scholarly journals Screening and Evaluation of Bioactive Components of Methanol Root Extract of Anthocleista nobilis G. Don. by GCMS Analysis

2020 ◽  
Author(s):  
Adekunle ORIMISAN Ojatula
Keyword(s):  
Methanol Extract ◽  
Biological Activities ◽  
Biologically Active ◽  
Root Extract ◽  
Bioactive Constituents ◽  
Active Metabolites ◽  
Ms Analysis ◽  
Medicinal Value ◽  
Study Results ◽  
Phytochemical Compounds

Abstract Background: Medicinal plants have great importance in African medicine and are also used as precursors in drug discovery. The medicinal value of plants lies in their bioactive constituents which usually allow them to fight against several diseases. Plant-based natural constituents can be derived from any part of the plant like roots, bark, leaves, flowers, fruits, seeds. The present study was designed to determine the bioactive/biochemical compounds present in the methanol extract of Anthocleista nobilis root.Materials and Methods: GC -MS analysis of the methanol extract of A. nobilis root was performed using a Perkin Elmer GC Clarus 500 system comprising an Agilent technologies 5975 MSD model detector and a gas chromatograph interfaced to a mass spectrometer with the aid of the Turbo mass 5.0 software.Results: The study results of the GC-MS analysis provided different phytochemical compounds possessing several biological activities such as antimicrobial, antifungal, anticancer, and anti-inflammatory activities. This study therefore, showed that root of Anthocleista nobilis is a source of biologically active metabolites. Furthermore, root extract revealed the presence of diverse chemical constituents.Conclusion: The results of the present study suggest a recommendation of A. nobilis root as a plant of phytopharmaceutical importance.

scholarly journals ANTIMICROBIAL AND OTHER BIOACTIVE CONSTITUENTS OF Cannabis sativus ROOTS FROM PAKISTAN

2021 ◽  
Vol 27 (3) ◽  
pp. 359-368
Author(s):  
Arshad Javaid ◽  
Iqra Haider Khan ◽  
Malik F.H. Ferdosi
Keyword(s):  
Methyl Ester ◽  
Cannabis Sativa ◽  
Biological Activities ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Biologically Active ◽  
Root Extract ◽  
Bioactive Constituents ◽  
Acid Methyl ◽  
Tetracosanoic Acid

Hemp (Cannabis sativa L.), a medicinal plant of family Cannabaceae, growing wildly in most of the rainfed areas of Punjab, Pakistan. In this study, phytochemical profile of methanolic extract of root was assessed through GC-MS analysis and various biologically active compounds were identified through literature survey. There were 14 compounds in the root extract. The most abundant compounds were γ-sitosterol (27.08%) and 9,12-octadecadienoic acid (Z,Z)-, methyl ester (24.09%) and hexadecanoic acid, methyl ester (21.81%). Other identified compounds were methyl stearate (5.51%), stigmasterol (5.12%), campesterol (4.19%), phenol, 2,2'-methylenebis[6-(1,1-dimethylethyl)-4-methyl-(2.52%), 11-octadecenoic acid, methyl ester (2.10%), squalene (1.89%), β-amyrin (1.88%), eicosanoic acid, methyl ester (0.85%), tetracosanoic acid, methyl ester (0.88%), dronabinol (1.02%) and 2-methoxy-4-vinylphenol (0.96%). Most of the identified compounds possess one or more biological activities viz. antitumor, antifungal, antibacterial, antioxidant, anticancer, anti-inflammatory, antidiabetic, and/or analgesic.

scholarly journals GC-MS Analysis of Spondias mombin (Linn) Methanol Leaf Extract

2021 ◽  
pp. 1-7
Author(s):  
S. C. Okereke ◽  
O. V. Ikpeazu ◽  
C. J. Nwaogwugwu ◽  
A. S. Ezekwe ◽  
C. I. Nosiri ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Molecular Formula ◽  
Spondias Mombin ◽  
Ms Analysis ◽  
Medicinal Value ◽  
Bioactive Component ◽  
Phytochemical Compounds ◽  
Molecular Masses

