scholarly journals Evaluation of the wound healing activity of formulated ointments and water preparation from Sida rhombifolia leaf extract

2018 ◽  
Vol 20 (4) ◽  
Author(s):  
Patrick Francis ◽  
Pax J. Masimba ◽  
Amos R. Mwakigonja
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wound Closure ◽  
Wound Induction ◽  
Traditional Health ◽  
Health Practitioners ◽  
Ointment Formulation ◽  
Bioassay Guided Fractionation ◽  
Sida Rhombifolia ◽  
Wound Healing Activity

Background: Several plants including Sida rhombifolia Linn. (Malvaceae) which are said to be used by traditional health practitioners in Tanzania for wound treatment have not yet been evaluated. The objective of this study was to investigate the ointment formulation of S. rhombifolia leaves for its potential wound healing activities.Methods: Wound healing activity of S. rhombifolia leaves was investigated in mice using 50%, 33% and 25% formulated 80% ethanolic leaves extract ointment and water preparations. Excisional and incisional wound-induction models were used with 6 albino mice in each group. The wound diameter (for contraction assessment), duration of re-epithelisation in days, percentage tensile strength as well as the degree of collagenisation and fibrosis were investigated.Results: S. rhombifolia leaves extract had significant mean percentage wound closure for all ointment formulations used and for the water preparation from day 7. A significant percentage tensile strength on day 10 for all formulations used was observed. The 50% ointment had a mean of 64.1±1.7 (p=1.2-09), 33% ointment had a mean of 64.0±3.2 (p=2.4-08) and the 25% ointment had a mean of 53.1±4.0 (p=1.3-06). A remarkable fibrosis and collagenisation for the 50% ointment and the water preparation was observed.Conclusion: The formulated ointments and the water preparations of S. rhombifolia leaves have a potential benefit in enhancing wound healing. A bioassay guided fractionation is recommended to allow identification of its active compound(s) with wound healing activity for drug development.

Comparison of the ficus for the wound healing activity in various species

2020 ◽  
Vol 10 (4) ◽  
pp. 67-70
Author(s):  
Mothilal K ◽  
Akila CR ◽  
Mahender K ◽  
Chaitanya Kumar K ◽  
Ravi D
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Subcutaneous Tissue ◽  
Significant Activity ◽  
Herbal Extracts ◽  
Standard Drug ◽  
Excision Wound ◽  
Injuries And Wounds ◽  
Skin Conditions ◽  
Wound Healing Activity

Injuries and wounds are any sorts of damage to the skin or subcutaneous tissue. Usually, any wounds of such sorts are self-healed. Sometimes, there may be a delay in healing, and that delay is caused due to the functional delays in various processes of wound healing. All the Ficus plants show similar activities like the antioxidant, anti-inflammatory and wound healing properties 7including skin conditions like ulcers and rheumatism. The anthelmintic property and immunomodulatory are also seen. The herbal extracts of the same family of Ficus in different plants were investigated for the wound healing activity in the excision wound method, and the extracts showed significant activity compared to the drug. All the extracts showed a better healing ability, but the extract of FBO-100 showed the highest activity followed by FMO followed by FHO and finally the FRO. Overall, the activity of the extract ointment was comparable and was significant compared to the standard drug ointment. The wound closure of the extract ointment treated groups were better and were completed in 12 days, and the activity was more than 96%. The herbal extracts of the same family of Ficus in different plants were investigated for the wound healing activity in the excision wound method, and the extracts showed significant activity compared to the drug. The plants of microcarpa, benghalensis, religiosa and hispida are compared for the activity, and the order showed for the activity was FBO>FMO>FHO>FRO.

EVALUATION OF EXCISIONAL AND INCISIONAL WOUND HEALING ACTIVITY OF ELECTROHOMEOPATHIC DRUG (SPAGYRIC ESSENCE) GREEN ELECTRICITY IN RATS

2020 ◽  
pp. 72-75
Author(s):  
PRASANT KUMAR SABAT ◽  
SWETA PRIYADARSINI PRADHAN ◽  
RANDEEP PATRO
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wound Contraction ◽  
Control Group ◽  
Albino Rats ◽  
Green Electricity ◽  
Wound Model ◽  
Wound Protection ◽  
Incision Wound ◽  
Wound Healing Activity

