Established UPLC-MS/MS procedure for multicomponent quantitative analysis in rat plasma: A contrastive pharmacokinetics study of Qiangshen tablet in normal and kidney yang deficiency syndrome models

2019 ◽  
Vol 1106-1107 ◽  
pp. 35-42 ◽  
Author(s):  
Jinwei Gao ◽  
Jinbo Yu ◽  
Panpan Xu ◽  
Jinmei Chen ◽  
Guanghui Gao ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Deficiency Syndrome ◽  
Rat Plasma ◽  
Pharmacokinetics Study ◽  
Kidney Yang Deficiency

scholarly journals The Difference of Chemical Components and Biological Activities of the Crude Products and the Salt-Processed Product from Semen Cuscutae

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Song Yang ◽  
Hefang Xu ◽  
Baosheng Zhao ◽  
Shasha Li ◽  
Tingting Li ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Biological Activities ◽  
Biological Effects ◽  
Principal Component ◽  
Deficiency Syndrome ◽  
Antioxidant Effect ◽  
Chemical Components ◽  
Crude Product ◽  
Kidney Yang Deficiency ◽  
The Difference

Semen Cuscutae is a well-known Chinese medicine which has been used to nourish kidney in China for thousands of years. The crude product of semen Cuscutae (CP) and its salt-processed product (SPP) are separately used in clinic for their different effects. The study was designed to investigate the influence of processing from semen Cuscutae on chemical components and biological effects. The principal component analysis and quantitative analysis were used to study the differences of the chemical components. The effects of nourishing kidney were detected to compare the differences between the CP and SPP. The PCA results showed that the obvious separation was achieved in the CP and SPP samples. The results of quantitative analysis showed that quercetin and total flavonoids had significantly increased after salt processing while hyperoside had decreased. The comparison of CP and SPP on biological activities showed that both of them could ameliorate the kidney-yang deficiency syndrome by restoring the level of sex hormone, improving the immune function and antioxidant effect. However, SPP was better in increasing the level of T and the viscera weight of testicle and epididymis, improving the antioxidant effect. The results suggested that salt processing changed its chemical profile, which in turn enhanced its biological activities.

Quantitative Analysis and Pharmacokinetics Study of Integrin Antagonist AP25 in Rat Plasma

2013 ◽  
Vol 20 (5) ◽  
pp. 584-590
Author(s):  
Yongbing Li ◽  
Hanmei Xu ◽  
Jialiang Hu ◽  
Fengguo Zhang ◽  
Zhendong Liu ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Rat Plasma ◽  
Pharmacokinetics Study

A profluorescent nitroxide probe for ascorbic acid detection and its application to quantitative analysis of diabetic rat plasma

RSC Advances ◽  
2016 ◽  
Vol 6 (65) ◽  
pp. 60907-60915 ◽  
Author(s):  
Yuta Matsuoka ◽  
Kei Ohkubo ◽  
Toshihide Yamasaki ◽  
Mayumi Yamato ◽  
Hiroshi Ohtabu ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Quantitative Analysis ◽  
Rat Plasma ◽  
Diabetic Rat ◽  
Nitroxide Probe ◽  
Ascorbic Acid Detection

15-((9-(Ethylimino)-10-methyl-9Hbenzo[a]phenoxazin-5-yl)amino)-3,11-dioxa-7-azadispiro[5.1.58.36]hexadecan-7-yloxyl, (Nile-DiPy) has been synthesized and examined as an off–on profluorescent nitroxide probe for measuring ascorbic acid in plasma.