The bioactive components of Spondias mombin leaf have been evaluated using gas chromatography and mass spectroscopy (GC/MS). The plant were collected washed, shade dried and milled and the methanol extract of the leaf plant were prepared by soxhlet extract method. The methanol extract were analyzed for the identification of phytochemical compounds presenting the Spondias mombin plant leaf using GC/MS methods. Result of the GC-MS analysis of Spondias mombin leaf extract leads to the identification of 8 compounds. The identification of phytochemicals was based on the Molecular Weight, molecular formula, RT (Retention Time) and peak area % (concentration). These peaks indicated the presence of eight bioactive compounds in the extract. The names, molecular formular, percentage composition and molecular masses of these compounds are shown in Table 1. The 8 bioactive compounds are as follows; 9- oxononanoic acid,3-isopropoxy, 1,1,15,5,5 hexamethyl-3- trimethylsioxytrosiloxane (C12H34O4Si4) ,1,4-methanocycloocta(d)pyridazine, 1α4α4aα10α)(C13H2ON2),Hexasiloxane 1,1,3,3,5,5,7,7,9,9,11,11-dodeca methyl,1,4 methanocycloocta (d) pyridazine 1,4,4a,5,6,9,10,10a- octalydro 11,11-dimethyl-(1α,4α4aα10α), Hexasiloxane, 1,1,3,3,5,5,7,7,9,9,11,11 dodecamethyl, 3- isopropoxy-1,1,1,5,5,5 hexamethyl-3-(trimethylsiloxy) trisiloxane C12H34O4Si4 and 1,1,1,3,5,5,5- Heptamethyltrisiloxane(C7H22O2Si3). It is concluded that these bioactive component are known to exhibit medicinal value as well as pharmacological activities.

Metabolomics of Healthy Berry Fruits

2019 ◽  
Vol 25 (37) ◽  
pp. 4888-4902 ◽  
Author(s):  
Gilda D'Urso ◽  
Sonia Piacente ◽  
Cosimo Pizza ◽  
Paola Montoro
Keyword(s):  
Biological Activities ◽  
Biologically Active ◽  
In Vivo Studies ◽  
Bioactive Metabolites ◽  
Active Metabolites ◽  
Positive Effects ◽  
Cell Functions ◽  
Berry Fruits

The consumption of berry-type fruits has become very popular in recent years because of their positive effects on human health. Berries are in fact widely known for their health-promoting benefits, including prevention of chronic disease, cardiovascular disease and cancer. Berries are a rich source of bioactive metabolites, such as vitamins, minerals, and phenolic compounds, mainly anthocyanins. Numerous in vitro and in vivo studies recognized the health effects of berries and their function as bioactive modulators of various cell functions associated with oxidative stress. Plants have one of the largest metabolome databases, with over 1200 papers on plant metabolomics published only in the last decade. Mass spectrometry (MS) and NMR (Nuclear Magnetic Resonance) are the most important analytical technologies on which the emerging ''omics'' approaches are based. They may provide detection and quantization of thousands of biologically active metabolites from a tissue, working in a ''global'' or ''targeted'' manner, down to ultra-trace levels. In the present review, we highlighted the use of MS and NMR-based strategies and Multivariate Data Analysis for the valorization of berries known for their biological activities, important as food and often used in the preparation of nutraceutical formulations.

scholarly journals In vitro neutralization of Eachis carinatus and Naja naja venom by Canthium parviflorum and its GC-MS analysis

2016 ◽  
Vol 11 (4) ◽  
pp. 920 ◽  
Author(s):  
Vineetha M. Shrikanth ◽  
Bhavya Janardhan ◽  
Sunil S. More
Keyword(s):  
Phospholipase A2 ◽  
Enzyme Activities ◽  
Methanol Extract ◽  
Video Clip ◽  
Hydrolytic Enzymes ◽  
Root Extract ◽  
Site Specific ◽  
Naja Naja ◽  
Ms Analysis

<p class="Abstract"><em>Canthium </em>parviflorum is used in the traditional therapy to treat snakebite victims. In the present study, partially purified methanol root extract of <em>C. </em>parviflorum was used for the neutralization of Eachis carinatus and <em>Naja naja</em> venom. The extract inhibited <em>in vitro</em> phosphomonoesterase, phosphodiesterase, acetylcholinesterase, hyaluronidase, protease, phospholipase A<sub>2 </sub>and 5’nucleotidase activities of both venoms. One of the promising band exhibited neutralization of all the<em> in vitro</em> enzyme activities and was further subjected to GC-MS analysis which revealed the presence of eight active phytocompounds. These phytochemicals might be responsible for <em>in vitro</em> enzyme neutralization. Methanol extract possesses potent active phytochemicals against the site specific toxins and hydrolytic enzymes analyzed.</p><p class="Abstract"><strong>Video Clip of Methodology</strong>:</p><p class="Abstract"><a href="https://youtube.com/v/oSLdFcfaJxU">Phospholipase A2 assay</a>: 1 min 36 sec</p>

scholarly journals Preliminary phytochemical screening and GC-MS analysis of Cladophora glomerata: green marine algae

