Dose- and time-dependent effects of ethanolic extract of Mucuna pruriens Linn. seed on sexual behaviour of normal male rats

Male rats were treated daily with oil or 100 μg of the antioestrogen, ethamoxytriphetol (MER-25), for the first 10 days of life and, when adult, lesions were made in the suprachiasmatic nuclei (SCN) of the hypothalamus or control lesions were made above the SCN and the rats were tested for sexual behaviour. Treatment with MER-25 enhanced the daily rhythmicity in both mounting and lordosis behaviour and SCN lesions disrupted these behavioural rhythms and the rhythm in the mounting behaviour of oil-treated rats. Rats treated with MER-25 and with SCN lesions showed high levels of mounting and lordosis behaviour throughout the light: darkness cycle. These results support the hypothesis that sexual differentiation by perinatal androgen stimulation uncouples the central rhythm generator from the neural substrates of sexual behaviour in rats.

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Age dynamics of changes in sexual behavior and androgen status of male rats caused by prenatal exposure to low dose dibutylphthalate

JOURNAL OF THE NATIONAL ACADEMY OF MEDICAL SCIENCES OF UKRAINE ◽

10.37621/jnamsu-2019-3-260-267 ◽

2019 ◽

pp. 260-267

Author(s):

Alexander Reznikov ◽

Olha Sachynska ◽

Аnna Lymareva

Keyword(s):

Sexual Behavior ◽

Sexual Behaviour ◽

Low Dose ◽

Statistical Significance ◽

Fold Increase ◽

Premature Aging ◽

Male Rats ◽

Plasma Testosterone ◽

Normal Male ◽

Male Sexual Behavior

The aim of this work was to study the sexual maturation, testicular hormonal activity and sexual behavior of Wistar male rats, which were born to mothers treated intragastrically with di-n-butylphtha- late (DBP) for 15-21 days of their pregnancy, at a daily dose of 100 mg/kg b.w., that do not cause visible developmental morpho- logical abnormalities. Their sexual behaviour was analyzed via the proceptive reactions, the latent and refractory periods of time and number of mounts, intromissions and ejaculations at the presence of a receptive female. Female sexual behaviour was evaluated by lordosis responses of orchidectomized and activated with estradiol and progesterone males in the presence of a normal male. Prenatal administration of DBP induced an accelerated onset of puberty. In young mature males (6-month old), it was, unexpectedly (as for endocrine disruptors), discovered that was featured by hyperactive central (motivational) and peripheral (copulative and ejaculatory) components of male sexual behavior and a two-fold increase in the average levels of plasma testosterone. In 10-month old rats, we found both homosexual and enhanced lordotic behavior. In aging animals (18 months), hyperandrogenism had switched to quite the opposite condition: more than two-fold decrease in the levels of plasma testosterone, close to statistical significance; and a lessen- ing of male sexual behavior compared to controls. These findings show the presence of epigenetic alterations in the programming of the parts of the brain responsible for sexual differentiation (the hypermasculinization combined with feminization) and a regula- tion of testicular hormonopoeisis, as well as premature aging of the reproductive organs resulting from the prenatal influence of low dose DBP. Thereby we proposed the original concept of prenatally determined DBP syndrome. And, finally, the possible mechanism of these changes in the repro- ductive system are discussed. Key words: dibutylphthalate; prenatal effect; sexual behavior; tes- tosterone; male rats. For citation: Reznikov AG, Sachynska OV, Lymareva АA.

