Anti-inflammatory effect of Phellinus linteus grown on germinated brown rice on dextran sodium sulfate-induced acute colitis in mice and LPS-activated macrophages

Abstract Salvia miltiorrhiza (SM, or Danshen) extract has been approved by China FDA for the treatment of cardiovascular and cerebrovascular diseases owing to its potent anti-inflammatory effects. Whether SM may be used to treat inflammatory bowel disease (IBD) remains elusive. In the current study, Dextran-Sodium-Sulfate (DSS) induced colitis in mice was used as a model of IBD, and SM was given orally for 7 days. SM administration has significantly reduced the disease activity index (DAI) score and weight lost and colon shortening in the DSS-induced colitis mice. The macrophage infiltration was significantly reduced in the SM treatment group. To explore the mechanisms, macrophage processor cell line Raw 264.7 was used to verify the anti-inflammatory effect of SM. SM treatment inhibited lipopolysaccharide (LPS)-induced macrophage activation in RAW264.7 cells and significantly reduced the production of pro-inflammatory factors. The current study provided evidence that oral administration of SM ameliorates pathological deterioration of IBD in mice, and warrants future clinical application of SM for the management of IBD.

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miR-135a Protects Dextran Sodium Sulfate-Induced Inflammation in Human Colorectal Cell Lines by Activating STAT3 Signal

Cellular Physiology and Biochemistry ◽

10.1159/000495481 ◽

2018 ◽

Vol 51 (3) ◽

pp. 1001-1012 ◽

Cited By ~ 4

Author(s):

Jingru Zhang ◽

Bo Lian ◽

Yan Shang ◽

Chun Li ◽

Qingkai Meng

Keyword(s):

Proinflammatory Cytokines ◽

Sodium Sulfate ◽

Inflammatory Responses ◽

Dextran Sodium Sulfate ◽

Stat3 Phosphorylation ◽

Colorectal Cell ◽

Anti Inflammatory ◽

Colonic Cells ◽

Ht 29 ◽

Inflammatory Effect

Background/Aims: miR-135a is reduced in several cancers and has been suggested to mediate immune and inflammatory responses. However, the effect of miR-135a on inflammatory bowel diseases was obscure. This study firstly attempted to investigate the hypothesis that miR-135a alleviates dextran sodium sulfate (DSS)-induced inflammation in colonic cells and potential mechanisms are also studied. Methods: Caco-2 and HT-29 cells in this study were treated with DSS, miR-135a mimic, and S3I-201, and then CKK-8 assay was used to test cell viability. Expressions of miR-135a, cytokines, and signal transducers and activators of transcription factors (STATs) were determined by RT-PCR. Also, cytokine productions were further tested by using ELISA kits. Activation or inactivation of STAT3 signal was validated by western blotting analysis. Results: The results showed that DSS markedly downregulated miR-135a expression (P< 0.05) and induced inflammatory response in Caco-2 and HT-29 cells evidenced by the up regulations and productions of interleukin-1β (IL-1β) and tumor necrosis factor-ɑ (TNF-ɑ) (P< 0.05). Transfection with miR-135a mimic significantly alleviated DSS-induced upregulation and productions of IL-1β and TNF-ɑ in Caco-2 and HT-29 cells (P< 0.05). STATs were analyzed and miR-135a mimic treatment reversed STAT3 downregulation in DSS-challenged Caco-2 and HT-29 cells compared with the mimic control (P< 0.05). Also, STAT3 phosphorylation was inhibited in DSS-challenged Caco-2 cells and miR-135a mimic activated STAT3 signal (P< 0.05). S3I-201, an inhibitor of STAT3 signal, further used to inactivate STAT3 signal and the results showed that S3I-201 blocked the anti-inflammatory effect of miR-135a mimic on Caco-2 and HT-29 cells evidenced by the lowered expressions and productions of proinflammatory cytokines ((IL-1β and TNF-ɑ) (P< 0.05). Conclusion: Our results indicated that miR-135a alleviated DSS-induced inflammation and activated STAT3 signal in colonic cells. Inhibition of STAT3 reversed the anti-inflammatory function of miR-135a by regulating proinflammatory cytokines. Thus, STAT3 signal might serve, at least in part, as the potential mechanism of miR-135a-mediated anti-inflammatory effect in colonic cells.