2019 ◽  
Vol 8 (4) ◽  
pp. 732
Author(s):  
Usha N. S. ◽  
Sabari Anand J. V. ◽  
Mangaiyarkarasi .
Keyword(s):  
Methanolic Extract ◽  
Animal Feed ◽  
Biological Activities ◽  
Acid Methyl Ester ◽  
Biologically Active ◽  
Mass Spectrometry Analysis ◽  
Human Consumption ◽  
Cladophora Glomerata ◽  
Asian Countries ◽  
Ms Analysis

Background: Seaweeds since ages are excellent source of biologically active ingredients. Several Asian countries have a strong tradition of using various seaweeds in herbal medicines preparations. These plants contain various phytochemical constituents having biological activities. Seaweeds are the source of phytochemicals namely agar-agar, carrageenan and algin, which are extensively used in various industries such as food, confectionary, textiles, pharmaceuticals, dairy and paper industries mostly as gelling, stabilizing and thickening agents. They are also used for human consumption, animal feed and as manure in several countries. Several Asian countries are using various seaweeds in traditional medicines.Methods: In the present study, the shade dried and methanolic extract of Cladophora glomerata, a marine green algae was subjected to preliminary phytochemical and gas chromatography-mass spectrometry analysis(GC-MS) to identify the various bioactive components.Results: The methanolic extract of Cladophora glomerata revealed the presence of alkaloids, glycosides, flavonoids, saponins, diterpenes and carbohydrates. The GC-MS analysis of the methanolic extract of Cladophora glomerata showed the presence of 42 different compounds. The major compounds were dibutyl phthalate (27.07%), hexadecanoic acid, methyl ester (9.58%), 1,2-benzene-di-carboxylic acid (8.11%), octatriacontyl trifluoroacetate (6.81%), cholesterol (6.66%).Conclusions: Thus, in the present study of Cladophora glomerata, phytochemical and GC-MS analysis provides an important novel information to support further ongoing studies to evaluate structure of bioactive compound and its pharmacological activities.

scholarly journals Metabolic fingerprinting, antioxidant characterization, and enzyme-inhibitory response of Monotheca buxifolia (Falc.) A. DC. extracts

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Joham Sarfraz Ali ◽  
Hammad Saleem ◽  
Abdul Mannan ◽  
Gokhan Zengin ◽  
Mohamad Fawzi Mahomoodally ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
Methanol Extract ◽  
Biological Activities ◽  
Chloroform Extract ◽  
Inhibitory Response ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Ms Analysis ◽  
Monotheca Buxifolia

