Renoprotective effect of Caralluma fimbriata against high-fat diet-induced oxidative stress in Wistar rats

The study investigated the influence of chia consumption on inflammation, oxidative stress, and lipid profiles in female ovariectomized rats fed a high-fat diet.

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Protective role of melatonin against adipose-hepatic metabolic comorbidities in experimentally induced obese rat model

PLoS ONE ◽

10.1371/journal.pone.0260546 ◽

2021 ◽

Vol 16 (12) ◽

pp. e0260546

Author(s):

Mary J. Obayemi ◽

Christopher O. Akintayo ◽

Adesola A. Oniyide ◽

Ayodeji Aturamu ◽

Olabimpe C. Badejogbin ◽

...

Keyword(s):

Oxidative Stress ◽

Insulin Resistance ◽

Food Intake ◽

High Fat Diet ◽

Wistar Rats ◽

Liver Lipid ◽

Control Group ◽

Metabolic Dysfunction ◽

High Fat ◽

Male Wistar Rats

Background Adipose and hepatic metabolic dysfunctions are critical comorbidities that also aggravate insulin resistance in obese individuals. Melatonin is a low-cost agent and previous studies suggest that its use may promote metabolic health. However, its effects on some comorbidities associated with obesity are unknown. Herein, we investigated the hypothesis that melatonin supplementation would attenuate adipose-hepatic metabolic dysfunction in high fat diet (HFD)-induced obesity in male Wistar rats. Materials and methods Twenty-four adult male Wistar rats (n = 6/group) were used: Control group received vehicle (normal saline), obese group received 40% high fat diet, melatonin-treated group received 4 mg/kg of melatonin, and obese plus melatonin group received 40% HFD and melatonin. The treatment lasted for 12 weeks. Results HFD caused increased food intake, body weight, insulin level, insulin resistance and plasma and liver lipid but decreased adipose lipid. In addition, HFD also increased plasma, adipose and liver malondialdehyde, IL-6, uric acid and decreased Glucose-6-phosphate dehydrogenase, glutathione, nitric oxide and circulating obestatin concentration. However, these deleterious effects except food intake were attenuated when supplemented with melatonin. Conclusion Taken together, the present results indicate that HFD exposure causes adipose-hepatic metabolic disturbance in obese animals, which are accompanied by oxidative stress and inflammation. In addition, the present results suggest that melatonin supplementation attenuates adipose-hepatic metabolic dysfunction, accompanying obesity by suppression of oxidative stress/inflammation-dependent mechanism and increasing circulating obestatin.

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High-Fat Diet and Alcohol Intake Promotes Inflammation and Impairs Skin Wound Healing in Wistar Rats

Mediators of Inflammation ◽

10.1155/2018/4658583 ◽

2018 ◽

Vol 2018 ◽

pp. 1-12 ◽

Cited By ~ 6

Author(s):

Daiane Figueiredo Rosa ◽

Mariáurea Matias Sarandy ◽

Rômulo Dias Novaes ◽

Mariella Bontempo Freitas ◽

Maria do Carmo Gouveia Pelúzio ◽

...

Keyword(s):

