Nano- and micro-mechanical properties of wheat grain by atomic force microscopy (AFM) and nano-indentation (IIT) and their relationship with the mechanical properties evaluated by uniaxial compression test

2019 ◽  
Vol 90 ◽  
pp. 102830 ◽  
Author(s):  
Gabriela Noel Barrera ◽  
Juan Méndez-Méndez ◽  
Israel Arzate-Vázquez ◽  
Georgina Calderón-Domínguez ◽  
Pablo Daniel Ribotta
2021 ◽  
Author(s):  
Wenyan Yu ◽  
Tong Liu ◽  
Jianqing Mi ◽  
hyock ju Kwon

Abstract Background Cell mechanics focuses on the mechanical properties of the cells and how they affect biological behaviors of cells. Mechanical property change of cells can reflect specific diseases and conditions. Therefore, detecting the changes in cell mechanical properties can potentially be associated with diagnosis and treatment of diseases.MethodsMechanical properties of living and dead human corneal epithelial cells (HCECs) were investigated with nano-indentation method using atomic force microscopy (AFM). Sneddon model was adopted to analyze the contacts between pyramidal AFM tip and soft materials. Polyacrylamide (PAAm) substrates with different stiffness were fabricated and human corneal epithelial cells (HCECs) were cultured on them. By applying nano-indentation to HCECs, we examined the distribution of elasticity across cells, and studied the differences between living and dead cells. Findings The central part of a cell, closer to nucleus, was more compliant than the outer regions, toward the surface of the cell’s lipid bilayer. Also, HCECs became stiffer as the stiffness of the substrate increased. Moreover, it was found that dead cells have much higher elastic moduli and exhibited elastic behavior, while living cells showed highly viscoelastic behavior.InterpretationAFM probing is an effective tool for characterizing cell mechanical behavior. More indentation results should be made in further studies to form statistical model of elasticity distribution for HCECs. This study can be extended to investigate the mechanical behaviors of benign and malignant cells or drug interactions on cells, which can establish more probable mechanical property–disease connections.


2000 ◽  
Vol 39 (Part 1, No. 6B) ◽  
pp. 3711-3716 ◽  
Author(s):  
Hatsuki Shiga ◽  
Yukako Yamane ◽  
Etsuro Ito ◽  
Kazuhiro Abe ◽  
Kazushige Kawabata ◽  
...  

Coatings ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 652
Author(s):  
Divine Sebastian ◽  
Chun-Wei Yao ◽  
Lutfun Nipa ◽  
Ian Lian ◽  
Gary Twu

In this work, a mechanically durable anticorrosion superhydrophobic coating is developed using a nanocomposite coating solution composed of silica nanoparticles and epoxy resin. The nanocomposite coating developed was tested for its superhydrophobic behavior using goniometry; surface morphology using scanning electron microscopy and atomic force microscopy; elemental composition using energy dispersive X-ray spectroscopy; corrosion resistance using atomic force microscopy; and potentiodynamic polarization measurements. The nanocomposite coating possesses hierarchical micro/nanostructures, according to the scanning electron microscopy images, and the presence of such structures was further confirmed by the atomic force microscopy images. The developed nanocomposite coating was found to be highly superhydrophobic as well as corrosion resistant, according to the results from static contact angle measurement and potentiodynamic polarization measurement, respectively. The abrasion resistance and mechanical durability of the nanocomposite coating were studied by abrasion tests, and the mechanical properties such as reduced modulus and Berkovich hardness were evaluated with the aid of nanoindentation tests.


Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1661
Author(s):  
Katarzyna Adamiak ◽  
Katarzyna Lewandowska ◽  
Alina Sionkowska

Collagen films are widely used as adhesives in medicine and cosmetology. However, its properties require modification. In this work, the influence of salicin on the properties of collagen solution and films was studied. Collagen was extracted from silver carp skin. The rheological properties of collagen solutions with and without salicin were characterized by steady shear tests. Thin collagen films were prepared by solvent evaporation. The structure of films was researched using infrared spectroscopy. The surface properties of films were investigated using Atomic Force Microscopy (AFM). Mechanical properties were measured as well. It was found that the addition of salicin modified the roughness of collagen films and their mechanical and rheological properties. The above-mentioned parameters are very important in potential applications of collagen films containing salicin.


2018 ◽  
Vol 114 (3) ◽  
pp. 513a
Author(s):  
Yuri M. Efremov ◽  
Mirian Velay-Lizancos ◽  
Daniel M. Suter ◽  
Pablo D. Zavattieri ◽  
Arvind Raman

PLoS ONE ◽  
2012 ◽  
Vol 7 (1) ◽  
pp. e30204 ◽  
Author(s):  
David Martinez-Martin ◽  
Carolina Carrasco ◽  
Mercedes Hernando-Perez ◽  
Pedro J. de Pablo ◽  
Julio Gomez-Herrero ◽  
...  

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