Green fabrication, characterization of silver nanoparticles using aqueous leaf extract of Ocimum americanum (Hoary Basil) and investigation of its in vitro antibacterial, antioxidant, anticancer and photocatalytic reduction

2021 ◽  
Vol 9 (1) ◽  
pp. 104845
Author(s):  
Dinesh Babu Manikandan ◽  
Arun Sridhar ◽  
Rajkumar Krishnasamy Sekar ◽  
Balaji Perumalsamy ◽  
Srinivasan Veeran ◽  
...  
Author(s):  
Inbathamizh L ◽  
Kalpana V ◽  
Revathi Yadav K

With the increase in the potential applications of nanoparticles in pharma and various fields, nanoparticle research is attracting more attention. Though several chemical and physical methods are being used for the synthesis of metal nanoparticles, they are associated with several disadvantages. Couroupita guianensis is a traditional plant with medicinal values. The focus of the study was to follow a green chemistry route to synthesize silver nanoparticles (AgNPs) using the leaf extract of Couroupita guianensis as a reductant and stabilizing agent. The boiled aqueous leaf extract with silver nitrate solution on exposure to sunlight showed the maximum absorbance at 430nm indicating the synthesis of AgNPs. Ultra Violet (UV)-Visible spectroscopy, Scanning Electron Microscopy (SEM), and Fourier Transform Infrared spectroscopy (FTIR) techniques were used for the characterization of AgNPs. The synthesized AgNPs were found to be spherical and 4.44 – 40.20nm in size. They also seemed to be capped with the significant functional groups present in the leaf extract. Thus, the study suggested Couroupita guianensis mediated green synthesis of AgNPs as an efficient and eco-friendly approach with substantial advantages over the conventional methods. The process could be further scaled-up for mass production and wider applications of AgNPs.


Author(s):  
I. O. Salaudeen ◽  
M. O. Olajuwon ◽  
A. B. Ajala ◽  
T. O. Abdulkareem ◽  
S. A. Adeniyi ◽  
...  

This study investigated the synthesis, characterization and in vitro antioxidant activity of silver nanoparticles (AgNPs) using the aqueous leaf extract of Justicia carnea.  The aqueous leaf extract of J. carnea was used as a potential reducing and capping agent. To identify the compounds responsible for the reduction of silver ions, the functional groups present in the plant extract were subjected to FTIR. The in vitro antioxidant activity of synthesized nanoparticles was evaluated in terms of ferric reducing antioxidant potential (FRAP), DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2`-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) free radicals scavenging assays. The surface plasmon resonance confirmed the formation of AgNPs with maximum absorbance at kmax = 446 nm. FTIR revealed the biological macromolecules of J. carnea leaf extract involved in the synthesis and stabilization of AgNPs. UV-Visible spectrophotometer showed absorbance peak in the range of 436-446 nm. The silver nanoparticles exhibited moderate antioxidant activities compared to standard antioxidants (ascorbic acid and BHT). These results confirmed this protocol as simple, eco-friendly, nontoxic and an alternative for conventional physical and chemical methods. It can be concluded that J. carnea leaf extract can be used effectively in the production of potential antioxidant AgNPs which could be useful in various bio-applications such as cosmetics, food and biomedical industry.


2015 ◽  
Vol 6 (7) ◽  
pp. 973-982 ◽  
Author(s):  
Gunaseelan Kuppurangan ◽  
Balaji Karuppasamy ◽  
Kanipandian Nagarajan ◽  
Rajkumar Krishnasamy Sekar ◽  
Nilmini Viswaprakash ◽  
...  

Author(s):  
S. Balasubramanian ◽  
U. Jeyapaul ◽  
S. Mary Jelastin Kala

The present investigation, we describe a cost effective biofabrication of silver nanoparticles using jasminum auriculatum leaf extract. The aqueous leaf extract of jasminum auriculatum are used as reducing agent and capping agent in silver nanoparticles synthesis. The biofabricated silver nanoparticles were characterized by UV-Visible spectrophotometer, X-Ray Diffraction, FTIR spectra and SEM with EDX analysis. FTIR studies revealed that the functional group responsible for silver reduction were found to be jasminol and triterpinoid compounds present in leaf extract. XRD analysis showed that prepared silver nanoparticles are crystalline in nature with an average diameter of 15-20 nm. The prepared silver nanoparticles have been evaluated in vitro for antibacterial activities and found to have higher antibacterial activities.


2017 ◽  
Vol 7 (3) ◽  
pp. 439-449 ◽  
Author(s):  
Vinodhini Velu ◽  
Moonjit Das ◽  
Arunai Nambi Raj N ◽  
Kamal Dua ◽  
Himaja Malipeddi

Author(s):  
M. Linga Rao ◽  
Bhumi G ◽  
Savithramma N

Silver nanoparticles (SNPs) exhibit tremendous applications in medicine as antimicrobial agent.  The use of different parts of plants for the synthesis of nanoparticles is considered as a green technology as it does not involve any harmful chemicals.  In the present study, we report a rapid biosynthesis of silver nanoparticles from aqueous leaf extract of medicinal plant Allamanda cathartica.  The active phytochemicals present in the plant were responsible for the quick reduction of silver ion to metallic silver nanoparticles. The reduced silver nanoparticles were characterized by using UV-Vis spectrophotometry, Scanning Electron Microscope (SEM), Energy Dispersive Analysis of X-ray (EDAX) and Atomic Force Microscopy (AFM).  The spherical shaped silver nanoparticles were observed and it was found to 19-40 nm range of size.  These phytosynthesized SNPs were tested for their antimicrobial activity and it analyzed by measuring the inhibitory zone. A. cathartica aqueous leaf extract of SNPs showed highest toxicity to Pseudomonas followed by Klebsiella, Bacillus and E. coli and lowest toxicity towards Proteus. In fungal species, highest inhibition zone was noted against Rhizopus followed by Curvularia, Aspergillus flavus and Aspergillus niger and minimum inhibition zone was observed against Fusarium species.  These results suggest a promising potential of Indian plant-based green chemistry for production of SNPs for biomedical and nanotechnology applications.


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