Abstract Background Ethnobotanical and plant-based products allow for the isolation of active constituents against a number of maladies. Monotheca buxifolia is used by local communities due to its digestive and laxative properties, as well as its ability to cure liver, kidney, and urinary diseases. There is a need to explore the biological activities and chemical constituents of this medicinal plant. Methods In this work, the biochemical potential of M. buxifolia (Falc.) A. DC was explored and linked with its biological activities. Methanol and chloroform extracts from leaves and stems were investigated for total phenolic and flavonoid contents. Ultrahigh-performance liquid chromatography coupled with mass spectrometry (UHPLC–MS) was used to determine secondary-metabolite composition, while high-performance liquid chromatography coupled with photodiode array detection (HPLC–PDA) was used for polyphenolic quantification. In addition, we carried out in vitro assays to determine antioxidant potential and the enzyme-inhibitory response of M. buxifolia extracts. Results Phenolics (91 mg gallic-acid equivalent (GAE)/g) and flavonoids (48.86 mg quercetin equivalent (QE)/g) exhibited their highest concentration in the methanol extract of stems and the chloroform extract of leaves, respectively. UHPLC–MS analysis identified a number of important phytochemicals, belonging to the flavonoid, phenolic, alkaloid, and terpenoid classes of secondary metabolites. The methanol extract of leaves contained a diosgenin derivative and polygalacin D, while kaempferol and robinin were most abundant in the chloroform extract. The methanol extract of stems contained a greater peak area for diosgenin and kaempferol, whereas this was true for lucidumol A and 3-O-cis-coumaroyl maslinic acid in the chloroform extract. Rutin, epicatechin, and catechin were the main phenolics identified by HPLC–PDA analysis. The methanol extract of stems exhibited significant 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical-scavenging activities (145.18 and 279.04 mmol Trolox equivalent (TE)/g, respectively). The maximum cupric reducing antioxidant capacity (CUPRAC) (361.4 mg TE/g), ferric-reducing antioxidant power (FRAP) (247.19 mg TE/g), and total antioxidant potential (2.75 mmol TE/g) were depicted by the methanol extract of stems. The methanol extract of leaves exhibited stronger inhibition against acetylcholinesterase (AChE) and glucosidase, while the chloroform extract of stems was most active against butyrylcholinesterase (BChE) (4.27 mg galantamine equivalent (GALAE)/g). Similarly, the highest tyrosinase (140 mg kojic-acid equivalent (KAE)/g) and amylase (0.67 mmol acarbose equivalent (ACAE)/g) inhibition was observed for the methanol extract of stems. Conclusions UHPLC–MS analysis and HPLC–PDA quantification identified a number of bioactive secondary metabolites of M. buxifolia, which may be responsible for its antioxidant potential and enzyme-inhibitory response. M. buxifolia can be further explored for the isolation of its active components to be used as a drug.

Biologically active metabolites from Agelas mauritiana

1988 ◽  
Vol 66 (1) ◽  
pp. 45-50 ◽  
Author(s):  
R. Fathi-Afshar ◽  
T. M. Allen
Keyword(s):  
Spectral Data ◽  
Biological Activities ◽  
Biologically Active ◽  
Adrenergic Blockade ◽  
Active Metabolites ◽  
Antagonistic Action ◽  
Wide Range ◽  
Biologically Active Metabolites

Two novel bicyclic diterpenoides, agelasimine-A (9), and agelasimine-B (10), have been isolated from the orange sponge Agelas mauritiana. Also, a new bromine-containing alkaloid, 5-debromomidpacamide (12), along with midpacamide (13) and methyl N-methyl-4,5-dibromopyrrole-2-carboxylate (11), has been isolated. The structures were determined by interpretation of their spectral data. Agelasimine-A and -B exhibit a wide range of interesting biological activities such as cytotoxicity, inhibition of adenosine transfer into rabbit erythrocytes, Ca2+-channel antagonistic action, and α1 adrenergic blockade.

scholarly journals HPLC Analysis and Antimicrobial Screening of Methanol Extract/Fractions of the Root of Millettia aboensis (Hook.f.) Baker against Streptococcus mutans

2020 ◽  
pp. 1-11
Author(s):  
Adeniran J. Ikuesan ◽  
Eze E. Ajaegbu ◽  
Ukachukwu C. Ezeh ◽  
Adaobi J. Dieke ◽  
Adaora L. Onuora ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Methanol Extract ◽  
Extraction Methods ◽  
Antimicrobial Activities ◽  
Root Extract ◽  
Plant Materials ◽  
Aqueous Fraction ◽  
Plant Parts ◽  
Percentage Yield ◽  
Phytochemical Compounds

Millettia aboensis (Hook.f.) Baker belongs to the Leguminosae family, known locally as nduezi in Igbo, erurumesi in Edo, and Òdúdū in Efik. The plant parts have proven to be active against some diseases. The plant was studied for its antimicrobial activity and the phytochemicals present. The plant materials were macerated and fractionated using different extraction methods. The compounds present in the fractions of the stem were detected with the aid of HPLC-DAD. The efficacy of the crude methanol and fractions from the root part was evaluated against Streptococcus mutans. The results showed that the aqueous fraction extract had the highest percentage yield (51.11%), followed by the ethyl acetate (24.10%), butanol (6.63%), and hexane (5.56%), while the methanol extract had a yield of 2.48%. Nine phytochemical compounds were detected from the fractions of the root extract: bis-oxazolidinone derivative – 1, circumdatin F – 2, enniatin B – 3, septicine – 4, orobol 8-C-glucoside – 5, rocaglamide – 6, genistein 6-C-glucoside – 7, 3-phenyl chromen-4-one – 8, and corynesidone D – 9. The methanol extract/fractions of M. aboensis showed no antimicrobial activities on clinical isolates at different concentrations (6.25-50 mg/ml). Based on the poor activity against Strep. mutans, but with promising phytoconstituents present, the extract/fractions need further investigation as regards its utilization against other pathogens.

