Prevalence, sequence type, and quinolone resistance of Neisseria lactamica carried in children younger than 15 years in Shanghai, China

ABSTRACT We describe 66 ciprofloxacin-nonsusceptible Streptococcus pyogenes isolates recovered from colonized and infected children. The ParC S79A substitution was frequent and associated with the emm6/sequence type 382 (emm6/ST382) lineage. The ParC D83G substitution was detected in two isolates (emm5/ST99 and emm28/ST52 lineages). One isolate (emm89/ST101) had no quinolone resistance-determining region codon substitutions or other resistance mechanisms. Five of 66 isolates were levofloxacin resistant. Although fluoroquinolones are not used in children, they may be putative disseminators of fluoroquinolone-nonsusceptible strains in the community.

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Genetic Diversity and Quinolone Resistance in Campylobacter jejuni Isolates from Poultry in Senegal

Applied and Environmental Microbiology ◽

10.1128/aem.72.5.3309-3313.2006 ◽

2006 ◽

Vol 72 (5) ◽

pp. 3309-3313 ◽

Cited By ~ 34

Author(s):

Alfred Dieudonnï¿½ Kinana ◽

Eric Cardinale ◽

Fatou Tall ◽

Ibrahim Bahsoun ◽

Jean-Marie Sire ◽

...

Keyword(s):

Genetic Diversity ◽

Campylobacter Jejuni ◽

Human Disease ◽

Multilocus Sequence Typing ◽

Sequence Type ◽

Quinolone Resistance ◽

Resistance Phenotype ◽

Mechanism Of Resistance ◽

Unique Clone

ABSTRACT We used the multilocus sequence typing (MLST) method to evaluate the genetic diversity of 46 Campylobacter jejuni isolates from chickens and to determine the link between quinolone resistance and sequence type (ST). There were a total of 16 ST genotypes, and the majority of them belonged to seven clonal complexes previously identified by using isolates from human disease. The ST-353 complex was the most common complex, whereas the ST-21, ST-42, ST-52, and ST-257 complexes were less well represented. The resistance phenotype varied for each ST, and the Thr-86-Ile substitution in the GyrA protein was the predominant mechanism of resistance to quinolone. Nine of the 14 isolates having the Thr-86-Ile substitution belonged to the ST-353 complex. MLST showed that the emergence of quinolone resistance is not related to the diffusion of a unique clone and that there is no link between ST genotype and quinolone resistance. Based on silent mutations, different variants of the gyrA gene were shown to exist for the same ST. These data provide useful information for understanding the epidemiology of C. jejuni in Senegal.

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Cooccurrence of NDM-1, ESBL, RmtC, AAC(6′)-Ib, and QnrB in Clonally Related Klebsiella pneumoniae Isolates Together with Coexistence of CMY-4 and AAC(6′)-Ib in Enterobacter cloacae Isolates from Saudi Arabia

BioMed Research International ◽

10.1155/2019/6736897 ◽

2019 ◽

Vol 2019 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Mohamed H. Al-Agamy ◽

Taghrid S. El-Mahdy ◽

Hesham H. Radwan ◽

Laurent Poirel

Keyword(s):

Escherichia Coli ◽

Saudi Arabia ◽

16S Rrna ◽

Klebsiella Pneumoniae ◽

High Resistance ◽

Enterobacter Cloacae ◽

Sequence Type ◽

Quinolone Resistance ◽

Synergy Test ◽

16S Rrna Methylase

The aim of this study was to investigate the mechanisms responsible for resistance to antimicrobials in a collection of enterobacterial isolates recovered from two hospitals in Saudi Arabia. A total of six strains isolated from different patients showing high resistance to carbapenems was recovered in 2015 from two different hospitals, with four being Klebsiella pneumoniae and two Enterobacter cloacae. All isolates except one K. pneumoniae were resistant to tigecycline, but only one K. pneumoniae was resistant to colistin. All produced a carbapenemase according to the Carba NP test, and all were positive for the EDTA-disk synergy test for detection of MBL. Using PCR followed by sequencing, the four K. pneumoniae isolates produced the carbapenemase NDM-1, while the two E. cloacae isolates produced the carbapenemase VIM-1. Genotyping analysis by Multilocus Sequence Typing (MLST) showed that three out of the four K. pneumoniae isolates were clonally related. They had been recovered from the same hospital and belonged to Sequence Type (ST) ST152. In contrast, the fourth K. pneumoniae isolate belonged to ST572. Noticeably, the NDM-1-producing K. pneumoniae additionally produced an extended-spectrum ß-lactamase (ESBL) of the CTX-M type, together with OXA-1 and TEM-1. Surprisingly, the three clonally related isolates produced different CTX-M variants, namely, CTX-M-3, CTX-M-57, and CTX-M-82, and coproduced QnrB, which confers quinolone resistance, and the 16S rRNA methylase RmtC, which confers high resistance to all aminoglycosides. The AAC(6′)-Ib acetyltransferase was detected in both K. pneumoniae and E. cloacae. Mating-out assays using Escherichia coli as recipient were successful for all isolates. The blaNDM-1 gene was always identified on a 70-kb plasmid, whereas the blaVIM-1 gene was located on either a 60-kb or a 150-kb plasmid the two E. cloacae isolates, respectively. To the best of our knowledge, this is the first report of the coexistence of an MBL (NDM-1), an ESBL (CTX-M), a 16S rRNA methylase (RmtC), an acetyltransferase (AAC[6′]-Ib), and a quinolone resistance enzyme (QnrB) in K. pneumoniae isolates recovered from different patients during an outbreak in a Saudi Arabian hospital.