Objective: The current research is an attempt to evaluate the wound healing activity of Electrohomeopathic drug Green electricity (GE) in Wistar rats. No data have been reported till date on the wound healing effect of GE. Hence the present research focuses on the scientific investigation of the wound healing activity of GE in rats. Methods: Excision and incision wounds were inflicted upon albino rats. In the excision wound model, the wound contraction, Scar residue, area and time of complete epithelization were measured subsequently on day 0, 3,6,9,12,15, 18 and 21 after inflicting the wound. Wound contraction was calculated as a percentage change in the initial wound size. In the incision wound model, the tensile strength was measured on the 10th post wounding day. Results: It was noted that the effect produced by the GE Showed significant wound healing (p<0.01) in all wound models when compared to the control group. In the case of the excision model, GE statistically increases the wound contraction rate and in the case of the incision model GE showed high tensile strength as compared to the control group. In both cases the epithelization period was significantly decreased as compared to control. Almost 100% wound protection was achieved by GE in 21 d as compared to control. Conclusion: GE (ointment) showed significant wound healing activity (p<0.01) in both the excised wound model and incision wound model in rats.

WOUND HEALING ACTIVITY OF THE LEAVES OF WRIGHTIA TINCTORIA (ROXB) R. BR

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (03) ◽  
pp. 40-46
Author(s):  
M. C. Divakar ◽  
◽  
S Lakshmi Devi
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Excision Wound ◽  
Wound Model ◽  
Methanolic Extracts ◽  
Wrightia Tinctoria ◽  
Incision Wound ◽  
Wound Healing Activity

The study was aimed to evaluate wound healing activity of 70% ethanolic and methanolic extracts of leaves of Wrightia tinctoria (Roxb) R. Br (Apocynaceae) using incision and excision wound models on Wistar rats. Wound contraction and period of epithelization were assessed in excision wound model whereas wound tensile strength was determined in case of incision wound model. 4% Wrightia tinctoria methanolic leaf extract phytosome exhibited significant wound healing potential when compared with standard 0.2% nitrofurazone ointment.

AN in vitro STUDY OF WOUND HEALING ACTIVITY OF Ficus deltoidea LEAF EXTRACT

2015 ◽  
Vol 77 (3) ◽  
Author(s):  
Nurul Ain Aqilla Wan Mustaffa ◽  
Rosnani Hasham ◽  
Mohamad Roji Sarmidi
Keyword(s):  
Wound Healing ◽  
Leaf Extract ◽  
Wound Closure ◽  
In Vitro Study ◽  
Human Skin Fibroblast ◽  
Dependent Manner ◽  
Ficus Deltoidea ◽  
Migration Assay ◽  
Wound Healing Activity

Ficus deltoidea (Mas Cotek) is one of the popular herbs that has been used traditionally to alleviate illnesses. The present study aimed to investigate the in vitro wound healing activity of  F. deltoidea leaf extract on skin cell. Cell proliferative and migration assay were done on the Human Skin Fibroblast cell (HSF 1184) which were treated with different concentrations of F. deltoidea leaf extract. The data of wound closure were collected at time intervals of 0, 6, 12 and 24 hours and analyzed using ImageJ™ software. MTT assay revealed that the F. deltoidea leaf extract could induce cell proliferation at a dose dependent manner. F. deltoidea leaf extract significantly accelerated the wound closure process in comparison to cells treated with ascorbic acid and untreated cells in scratch assay. Following 24 hours of incubation, cells treated with 50 mg/mL of F. deltoidea leaf extract showed remarkable proliferative and wound closure effect with 143.67% and 5.96%, respectively as compared to other concentrations. Collectively, these findings suggested that F. deltoidea leaf extract possesses wound healing potential and may be useful for the development of efficient wound healing drug.

scholarly journals FORMULATION AND EVALUATION OF WOUND HEALING GEL OF WHITE LEADTREE (LEUCAENA LEUCOCEPHALA (LAM.) DE WIT.) LEAVES EXTRACT

2022 ◽  
pp. 275-280
Author(s):  
EVY FENNY VERONICA ◽  
RINI DWIASTUTI
Keyword(s):  
Wound Healing ◽  
Propylene Glycol ◽  
Leucaena Leucocephala ◽  
Wound Closure ◽  
Healing Process ◽  
Statistical Parameter ◽  
Physical Characteristics ◽  
Gelling Agent ◽  
Wound Healing Process ◽  
Wound Healing Activity

