Metabolomics and molecular docking to compare of the efficacies of wild-simulated and transplanted Astragali Radix based on its compatibility with Angelicae Sinensis Radix against blood deficiency

2021 ◽  
pp. 122682
Author(s):  
YueTao Liu ◽  
ZiYing Wang ◽  
XueMei Qin
Keyword(s):  
Molecular Docking ◽  
Astragali Radix ◽  
Angelicae Sinensis

scholarly journals Functions of Danggui Buxue Tang, a Chinese Herbal Decoction Containing Astragali Radix and Angelicae Sinensis Radix, in Uterus and Liver are Both Estrogen Receptor-Dependent and -Independent

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Oliver Zierau ◽  
Ken Y. Z. Zheng ◽  
Anja Papke ◽  
Tina T. X. Dong ◽  
Karl W. K. Tsim ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Ovariectomized Rats ◽  
Transcript Expression ◽  
Astragali Radix ◽  
Danggui Buxue Tang ◽  
Chinese Herbal ◽  
Herbal Decoction ◽  
Estrogenic Properties ◽  
Angelicae Sinensis

Danggui Buxue Tang (DBT), a herbal decoction containing Astragali Radix (AR) and Angelicae Sinensis Radix (ASR), has been used in treating menopausal irregularity in women for more than 800 years in China. Pharmacological results showed that DBT exhibited significant estrogenic propertiesin vitro, which therefore suggested that DBT could activate the nuclear estrogen receptors. Here, we assessed the estrogenic properties of DBT in an ovariectomizedin vivorat model: DBT was applied to the ovariectomized rats for 3 days. The application of DBT did not alter the weight of uterus and liver, as well as the transcript expression of the proliferation markers including the estrogen receptorsαandβ. However, DBT stimulated the transcript expression of the estrogen responsive genes. In addition, the inductive role of DBT on the expression of members of the aryl hydrocarbon receptor family in uterus and liver of ovariectomized rats was confirmed. These responses of DBT however were clearly distinct from the response pattern detectable here for 17β-estradiol. Therefore, DBT exhibited weak, but significant, estrogenic propertiesin vivo; however, some of its activities were independent of the estrogen receptor. Thus, DBT could be an exciting Chinese herbal decoction for an alternative treatment of hormone replacement therapy for women in menopause without subsequent estrogenic side effects.

scholarly journals Revealing the Mechanism of Astragali Radix against Cancer-Related Fatigue by Network Pharmacology and Molecular Docking

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Yi Xie ◽  
Kainan Zhou ◽  
Yan Wang ◽  
Shuhan Yang ◽  
Suying Liu ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Network Pharmacology ◽  
Active Compounds ◽  
Astragali Radix ◽  
Protein Protein Interaction ◽  
Topological Parameters ◽  
Kegg Analysis ◽  
The Core ◽  
Cancer Related Fatigue ◽  
Long Time

Background. Cancer-related fatigue (CRF) is an increasingly appreciated complication in cancer patients, which severely impairs their quality of life for a long time. Astragali Radix (AR) is a safe and effective treatment to improve CRF, but the related mechanistic studies are still limited. Objective. To systematically analyze the mechanism of AR against CRF by network pharmacology. Methods. TCMSP was searched to obtain the active compounds and targets of AR. The active compound-target (AC-T) network was established and exhibited by related visualization software. The GeneCards database was searched to acquire CRF targets, and the intersection targets with AR targets were used to make the Venny diagram. The protein-protein interaction (PPI) network of intersection targets was established, and further, the therapeutic core targets were selected by topological parameters. The selected core targets were uploaded to Metascape for GO and KEGG analysis. Finally, AutoDock Vina and PyMOL were employed for molecular docking validation. Results. 16 active compounds of AR were obtained, such as quercetin, kaempferol, 7-O-methylisomucronulatol, formononetin, and isorhamnetin. 57 core targets were screened, such as AKT1, TP53, VEGFA, IL-6, and CASP3. KEGG analysis manifested that the core targets acted on various pathways, including 137 pathways such as TNF, IL-17, and the AGE-RAGE signaling pathway. Molecular docking demonstrated that active compounds docked well with the core targets. Conclusion. The mechanism of AR in treating CRF involves multiple targets and multiple pathways. The present study laid a theoretical foundation for the subsequent research and clinical application of AR and its extracts against CRF.

scholarly journals The Osteogenic Function of Danggui Buxue Tang, a Herbal Decoction Containing Astragali Radix and Angelicae Sinensis Radix, Is Optimized by Boiling the Two Herbs Together: Signaling Analyses Revealed by Systems Biology

Processes ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 1119
Author(s):  
Kenneth K L Kwan ◽  
Tin Yan Wong ◽  
Anna X. D. Yu ◽  
Tina T. X. Dong ◽  
Henry H. L. Lam ◽  
...  
Keyword(s):  
Systems Biology ◽  
Herbal Extract ◽  
Complex Biological System ◽  
Astragali Radix ◽  
Danggui Buxue Tang ◽  
Herbal Mixture ◽  
Herbal Decoction ◽  
Cellular Pathways ◽  
Angelicae Sinensis ◽  
Rat Osteoblasts

