Anti- Deinagkistrodon acutus venom properties of ethanolic root extract from Cynanchum paniculatum (Bunge) kitag and its GC-MS analysis

2018 ◽  
Vol 225 ◽  
pp. 189-197 ◽  
Author(s):  
Yan Xiong ◽  
Bo Li ◽  
Dachun Huang ◽  
Qiyi He ◽  
Xiaodong Yu
Keyword(s):  
Root Extract ◽  
Ms Analysis ◽  
Cynanchum Paniculatum ◽  
Deinagkistrodon Acutus

FTIR, GC-MS and HPLC analysis of Baliospermum montanum (Willd.) Muell. Arg.

2020 ◽  
Vol 11 (4) ◽  
pp. 5059-5066
Author(s):  
Sushma B K ◽  
Raveesha H R
Keyword(s):  
Acetic Acid ◽  
Quantitative Estimation ◽  
Chemical Constituents ◽  
Standard Procedure ◽  
Naphthalene Acetic Acid ◽  
Root Extract ◽  
Pentanoic Acid ◽  
Trimethylsilyl Ester ◽  
Isolation And Characterization ◽  
Ms Analysis

The present work is aimed to determine the chemical constituents in Baliospermum montanum methanolic extracts. An in vitro regenerated procedure was developed for the induction of callus from stem explant cultured on Murashige and Skoog (MS) medium fortified with various concentration and permutations of 2, 4-dichloro phenoxy acetic acid, 1-naphthalene acetic acid, 6-benzyl amino purine and gibberellic acid. FTIR & GC-MS analysis was done according to standard procedure. The quantitative estimation of β-sitosterol was done by HPLC method. Maximum fresh and dry weight of callus was estimated in the combination of GA3 (0.5 mg/L) + NAA (2 mg/L) compared to other concentration. The FTIR analysis showed various functional compounds with different characteristic peak values in the extracts. Major bioactive constituents were recognized in the GC-MS analysis. Root extract revealed the existence of 1-hexadecanol, pentanoic acid, 2-(aminooxy)- and 1-hexacosanol. Leaf extract showed the presence of propanoic acid, 2-oxo-, trimethylsilyl ester, 9,12-octadecadienoic acid (z,z)-, trimethylsilyl ester, docosane, 1,22-dibromo- and pentatriacontane. Stem and stem derived callus exhibit the presence of 1,6,3,4-dihydro-2-deoxy-beta-d-lyxo-hexopyranose, n-hexadecanoic acid and pentanoic acid, 2-(aminooxy). The methanolic extract of leaf exhibited 0.2149 % of β-sitosterol content. There were no peaks observed in the root, stem and stem derived callus. Further studies are necessary for the isolation and characterization of bioactive compounds from B. montanum.

scholarly journals GC-MS ANALYSIS AND ANTIFUNGAL ACTIVITY OF METHANOLIC ROOT EXTRACT OF Chenopodium album AGAINST Sclerotium rolfsii

2017 ◽  
Vol 35 (0) ◽  
Author(s):  
A. ALI ◽  
A. JAVAID ◽  
A. SHOAIB
Keyword(s):  
Antifungal Activity ◽  
Methyl Ester ◽  
Environmental Pollution ◽  
Chenopodium Album ◽  
Octadecenoic Acid ◽  
Sclerotium Rolfsii ◽  
Gas Chromatography Mass Spectrometry ◽  
Root Extract ◽  
Malt Extract ◽  
Ms Analysis

ABSTRACT Sclerotium rolfsii is a soil-borne fungal plant pathogen that causes diseases in more than 500 plant species. Chemical fungicides used to control this disease cause environmental pollution, therefore, plant derived compounds can be used as alternative to synthetic fungicides to reduce environmental pollution. Chenopodium album is a weed of family Chenopodiaceae that is used as food and also has medicinal importance. In the present study, antifungal activity of methanolic root extract of C. album was evaluated against S. rolfsii using six concentrations viz. 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 g 100 mL-1 amended in malt extract as growth medium. All the root extract concentrations significantly reduced fungal biomass by 15-58% over control. Gas chromatography-mass spectrometry (GC-MS) analysis of the methanolic root extract of C. album was performed. Six compounds were identified in methanolic root extract through GC-MS analysis. The most abundant compound was 1,2-benzenedicarboxylic acid, mono(2-ethylhexyl) ester (58.56%) followed by 9-octadecenoic acid (Z)-, methyl ester (12.75%) and 9-octadecenoic acid (Z)-, methyl ester (10.27%), which might be responsible for antifungal activity of methanolic root extract of C. album.

GC-MS Analysis and Assessment of Antioxidant and Anthelmintic Potential of the Ethanolic Root Extract of Borassus flabellifer Linn.