Oxidative Stress ◽

Wound Healing ◽

High Fat Diet ◽

Wistar Rats ◽

Alcohol Intake ◽

Skin Wound ◽

Chow Diet ◽

High Fat ◽

Skin Wound Healing ◽

Standard Chow Diet

The wound-healing process is complex and remains a challenging process under the influence of several factors, including eating habits. As improper diets may lead to disorders such as dyslipidemia, insulin resistance, and chronic inflammation, potentially affecting the tissue ability to heal, we decided to investigate the effect of a high-fat diet and alcohol intake on the inflammatory process and skin wound healing in Wistar rats. Male rats (n=30) were individually housed in cages with food and water ad libitum (registration number 213/2014). After anesthesia, at day 40, three circular wounds (12 mm diameter) were made on the back of each animal, which were then randomly assorted into five treatment groups: C1 (control 1)—water via gavage and standard chow diet; C2 (control 2)—water (no gavage) and standard chow diet; AL (alcohol)—water (no gavage) and alcohol (40%) via gavage and standard chow diet; HF (high fat)—water (no gavage) and high-fat diet (50%); and HF + AL (alcohol/high fat)—water (no gavage), alcohol (40%) via gavage, and high-fat diet. Animals were treated for 61 days. Every seven days, the area and the rate of wound contraction were evaluated. Tissue samples were removed for histopathological analysis and biochemical analyses. Our results showed that wound contraction was not complete in the HF + AL rats. Two specific indices of wound-healing impairment (total cell number and levels of the inflammatory cytokine TGF-β) were increased in the HF + AL rats. We also observed decreased type I and III collagen fibers in the HF, AL, and HF + AL groups and increased oxidative stress markers in the same groups. We suggest that a high-fat diet combined with alcohol intake contributed to delayed skin wound healing through increase of the inflammatory phase and promoting oxidative stress, which may have led to morphological alterations and impaired matrix remodeling.

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Amla (Emblica officinalis) improves hepatic and renal oxidative stress and the inflammatory response in hypothyroid female wistar rats fed with a high-fat diet

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2017-0116 ◽

2018 ◽

Vol 29 (2) ◽

pp. 175-184 ◽

Cited By ~ 5

Author(s):

P. Rajaa Muthu ◽

Zachariah Bobby ◽

P. Sankar ◽

V. Vickneshwaran ◽

Sajini Elizabeth Jacob

Keyword(s):

Oxidative Stress ◽

Inflammatory Response ◽

High Fat Diet ◽

Wistar Rats ◽

Molecular Techniques ◽

Emblica Officinalis ◽

High Fat ◽

Tnf Α ◽

Female Wistar Rats ◽

Liver And Kidney

AbstractBackground:We investigated the protective effects of amla (Emblica officinalis) on the pathogenesis of oxidative stress (OS) and inflammatory response in hypothyroid rats fed with a high-fat diet (HFD) as an experimental model of hypothyroidism (HT) with obesity.Methods:A total of 80 female wistar rats (5-months-old) were divided into eight different groups. Propylthiouracil (PTU) and HFD were used to induce the experimental HT and obesity, respectively. The euthyroid and hypothyroid rats were fed either normal chow or HFD with and without amla extract (AE, 100 mg/kg bw/day) for 6 weeks. The blood and tissues, liver and kidney OS and inflammatory parameters were studied using appropriate biochemical and molecular techniques.Results:PTU and HFD per se caused OS and inflammatory response as evidenced by increased plasma MDA, TNF-α, CRP and GPx in association with decreased levels of TAS and reduced glutathione (GSH). The proteomic analysis revealed that the expressions of pERK, pP38, TNF-α, IL6, COX2 and NOX-4 were up-regulated in the liver and kidney of these rats. In addition, all these metabolic derangements were further augmented when HT was followed by the addition of HFD. This suggested that there was a synergism between HT and the intake of HFD on the development of OS and inflammatory response.Conclusions:The treatment with amla fruit extract significantly restored the redox imbalance and inflammatory signaling and ameliorated OS and inflammatory response, suggesting the use of this natural compound as an alternative remedy or adjuvant for the management of metabolic complications concomitant with HT.

