In this study, a simple, sensitive and reliable fingerprint analysis method by high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) was developed for quality analysis of Bulbus Lilii, which is a famous traditional Chinese medicine and widely used as a restorative food. Then the developed method was subsequently applied to analyse 16 samples collected or purchased from different origins. Twelve common fingerprint peaks, including four definitely identified peaks, were found in total, and were involved for further characterisation and quality evaluation of Bulbus Lilii. The chemical quality of commercial samples collected from different origins varied obviously, indicating that more attention should be paid to the standardisation of the process of harvesting, manufacturing and storage of Bulbus Lilii. This is the first time that a new method has been established to control the quality of Bulbus Lilii through HPLC-DAD.