to olanzapine treatment for two weeks. We found that transcripts elevated in AIWG-P mice relative to AIWG-R are enriched for high-confidence transcriptional targets of numerous inflammatory and immunomodulatory signaling nodes. Moreover, these nodes are themselves enriched for genes whose disruption in mice is associated with reduced body fat mass and slow postnatal weight gain. In addition, we identified gene expression profiles in common between our mouse AIWG-P gene set and an existing human AIWG-P gene set whose regulation by immunomodulatory transcription factors is highly conserved between species. Finally, we identified striking convergence between mouse AIWG-P transcriptional regulatory networks and those associated with body weight and body mass index in humans. We propose that immunomodulatory transcriptional networks drive AIWG, and that these networks have broader conserved roles in whole body-metabolism.

scholarly journals Gene Expression Analysis in Cold Stress Conditions Reveals BBX20 and CLO as Potential Biomarkers for Cold Tolerance in Almond

Horticulturae ◽  
2021 ◽  
Vol 7 (12) ◽  
pp. 527
Author(s):  
Beatriz Bielsa ◽  
Jorge Israel Ávila-Alonso ◽  
Ángel Fernández i Martí ◽  
Jérôme Grimplet ◽  
María José Rubio-Cabetas
Keyword(s):  
Gene Expression ◽  
Cold Stress ◽  
Cold Tolerance ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Limiting Factors ◽  
Cold Response ◽  
Spring Frost ◽  
Phenotype Analysis ◽  
Spring Frosts

Late spring frosts can become one of the limiting factors for the expansion of cultivation area towards a harsher climate for the almond [Prunus amygdalus Batsch syn P. dulcis (Mill.) D.A. Webb] crop as spring frost can damage up to 90% of the harvest. In order to identify key genes favoring cold tolerance in almonds, branches from three late-blooming genotypes: ‘Guara’, ‘Soleta’ and ‘Belona’ were exposed at −4 °C during 24 h in a constant climate chamber. Phenotype analysis showed that ‘Guara’ and ‘Soleta’ had a greater acclimation capacity to cold than ‘Belona’. The qRT-PCR BioMark System technology was used to monitor the relative expression of 30 candidate genes with a potential relation to cold response, which are either involved in the ICE-CBF-COR pathway or the independent CBF pathway, and also genes not yet characterized or with unknown function in almond genome. Differences in the gene expression profiles were found among the three studied genotypes and the three time-points of cold exposure (0, 2 and 24 h). BBX20 and CLO genes behaved as differentiator genes between tolerant and susceptible genotypes in cold stress response in almond pistils. In addition, the differences of expression among the tolerant genotypes suggested the intervention of different mechanisms responding to cold stress in almonds.

scholarly journals A Regulatory Network of Heat Shock Modules-Photosynthesis-Redox Systems in Response to Cold Stress Across a Latitudinal Gradient in Bermudagrass

2021 ◽  
Vol 12 ◽  
Author(s):  
Minghui Chen ◽  
Lu Gan ◽  
Jingxue Zhang ◽  
Yu Shen ◽  
Jin Qian ◽  
...  
Keyword(s):  
Cold Stress ◽  
Temperature Stress ◽  
Transcriptome Analysis ◽  
Cynodon Dactylon ◽  
Gene Annotation ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Redox System ◽  
Latitudinal Gradients ◽  
Redox Systems

Bermudagrass (Cynodon dactylon Pers.) is a wild Poaceae turfgrass with various genotypes and phenotypes. In this study, 16 wild bermudagrass germplasms were collected from 16 different sites along latitudinal gradients, and different temperature treatments were compiled and used for physiological and transcriptome analysis. To explore the correlation between the key differentially expressed genes and physiological indicators, a total of 14,654 DEGs were integrated from the comparison of different temperature treatments and used for weighted gene co-expression network analysis. Through comparative transcriptome analysis and gene annotation, the results showed that differential gene expression profiles in networks are associated with the plant growth, photosystem, redox system, and transcriptional regulation to cold stress in bermudagrass. In particular, genes encoding HSP70/90 and HsfA3/A8 are not only regulated by temperature stress, but also directly or indirectly interplay with the processes of peroxide scavenging and chlorophyll synthesis under cold stress. Besides, through a weight evaluation analysis of various physiological indexes, we identified an accession of wild bermudagrass with relatively strong cold resistance. These results provide important clues and resources to further study the responses to low-temperature stress in bermudagrass.

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