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Pueraria tuberosaDC Extract Improves Androgenesis and Sexual Behavior via FSH LH Cascade

The Scientific World JOURNAL ◽

10.1155/2013/780659 ◽

2013 ◽

Vol 2013 ◽

pp. 1-8 ◽

Cited By ~ 10

Author(s):

Nagendra Singh Chauhan ◽

Vikas Sharma ◽

Mayank Thakur ◽

Alexandra Christine Helena Frankland Sawaya ◽

V. K. Dixit

Keyword(s):

Sexual Behavior ◽

Sexual Behaviour ◽

Ethanolic Extract ◽

Treated Group ◽

Male Rats ◽

Biochanin A ◽

Control Group ◽

Albino Rats ◽

Testis Weight ◽

Female Rat

The aim of this study was to investigate the effects of ethanolic extract ofPueraria tuberosa(PT) on sexual behaviour and androgenic activity. Male albino rats were divided into four groups of six animals each: control group 1 (2% acacia solution), PT-treated group 2 (50 mg/Kg), PT-treated group 3 (100 mg/Kg), and PT-treated group 4 (150 mg/Kg). Sexual behavior of male rats in the presence of a female rat was recorded. The treated groups were evaluated for sexual parameters. The extract was characterized using LC-MS. The effect of treatment on anabolic and weight of secondary sexual organs was determined. The histological changes in section of testis and epididymis after treatment were observed. Sperm count in epididymis and fructose content in seminal vesicles were also measured. Levels of hormones like FSH, LH, and T were determined. A dose-dependent increase in sexual behaviors was evidenced in the animals of extract treated groups. Increase in testis weight was recorded in PT. At the highest dose PT also affects the hormones level. The four compounds namely puerarin, daidzein, biochanin-A and formononetin were identified in ethanolic extract using LC-MS. It concluded that PT extract possesses androgenic effect and it significantly increased the sexual behaviour and hormones level.

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LORDOSIS BEHAVIOUR IN MALE RATS: EFFECT OF DEAFFERENTATION IN THE PREOPTIC AREA AND HYPOTHALAMUS

Journal of Endocrinology ◽

10.1677/joe.0.0760381 ◽

1978 ◽

Vol 76 (2) ◽

pp. 381-382 ◽

Cited By ~ 22

Author(s):

K. YAMANOUCHI ◽

Y. ARAI

Keyword(s):

Sexual Behaviour ◽

Preoptic Area ◽

Male Rats ◽

Normal Male ◽

Afferent Pathway ◽

School Of Medicine ◽

Neonatal Life ◽

Lordosis Behaviour

Department of Anatomy, Juntendo University School of Medicine, Hongo, Tokyo 113, Japan (Received 18 October 1977) Differentiation of sexual behaviour patterns in male rats is dependent on the internal secretion of the testes during neonatal life. Removal of the testes at this time causes feminization and results in male rats which display female patterns of sexual behaviour (Gorski, 1971). Female patterns of behaviour are usually rare in normal male rats but recently we found that transection of the dorsal afferent neurones to the preoptic and anterior hypothalamic areas potentiated the display of lordosis in hormonally primed male rats (Yamanouchi & Arai, 1975). In the present study, further neuroanatomical analysis was carried out to clarify the localization of the afferent pathway involved in the regulation of lordosis behaviour. Anterior or posterior roof deafferentation (ARD or PRD) was performed by lowering an L-shaped Halasz knife (2-5 mm horizontal blade) to the level

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DER EINFLUSS VON RESERPIN AUF DIE ANTITHYREOIDALE WIRKUNG VON KALIUMPERCHLORAT

Acta Endocrinologica ◽

10.1530/acta.0.0390423 ◽

1962 ◽

Vol 39 (3) ◽

pp. 423-430

Author(s):

H. L. Krüskemper ◽

F. J. Kessler ◽

E. Steinkrüger

Keyword(s):

Thyroid Gland ◽

Male Rats ◽

Normal Male ◽

Tissue Respiration ◽

List Type ◽

Morphological Alterations ◽

Hormone Synthesis ◽

Stimulation Of

ABSTRACT 1. Reserpine does not inhibit the tissue respiration of liver in normal male rats (in vitro). 2. The decrease of tissue respiration of the liver with simultaneous morphological stimulation of the thyroid gland after long administration of reserpine is due to a minute inhibition of the hormone synthesis in the thyroid gland. 3. The morphological alterations of the thyroid in experimental hypothyroidism due to perchlorate can not be prevented with reserpine.