Objective: The aim of this research is to formulate and evaluate the wound healing gel of Leucaena leucocephala leaves extract. Methods: In this research, the extract of Leucaena leaves was formulated into wound healing gel by using a variation of the concentration of Carbopol as a gelling agent and Propylene glycol as a humectant. Afterward, the gel's physical properties (pH, viscosity, spreadability), stability, and sterility were tested. The wound healing activity was evaluated by making excision wounds on the Wistar Albino rat's back, and then the gels were applied to the wound every day. The wound's size was measured and counted as the percentage of wound closure. Results: The result showed that Formula 4 (contains 1.5% of Carbopol and 12% of Propylene glycol) has the best physical characteristics and wound healing activity. Formula 4 showed 100% wound closure on the 11th day of the treatment, while the negative control only reached 49.12%. The statistical parameter with the p-value<0.05 stated that they are significantly different. Conclusion: This research demonstrated that gel with Leucaena leaf extract has good physical characteristics, and it can significantly improve the wound healing process.

scholarly journals Wound Healing Activity of Ethanolic Extract Gel of Tawas Ut Tuber (Ampelocissus rubiginosa L.) in Incisional Model Wistar Rats

2018 ◽  
Vol 23 (1) ◽  
pp. 30 ◽  
Author(s):  
Khoerul Anwar ◽  
Dewita Fitri Widodo ◽  
Nurlely Nurlely ◽  
Liling Triyasmono ◽  
Sudarsono Sudarsono ◽  
...  
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wistar Rats ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Average Percentage ◽  
Wound Length ◽  
Wound Healing Activity

Tawas ut tuber (Ampelocissus rubiginosa Lauterb.) is empirically used in wound healing by Dayak community. The present study was undertaken to assess wound healing activity of gel from ethanol extract of A. rubiginosa tuber using parameters of the closure of wound length, tensile strength, and histopathological observations. This study used twenty-five male Wistar rats divided into five groups: positive control (Bioplacenton®), negative control (placebo gel), and three of testing groups with gel extract (concentrations of 1.5%, 2.0%, and 2.5%). Wound healing activity was evaluated to 4 cm length and ± 2 mm depth incision wound model on the back skin of rats. The measurement of wound length was observed on the day of 4th, 8th, 12th, 16th, and 20th used quantitative analyze. On the day of 20th, animal was anaesthetized and the skin has been taken for tensile strength evaluation and histopathological observations. This study showed that group treated with gel extract at the concentration of 2.5% experienced higher wound healing activity with average percentage of wound closure of 99.00% ± 0.16, tensile strength 3.8541 gram/mm2, and proved with the re-epithelization, neocapillarization, and increase collagen density appeared in histopathological observations. Based on this study, gel from ethanol extract of A. rubiginosa tuber possesses wound healing activity.

scholarly journals The wound healing ability of the prepared ointment incorporating herbal Extracts

2019 ◽  
Vol 9 (1) ◽  
pp. 5-8
Author(s):  
Irfana Asma S ◽  
Ravichandran S ◽  
Bhavani J ◽  
Satheesh Kumar D ◽  
Chandrasekaran A R ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Body ◽  
The Body ◽  
Distilled Water ◽  
Herbal Extracts ◽  
Physical Damage ◽  
Standard Drug ◽  
Physical Injuries ◽  
Ointment Formulation ◽  
Wound Healing Activity

Physical injuries or wounds are the physical damage to the skin. The human body naturally possesses the wound healing ability to heal physical damages. The wound healing was initiated in the human body in various mechanisms, and it occurs in three phases. The plants like Musa and Tridax have been evaluated for the wound healing activity, and the extracts were seen toxic to the body too. So in this research, the ointment formulation was prepared using the extracts of the two plants that are extracted with water as the solvent. The polyherbal ointment was prepared using the herbal extracts that were extracted from the parts of Tridax and Musa using the distilled water as a solvent. The prepared ointments showed a better activity compared to the crude extracts and the standard drug too.

Antimicrobial and Wound Healing Potential of Dietary Flavonoid Naringenin

2019 ◽  
Vol 9 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Ankita Tripathi ◽  
Himani Awasthi ◽  
Dan Bahadur Rokaya ◽  
Dipti Srivastava ◽  
Vivek Srivastava
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Tensile Strength ◽  
Agar Plate ◽  
Wound Contraction ◽  
Healing Index ◽  
Phenolic Constituents ◽  
Healing Property ◽  
Wound Healing Activity ◽  
Wound Healing Property