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The Impact of High-Fat Diet on Mitochondrial Function, Free Radical Production, and Nitrosative Stress in the Salivary Glands of Wistar Rats

Oxidative Medicine and Cellular Longevity ◽

10.1155/2019/2606120 ◽

2019 ◽

Vol 2019 ◽

pp. 1-15 ◽

Cited By ~ 20

Author(s):

Anna Zalewska ◽

Dominika Ziembicka ◽

Małgorzata Żendzian-Piotrowska ◽

Mateusz Maciejczyk

Keyword(s):

Oxidative Stress ◽

Free Radical ◽

High Fat Diet ◽

Wistar Rats ◽

Nitrosative Stress ◽

Ros Production ◽

Parotid Glands ◽

High Fat ◽

Free Radical Production ◽

Radical Production

Oxidative stress plays a crucial role in the salivary gland dysfunction in insulin resistance; however, the cause of increased free radical formation in these conditions is still unknown. Therefore, the aim of the study was to investigate the effect of high-fat diet (HFD) on the mitochondrial respiratory system, prooxidant enzymes, ROS production, and nitrosative/oxidative stress in the submandibular and parotid glands of rats. The experiment was performed on male Wistar rats divided into two groups (n=10): control and HFD. The 8-week feeding of HFD affects glucose metabolism observed as significant increase in plasma glucose and insulin as well as HOMA-IR as compared to the control rats. The activity of mitochondrial Complex I and Complex II+III was significantly decreased in the parotid and submandibular glands of HFD rats. Mitochondrial cytochrome c oxidase (COX) activity and the hydrogen peroxide level were significantly increased in the parotid and submandibular glands of the HFD group as compared to those of the controls. HFD rats also showed significantly lower reduced glutathione (GSH) and reduced : oxidized glutathione (GSH : GSSG) ratio, as well as a higher GSSG level in the parotid glands of HFD rats. The activity of NADPH oxidase, xanthine oxidase, and levels of oxidative/nitrosative stress (malonaldehyde, nitric oxide, nitrotyrosine, and peroxynitrite) and inflammation/apoptosis (interleukin-1βand caspase-3) biomarkers were statistically elevated in the HFD group in comparison to the controls. HFD impairs mitochondrial function in both types of salivary glands by enhancing ROS production, as well as stimulating inflammation and apoptosis. However, free radical production, protein nitration, and lipid peroxidation were more pronounced in the parotid glands of HFD rats.

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Grape Juice Consumption with or without High Fat Diet during Pregnancy Reduced the Weight Gain and Improved Lipid Profile and Oxidative Stress Levels in Liver and Serum from Wistar Rats

Beverages ◽

10.3390/beverages4040078 ◽

2018 ◽

Vol 4 (4) ◽

pp. 78 ◽

Cited By ~ 2

Author(s):

Luciana Gonçalves ◽

Gabrielli Bortolato ◽

Ruben Dario Braccini Neto ◽

Marina Rocha Frusciante ◽

Claudia Funchal ◽

...

Keyword(s):

Oxidative Stress ◽

Weight Gain ◽

High Fat Diet ◽

Wistar Rats ◽

Control Diet ◽

Grape Juice ◽

Free Access ◽

Tbars Level ◽

High Fat ◽

And Oxidative Stress

The aim of this study was to evaluate the effects of high fat diet with or without grape juice during the pregnancy on gestational weight gain, biochemical parameters, and oxidative stress in plasma and liver from Wistar rats. Forty-nine rats were divided into four groups: control diet group (CD), high fat diet (HFD), grape juice and control diet (PGJCD), and grape juice and high fat diet (PGJHFD). During the treatment the weight gain of the rats was tracked. They had free access to their respective diets during 42 days of treatment. After offspring weaning, the mother rats were euthanized and blood and liver were collected. The high fat diet increased the total cholesterol and triglycerides serum levels as well as carbonyl levels in the liver, however this diet reduced the high-density lipoprotein (HDL) and urea levels in serum. Grape juice consumption reduced gestational body weight gain. In liver, the juice consumption increased sulfhydryl levels and reduced the superoxide dismutase (SOD) activity and TBARS level, in serum the consumption reduced aspartate aminotransferase (AST) and TBARS. We can conclude that the consumption of a diet rich in fat can promotes harmful effects on health during pregnancy, however the consumption of grape juice seems to be an important alternative to prevent oxidative damages and to promote the improvement of health.