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HEMMUNG VON TESTOSTERONABHÄNGIGEN DIFFERENZIERUNGSVORGÄNGEN DER MÄNNLICHEN RATTE NACH DER GEBURT

Acta Endocrinologica ◽

10.1530/acta.0.0540227 ◽

1967 ◽

Vol 54 (2) ◽

pp. 227-240 ◽

Cited By ~ 19

Author(s):

F. Neumann ◽

J. D. Hahn ◽

M. Kramer

Keyword(s):

Sexual Behaviour ◽

Cyproterone Acetate ◽

Post Partum ◽

Sexual Attitude ◽

Normal Male ◽

Corpora Lutea ◽

List Type ◽

Newborn Rats ◽

Neonatal Treatment ◽

Triangular Shape

ABSTRACT Male newborn rats were injected with 2 mg of an antiandrogen (1,2α-methylene-6-chloro-pregna-4,6-dien-17α-ol-3,20-dione-17α-acetate = cyproterone acetate) daily from their 1st to their 14th day of life. The following effects of this treatment were observed in these animals after onset of sexual maturity: 84% of the animals are unable to reproduce. Penis: the frenulum is broadened to a lamina of triangular shape, which almost completely prevents the preputium from being pushed back. These males show a rather insufficient male sexual behaviour towards females in oestrus. After castration and ovar implantation, some of the treated animals show true corpora lutea and at attempts of cohabitation partially female sexual behaviour towards normal male animals. From these results it can be concluded, that differentiation of the penis is not completed at the time of birth. The infertility of the animals may be caused by the penile changes (difficulties with intromission) as well as by the aimless sexual behaviour. This aimless sexual behaviour, the ability to produce true corpora lutea and finally their partially female sexual attitude under the influence of the hormones from the implanted ovaries led to the conclusion, that the above described neonatal treatment apparently inhibited testosterone-depending post partum developments of sexual differentiation in hypothalamic centers.

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Certain aspects of the host-parasite relationship ofNematospiroides dubius(Baylis). II. The effect of sex on experimental infections in the rat (an abnormal host)

Parasitology ◽

10.1017/s0031182000070748 ◽

1961 ◽

Vol 51 (3-4) ◽

pp. 499-510 ◽

Cited By ~ 30

Author(s):

Colin Dobson

Keyword(s):

Male Rats ◽

Male Rat ◽

Normal Male ◽

Female Rat ◽

Castrate Male ◽

Parasite Relationship ◽

Nematospiroides Dubius ◽

Resistance To Infection ◽

Host Parasite ◽

The Relationship

1. The male rat is more susceptible to infections ofNematospiroides dubiusthan the female. As the rat grows older the resistance of the female rat to infection increases at a greater rate than that of the male.2. The course of the infection is modified by the sex of the host.3. More larvae penetrated the intestinal mucosa to encyst in the male than in the female. More larvae, however, formed cysts in the female than in the male rat by the fifth day.4. The male harboured more adult worms than the female rat, although this difference was not significant in the immature animals.5. The sex resistance of the rat toN. dubiusinfections was removed by bilateral gonadectomy. Castration decreased the susceptibility of the male rat, while spaying increased it in the female compared with the susceptibility in the respective normal hosts.6. Subsequent replacement of the homologous sex hormone in the gonadectomized rat restores the sex resistance, and may even increase it (particularly in the immature animals). Oestradiol increased the resistance of the spayed female rat, while testosterone increased the susceptibility of the castrate male rat to infection.7. Oestradiol implanted in castrate male rats increased the resistance of these hosts to a greater level than was shown in the normal male rat.8. The rat shows a marked age resistance over which the sex resistance is superimposed.9. The relationship between the sex of the host and its resistance to infection is discussed.This work was done during the tenure of a Department of Scientific and Industrial Research Studentship. My thanks are due to Dr E. T. B. Francis for his helpful and critical supervision and to Professor I. Chester Jones, in whose department the work was done, for the facilities he provided.

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