LB726 The combination of 0.5% retinol with a naturally derived TRPV 1 antagonist and anti-inflammatory botanical extract helps mitigate retinoid-induced irritation

The anti-inflammatory and antioxidant role of Thykamine, a botanical extract of thylakoides obtained from spinach leaves, has been investigated in animal and cellular models. The oxidative properties have been proven by inhibiting NO production (>98%) in J774A.1 cells and by protecting a linoelic acid emulsion subjected to lipid peroxidation caused by AAPH. Thykamine injected intraperitoneally to rats reduced the inflammatory process of (TNBS)-induced colitis and carrageenan-induced paw edema. As neutrophils are the first cells to migrate to inflammatory sites, the influence of Thykamine on the primary neutrophil functions were studied. Thykamine dose-dependent reduced neutrophil chemiotaxis, phagocytosis, and degranulation. No change in the release of LDH by neutrophils on Thykamine was recorded. Thykamine inhibited by 85% the neutrophil production of O2−. A superoxide recovery activity was observed on a zymography demonstrating a SOD-like enzyme on Thykamine extracts. Spontaneous fluorescence provided by carotenoid and chlorophyll pigments (488/675 nm) detected Thykamine on the surface, in the cytoplasm (mainly central where Golgi are present) and weakly in the nucleus of neutrophils. The results argue that SOD and pigments found in Thykamine are part of its antioxidant and anti-inflammatory properties shown in in vivo and in vitro models of inflammation.

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Primary Evaluation of Anti-Inflammatory Effect of a Botanical Extract-Based Blend in a Haze Infected Cell Model

Current Developments in Nutrition ◽

10.1093/cdn/nzaa045_065 ◽

2020 ◽

Vol 4 (Supplement_2) ◽

pp. 432-432

Author(s):

Jihong Lu ◽

Lilan Jin ◽

Lu Deng ◽

Yixiao Pan ◽

Hai Wang ◽

...

Keyword(s):

Gene Expression ◽

Adverse Effects ◽

Tight Junction ◽

Scaffolding Protein ◽

Inflammatory Factors ◽

Airborne Pollutants ◽

Tnf Α ◽

Junction Protein ◽

Anti Inflammatory ◽

Botanical Extract

Abstract Objectives Exposure to air pollution has been associated with throat discomfort, cough, pulmonary disease, and even lung cancer. We developed a botanical extract-based blend with the goal of ameliorating some of the adverse effects of airborne pollutants. This blend consisted of broccoli seed (BS, Brasssica oleracea) extract, Luo Han Guo (LH G, Siraitia grovenorii) extract, and Honeysuckle Flower Bud (HFB, Lonicera japonica) extract. BS extract is rich in Sulforaphane glucosinolate and is reported to promote healthy inflammatory balance as well as to regulate oxidative stress and DNA-damaging substances. LHG has been used as a traditional medicine for the treatment of pharyngitis, pharyngeal pain, as well as an antitussive remedy in China. HFB is a traditional herbal medicine widely used in East Asia as an anti-inflammatory and anti-oxidative agent. Methods In order to evaluate the effectiveness of this putative airborne pollutant defense blend, human alveolar basal epithelial cells in adenocarcinoma (A549) were treated by exposing total culture medium (TCMed, to 0.02 mg/mL of haze particles (HP). Gene expression of inflammatory factors IL-1β, IL-8, TNF-α, INF-γ, and cell tight junction proteins were detected by qPCR at 0.5, 1, 2, and 6H when treated with 0.02 mg/mL HP TCMed alone (group H), 0.02 mg/mL HP TCMed + 5 mg/mL of LHG + HFB blend (LHG: HFB 1:10) +0.01 mg/mL of L-sulforaphane (group HT). Results We found that gene expression of IL-1β, IL-8 and TNF-α were significantly decreased in HT at 1, 2, and 6 H treatment as compared to the F group. Gene expression of tight junction protein OCLN and ZO-1, which is a scaffolding protein, were significantly increased in HT as compared to the F group. Conclusions This botanical extract blend regulates inflammatory factors and balances cell transmembrane stability in a primary cell haze infection model and therefore may be useful as a preventive formulation to help alleviate the adverse effects of airborne pollutants. Funding Sources Nu Skin Enterprises.

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