Bioassay-guided fractionation and identification of wound healing active compound from Pistacia vera L. hull extract.

Background: Several plants including Sida rhombifolia Linn. (Malvaceae) which are said to be used by traditional health practitioners in Tanzania for wound treatment have not yet been evaluated. The objective of this study was to investigate the ointment formulation of S. rhombifolia leaves for its potential wound healing activities.Methods: Wound healing activity of S. rhombifolia leaves was investigated in mice using 50%, 33% and 25% formulated 80% ethanolic leaves extract ointment and water preparations. Excisional and incisional wound-induction models were used with 6 albino mice in each group. The wound diameter (for contraction assessment), duration of re-epithelisation in days, percentage tensile strength as well as the degree of collagenisation and fibrosis were investigated.Results: S. rhombifolia leaves extract had significant mean percentage wound closure for all ointment formulations used and for the water preparation from day 7. A significant percentage tensile strength on day 10 for all formulations used was observed. The 50% ointment had a mean of 64.1±1.7 (p=1.2-09), 33% ointment had a mean of 64.0±3.2 (p=2.4-08) and the 25% ointment had a mean of 53.1±4.0 (p=1.3-06). A remarkable fibrosis and collagenisation for the 50% ointment and the water preparation was observed.Conclusion: The formulated ointments and the water preparations of S. rhombifolia leaves have a potential benefit in enhancing wound healing. A bioassay guided fractionation is recommended to allow identification of its active compound(s) with wound healing activity for drug development.

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Evaluation of Performance, Blood Biochemical indices and Immune Response of Broilers Fed Pistachio Hull Extract (Pistacia Vera) of Fandoghi and Kaleghochi

Research on Animal Production ◽

10.29252/rap.9.20.19 ◽

2018 ◽

Vol 9 (20) ◽

pp. 19-26

Author(s):

Hadi Yosefi ◽

Seyyed Javad Hosseini-Vashan ◽

Seyyed Ehsan Ghiasi ◽

Mohammad Hassan Namaei ◽

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...

Keyword(s):

Immune Response ◽

Pistacia Vera ◽

Blood Biochemical ◽

Evaluation Of Performance ◽

Hull Extract ◽

Biochemical Indices ◽

Blood Biochemical Indices

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Antidiabetic Potential of Prosopis farcta Roots: In Vitro Pancreatic Beta Cell Protection, Enhancement of Glucose Consumption, and Bioassay-Guided Fractionation

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/8048273 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9 ◽

Cited By ~ 2

Author(s):

Behzad Shahbazi ◽

Saba Feyzmand ◽

Fataneh Jafari ◽

Nastaran Ghiasvand ◽

Gholamreza Bahrami ◽

...

Keyword(s):

Active Compound ◽

Pancreatic Beta Cell ◽

Glucose Consumption ◽

Fold Increase ◽

Active Fraction ◽

Cell Protection ◽

Prosopis Farcta ◽

Bioassay Guided Fractionation ◽

Beta Cell Protection

By using the streptozotocin- (STZ-) induced cytotoxicity in β-TC3 cells as an assay model, a bioassay-guided fractionation study was employed to isolate and characterize the potential antidiabetic principles of roots of Prosopis farcta. A combination of open column chromatography on reverse-phase silica gel using a water-ethanol gradient (10 : 90 to 100 : 0) followed by HPLC-based fractionation led to an active compound that appears to be composed of carbohydrate/sugar. When cell viability under STZ was reduced to 49.8 ± 4% (mean ± SD), treatment with the active compound at the concentration of 0.5 mg/mL either as a coadministration or a pretreatment improved the viability to 93 ± 1.9% and 91.5 ± 7%, respectively. The reduction in the mitochondrial membrane potential by STZ (47.34 ± 8.9% of control) was similarly recovered to 84.5 ± 4.3 (coadministration) and 88 ± 5.5% (pretreatment) by the active fraction. The bioassay-guided fractionation, β-cell protective effect, and increased glucose consumption (up to 1.49-fold increase) in hepatocytes by the extracts and active fraction are also discussed.

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