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Ciprofloxacin-Resistant Salmonella enterica Serovar Kentucky ST198 in Broiler Chicken Supply Chain and Patients, China, 2010–2016

Microorganisms ◽

10.3390/microorganisms8010140 ◽

2020 ◽

Vol 8 (1) ◽

pp. 140 ◽

Cited By ~ 2

Author(s):

Zhiying Xiong ◽

Shaojun Wang ◽

Yumei Huang ◽

Yuan Gao ◽

Haiyan Shen ◽

...

Keyword(s):

Supply Chain ◽

Salmonella Enterica ◽

Broiler Chicken ◽

Genomic Island ◽

Public Health Problem ◽

Pulsed Field Gel Electrophoresis ◽

Sequence Type ◽

Quinolone Resistance ◽

Zoonotic Pathogen ◽

Ciprofloxacin Resistance

Salmonella enterica serovar Kentucky (S. Kentucky) sequence type 198 has emerged as a global zoonotic pathogen. We explored Salmonella enterica serovar Kentucky ST198 samples from the broiler chicken supply chain and patients between 2010 and 2016. Here, we collected 180 S. Kentucky isolates from clinical cases and the poultry supply chain. We performed XbaI pulsed-field gel electrophoresis and multilocus sequence typing. We assessed mutations in the quinolone resistance-determining regions and screened for the presence of the Salmonella genomic island 1 (SGI1). We determined that 63 (35.0%) of the 180 isolates were S. Kentucky ST198. Chinese strains of S. Kentucky ST198 have a high transmission of ciprofloxacin resistance (38/63, 60.3%) and a high risk of multidrug resistance. The quinolone resistance of the S. Kentucky ST198 strain found in China may be due to mutations in its quinolone resistance-determining region. Our study firstly revealed that ciprofloxacin-resistant S. Kentucky ST198 strains can undergo cross-host transmission, thereby causing a serious foodborne public health problem in China.

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Complete DNA Sequence of an IncM1 Plasmid Bearing the Novel qnrE1 Plasmid-Mediated Quinolone Resistance Variant and blaCTX-M-8 from Klebsiella pneumoniae Sequence Type 147

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00592-17 ◽

2017 ◽

Vol 61 (9) ◽

Cited By ~ 6

Author(s):

Marcos P. V. Cunha ◽

Yame M. Davies ◽

Louise Cerdeira ◽

Milena Dropa ◽

Nilton Lincopan ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Dna Sequence ◽

Sequence Type ◽

Quinolone Resistance ◽

The Novel

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P077: Observations on incidence of quinolone resistance genes and their association with SHV genotypes and bacterial sequence type in a Klebsiella pneumoniae outbreak

Antimicrobial Resistance and Infection Control ◽

10.1186/2047-2994-2-s1-p77 ◽

2013 ◽

Vol 2 (S1) ◽

Author(s):

HH Balkhy ◽

S Aljohani ◽

A Almasood ◽

T Uzzaman

Keyword(s):

Klebsiella Pneumoniae ◽

Resistance Genes ◽

Sequence Type ◽

Quinolone Resistance ◽

Bacterial Sequence

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Long-Term Dissemination of CTX-M-5-Producing Hypermutable Salmonella enterica Serovar Typhimurium Sequence Type 328 Strains in Russia, Belarus, and Kazakhstan

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02506-14 ◽

2014 ◽

Vol 58 (9) ◽

pp. 5202-5210 ◽

Cited By ~ 7

Author(s):

Varvara K. Kozyreva ◽

Elena N. Ilina ◽

Maja V. Malakhova ◽

Alessandra Carattoli ◽

Ilya S. Azizov ◽

...

Keyword(s):

Salmonella Enterica ◽

Genetic Relatedness ◽

Single Point ◽

Point Mutations ◽

Sequence Type ◽

Quinolone Resistance ◽

Resistance Mutations ◽

Content Type ◽

Serovar Typhimurium

ABSTRACTIn this paper, we present evidence of long-term circulation of cefotaxime-resistant clonally relatedSalmonella entericaserovar Typhimurium strains over a broad geographic area. The genetic relatedness of 88 isolates collected from multiple outbreaks and sporadic cases of nosocomial salmonellosis in various parts of Russia, Belarus, and Kazakhstan from 1996 to 2009 was established by multilocus tandem-repeat analysis (MLVA) and multilocus sequence typing (MLST). The isolates belong to sequence type 328 (ST328) and produce CTX-M-5 β-lactamase, whose gene is carried by highly related non-self-conjugative but mobilizable plasmids. Resistance to nalidixic acid and low-level resistance to ciprofloxacin is present in 37 (42%) of the isolates and in all cases is determined by various single point mutations in thegyrAgene quinolone resistance-determining region (QRDR). Isolates of the described clonal group exhibit a hypermutable phenotype that probably facilitates independent acquisition of quinolone resistance mutations.

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