Background: Naringenin belongs to the class flavanones, and chemically it is known as 5,7-dihydroxy-2-(4-hydroxyphenyl) chroman-4-one. It is one of the dietary poly-phenolic constituents of the citrus fruits and vegetables. Naringenin possesses excellent antioxidant, antiinflammatory, and antimicrobial activities. These activities promote wound healing activity. Naringenin has been incorporated in the gel base to be applied on the wounded skin. The wound healing property of naringenin gel has not been studied extensively in the literature to date. </P><P> Objective: The present study aims to unveil the wound healing property of naringenin gel formulation on excision and incision model. </P><P> Materials and Methods: 0.5% and 1% naringenin was incorporated in a carbopol gel base formulation and evaluated for physicochemical parameters. Incision and excision wound models were used to study wound contraction, tensile strength, healing index, the period of epithelization and histopathological parameters. Antimicrobial activity was performed using agar plate method. Results: Naringenin (0.5% and 1%) treatment shows a significant increase in the percentage of wound contraction, good healing index and shorter epithelization period. Histopathology of naringenin gel treated wound tissue showed more fibroblasts, less macrophage infiltration, and wellformed collagen fibers as compared to control. In incision wound model, the animals were treated with 0.5% and 1% naringenin gel for nine days and showed a significant increase in the tensile strength. Naringenin exerts antimicrobial activity against fungus and E. coli. Conclusion: The present study explored potent wound healing activity of naringenin gels.

scholarly journals Curcumin-Loaded Chitosan/Gelatin Composite Sponge for Wound Healing Application

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Van Cuong Nguyen ◽  
Van Boi Nguyen ◽  
Ming-Fa Hsieh
Keyword(s):  
Wound Healing ◽  
Chemical Structure ◽  
Wound Closure ◽  
Absorption Capacity ◽  
Water Absorption Capacity ◽  
Structure And Morphology ◽  
Wound Model ◽  
Wound Healing Activity

Three composite sponges were made with 10% of curcumin and by using polymers, namely, chitosan and gelatin with various ratios. The chemical structure and morphology were evaluated by FTIR and SEM. These sponges were evaluated for water absorption capacity, antibacterial activity,in vitrodrug release, andin vivowound healing studies by excision wound model using rabbits. Thein vivostudy presented a greater wound closure in wounds treated with curcumin-composite sponge than those with composite sponge without curcumin and untreated group. These obtained results showed that combination of curcumin, chitosan and gelatin could improve the wound healing activity in comparison to chitosan, and gelatin without curcumin.

scholarly journals Film-Forming Spray of Water-Soluble Chitosan Containing Liposome-Coated Human Epidermal Growth Factor for Wound Healing

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5326
Author(s):  
Abd. Kakhar Umar ◽  
Sriwidodo Sriwidodo ◽  
Iman Permana Maksum ◽  
Nasrul Wathoni
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Wound Dressing ◽  
Wound Closure ◽  
Water Soluble ◽  
Film Forming ◽  
Epidermal Growth ◽  
Film Method ◽  
Wound Healing Activity

Human epidermal growth factor (hEGF) has been known to have excellent wound-healing activity. However, direct application to the wound area can lead to low hEGF bioavailability due to protease enzymes or endocytosis. The use of liposomes as coatings and carriers can protect hEGF from degradation by enzymes, chemical reactions, and immune reactions. Sustained release using a matrix polymer can also keep the levels of hEGF in line with the treatment. Therefore, this study aimed to develop a film-forming spray of water-soluble chitosan (FFSWSC) containing hEGF-liposomes as a potential wound dressing. The hEGF-liposomes were prepared using the hydration film method, and the preparation of the FFSWSC was achieved by the ionic gelation method. The hydration film method produced hEGF-liposomes that were round and spread with a Z-average of 219.3 nm and encapsulation efficiency of 99.87%, whereas the film-forming solution, which provided good sprayability, had a formula containing 2% WSC and 3% propylene glycol with a viscosity, spray angle, droplet size, spray weight, and occlusion factor of 21.94 ± 0.05 mPa.s, 73.03 ± 1.28°, 54.25 ± 13.33 µm, 0.14 ± 0.00 g, and 14.57 ± 3.41%, respectively. The pH, viscosity, and particle size of the FFSWSC containing hEGF-liposomes were stable during storage for a month in a climatic chamber (40 ± 2 °C, RH 75 ± 5%). A wound healing activity test on mice revealed that hEGF-liposomes in FFSWSC accelerated wound closure significantly, with a complete wound closure on day 6. Based on the findings, we concluded that FFSWSC containing hEGF-liposomes has the potential to be used as a wound dressing.

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