2020 ◽  
Vol 10 (4) ◽  
pp. 150-155
Author(s):  
Lakshmi Kanta Kanthal ◽  
Prasenjit Mondal ◽  
Rekhabai Thakur ◽  
Surajit Maity ◽  
Palepu Kavi ◽  
...  
Keyword(s):  
Root Extract ◽  
Ms Analysis

scholarly journals In vitro neutralization of Eachis carinatus and Naja naja venom by Canthium parviflorum and its GC-MS analysis

2016 ◽  
Vol 11 (4) ◽  
pp. 920 ◽  
Author(s):  
Vineetha M. Shrikanth ◽  
Bhavya Janardhan ◽  
Sunil S. More
Keyword(s):  
Phospholipase A2 ◽  
Enzyme Activities ◽  
Methanol Extract ◽  
Video Clip ◽  
Hydrolytic Enzymes ◽  
Root Extract ◽  
Site Specific ◽  
Naja Naja ◽  
Ms Analysis

<p class="Abstract"><em>Canthium </em>parviflorum is used in the traditional therapy to treat snakebite victims. In the present study, partially purified methanol root extract of <em>C. </em>parviflorum was used for the neutralization of Eachis carinatus and <em>Naja naja</em> venom. The extract inhibited <em>in vitro</em> phosphomonoesterase, phosphodiesterase, acetylcholinesterase, hyaluronidase, protease, phospholipase A<sub>2 </sub>and 5’nucleotidase activities of both venoms. One of the promising band exhibited neutralization of all the<em> in vitro</em> enzyme activities and was further subjected to GC-MS analysis which revealed the presence of eight active phytocompounds. These phytochemicals might be responsible for <em>in vitro</em> enzyme neutralization. Methanol extract possesses potent active phytochemicals against the site specific toxins and hydrolytic enzymes analyzed.</p><p class="Abstract"><strong>Video Clip of Methodology</strong>:</p><p class="Abstract"><a href="https://youtube.com/v/oSLdFcfaJxU">Phospholipase A2 assay</a>: 1 min 36 sec</p>

scholarly journals PHYTOCHEMICAL ANALYSIS AND IN VITRO STUDIES ON ANTIBACTERIAL, ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES USING CASUARINA EQUISETIFOLIA BARK EXTRACTS

2018 ◽  
Vol 10 (1) ◽  
pp. 118
Author(s):  
Saranya V. T. K. ◽  
S. Uma Gowrie
Keyword(s):  
Secondary Metabolites ◽  
Tree Species ◽  
Forest Tree ◽  
Root Extract ◽  
Dependent Manner ◽  
Casuarina Equisetifolia ◽  
Forest Tree Species ◽  
Ms Analysis ◽  
Anti Inflammatory

Objective: Casuarina equisetifolia is an important multipurpose exotic forest tree species widely cultivated in the coastal regions of Tamil Nadu that serves as a warehouse of essential secondary metabolites. Identification of these bioactive compounds in this forest tree species might lead to the discovery and development of a new drug to treat various diseases. Methods: The present study was carried out with an objective to analyse the phytochemicals qualitatively and quantitatively. Gas Chromatography-Mass Spectrometry (GC-MS) analysis was performed to evaluate the presence of various volatile compounds. An in vitro antibacterial, antioxidant and anti-inflammatory properties of aqueous and organic solvents of C. equisetifolia bark was studied.Results: The preliminary qualitative screening revealed the presence of alkaloids, glycosides, carbohydrates, proteins, flavonoids, phenols, terpenoids, and tannins. The quantitative analysis revealed the presence of maximum phenols (71.2±0.51 mg/g), flavonoids (35.12±0.34 mg/g), tannins (77.59±0.21) and terpenoids (6%) in methanolic root extract with respective standards. Several peaks were obtained in the GC-MS analysis which indicates the presence of different secondary metabolites. Antibacterial activity showed a maximum zone of inhibition against Escherichia coli (23±0.24 mm) and Proteus vulgaris (23±0.32 mm). The antioxidant potential of various extracts was compared with the standard ascorbic acid. Anti-inflammatory activity was compared with standard diclofenac sodium and the extract showed activities significantly in a dose-dependent manner. Conclusion: From this study, it is revealed that C. equisetifolia bark extract possesses efficient antibacterial property, the potential in scavenging free radical, effective antioxidant, powerful anti-inflammatory source that can be employed in the development of a novel drug to treat various diseases.

scholarly journals Screening and Evaluation of Bioactive Components of Methanol Root Extract of Anthocleista nobilis G. Don. by GCMS Analysis

2020 ◽  
Author(s):  
Adekunle ORIMISAN Ojatula
Keyword(s):  
Methanol Extract ◽  
Biological Activities ◽  
Biologically Active ◽  
Root Extract ◽  
Bioactive Constituents ◽  
Active Metabolites ◽  
Ms Analysis ◽  
Medicinal Value ◽  
Study Results ◽  
Phytochemical Compounds

Abstract Background: Medicinal plants have great importance in African medicine and are also used as precursors in drug discovery. The medicinal value of plants lies in their bioactive constituents which usually allow them to fight against several diseases. Plant-based natural constituents can be derived from any part of the plant like roots, bark, leaves, flowers, fruits, seeds. The present study was designed to determine the bioactive/biochemical compounds present in the methanol extract of Anthocleista nobilis root.Materials and Methods: GC -MS analysis of the methanol extract of A. nobilis root was performed using a Perkin Elmer GC Clarus 500 system comprising an Agilent technologies 5975 MSD model detector and a gas chromatograph interfaced to a mass spectrometer with the aid of the Turbo mass 5.0 software.Results: The study results of the GC-MS analysis provided different phytochemical compounds possessing several biological activities such as antimicrobial, antifungal, anticancer, and anti-inflammatory activities. This study therefore, showed that root of Anthocleista nobilis is a source of biologically active metabolites. Furthermore, root extract revealed the presence of diverse chemical constituents.Conclusion: The results of the present study suggest a recommendation of A. nobilis root as a plant of phytopharmaceutical importance.

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