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Adding Germinated Brown Rice Soaked in a Mycelial Culture Broth of Phellinus linteus to Muffins: An Assessment Using the Response Surface Methodology

Journal of the Korean Society of Food Science and Nutrition ◽

10.3746/jkfn.2011.40.6.892 ◽

2011 ◽

Vol 40 (6) ◽

pp. 892-902 ◽

Cited By ~ 3

Author(s):

Kyong-Im Jung ◽

Eun-Kyung Cho

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Brown Rice ◽

Culture Broth ◽

Phellinus Linteus ◽

Germinated Brown Rice ◽

Mycelial Culture

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Heme oxygenase-1 mediates the anti-inflammatory effect of mushroom Phellinus linteus in LPS-stimulated RAW264.7 macrophages

Journal of Ethnopharmacology ◽

10.1016/j.jep.2006.01.009 ◽

2006 ◽

Vol 106 (3) ◽

pp. 364-371 ◽

Cited By ~ 44

Author(s):

Byung-Chul Kim ◽

Joung-Woo Choi ◽

Hye-Young Hong ◽

Sin-Ae Lee ◽

Suntaek Hong ◽

...

Keyword(s):

Heme Oxygenase ◽

Heme Oxygenase 1 ◽

Phellinus Linteus ◽

Raw264.7 Macrophages ◽

Anti Inflammatory ◽

Inflammatory Effect

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THE EFFECT OF MAHKOTA DEWA (PHALERIA MACROCARPA) (SCHEFF.) FRUIT PERICARP EXTRACT ON INOS IN MICE COLON INTERMITTENTLY-INDUCED BY DEXTRAN SODIUM SULFATE

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i12.20560 ◽

2017 ◽

Vol 10 (12) ◽

pp. 309

Author(s):

Kusmardi Kusmardi ◽

Dilla Shavera ◽

Ari Estuningtyas ◽

Aryo Tedjo ◽

Bambang Priyosoeryanto

Keyword(s):

Sodium Sulfate ◽

Total Yield ◽

Ethanolic Extract ◽

Dextran Sodium Sulfate ◽

Inflammatory Activity ◽

Fruit Pericarp ◽

Phaleria Macrocarpa ◽

Anti Inflammatory ◽

Oxide Synthase ◽

Different Doses

Objective: The objective of this research was to investigate the anti-inflammatory effect of Mahkota Dewa fruit pericarp extract (Phaleria macrocarpa) on inducible nitric oxide synthase (iNOS) in mice colon induced by dextran sodium sulfate (DSS).Method: The simplisia of P. macrocarpa pericarp was weighed (1000 g) and extracted by maceration process. The total yield of the ethanolic extract was 26.43%. Phytochemical screening was carried out for the detection of the phytoconstituents by simple qualitative methods. The anti-inflammatory activity was performed by DSS-induced colitis model through assessment of hematoxylin-eosin staining and expression of iNOS by immunohistochemistry assay at four different doses, i.e., 650, 1250, 2500, and 5000 mg/kg. Swiss Webster male mice weighing 25-30 g were used for the study.Results: Inflammation score in dose 625, 1250, 2500, and 5000 mg/kg were 1.63, 1.43, 1.32, and 2.20, respectively. This result is significantly different (p=0.008) with DSS group that was 4.37. The results of iNOS optical density score in dose 625, 1250, 2500, and 5000 mg/kg were 1.21, 1.119, 1.22, and 1.37, respectively. This result was significantly different (p=0.000) with DSS group that was 2.24.Conclusion: Pericarp extract of P. macrocarpa fruit exhibited anti-inflammatory activity in the experimental model shown by suppressing the expression of inflammatory cell and iNOS.

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