The therapeutic efficacy of a herbal mixture, being multi-target, multi-function and multi-pathway, is the niche of traditional Chinese medicine (TCM). Systems biology can dissect the network of signaling mechanisms in a complex biological system. In preparing TCM decoctions, the boiling of herbs together in water is a common practice; however, the rationale of this specific preparation has not been fully revealed. An approach of mass-spectrometry-based multi-omics was employed to examine the profiles of the cellular pathways, so as to understand the pharmacological efficacy of Danggui Buxue Tang (DBT), a Chinese herbal mixture containing Astragali Radix and Angelicae Sinensis Radix, in cultured rat osteoblasts and mesenchymal stem cells. The results, generated from omics analyses, were compared from DBT-treated osteoblasts to those of treating the herbal extract by simple mixing of extracts from Astragali Radix and Angelicae Sinensis Radix, i.e., herbal mixture without boiling together. The signaling pathways responsible for energy metabolism and amino acid metabolism showed distinct activation, as triggered by DBT, in contrast to simple mixing of two herbal extracts. The result supports that boiling the herbs together is designed to maximize the osteoblastic function of DBT, such as in energy and lipid metabolism. This harmony of TCM formulation, by having interactive functions of two herbs during preparation, is being illustrated. The systems biology approach provides new and essential insights into the synergy of herbal preparation. Well-defined multiple targets and multiple pathways in different levels of omics are the key to modernizing TCM.

Altered Expression of Genes Profiles Modulated by a Combination of Astragali Radix and Angelicae Sinensis Radix in Obstructed Rat Kidney

Planta Medica ◽  
2010 ◽  
Vol 76 (13) ◽  
pp. 1431-1438 ◽  
Author(s):  
Liqiang Meng ◽  
Vicki Van Putten ◽  
Lei Qu ◽  
Raphael Nemenoff ◽  
Ming-Ying Shang ◽  
...  
Keyword(s):  
Rat Kidney ◽  
Altered Expression ◽  
Astragali Radix ◽  
Expression Of Genes ◽  
Angelicae Sinensis

scholarly journals Anticonstriction Effect of MCA in Rats by Danggui Buxue Decoction

2021 ◽  
Vol 12 ◽  
Author(s):  
Ying Guo ◽  
Yating Zhang ◽  
Ya Hou ◽  
Pengmei Guo ◽  
Xiaobo Wang ◽  
...  
Keyword(s):  
Channel Blockers ◽  
Vasodilator Effect ◽  
Astragali Radix ◽  
Synergistic Interactions ◽  
Low Concentrations ◽  
Channel Opening ◽  
High Concentrations ◽  
Identical Series ◽  
Danggui Buxue Decoction ◽  
Angelicae Sinensis

Objective: Danggui Buxue decoction (DBD), consisting of Angelicae Sinensis Radix (ASR) and Astragali Radix (AR), is a famous prescription with the function of antivasoconstriction. This study intends to probe its mechanisms on the relaxation of the middle cerebral artery (MCA).Methods: Vascular tension of rat MCA was measured using a DMT620 M system. First, the identical series of concentrations of DBD, ASR, and AR were added into resting KCl and U46619 preconstricted MCA. According to the compatibility ratio, their dilatation effects were further investigated on KCl and U46619 preconstricted vessels. Third, four K+ channel blockers were employed to probe the vasodilator mechanism on KCl-contracted MCA. We finally examined the effects of DBD, ASR, and AR on the vascular tone of U46619-contracted MCA in the presence or absence of Ca2+.Results: Data suggested that DBD, ASR, and AR can relax on KCl and U46619 precontracted MCA with no effects on resting vessels. The vasodilator effect of ASR was greater than those of DBD and AR on KCl-contracted MCA. For U46619-contracted MCA, ASR showed a stronger vasodilator effect than DBD and AR at low concentrations, but DBD was stronger than ASR at high concentrations. Amazingly, the vasodilator effect of DBD was stronger than that of AR at all concentrations on two vasoconstrictors which evoked MCA. The vasodilator effect of ASR was superior to that of DBD at a compatibility ratio on KCl-contracted MCA at low concentrations, while being inferior to DBD at high concentrations. However, DBD exceeded AR in vasodilating MCA at all concentrations. For U46619-constricted MCA, DBD, ASR, and AR had almost identical vasodilation. The dilation of DBD and AR on KCl-contracted MCA was independent of K+ channel blockers. However, ASR may inhibit the K+ channel opening partially through synergistic interactions with Gli and BaCl2. DBD, ASR, and AR may be responsible for inhibiting [Ca2+]out, while ASR and AR can also inhibit [Ca2+]in.Conclusion: DBD can relax MCA with no effects on resting vessels. The mechanism may be related to ASR’s inhibition of KATP and Kir channels. Meanwhile, the inhibition of [Ca2+]out by DBD, ASR, and AR as well as the inhibition of [Ca2+]in by ASR and AR may contribute to dilate MCA.

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