scholarly journals Algae Bioactive Constituents and Possible Role During COVID-19 Pandemic (A review)

2021 ◽  
Vol 30 (2) ◽  
pp. 16-22
Author(s):  
Sayed Rashad ◽  
Ghadir El-Chaghaby
Keyword(s):  
Bioactive Compounds ◽  
Biological Activities ◽  
Biologically Active ◽  
Bioactive Constituents ◽  
Algae Biomass ◽  
Synthetic Drugs ◽  
Pharmaceutical Applications ◽  
Active Chemical ◽  
Wide Range ◽  
Algal Products

Nowadays, the use of natural bio-products in pharmaceuticals is gaining popularity as safe alternatives to chemicals and synthetic drugs. Algal products are offering a pure, healthy and sustainable choice for pharmaceutical applications. Algae are photosynthetic microorganisms that can survive in different environmental conditions. Algae have many outstanding properties that make them excellent candidate for use in therapeutics. Algae grow in fresh and marine waters and produce in their cells a wide range of biologically active chemical compounds. These bioactive compounds are offering a great source of highly economic bio-products. The present review discusses the phytochemical and bioactive compounds present in algae biomass and their potent biological activities. The review focuses on the use of alga in therapy and their pharmaceutical applications with special reference to the possible preventive and therapeutic role of algae against COVID-19.

scholarly journals Analisis GCMS terhadap Senyawa Fitokimia Ekstrak Metanol Ganoderma lucidum

2019 ◽  
Vol 3 (2) ◽  
pp. 147
Author(s):  
Surahmaida Surahmaida ◽  
Tri Puji Lestari Sudarwati ◽  
Junairiah Junairiah
Keyword(s):  
Human Health ◽  
Ganoderma Lucidum ◽  
Methanol Extract ◽  
Biological Activities ◽  
Fine Powder ◽  
Pharmacological Effects ◽  
Phytochemical Compounds

AbstrakPenelitian ini bertujuan untuk menganalisis senyawa fitokimia ekstrak metanol Ganoderma lucidum dengan metode GCMS. Tahapan penelitian ini meliputi  proses ekstraksi serbuk halus G. lucidum menggunakan pelarut metanol dengan metode maserasi selama 3x3 hari dan ekstrak metanol G. lucidum yang didapatkan dianalisis dengan alat GCMS. Senyawa fitokimia yang teridentifikasi oleh GCMS antara lain thiophene, 2-hexyl-; 3-((3-Acetoxythyl)-6-acetoxymethyl-2,4-dimethyl)phenyl)-2-methyl-(E)-2-propenyl acetate; 2,7-Diphenylindole; dan 5-(2-Bromophenyl)-7-chloro-2,3-dihydro-1H-1,4-benzodiazepin-2-one. Dapat disimpulkan bahwa senyawa fitokimia pada ekstrak metanol G. lucidum memiliki efek farmakologis bagi kesehatan manusia dan aktivitas biologis lainnya.  Katakunci: Ganoderma lucidum, senyawa fitokimia, GCMS AbstractThis study aims to analyze the phytochemical compounds of methanol extract of Ganoderma lucidum using the GCMS method. The stages of this study include extraction of fine powder G. lucidum using methanol solvent by maceration method for 3x3 days and methanol extract of G. lucidum obtained were analyzed by GCMS. Phytochemical compounds identified by GCMS is thiophene, 2-hexyl-; 3 - ((3-Acetoxythyl) -6-acetoxymethyl-2,4-dimethyl) phenyl) -2-methyl- (E) -2-propenyl acetate; 2,7-Diphenylindole; and 5- (2-Bromophenyl) -7-chloro-2,3-dihydro-1H-1,4-benzodiazepine-2-one. It can be concluded that phytochemical compounds in methanol extract of G. lucidum have pharmacological effects on human health and other biological activities. Keywords: Ganoderma lucidum, phytochemical compounds, GCMS

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