A rapid HPLC/ESI-MS/MS method for quantitative analysis of isovalerylshikonin in rat plasma

2009 ◽  
pp. n/a-n/a
Author(s):  
MeiXiang Zhu ◽  
Yanbing Gao ◽  
Zhen Wu ◽  
YuFen Zhao
Keyword(s):  
Quantitative Analysis ◽  
Rat Plasma ◽  
Esi Ms

scholarly journals Effects of Haima Duobian Pill in a Rat Model of Kidney Yang Deficiency Syndrome

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Yijia Zeng ◽  
Tingna Li ◽  
Xiaorui Zhang ◽  
Yuanyuan Ren ◽  
Qinwan Huang ◽  
...  
Keyword(s):  
Rat Model ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Cyclic Guanosine Monophosphate ◽  
Deficiency Syndrome ◽  
Guanosine Monophosphate ◽  
Network Pharmacology ◽  
Enzyme Linked Immunosorbent Assay ◽  
Therapeutic Effects ◽  
Kidney Yang Deficiency

Objective. Modern research shows that Haima Duobian pill (HDP) can relieve the kidney yang deficiency syndrome (KYDS), but the mechanism is still unclear. The aim of this work was to study the effects of HDP in a rat model of KYDS. Materials and Methods. The network pharmacology methods were used to predict the therapeutic effects of Haima Duobian pill. Adenine was used to establish the rat model of kidney yang deficiency syndrome. The general physical signs of rats were observed after different doses of Haima Duobian pill (HDP) were given. Serum cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP), luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone (T), estradiol (E2), and gonadotropin-releasing hormone (GnRH) levels were determined using enzyme-linked immunosorbent assay (ELISA) kits. Then, the histopathologic changes and sperm activity were detected. Results. HDP could improve the general signs of kidney yang deficiency syndrome rats. After the rats were treated with HDP, the expression of cGMP and E2 was significantly inhibited and the expression of cAMP and T was significantly increased. The pathological damage of testis, epididymis, and seminal vesicle was alleviated, and the sperm activity was improved. Conclusion. For adenine-induced kidney yang deficiency syndrome in rats, HDP had a significant therapeutic effect.

Simultaneous determination of chito-oligosaccharide in rat plasma by LC-MS/MS method: Application to a pharmacokinetics study

2021 ◽  
Author(s):  
Pengpeng Zhang ◽  
Liu Shuai ◽  
Shuang Yang ◽  
Yuanhong Wang ◽  
Ting-Fu Jiang ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Rat Plasma ◽  
Plasma Samples ◽  
Pharmacokinetics Study ◽  
Sensitive Method

A simple and sensitive method for the simultaneous determination of chito-oligosaccharide (COS) with degrees of polymerization（DPs）from 2 to 7 was developed and used for COS quantification in rat plasma. Samples...

scholarly journals The Difference of Chemical Components and Biological Activities of the Raw Products slices and the Wine Steam-Processed Product from Cistanche deserticola

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Ying Zhang ◽  
Yuewu Wang ◽  
Song Yang ◽  
Yunfeng Xiao ◽  
Haibin Guan ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Biological Activities ◽  
Biological Effects ◽  
Chinese Herb ◽  
Principal Component ◽  
Deficiency Syndrome ◽  
Chemical Components ◽  
Chemical Compositions ◽  
Kidney Deficiency ◽  
Cistanche Deserticola

As a well-known Chinese herb medicine, the Cistanche deserticola has been used for the treatment of kidney deficiency syndrome in China for thousands of years. Both the raw product of Cistanche deserticola slices (RCD) and its Wine Steam-Processed Product (WSCD) are used clinically for different effects. In this study, the influences of steaming process with wine (SPW) from Cistanche deserticola on chemical compositions and biological effects were investigated. The principal component analysis (PCA) and quantitative analysis were used to study the differences of the chemical compositions. The effects of nourishing kidney were also investigated to compare the differences between the RCD and the WSCD. The PCA results indicated that the obvious separation was achieved in the RCD and WSCD. The results of quantitative analysis showed that the WSCD has higher amounts of total polysaccharides, total PhGs, isoacteoside, and osmanthuside B than RCD, while the content of 2′-acetylacteoside and acteoside decreased after the SPW. The comparison of RCD and WSCD on biological activities showed that both could restore the level of sex hormone in the model of kidney-yang deficiency and improve the antioxidant effect. The WSCD were much better in increasing the viscera weight of kidney and seminal vesicle. The results indicated that SPW changed its chemical components and enhanced its biological activities.

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