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Metabolic and oxidative stress markers in Wistar rats after 2 months on a high-fat diet

Diabetology & Metabolic Syndrome ◽

10.1186/1758-5996-6-130 ◽

2014 ◽

Vol 6 (1) ◽

pp. 130 ◽

Cited By ~ 23

Author(s):

Nathalie Auberval ◽

Stéphanie Dal ◽

William Bietiger ◽

Michel Pinget ◽

Nathalie Jeandidier ◽

...

Keyword(s):

Oxidative Stress ◽

High Fat Diet ◽

Wistar Rats ◽

Oxidative Stress Markers ◽

High Fat ◽

Stress Markers ◽

And Oxidative Stress

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Oxidative stress and mitochondrial uncoupling protein 2 expression in hepatic steatosis induced by exposure to xenobiotic DDE and high fat diet in male Wistar rats

PLoS ONE ◽

10.1371/journal.pone.0215955 ◽

2019 ◽

Vol 14 (4) ◽

pp. e0215955 ◽

Cited By ~ 5

Author(s):

Vincenzo Migliaccio ◽

Rosaria Scudiero ◽

Raffaella Sica ◽

Lillà Lionetti ◽

Rosalba Putti

Keyword(s):

Oxidative Stress ◽

Hepatic Steatosis ◽

High Fat Diet ◽

Wistar Rats ◽

Uncoupling Protein ◽

Uncoupling Protein 2 ◽

High Fat ◽

Mitochondrial Uncoupling ◽

Mitochondrial Uncoupling Protein ◽

Male Wistar Rats

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Thymoquinone ameliorates NLRP3-mediated inflammation in the pancreas of albino Wistar rats fed ethanol and high-fat diet

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2014-0109 ◽

2015 ◽

Vol 26 (6) ◽

Cited By ~ 2

Author(s):

Suguna Periyanayagam ◽

Geetha Arumugam ◽

Aruna Ravikumar ◽

Vijaiyan Siva Ganesan

Keyword(s):

Oxidative Stress ◽

Protein Expression ◽

Mrna Expression ◽

High Fat Diet ◽

Wistar Rats ◽

Inflammatory Activity ◽

High Fat ◽

Caspase 1 ◽

Tnf Α ◽

Anti Inflammatory

AbstractInflammasomes are protein complexes that mediate the process of inflammation and tissue injury by regulating the level of cytokine production. Pancreatitis is a major gastrointestinal disorder characterized by painful inflammation in the pancreas. The aim of this study was to evaluate whether thymoquinone (TQ) exerts anti-inflammatory activity by influencing the expression of the apoptosis-associated speck-like protein (ASC) complex of NOD-like receptor pyrin domain containing 3 (NLRP3) inflammasomes in rats subjected to experimental pancreatitis.Male albino Wistar rats were randomly separated into four groups. Rats in groups 1 and 2 were fed with a normal diet for 90 days, and rats in groups 3 and 4 were administered with ethanol (EtOH) 8–12 g/kg/day orally and fed with a high-fat diet (HFD) for 90 days. In addition, rats in groups 2 and 4 were administered with 100 mg/kg body weight of TQ from the 31st day. The serum lipase (L)/amylase (A) ratio; the oxidative stress markers; the GSH/GSSG ratio; the mRNA expression of ASC, caspase-1, IL-1β, IL-18, and TNF-α; and the protein expression of ASC and caspase-1 in the pancreas were assessed.We observed a significant increase in the serum L/A ratio and oxidative stress, a decrease in the GSH/GSSG ratio, and a GST activity in EtOH- and HFD-fed rats. The mRNA expression of IL-1β, IL-18, and TNF-α was significantly reduced in TQ-coadministered rats than that in EtOH- and HFD-fed rats. The upregulation of mRNA and the protein expression of ASC and caspase-1 were significantly reduced in TQ-coadministered rats.TQ exerts the anti-inflammatory activity probably by downregulating the ASC expression to minimize the maturation of proinflammatory cytokines.

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